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MMR/CD206/Mannose Receptor Antibody [Unconjugated]

Images

 
Western blot shows lysates of mouse liver tissue. Gels were loaded with 12 µg, 6.5 µg, and 3 µg of tissue lysate. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse MMR/CD206 Antigen Affinity-purified ...read more
MMR/CD206 was detected in perfusion fixed frozen sections of mouse testis using Goat Anti-Mouse MMR/CD206 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2535) at 5 µg/mL overnight at 4 °C. Tissue was ...read more
MMR/CD206 was detected in perfusion fixed frozen sections of mouse lung using Goat Anti-Mouse MMR/CD206 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2535) at 25 µg/mL overnight at 4 °C. Tissue was ...read more
Cells of human meninges co-express LLEC markers. a–c DAB-IHC with single antibodies detects VEGFR3 (a), LYVE1 (b), and MRC1 (c) in the meninges of human post mortem brain showing no signs of neuropathology. These ...read more
M1 and M2 phenotype in spinal cord after intraplantar IL-1 beta . Wild-type (WT) and LysM-G protein–coupled receptor kinase (GRK)2+/− mice received an intraplantar injection of 1 ng IL-1 beta . At 15 hours after ...read more
Immunofluorescent staining for macrophage marker F4/80 in Acomys and Mus.(A–C) Bone-marrow-derived cells isolated from Acomys and stained for F4/80 (green). (A) unstimulated cells, (B) cells stimulated with IFN gamma ...read more
Characterization of M2 BV2 cells induced by IL-4 and identification of sEVs derived from M2 BV2 cells. (A, B) Representative images of BV2 cells immunostained for Iba-1 (green), CD206 (red), and arginase (red). ...read more
Cells of human meninges co-express LLEC markers. a–c DAB-IHC with single antibodies detects VEGFR3 (a), LYVE1 (b), and MRC1 (c) in the meninges of human post mortem brain showing no signs of neuropathology. These ...read more
Mouse LLECs take up A beta 1-40. a Schematic showing the site of dye and A beta 1-40 perfusion into the CSF via the cisterna magna (arrow) of a 2-month old mouse. The dotted line indicates the plane of section. A ...read more
Cells of human meninges co-express LLEC markers. a–c DAB-IHC with single antibodies detects VEGFR3 (a), LYVE1 (b), and MRC1 (c) in the meninges of human post mortem brain showing no signs of neuropathology. These ...read more
Immunofluorescent staining for macrophage marker F4/80 in Acomys and Mus.(A–C) Bone-marrow-derived cells isolated from Acomys and stained for F4/80 (green). (A) unstimulated cells, (B) cells stimulated with IFN gamma ...read more
Mouse LLECs take up A beta 1-40. a Schematic showing the site of dye and A beta 1-40 perfusion into the CSF via the cisterna magna (arrow) of a 2-month old mouse. The dotted line indicates the plane of section. A ...read more
In vitro activation assays shows Acomys macrophages can be polarized to express different markers.(A–I) Bone-marrow-derived macrophages isolated from Acomys femurs are cultured with no cytokines (unstimulated, A, D, ...read more
Microglial activation was attenuated by Hv1 deletion following LPC-induced demyelination. a Representative images of Iba-1 immunostaining in the CC of WT and Hv1−/− mice (scale bar, 200 μm). b Quantification of ...read more
Cells with BLEC molecular markers are present within the mouse leptomeninges. a Coronal brain section of adult zebrafish brain indicating the imaging area in the dorsal optic tectum (TeO). b A 14 month old ...read more
M1 and M2 phenotype in spinal cord after intraplantar IL-1 beta . Wild-type (WT) and LysM-G protein–coupled receptor kinase (GRK)2+/− mice received an intraplantar injection of 1 ng IL-1 beta . At 15 hours after ...read more
Cells of human meninges co-express LLEC markers. a–c DAB-IHC with single antibodies detects VEGFR3 (a), LYVE1 (b), and MRC1 (c) in the meninges of human post mortem brain showing no signs of neuropathology. These ...read more
Representative images of HE, M3/84 (macrophages) and MR (CD206) immunohistochemistry. In control mice, no plaques with macrophages were observed, while fibrous/fibroatheromatous plaques were present in the aortas ...read more
TAMs along the beam paths show a mixed M2-like/M1-like phenotype and expression of phagocytosis markers. (A) Double staining for CD68 (red) and CD206 (green) plus DAPI (blue) shows at 7 days post-MRT that many of the ...read more
Hypothermia promotes a shift of microglia/macrophages towards an anti‐inflammatory phenotype in aged female mice 7 days after ischemic stroke. Mice were subjected to distal MCAO (dMCAO) or sham surgery. Normothermia ...read more
HCK knockout reduced kidney fibrosis in uIRIx model with regulation of macrophage activation.A Schematic diagram of strategy of uIRIx models. B Urine ACR and serum BUN for WT and HCK KO uIRIx mice. C Representative ...read more
Hypothermia promotes a shift of microglia/macrophages towards an anti‐inflammatory phenotype in aged female mice 7 days after ischemic stroke. Mice were subjected to distal MCAO (dMCAO) or sham surgery. Normothermia ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Flow, IHC, CyTOF-ready
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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MMR/CD206/Mannose Receptor Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse MMR/CD206
Leu19-Ala1388
Accession # Q2HZ94
Specificity
Detects mouse MMR/CD206 in direct ELISAs and Western blots. In direct ELISAs, less than 45% cross-reactivity with recombinant human MMR is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
MRC1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL
Reviewed Applications
Read 3 Reviews rated 4.3
using
AF2535 in the following applications:

Publications
Read Publications using
AF2535 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MMR/CD206/Mannose Receptor Antibody [Unconjugated]

  • CD206
  • CLEC13D
  • CLEC13Dmacrophage mannose receptor 1
  • C-type lectin domain family 13 member D
  • mannose receptor, C type 1
  • MMR
  • MMRCD206 antigen
  • MRC1

Background

The mouse Macrophage Mannose Receptor (MMR), also known as CD206 and MRC1 (mannose receptor C, type 1), is a 175 kDa scavenger receptor that is expressed on tissue macrophages, myeloid dendritic cells, and liver and lymphatic endothelial cells (1). It belongs to a family of receptors sharing similar protein structure that also includes DEC205, phospholipase A2 receptor, and Endo180 (2, 3). The mouse MMR protein is synthesized as a 1456 amino acid (aa) precursor that contains a 19 aa signal sequence, a 1369 aa extracellular region, a 21 aa transmembrane segment and a 47 aa cytoplasmic domain (4). Its extracellular region is composed of an N-terminal cysteine-rich domain, followed by a single fibronectin type II repeat, and eight C-type lectin carbohydrate recognition domains (CRD) (3‑5). Mouse to human, the extracellular region is 82% aa identical. The cysteine-rich domain mediates recognition of sulfated N-acetylgalactosamine, which occurs on some extracellular matrix proteins and is the terminal sugar of the unusual oligosaccharides present on pituitary hormones such as lutropin and thyrotropin (6). Several of the CRDs participate in the Ca2+-dependent recognition of carbohydrates showing a preference for branched sugars with terminal mannose, fucose or N‑acetylglucosamine (7). The cytoplasmic domain of MMR includes a tyrosine-based motif for internalization in clathrin-coated vesicles. Once internalized, ligands are released following acidification of phagosomes or endosomes, and the receptor recycles to the cell surface (3, 8). MMR mediates phagocytosis upon binding to target structures that occur on a variety of pathogenic microorganisms including Gram-negative and Gram-positive bacteria, yeasts, parasites, and mycobacteria. MMR also functions to maintain homeostasis through the endocytosis of potentially harmful glycoproteins associated with inflammation (2, 3).

  1. East, L. and C. Isake (2002) Biochim. Biophys. Acta 1572:364. 
  2. Chieppa, M. et al. (2003) J. Immunol. 171:4552. 
  3. Figdor, C. et al. (2002) Nat. Rev. Immunol. 2:77. 
  4. Harris, N. et al. (1992) Blood 80:2363.
  5. Taylor, M. et al. (1990) J. Biol. Chem. 265:12156.
  6. Leteux, C. et al. (2000) J. Exp. Med. 191:1117.
  7. Martinez-Pomares, L. et al. (2001) Immunobiology 204:527.
  8. Feinberg, H. et al. (2000) J. Biol. Chem. 275:21539.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MMR/CD206/Mannose Receptor Antibody (AF2535)(193)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Transgenic Mouse.

We have publications tested in 12 applications: Flow Cytometry, ICC, IF, IHC, IHC-Fr, IHC-P, IHC-Pr, IHC/IF, Immunocytochemistry, Immunohistochemistry, Neutralization, Western Blot.


Filter By Application
Flow Cytometry
(5)
ICC
(12)
IF
(4)
IHC
(56)
IHC-Fr
(11)
IHC-P
(3)
IHC-Pr
(1)
IHC/IF
(2)
Immunocytochemistry
(1)
Immunohistochemistry
(6)
Neutralization
(1)
Western Blot
(12)
All Applications
Filter By Species
Human
(3)
Mouse
(90)
Rat
(4)
Transgenic Mouse
(3)
All Species
Showing Publications 1 - 10 of 193. Show All 193 Publications.
Publications using AF2535 Applications Species
Tanaka, T;Isonishi, A;Banja, M;Yamamoto, R;Sonobe, M;Okuda-Ashitaka, E;Furue, H;Okuda, H;Tatsumi, K;Wanaka, A; Dermal macrophages control tactile perception under physiological conditions via NGF signaling Scientific reports 2024-11-08 [PMID: 39516548] (Western Blot, Transgenic Mouse) Western Blot Transgenic Mouse
Liao, R;Dewey, MJ;Rong, J;Johnson, SA;D'Angelo, WA;Hussey, GS;Badylak, SF; Matrix-bound nanovesicles alleviate particulate-induced periprosthetic osteolysis Science advances 2024-10-18 [PMID: 39423278] (Immunohistochemistry, Mouse) Immunohistochemistry Mouse
Korte, N;Barkaway, A;Wells, J;Freitas, F;Sethi, H;Andrews, SP;Skidmore, J;Stevens, B;Attwell, D; Inhibiting Ca2+ channels in Alzheimer's disease model mice relaxes pericytes, improves cerebral blood flow and reduces immune cell stalling and hypoxia Nature neuroscience 2024-09-18 [PMID: 39294491] (Immunohistochemistry, Mouse) Immunohistochemistry Mouse
Chen, D;Zhou, Z;Kong, N;Xu, T;Liang, J;Xu, P;Yao, B;Zhang, Y;Sun, Y;Li, Y;Wu, B;Yang, X;Wang, H; Inhalable SPRAY nanoparticles by modular peptide assemblies reverse alveolar inflammation in lethal Gram-negative bacteria infection Science advances 2024-09-13 [PMID: 39270015] (Immunohistochemistry, Immunocytochemistry, Mouse) Immunohistochemistry, Immunocytochemistry Mouse
Sutthiwanjampa, C;Kang, SH;Kim, MK;Hwa Choi, J;Kim, HK;Woo, SH;Bae, TH;Kim, WJ;Kang, SH;Park, H; Tumor necrosis factor-?-treated human adipose-derived stem cells enhance inherent radiation tolerance and alleviate in vivo radiation-induced capsular contracture Journal of advanced research 2024-07-15 [PMID: 39019109] (IHC-Pr, Human) IHC-Pr Human
Su, Q;Su, C;Zhang, Y;Guo, Y;Liu, Y;Liu, Y;Yong, VW;Xue, M; Adjudin protects blood-brain barrier integrity and attenuates neuroinflammation following intracerebral hemorrhage in mice International immunopharmacology 2024-04-01 [PMID: 38565042] (Immunohistochemistry, Mouse) Immunohistochemistry Mouse
Mader, MM;Napole, A;Wu, D;Atkins, M;Scavetti, A;Shibuya, Y;Foltz, A;Hahn, O;Yoo, Y;Danziger, R;Tan, C;Wyss-Coray, T;Steinman, L;Wernig, M; Myeloid cell replacement is neuroprotective in chronic experimental autoimmune encephalomyelitis Nature neuroscience 2024-03-21 [PMID: 38514857] (IHC/IF, Mouse) IHC/IF Mouse
Del Pilar, C;Garrido-Matilla, L;Del Pozo-Filíu, L;Lebrón-Galán, R;Arias, RF;Clemente, D;Alonso, JR;Weruaga, E;Díaz, D; Intracerebellar injection of monocytic immature myeloid cells prevents the adverse effects caused by stereotactic surgery in a model of cerebellar neurodegeneration Journal of neuroinflammation 2024-02-14 [PMID: 38355633] (IHC, Mouse) IHC Mouse
Surve, CR;Duran, CL;Ye, X;Chen, X;Lin, Y;Harney, AS;Wang, Y;Sharma, VP;Stanley, ER;McAuliffe, JC;Entenberg, D;Oktay, MH;Condeelis, JS; Signaling events at TMEM doorways provide potential targets for inhibiting breast cancer dissemination bioRxiv : the preprint server for biology 2024-01-09 [PMID: 38260319] (IHC, Mouse) IHC Mouse
Vlk, AM;Prantner, D;Shirey, KA;Perkins, DJ;Buzza, MS;Thumbigere-Math, V;Keegan, AD;Vogel, SN; M2a macrophages facilitate resolution of chemically-induced colitis in TLR4-SNP mice mBio 2023-09-28 [PMID: 37768050] (Western Blot, Mouse) Western Blot Mouse
Show All 193 Publications.

Reviews for MMR/CD206/Mannose Receptor Antibody (AF2535) (3) 4.33

Average Rating: 4.3
(Based on 3 reviews)
We have 3 reviews tested in 2 species: Mouse, Mouse and Rat.

Reviews using AF2535:
Filter by Applications
IHC-Fr
(2)
IHC-P
(1)
All Applications
Filter by Species
Mouse
(2)
Mouse and Rat
(1)
All Species
Images Ratings Applications Species Date Details
  5
reviewed by:
Verified Customer
IHC-Fr Mouse 01/18/2023
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedMouse Spleen
SpeciesMouse
LotWFT0322071
Immunohistochemistry-Paraffin MMR/CD206/Mannose Receptor AF2535
Enlarge
4
reviewed by:
Imke Jansen
IHC-P Mouse and Rat 03/16/2018
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedTestis
SpeciesMouse and Rat
LotWFT0118011

Comments

CommentsMouse and Rat Testis: 4% Neutral buffered Formalin fixated for 3 days and subsequently dehydrated in alcohol steps (2x 70%, 2x 96%, 2x 100%) xyleen and paraffin embedded.

Primary antibodies:
1/100 dilution     MMR/CD206 Goat polyclonal IgG      AF2535
Lot#WFT0118011
1/500 dilution     Goat IgG isotype 10 ug/ml     02-6202
Lot#TA262200
both working concentration: 10 ug/mL

Since rat species have not been tested for MMR/CD206 antibody (AF2535), we decided to take part in the Innovator’s Reward™ program.
MMR/CD206 was already detected in perfusion fixed frozen sections of mouse testis with specific staining localized to spermatocytes in testis. Therefore, we included mouse testis (positive control) and rat testis tissue, that is formalin fixated and paraffin embedded.
  4
reviewed by:
Verified Customer
IHC-Fr Mouse 01/07/2015
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedSee PMID 23942366
SpeciesMouse

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Recent Reviews

4.3
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2
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Verified Customer
01/18/2023
Application: IHC-Fr
Species: Mouse

Imke Jansen
03/16/2018
Application: IHC-P
Species: Mouse and Rat

 
Verified Customer
01/07/2015
Application: IHC-Fr
Species: Mouse

Bioinformatics

Gene Symbol MRC1
Uniprot