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AATF Antibody (2H6)

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Western Blot: AATF Antibody (2H6) [H00026574-M09] - Analysis of AATF expression in HeLa (Cat # L013V1).
Western Blot: AATF Antibody (2H6) [H00026574-M09] - Analysis of AATF expression in HeLa (Cat # L013V1).
Immunocytochemistry/ Immunofluorescence: AATF Antibody (2H6) [H00026574-M09] - Analysis of monoclonal antibody to AATF on HeLa cell. Antibody concentration 10 ug/ml
Sandwich ELISA: AATF Antibody (2H6) [H00026574-M09] - Detection limit for recombinant GST tagged AATF is 0.03 ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF
Clone
2H6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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AATF Antibody (2H6) Summary

Immunogen
AATF (AAH00591, 151 a.a. ~ 250 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. VQSISDFEKFTKGMDDLGSSEEEEDEESGMEEGDDAEDSQGESEEDRAGDRNSEDDGVVMTFSSVKVSEEVEKGRAVKNQIALWDQLLEGRIKLQKALLT
Specificity
AATF (2H6)
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
AATF
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Sandwich ELISA
  • Western Blot 1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.
Control
HeLa Whole Cell Lysate

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for AATF Antibody (2H6)

  • apoptosis antagonizing transcription factor
  • Apoptosis-antagonizing transcription factor
  • CHE-1
  • CHE1Rb-binding protein Che-1
  • DEDprotein AATF

Background

The protein encoded by this gene was identified on the basis of its interaction with MAP3K12/DLK, a protein kinase known to be involved in the induction of cell apoptosis. This gene product contains a leucine zipper, which is a characteristic motif of transcription factors, and was shown to exhibit strong transactivation activity when fused to Gal4 DNA binding domain. Overexpression of this gene interfered with MAP3K12 induced apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

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Control Lysate(s)

HeLa Whole Cell Lysate

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol AATF
Entrez
OMIM
Uniprot