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14-3-3 zeta Antibody

Images

 
Western Blot: 14-3-3 zeta Antibody [NBP1-31325] - Sample(30 ug of whole cell lysate) A:Hep G2 B:MOLT4 12% SDS PAGE, antibody diluted at 1:2000.
Immunocytochemistry/ Immunofluorescence: 14-3-3 zeta Antibody [NBP1-31325] - Analysis of HeLaS3, using 14-3-3 zeta/delta antibody at 1:100 dilution.
Immunohistochemistry-Paraffin: 14-3-3 zeta Antibody [NBP1-31325] - Mouse colon. 14-3-3 zeta antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Western Blot: 14-3-3 zeta Antibody [NBP1-31325] - Sample (30 ug of whole cell lysate) A:NIH-3T3 12% SDS PAGE; antibody diluted at 1:1000.
Immunohistochemistry-Paraffin: 14-3-3 zeta Antibody [NBP1-31325] - Cal27 xenograft, using 14-3-3 zeta antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Western Blot: 14-3-3 zeta Antibody [NBP1-31325] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with 14-3-3 zeta antibody (NBP1-31325) diluted at 1:2000. The ...read more
Western Blot: 14-3-3 zeta Antibody [NBP1-31325] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with 14-3-3 zeta antibody (NBP1-31325) diluted at 1:1000. The ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Rt, Po, Bv, Ch, RM, ShSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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14-3-3 zeta Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human 14-3-3 zeta. The exact sequence is proprietary.
Localization
Cytoplasm, Melanosome
Predicted Species
Rat (99%), Porcine (99%), Bovine (100%), Rhesus Macaque (100%), Chicken (99%), Sheep (99%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
YWHAZ
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
28 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using NBP1-31325.

Reactivity Notes

Xenopus laevis (89%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
Preservative
0.025% Proclin 300
Purity
Antigen Affinity-purified

Alternate Names for 14-3-3 zeta Antibody

  • 1433 zeta
  • 14-3-3 zeta
  • KCIP-1
  • KCIP-1MGC126532,14-3-3-zeta
  • MGC111427
  • MGC138156
  • tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, deltapolypeptide
  • tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zetapolypeptide
  • YWHAZ
  • zeta polypeptide

Background

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Two transcript variants differing in the 5' UTR, but encoding the same protein, have been identified for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for 14-3-3 zeta Antibody (NBP1-31325)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for 14-3-3 zeta Antibody (NBP1-31325) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol YWHAZ