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14-3-3 zeta Antibody

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Western Blot: 14-3-3 zeta Antibody [NBP2-24593] - analysis of 14-3-3 zeta in human brain lysate in the 1) absence, 2) presence of immunizing peptide, 3) mouse brain lysate and 4) rat brain lysate in the absence of ...read more
Immunohistochemistry-Paraffin: 14-3-3 zeta Antibody [NBP2-24593] - Formalin-fixed, paraffin-embedded human spleen stained with 14-3-3 zeta antibody at 10 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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14-3-3 zeta Antibody Summary

Immunogen
A portion of amino acids 200-245 of human 14-3-3 zeta was used as the immunogen for the antibody.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
YWHAZ
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 10 ug/ml
  • Western Blot 1-3 ug/ml
Publications
Read Publication using
NBP2-24593 in the following applications:

  • WB
    1 publication

Reactivity Notes

The immunogen shows 100% sequence identity in human, cattle, rat, mouse, chimpanzee, rhesus monkey, opossum, pig, chicken and dog, and 94% in frog.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.2 ml PBS and 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A purified

Alternate Names for 14-3-3 zeta Antibody

  • 1433 zeta
  • 14-3-3 zeta
  • KCIP-1
  • KCIP-1MGC126532,14-3-3-zeta
  • MGC111427
  • MGC138156
  • tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, deltapolypeptide
  • tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zetapolypeptide
  • YWHAZ
  • zeta polypeptide

Background

14-3-3 zeta is a protein that mediates signal transduction by binding to phosphoserine-containing proteins. The protein is widely expressed in various gray matter regions of the brain. Overexpression of the 14-3-3 zeta gene has been found to correlate with chemoresistance to anthracyclines and metastatis recurrence of human breast cancer. These effects are likely caused by an anti-apoptotic mechanism of 14-3-3 zeta during anthracycline treatment.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for 14-3-3 zeta Antibody (NBP2-24593)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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WB
(1)
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Human
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Showing Publication 1 - 1 of 1.
Publication using NBP2-24593 Applications Species
Fehilly J, Carey O, O�Leary E et al. Condensate formation of the human RNA-binding protein SMAUG1 is controlled by its intrinsically disordered regions and interactions with 14-3-3 proteins bioRxiv 2023-02-10 (WB, Human) WB Human

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol YWHAZ