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![Western Blot: p97/VCP Antibody [NB100-1558] - Zymographic assay of gelatinase activity. Medium supernatants of 20ml culture medium (medium control) as well as 20 ml conditioned medium from 107 U937 cells, pMTH1-U937 cells and asCD11b-U937 cells in the absence or presence of 5nM TPA for 72 h, respectively, were 18-fold concentrated and subjected to SDS-PAGE containing 2 mg/ml of gelatine. Following incubation with MMP enzyme buffer, the gels were stained with 0.4% Coomassie blue and afterwards destained again to visualize the appearance of gelatinase activity by light bands against the dark background. The molecular weight markers on both sides of the gels indicate the size of the MMPs exhibiting gelatinase activities. Image collected and cropped by CiteAb from the following publication (//biosignaling.biomedcentral.com/articles/10.1186/1478-811X-10-13) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/p97-VCP-Antibody-Western-Blot-NB100-1558-img0015.jpg "Western Blot: p97/VCP Antibody [NB100-1558] - Zymographic assay of gelatinase activity. Medium supernatants of 20ml culture medium (medium control) as well as 20 ml conditioned medium from 107 U937 cells, pMTH1-U937 cells and asCD11b-U937 cells in the absence or presence of 5nM TPA for 72 h, respectively, were 18-fold concentrated and subjected to SDS-PAGE containing 2 mg/ml of gelatine. Following incubation with MMP enzyme buffer, the gels were stained with 0.4% Coomassie blue and afterwards destained again to visualize the appearance of gelatinase activity by light bands against the dark background. The molecular weight markers on both sides of the gels indicate the size of the MMPs exhibiting gelatinase activities. Image collected and cropped by CiteAb from the following publication (//biosignaling.biomedcentral.com/articles/10.1186/1478-811X-10-13) licensed under a CC-BY license.")
![Western Blot: p97/VCP Antibody [NB100-1558] - Whole cell lysate (15 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit antiVCP antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.](http://images.novusbio.com/fullsize/p97-VCP-Antibody-Western-Blot-NB100-1558-img0012.jpg)
![Western Blot: p97/VCP Antibody [NB100-1558] - Detection of human VCP by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-VCP antibody NB100-1558 used for IP at 3 ug per reaction. VCP was also immunoprecipitated by rabbit anti-VCP antibody NB100-1557. For blotting immunoprecipitated VCP, NB100-1558 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.](http://images.novusbio.com/fullsize/p97-VCP-Antibody-Western-Blot-NB100-1558-img0013.jpg)
![Western Blot: p97/VCP Antibody [NB100-1558] - Zymographic assay of gelatinase activity. Medium supernatants of 20 ml culture medium (medium control) as well as 20 ml conditioned medium from 107 U937 cells, pMTH1-U937 cells & asCD11b-U937 cells in the absence or presence of 5nM TPA for 72 h, respectively, were 18-fold concentrated & subjected to SDS-PAGE containing 2 mg/ml of gelatine. Following incubation with MMP enzyme buffer, the gels were stained with 0.4% Coomassie blue & afterwards destained again to visualize the appearance of gelatinase activity by light bands against the dark background. The molecular weight markers on both sides of the gels indicate the size of the MMPs exhibiting gelatinase activities. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/22607136), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1558_rabbit-polyclonal-p97-vcp-antibody-31020241535618.jpg)
![Western Blot: p97/VCP Antibody [NB100-1557] - PC12 whole cell lysate, 30 ug total protein in each lane. Antibody at 1:1000. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/p97-VCP-Antibody-Western-Blot-NB100-1557-img0008.jpg "Western Blot: p97/VCP Antibody [NB100-1557] - PC12 whole cell lysate, 30 ug total protein in each lane. Antibody at 1:1000. Image submitted by a verified customer review.")
![Immunohistochemistry-Paraffin: p97/VCP Antibody [NB100-1557] - Sections of human ovarian carcinoma (upper) and mouse squamous cell carcinoma (lower). Antibody: Affinity purified rabbit anti-VCP used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/p97-VCP-Antibody-Immunohistochemistry-NB100-1557-img0007.jpg)
![p97/VCP Antibody [NB100-1557] - Detection of human and mouse VCP by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-VCP antibody NB100-1557 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/p97-VCP-Antibody-NB100-1557-img0009.jpg)
![p97/VCP Antibody [NB100-1557] - Detection of human VCP by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-VCP antibody NB100-1557 used for IP at 3 ug per reaction. VCP was also immunoprecipitated by rabbit anti-VCP antibody NB100-1558. For blotting immunoprecipitated VCP, NB100-1557 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/p97-VCP-Antibody-NB100-1557-img0010.jpg)
![Western Blot: p97/VCP Antibody (4A8) [H00007415-M03] - Analysis of VCP expression in Hela S3 NE.](http://images.novusbio.com/fullsize/p97-VCP-Antibody-4A8-Western-Blot-H00007415-M03-img0005.jpg "Western Blot: p97/VCP Antibody (4A8) [H00007415-M03] - Analysis of VCP expression in Hela S3 NE.")
![Immunohistochemistry-Paraffin: p97/VCP Antibody (4A8) [H00007415-M03] - Analysis of monoclonal antibody to VCP on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml](http://images.novusbio.com/fullsize/p97-VCP-Antibody-4A8-Immunohistochemistry-Paraffin-H00007415-M03-img0002.jpg)
![Western Blot: p97/VCP Antibody (4A8) [H00007415-M03] - VCP monoclonal antibody (M03), clone 4A8. Western Blot analysis of VCP expression in HepG2 ( Cat # L019V1 ).](http://images.novusbio.com/fullsize/p97-VCP-Antibody-4A8-Western-Blot-H00007415-M03-img0001.jpg)
![Western Blot: p97/VCP Antibody (4A8) [H00007415-M03] - Analysis of VCP expression in PC-12.](http://images.novusbio.com/fullsize/p97-VCP-Antibody-4A8-Western-Blot-H00007415-M03-img0004.jpg)
![Western Blot: p97/VCP Antibody (4A8) [H00007415-M03] - Analysis of VCP expression in Raw 264.7.](http://images.novusbio.com/fullsize/p97-VCP-Antibody-4A8-Western-Blot-H00007415-M03-img0006.jpg)
![ELISA: p97/VCP Antibody (4A8) [H00007415-M03] - Detection limit for recombinant GST tagged VCP is 1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/p97-VCP-Antibody-4A8-ELISA-H00007415-M03-img0007.jpg)
![Western Blot: p97/VCP Antibody [NBP1-81619] - Analysis in A-549 cells transfected with control siRNA, target specific siRNA probe #1. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/p97-VCP-Antibody-Western-Blot-NBP1-81619-img0008.jpg "<b>Genetic Strategies Validation. </b>Western Blot: p97/VCP Antibody [NBP1-81619] - Analysis in A-549 cells transfected with control siRNA, target specific siRNA probe #1. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: p97/VCP Antibody [NBP1-81619] - Staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.](http://images.novusbio.com/fullsize/p97-VCP-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-81619-img0006.jpg)
![Immunohistochemistry-Paraffin: p97/VCP Antibody [NBP1-81619] - Staining of human skeletal muscle shows moderate nuclear and cytoplasmic positivity in a subset of myocytes.](http://images.novusbio.com/fullsize/p97-VCP-Antibody-Immunohistochemistry-Paraffin-NBP1-81619-img0011.jpg)
![Immunohistochemistry-Paraffin: p97/VCP Antibody [NBP1-81619] - Staining of human prostate shows strong nuclear and cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/p97-VCP-Antibody-Immunohistochemistry-Paraffin-NBP1-81619-img0009.jpg)
![Immunohistochemistry-Paraffin: p97/VCP Antibody [NBP1-81619] - Staining of human endometrium shows strong nuclear and cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/p97-VCP-Antibody-Immunohistochemistry-Paraffin-NBP1-81619-img0010.jpg)
![Immunohistochemistry-Paraffin: p97/VCP Antibody [NBP1-81619] - Staining of human gastrointestinal shows strong nuclear and cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/p97-VCP-Antibody-Immunohistochemistry-Paraffin-NBP1-81619-img0012.jpg)
![Western Blot: p97/VCP Antibody [NBP1-81620] - Analysis in A-549 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-VCP antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/p97-VCP-Antibody-Western-Blot-NBP1-81620-img0011.jpg "<b>Genetic Strategies Validation. </b>Western Blot: p97/VCP Antibody [NBP1-81620] - Analysis in A-549 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-VCP antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: p97/VCP Antibody [NBP1-81620] - Staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.](http://images.novusbio.com/fullsize/p97-VCP-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-81620-img0008.jpg)
![Immunohistochemistry-Paraffin: p97/VCP Antibody [NBP1-81620] - Staining of human hippocampus shows strong nuclear and cytoplasmic positivity in neuronal cells and glial cells.](http://images.novusbio.com/fullsize/p97-VCP-Antibody-Immunohistochemistry-Paraffin-NBP1-81620-img0010.jpg)
![Western Blot: p97/VCP Antibody [NBP1-81620] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/p97-VCP-Antibody-Western-Blot-NBP1-81620-img0007.jpg)
![Immunoprecipitation: p97/VCP Antibody (5H12) [NBP3-26257] - Immunoprecipitating p97/VCP in U251 whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26257 in U251 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26257 (2ug) + U251 whole cell lysate (500ug). Lane 3: U251 whole cell lysate (10ug).](http://images.novusbio.com/fullsize/nbp3-26257_rabbit-p97-vcp-mab-5h12-26220241322156.jpg "Immunoprecipitation: p97/VCP Antibody (5H12) [NBP3-26257] - Immunoprecipitating p97/VCP in U251 whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26257 in U251 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26257 (2ug) + U251 whole cell lysate (500ug). Lane 3: U251 whole cell lysate (10ug).")
![Immunohistochemistry: p97/VCP Antibody (5H12) [NBP3-26257] - Image of p97/VCP Antibody (5H12) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26257_rabbit-p97-vcp-mab-5h12-26220241535573.jpg)
![Immunohistochemistry: p97/VCP Antibody (5H12) [NBP3-26257] - Image of p97/VCP Antibody (5H12) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26257_rabbit-p97-vcp-mab-5h12-27220248471918.jpg)
![Western Blot: p97/VCP Antibody (5H12) [NBP3-26257] - Positive Western Blot detected in: Hela whole cell lysate, MCF-7 whole cell lysate, U251 whole cell lysate, Rat brain tissue, Rat liver tissue. All lanes: p97/VCP Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 90 kDa Observed band size: 90 kDa](http://images.novusbio.com/fullsize/nbp3-26257_rabbit-p97-vcp-mab-5h12-28220241144267.jpg)
![Immunocytochemistry/Immunofluorescence: p97/VCP Antibody (5H12) [NBP3-26257] - Staining of SY5Y Cells with p97/VCP Antibody (5H12) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26257_rabbit-p97-vcp-mab-5h12-282202412493642.jpg)
![Immunohistochemistry-Paraffin: p97/VCP Antibody [NBP2-15162] - Gastric cancer. VCP antibody [N3C2], Internal dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/p97-VCP-Antibody-Immunohistochemistry-Paraffin-NBP2-15162-img0002.jpg "Immunohistochemistry-Paraffin: p97/VCP Antibody [NBP2-15162] - Gastric cancer. VCP antibody [N3C2], Internal dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Western Blot: p97/VCP Antibody [NBP2-15162] - 50 ug Rat brain lysate/extract 7. 5 % SDS-PAGE gel, antibody dilution: 1:10000.](http://images.novusbio.com/fullsize/p97-VCP-Antibody-Western-Blot-NBP2-15162-img0003.jpg)
![Western Blot: p97/VCP Antibody [NBP2-15162] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 7. 5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/p97-VCP-Antibody-Western-Blot-NBP2-15162-img0004.jpg)
![Western Blot: p97/VCP Antibody [NBP2-15162] - p97/VCP antibody detects p97/VCP protein by Western blot analysis. Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with p97/VCP antibody (NBP2-15162) diluted by 1:1000.](http://images.novusbio.com/fullsize/nbp2-15162_rabbit-polyclonal-p97-vcp-antibody-91020242041275.jpg)
![Western Blot: p97/VCP Antibody (4G9) - BSA Free [NBP1-39850] - HeLa cells (Lane 1), Jurkat cells (Lane 2), MCF7 cells (Lane 3), or mouse splenocytes (Lane 4), using anti-p97/VCP, clone 4G9](http://images.novusbio.com/fullsize/p97-VCP-Antibody-4G9-BSA-Free-Western-Blot-NBP1-39850-img0003.jpg "Western Blot: p97/VCP Antibody (4G9) - BSA Free [NBP1-39850] - HeLa cells (Lane 1), Jurkat cells (Lane 2), MCF7 cells (Lane 3), or mouse splenocytes (Lane 4), using anti-p97/VCP, clone 4G9")
![Immunocytochemistry/Immunofluorescence: p97/VCP Antibody (4G9) - BSA Free [NBP1-39850] - Hela cells stained with purified mouse monoclonal antibody against p97/VCP (clone 4G9), followed by Cy3 conjugated goat anti-mouse IgG and DAPI.](http://images.novusbio.com/fullsize/p97-VCP-Antibody-4G9-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP1-39850-img0004.jpg)
![Immunohistochemistry-Paraffin: p97/VCP Antibody (4G9) - BSA Free [NBP1-39850] - Analysis of anti-VCP antibody with human prostate at concentration 5 ug/ml.](http://images.novusbio.com/fullsize/p97-VCP-Antibody-4G9-BSA-Free-Immunohistochemistry-Paraffin-NBP1-39850-img0001.jpg)
![Western Blot: p97/VCP Antibody (3P8T3) [NBP3-15478] - Western blot analysis of extracts of various cell lines, using p97/VCP Rabbit mAb (NBP3-15478) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/p97-VCP-Antibody-3P8T3-Western-Blot-NBP3-15478-img0004.jpg "Western Blot: p97/VCP Antibody (3P8T3) [NBP3-15478] - Western blot analysis of extracts of various cell lines, using p97/VCP Rabbit mAb (NBP3-15478) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunocytochemistry/Immunofluorescence: p97/VCP Antibody (3P8T3) [NBP3-15478] - Confocal imaging of U-2 OS cells using VCP Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with alpha -Tubulin Mouse mAb (dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-15478_rabbit-p97-vcp-mab-3p8t3-immunocytochemistry-immunofluorescence-25102024123752..png)
![Immunocytochemistry/Immunofluorescence: p97/VCP Antibody (3P8T3) [NBP3-15478] -Confocal imaging of PC-12 cells using VCP Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with alpha -Tubulin Mouse mAb (dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-15478_rabbit-p97-vcp-mab-3p8t3-immunocytochemistry-immunofluorescence-25102024123938.png)
![Immunocytochemistry/Immunofluorescence: p97/VCP Antibody (3P8T3) [NBP3-15478] -Confocal imaging of NIH/3T3 cells using VCP Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with alpha -Tubulin Mouse mAb (dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-15478_rabbit-p97-vcp-mab-3p8t3-immunocytochemistry-immunofluorescence-25102024124410..png)
![Immunocytochemistry/Immunofluorescence: p97/VCP Antibody (Hs-14) [NB500-455] - Intra Acrosomal Protein Antibody (Hs-14) [NB500-455] - Analysis of VCP in acetone-permeabilized human sperms using monoclonal antibody Hs-14 demonstrates its location to the acrosome. (Normal spermiogram shown).](http://images.novusbio.com/fullsize/Intra-Acrosomal-Protein-Antibody-Hs-14-Immunofluorescence-NB500-455-img0001.jpg "Immunocytochemistry/Immunofluorescence: p97/VCP Antibody (Hs-14) [NB500-455] - Intra Acrosomal Protein Antibody (Hs-14) [NB500-455] - Analysis of VCP in acetone-permeabilized human sperms using monoclonal antibody Hs-14 demonstrates its location to the acrosome. (Normal spermiogram shown).")
![SDS-Page: p97/VCP Recombinant Protein [H00007415-P02]](http://images.novusbio.com/fullsize/p97-VCP-Recombinant-Protein-SDS-Page-H00007415-P02-img0001.jpg "SDS-Page: p97/VCP Recombinant Protein [H00007415-P02]")
![Western Blot: p97/VCP Antibody (2B2) [H00007415-M18] - Analysis of VCP expression in A-431 (Cat # L015V1).](http://images.novusbio.com/fullsize/p97-VCP-Antibody-2B2-Western-Blot-H00007415-M18-img0005.jpg "Western Blot: p97/VCP Antibody (2B2) [H00007415-M18] - Analysis of VCP expression in A-431 (Cat # L015V1).")
![Immunocytochemistry/Immunofluorescence: p97/VCP Antibody (2B2) [H00007415-M18] - Analysis of monoclonal antibody to VCP on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/p97-VCP-Antibody-2B2-Immunocytochemistry-Immunofluorescence-H00007415-M18-img0004.jpg)