![Western Blot: 14-3-3 sigma/Stratifin Antibody [NBP2-98309] - Anti-14-3-3 sigma/Stratifin rabbit polyclonal antibody at 1:500 dilution. Lane A: NIH3T3 Whole Cell Lysate. Lane B: Raw 264.7 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 27 kDa](http://images.novusbio.com/fullsize/14-3-3-sigma-Stratifin-Antibody-Western-Blot-NBP2-98309-img0006.jpg "Western Blot: 14-3-3 sigma/Stratifin Antibody [NBP2-98309] - Anti-14-3-3 sigma/Stratifin rabbit polyclonal antibody at 1:500 dilution. Lane A: NIH3T3 Whole Cell Lysate. Lane B: Raw 264.7 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 27 kDa")
| Reactivity | MuSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IHC, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Description | This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles. |
| Immunogen | Produced in rabbits immunized with purified, recombinant Mouse 14-3-3 sigma/Stratifin (Accession#: NP_061224.2; Met1-Ser248). |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | SFN |
| Purity | Antigen and protein A Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 0.2 um filtered solution in PBS |
| Preservative | No Preservative |
| Purity | Antigen and protein A Affinity-purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for 14-3-3 sigma/Stratifin Antibody (NBP2-98309)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | SFN |
![Immunocytochemistry/Immunofluorescence: 14-3-3 sigma/Stratifin Antibody [NBP2-98309] - Immunofluorescence staining of 14-3-3 sigma/Stratifin in Raw264.7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Mouse 14-3-3 sigma/Stratifin polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green). Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/14-3-3-sigma-Stratifin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-98309-img0001.jpg "Immunocytochemistry/Immunofluorescence: 14-3-3 sigma/Stratifin Antibody [NBP2-98309] - Immunofluorescence staining of 14-3-3 sigma/Stratifin in Raw264.7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Mouse 14-3-3 sigma/Stratifin polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green). Positive staining was localized to Cytoplasm.")
![Immunohistochemistry-Paraffin: 14-3-3 sigma/Stratifin Antibody [NBP2-98309] - Immunochemical staining of mouse 14-3-3 sigma/Stratifin in mouse skin with rabbit polyclonal antibody at 1:20000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/14-3-3-sigma-Stratifin-Antibody-Immunohistochemistry-Paraffin-NBP2-98309-img0004.jpg "Immunohistochemistry-Paraffin: 14-3-3 sigma/Stratifin Antibody [NBP2-98309] - Immunochemical staining of mouse 14-3-3 sigma/Stratifin in mouse skin with rabbit polyclonal antibody at 1:20000 dilution, formalin-fixed paraffin embedded sections.")
![Immunoprecipitation: 14-3-3 sigma/Stratifin Antibody [NBP2-98309] - Mouse 14-3-3 sigma/Stratifin was immunoprecipitated using: Lane A: 0.5 mg NIH3T3 Whole Cell Lysate. 4 ul anti-Mouse 14-3-3 sigma/Stratifin rabbit polyclonal antibody and 60 ug of Immunomagnetic beads. Protein A/G. Primary antibody: Anti-Mouse 14-3-3 sigma/Stratifin rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 30 kDa. Observed band size: 30 kDa](http://images.novusbio.com/fullsize/14-3-3-sigma-Stratifin-Antibody-Immunoprecipitation-NBP2-98309-img0005.jpg "Immunoprecipitation: 14-3-3 sigma/Stratifin Antibody [NBP2-98309] - Mouse 14-3-3 sigma/Stratifin was immunoprecipitated using: Lane A: 0.5 mg NIH3T3 Whole Cell Lysate. 4 ul anti-Mouse 14-3-3 sigma/Stratifin rabbit polyclonal antibody and 60 ug of Immunomagnetic beads. Protein A/G. Primary antibody: Anti-Mouse 14-3-3 sigma/Stratifin rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 30 kDa. Observed band size: 30 kDa")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")