Home » AAAS » AAAS Lysates » AAAS Overexpression Lysate
Novus Biologicals products are now on bio-techne.com

AAAS Overexpression Lysate

 Submit a Review

Images

 
(
Enlarge)
Western Blot: AAAS Overexpression Lysate (Adult Normal) [NBL1-07159] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for AAAS.
Western Blot: AAAS Overexpression Lysate (Adult Normal) [NBL1-07159] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for AAAS.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

AAAS Overexpression Lysate Summary

Description

AAAS Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC200154

Accession#: NM_015665

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
Gene
AAAS

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
Theoretical MW
59.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

Alternate Names for AAAS Overexpression Lysate

  • AAA
  • AAASb
  • achalasia, adrenocortical insufficiency, alacrimia (Allgrove, triple-A)
  • achalasia, adrenocortical insufficiency, alacrimia
  • ADRACALA
  • Adracalin
  • ALADIN
  • Allgrove, triple-A
  • DKFZp586G1624

Background

AAAS plays a role in the normal development of the peripheral and central nervous system

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

p97/VCP Antibody
NB100-1558
Western Blot p97/VCP Antibody
Western Blot p97/VCP Antibody
Western Blot p97/VCP Antibody
(12 Publications)
Species: Hu, Mu, Rt, Ze
Applications: ICC/IF, IHC, IHC-Fr, IP, WB
BRCAA1 Antibody
NBP1-26618
Immunohistochemistry BRCAA1 Antibody
Western Blot BRCAA1 Antibody
(1 Review)
(1 Publication)
Species: Hu
Applications: ChIP, IHC, IHC-P, IP, WB
MMP-9 [HRP]
DMP900
N/A MMP-9 [HRP]
N/A MMP-9 [HRP]
(219 Publications)
Species: Hu
Applications: ELISA
Elastin Antibody
(BA-4)
NB100-2076
Immunohistochemistry-Paraffin Elastin Antibody (BA-4)
Immunohistochemistry-Paraffin Elastin Antibody (BA-4)
Immunohistochemistry-Paraffin Elastin Antibody (BA-4)
(2 Reviews)
(11 Publications)
Species: Bv, Fe, Hu, Rb
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB
Spastin Antibody
(2F5)
H00006683-M02
Immunocytochemistry/ Immunofluorescence Spastin Antibody (2F5)
Western Blot Spastin Antibody (2F5)
Immunohistochemistry-Paraffin Spastin Antibody (2F5)
(1 Publication)
Species: Hu
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
IL-6 [HRP]
M6000B
N/A IL-6 [HRP]
N/A IL-6 [HRP]
N/A IL-6 [HRP]
(650 Publications)
Species: Mu
Applications: ELISA
MMP-2 [HRP]
MMP200
N/A MMP-2 [HRP]
N/A MMP-2 [HRP]
(101 Publications)
Species: Ca, Hu, Mu, Po, Rt
Applications: ELISA
Albumin Antibody
(188835) [Unconjugate ...
MAB1455
Simple Western Albumin Antibody (188835) [Unconjugated] - Serum
Immunocytochemistry Albumin Antibody (188835) [Unconjugated] - Serum
Immunohistochemistry Albumin Antibody (188835) [Unconjugated] - Serum
(3 Reviews)
(54 Publications)
Species: Hu
Applications: CyTOF-ready, Dual ISH-IHC, ICC, IHC, ICFlow, Simple Western, WB
Serpin
A8/Angiotensinogen An ...
NBP1-30027
Immunohistochemistry-Paraffin Serpin A8/Angiotensinogen Antibody - BSA Free
Western Blot Serpin A8/Angiotensinogen Antibody - BSA Free
Immunohistochemistry-Paraffin Serpin A8/Angiotensinogen Antibody - BSA Free
(11 Publications)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
TNF-alpha
[Unconjugated]
210-TA
SDS-PAGE TNF-alpha [Unconjugated]
Bioactivity TNF-alpha [Unconjugated]
SEC-MALS TNF-alpha [Unconjugated]
(3 Reviews)
(810 Publications)
Species: Hu
Applications: BA
POMC Antibody
NB100-1533
Flow Cytometry POMC Antibody
Immunohistochemistry POMC Antibody
Immunohistochemistry POMC Antibody
(10 Publications)
Species: Hu, Mu, Po, Rt, Sh
Applications: Flow, ICC/IF, IHC, IHC-P, PEP-ELISA, WB
DAP5 Antibody
NBP1-85311
Western Blot DAP5 Antibody
Western Blot DAP5 Antibody
Immunohistochemistry-Paraffin DAP5 Antibody
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, KD, WB
Gemin 4 Antibody
NB110-40591
Western Blot Gemin 4 Antibody
Immunohistochemistry-Paraffin Gemin 4 Antibody
(1 Publication)
Species: Hu
Applications: ICC/IF, IHC, IHC-P, IP, WB
Craniofacial
Development Protein 1 ...
NBP2-43772
Immunohistochemistry-Paraffin Craniofacial Development Protein 1 Antibody (11811)
Immunohistochemistry-Paraffin Craniofacial Development Protein 1 Antibody (11811)
Immunocytochemistry/ Immunofluorescence Craniofacial Development Protein 1 Antibody (11811)
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
Proteasome 19S 10B
Antibody
NB100-1483
Western Blot Proteasome 19S 10B Antibody
Immunocytochemistry/ Immunofluorescence Proteasome 19S 10B Antibody
Flow Cytometry Proteasome 19S 10B Antibody
(6 Publications)
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, PEP-ELISA, PLA, WB
C-Reactive
Protein/CRP [Biotin]
DY1707
N/A C-Reactive Protein/CRP [Biotin]
(68 Publications)
Species: Hu
Applications: ELISA
Teashirt homolog 1
Antibody
NBP1-87377
Immunohistochemistry-Paraffin Teashirt homolog 1 Antibody
Western Blot Teashirt homolog 1 Antibody
Immunocytochemistry/ Immunofluorescence Teashirt homolog 1 Antibody
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
LYAG/GAA Antibody
NBP2-38142
Western Blot LYAG/GAA Antibody
Immunohistochemistry-Paraffin LYAG/GAA Antibody
Immunohistochemistry-Paraffin LYAG/GAA Antibody
Species: Hu
Applications: IHC, IHC-P, WB
AGA Antibody
NBP1-88866
Immunohistochemistry-Paraffin AGA Antibody
Immunohistochemistry-Paraffin AGA Antibody
Immunohistochemistry-Paraffin AGA Antibody
(1 Publication)
Species: Hu
Applications: IHC, IHC-P
AAAS
Overexpression Lysate
NBL1-07159
AAAS Overexpression Lysate
Species: Hu
Applications: WB

Publications for AAAS Lysate (NBL1-07159) (0)

There are no publications for AAAS Lysate (NBL1-07159).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for AAAS Lysate (NBL1-07159) (0)

There are no reviews for AAAS Lysate (NBL1-07159). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for AAAS Lysate (NBL1-07159) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional AAAS Products

Blogs on AAAS

There are no specific blogs for AAAS, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
TNF-alpha [Unconjugated]
210-TA-005

Contact Information

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our AAAS Overexpression Lysate and receive a gift card or discount.


Bioinformatics

Gene Symbol AAAS