![Western Blot: RB1 Antibody (S09-4H8) [NBP3-19971] - Western blot detection of RB1 in 3T3 cell lysates using NBP3-19971 (1:1000 diluted). Predicted band size: 106kDa. Observed band size: 110kDa.](http://images.novusbio.com/fullsize/RB1-Antibody-S09-4H8-Western-Blot-NBP3-19971-img0002.jpg "Western Blot: RB1 Antibody (S09-4H8) [NBP3-19971] - Western blot detection of RB1 in 3T3 cell lysates using NBP3-19971 (1:1000 diluted). Predicted band size: 106kDa. Observed band size: 110kDa.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ICC/IF |
| Clone | S09-4H8 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 0.3 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | A synthetic peptide of human RB1 (Uniprot # P06400) |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | RB1 |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 110 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA |
| Preservative | 0.01% Sodium Azide |
| Concentration | 0.3 mg/ml |
| Purity | Affinity purified |
![Western Blot PAK3 Antibody [Unconjugated]](https://images.novusbio.com/images2/PAK3_AF6897_Western_Blot_11082.jpg)
![Immunohistochemistry PAK3 Antibody [Unconjugated]](https://images.novusbio.com/images2/PAK3_AF6897_Immunohistochemistry_11144.jpg)
![Immunohistochemistry PAK3 Antibody [Unconjugated]](https://images.novusbio.com/images2/PAK3_AF6897_Immunohistochemistry_11480.jpg)
Secondary Antibodies |
Isotype Controls |
Research Areas for RB1 Antibody (NBP3-19971)Find related products by research area.
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| Gene Symbol | RB1 |
![Immunocytochemistry/Immunofluorescence: RB1 Antibody (S09-4H8) [NBP3-19971] - Immunocytochemistry of RB1 (green) in Hela cells using NBP3-19971 at dilution 1/200, and DAPI (blue)](http://images.novusbio.com/fullsize/RB1-Antibody-S09-4H8-Immunocytochemistry-Immunofluorescence-NBP3-19971-img0001.jpg "Immunocytochemistry/Immunofluorescence: RB1 Antibody (S09-4H8) [NBP3-19971] - Immunocytochemistry of RB1 (green) in Hela cells using NBP3-19971 at dilution 1/200, and DAPI (blue)")
![Western Blot p27/Kip1 [p Thr157] Antibody](https://images.novusbio.com/images2/p27_AF1555_Western_Blot_5119.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")