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Human Adenosylhomocysteinase/AHCY ELISA Kit (Colorimetric)

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ELISA: Human Adenosylhomocysteinase/AHCY ELISA Kit (Colorimetric) [NBP3-27192] - Standard Curve Reference
ELISA: Human Adenosylhomocysteinase/AHCY ELISA Kit (Colorimetric) [NBP3-27192] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Tissue homogenates, cell lysates and other biological fluids
Standard Curve Range
0.156 - 10 ng/mL (example only; lot dependent)
Sensitivity
0.069 ng/mL (example only; lot dependent)

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Human Adenosylhomocysteinase/AHCY ELISA Kit (Colorimetric) Summary

Description
Assay Length: 4.5 hours
Standard Curve Range
0.156 - 10 ng/mL (example only; lot dependent)
Sensitivity
0.069 ng/mL (example only; lot dependent)
Assay Type
Sandwich ELISA
Inter-Assay
%CV < 12 (example only; lot dependent)
Intra-Assay
%CV < 10 (example only; lot dependent)
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
AHCY

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Detection Reagent A
  2. Detection Reagent B
  3. Diluent Buffer
  4. Instruction manual
  5. Plate sealer for 96 wells
  6. Pre-coated 96T strip plate
  7. Standard
  8. Stop Solution
  9. TMB Substrate
  10. Wash Buffer (30 x concentrate)

Alternate Names for Human Adenosylhomocysteinase/AHCY ELISA Kit (Colorimetric)

  • Adenosylhomocysteinase
  • AdoHcyase
  • AHCY
  • EC 3.3.1.1
  • S-adenosylhomocysteine hydrolase
  • S-adenosyl-L-homocysteine hydrolase
  • SAHH
  • SAHHadoHcyase

Background

S-adenosylhomocysteine hydrolase catalyzes the reversible hydrolysis of S-adenosylhomocysteine (AdoHcy) to adenosine (Ado) and L-homocysteine (Hcy). Thus, it regulates the intracellular S-adenosylhomocysteine (SAH) concentration thought to be important for transmethylation reactions. Deficiency in this protein is one of the different causes of hypermethioninemia. S-adenosylhomocysteine hydrolase belongs to the adenosylhomocysteinase family.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Human Adenosylhomocysteinase/AHCY ELISA Kit (Colorimetric)
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Contact Information

Product PDFs

  SDS
  Human Adenosylhomocysteinase/AHCY ELISA Kit (Colorimetric) Manual
  PDF Datasheet
  Request CofA

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Bioinformatics

Gene Symbol AHCY