![Western Blot: Aconitase 2 Antibody [NBP1-52014] - (0.01ug/ml) staining of Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-Western-Blot-NBP1-52014-img0002.jpg "Western Blot: Aconitase 2 Antibody [NBP1-52014] - (0.01ug/ml) staining of Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
| Reactivity | Hu, Mu, Rt, Po, Bv, CaSpecies Glossary |
| Applications | WB, ELISA, IHC |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Concentration | 0.5 mg/ml |
| Immunogen | Peptide with sequence C-QHVDVSPTSQRLQ corresponding to internal region according to NP_001089.1. |
| Epitope | C-QHVDVSPTSQRLQ |
| Predicted Species | Mouse (100%), Rat (100%), Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | ACO2 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | IF: An anonymous customer has reported weak pixulate staining in the cytoplasm of HeLa WB: Approx. 90 kDa band observed in human heart lysates (calculated MW of 85.4 kDa band according to NP_001089.1). IHC-P: Human breast shows mitochondrial staining in the epithelial cells of the ducts. |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Immunogen affinity purified |
| Publication using NBP1-52014 | Applications | Species |
|---|---|---|
| Bota DA, Davies KJ. Lon protease preferentially degrades oxidizedmitochondrial aconitase by an ATP-stimulated mechanism. Nat Cell Biol 2002-09-01 [PMID: 12198491] |
Secondary Antibodies |
Isotype Controls |
Research Areas for Aconitase 2 Antibody (NBP1-52014)Find related products by research area.
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| Gene Symbol | ACO2 |
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| Uniprot |
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![Immunohistochemistry-Paraffin: Aconitase 2 Antibody [NBP1-52014] - (3.8ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Aconitase-2-Antibody-Immunohistochemistry-Paraffin-NBP1-52014-img0003.jpg "Immunohistochemistry-Paraffin: Aconitase 2 Antibody [NBP1-52014] - (3.8ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.")
![Complement Component C2 Antibody [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "Complement Component C2 Antibody [Unconjugated]")
