![Western Blot: RB1 Antibody [NBP2-20127] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Rb antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/RB1-Antibody-Western-Blot-NBP2-20127-img0008.jpg "Western Blot: RB1 Antibody [NBP2-20127] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Rb antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
| Reactivity | Hu, Mu, Rt, Po, Bv, RMSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human RB1. The exact sequence is proprietary. |
| Localization | Nucleus |
| Predicted Species | Rat (96%), Porcine (96%), Bovine (97%), Rhesus Macaque (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | RB1 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Theoretical MW | 106 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7), 20% Glycerol |
| Preservative | 0.025% Proclin 300 |
| Purity | Antigen Affinity-purified |
![Western Blot PAK3 Antibody [Unconjugated]](https://images.novusbio.com/images2/PAK3_AF6897_Western_Blot_11082.jpg)
![Immunohistochemistry PAK3 Antibody [Unconjugated]](https://images.novusbio.com/images2/PAK3_AF6897_Immunohistochemistry_11144.jpg)
![Immunohistochemistry PAK3 Antibody [Unconjugated]](https://images.novusbio.com/images2/PAK3_AF6897_Immunohistochemistry_11480.jpg)
| Publication using NBP2-20127 | Applications | Species |
|---|---|---|
| Weber M, Nguyen MB, Li MY et al. Merkel cell polyomavirus T-antigen-mediated reprogramming in adult Merkel cell progenitors The Journal of investigative dermatology 2023-05-29 [PMID: 37257637] (IHC, Mouse) | IHC | Mouse |
Secondary Antibodies |
Isotype Controls |
Research Areas for RB1 Antibody (NBP2-20127)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | RB1 |
![Immunocytochemistry/Immunofluorescence: RB1 Antibody [NBP2-20127] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Rb stained by Rb antibody diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar= 10 um.](http://images.novusbio.com/fullsize/RB1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20127-img0007.jpg "Immunocytochemistry/Immunofluorescence: RB1 Antibody [NBP2-20127] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Rb stained by Rb antibody diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar= 10 um.")
![Immunohistochemistry-Paraffin: RB1 Antibody [NBP2-20127] - Mouse eye. Green: Rb stained by Rb antibody diluted at 1:500.Red: beta Tubulin 3/ Tuj1, a cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [11710] diluted at 1:500. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/RB1-Antibody-Immunohistochemistry-Paraffin-NBP2-20127-img0006.jpg "Immunohistochemistry-Paraffin: RB1 Antibody [NBP2-20127] - Mouse eye. Green: Rb stained by Rb antibody diluted at 1:500.Red: beta Tubulin 3/ Tuj1, a cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [11710] diluted at 1:500. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Western Blot: RB1 Antibody [NBP2-20127] - Rb antibody detects Rb1 protein by western blot analysis. A. 30 ug A431 whole cell lysate/extract 5% SDS-PAGE Rb1 antibody dilution: 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/RB1-Antibody-Western-Blot-NBP2-20127-img0002.jpg "Western Blot: RB1 Antibody [NBP2-20127] - Rb antibody detects Rb1 protein by western blot analysis. A. 30 ug A431 whole cell lysate/extract 5% SDS-PAGE Rb1 antibody dilution: 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: RB1 Antibody [NBP2-20127] - Human colon carcinoma. Rb antibody dilution: 1:500. Antigen Retrieval: TrilogyTM (EDTA based) buffer, 15min](http://images.novusbio.com/fullsize/RB1-Antibody-Immunohistochemistry-Paraffin-NBP2-20127-img0005.jpg "Immunohistochemistry-Paraffin: RB1 Antibody [NBP2-20127] - Human colon carcinoma. Rb antibody dilution: 1:500. Antigen Retrieval: TrilogyTM (EDTA based) buffer, 15min")
![Immunoprecipitation: RB1 Antibody [NBP2-20127] - HeLa whole cell extract A. Control with 4 ug of preimmune Rabbit IgG B. Immunoprecipitation of retinoblastoma 1 protein by 4 ug Rb1 antibody 7.5 % SDS-PAGE The immunoprecipitated retinoblastoma 1 protein was detected by Rb1 antibody diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]](http://images.novusbio.com/fullsize/RB1-Antibody-Immunoprecipitation-NBP2-20127-img0004.jpg "Immunoprecipitation: RB1 Antibody [NBP2-20127] - HeLa whole cell extract A. Control with 4 ug of preimmune Rabbit IgG B. Immunoprecipitation of retinoblastoma 1 protein by 4 ug Rb1 antibody 7.5 % SDS-PAGE The immunoprecipitated retinoblastoma 1 protein was detected by Rb1 antibody diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]")
![Western Blot p27/Kip1 [p Thr157] Antibody](https://images.novusbio.com/images2/p27_AF1555_Western_Blot_5119.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")