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![Western Blot: GR/NR3C1 Antibody (BuGR2) [NB300-731] - Analysis of glucocorticoid receptor on mouse liver extract.](http://images.novusbio.com/fullsize/GR-NR3C1-Antibody-BuGR2-Western-Blot-NB300-731-img0017.jpg "Western Blot: GR/NR3C1 Antibody (BuGR2) [NB300-731] - Analysis of glucocorticoid receptor on mouse liver extract.")
![Immunocytochemistry/Immunofluorescence: GR/NR3C1 Antibody (BuGR2) [NB300-731] - Analysis of Glucocorticoid Receptor using Glucocorticoid Receptor Monoclonal Antibody (BuGR2) shows staining in U251 Cells. Glucocorticoid Receptor (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Glucocorticoid Receptor at a dilution of 1:100 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/GR-NR3C1-Antibody-BuGR2-Immunocytochemistry-Immunofluorescence-NB300-731-img0007.jpg)
![Flow Cytometry: GR/NR3C1 Antibody (BuGR2) [NB300-731] - Using the Alexa Fluor 647 direct conjugate An intracellular stain was performed on HeLa cells with GR/NR3C1 (BuGR2) antibody NB300-731AF647 (blue) and a matched isotype control NB600-986AF647 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/GR-NR3C1-Antibody-BuGR2-Flow-Cytometry-NB300-731-img0018.jpg)
![Immunocytochemistry/Immunofluorescence: GR/NR3C1 Antibody (BuGR2) [NB300-731] - Analysis of Glucocorticoid Receptor using Glucocorticoid Receptor Monoclonal Antibody (BuGR2) shows staining in A549 Cells. Glucocorticoid Receptor (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Glucocorticoid Receptor at a dilution of 1:100 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/GR-NR3C1-Antibody-BuGR2-Immunocytochemistry-Immunofluorescence-NB300-731-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: GR/NR3C1 Antibody (BuGR2) [NB300-731] - Analysis of Glucocorticoid Receptor using Glucocorticoid Receptor Monoclonal Antibody (BuGR2) shows staining in HeLa Cells. Glucocorticoid Receptor (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Glucocorticoid Receptor at a dilution of 1:100 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/GR-NR3C1-Antibody-BuGR2-Immunocytochemistry-Immunofluorescence-NB300-731-img0006.jpg)
![Flow Cytometry: GR/NR3C1 Antibody (BuGR2) [NB300-731] - Analysis of GR in Jurkat cells compared to an isotype control (blue).](http://images.novusbio.com/fullsize/GR-NR3C1-Antibody-BuGR2-Flow-Cytometry-NB300-731-img0012.jpg)
![Flow Cytometry: GR/NR3C1 Antibody (BuGR2) [NB300-731] - Analysis of Glucocorticoid Receptor in NIH/3T3 cells compared to an isotype control (blue).](http://images.novusbio.com/fullsize/GR-NR3C1-Antibody-BuGR2-Flow-Cytometry-NB300-731-img0015.jpg)
![Flow Cytometry: GR/NR3C1 Antibody (BuGR2) [NB300-731] - Analysis of Glucocorticoid Receptor in HeLa cells compared to an isotype control (blue).](http://images.novusbio.com/fullsize/GR-NR3C1-Antibody-BuGR2-Flow-Cytometry-NB300-731-img0016.jpg)
![Flow Cytometry: GR/NR3C1 Antibody (BuGR2) [NB300-731] - An intracellular stain was performed on Jurkat cells with GR/NR3C1 (BuGR2) antibody NB300-731PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerthrin.](http://images.novusbio.com/fullsize/GR-NR3C1-Antibody-BuGR2-Flow-Cytometry-NB300-731-img0019.jpg)
![Immunocytochemistry/Immunofluorescence: Glucocorticoid Receptor alpha Antibody [NB300-633] - Analysis of Glucocorticoid Receptor alpha using Glucocorticoid Receptor alpha Polyclonal Antibody shows staining in U251 Cells. Glucocorticoid Receptor alpha (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Glucocorticoid Receptor alpha at a dilution of 1:100 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/Glucocorticoid-Receptor-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NB300-633-img0009.jpg "Immunocytochemistry/Immunofluorescence: Glucocorticoid Receptor alpha Antibody [NB300-633] - Analysis of Glucocorticoid Receptor alpha using Glucocorticoid Receptor alpha Polyclonal Antibody shows staining in U251 Cells. Glucocorticoid Receptor alpha (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Glucocorticoid Receptor alpha at a dilution of 1:100 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.")
![Immunohistochemistry-Paraffin: Glucocorticoid Receptor alpha Antibody [NB300-633] - Both normal and cancer biopsies of deparaffinized Human heart tissues.](http://images.novusbio.com/fullsize/Glucocorticoid-Receptor-alpha-Antibody-Immunohistochemistry-Paraffin-NB300-633-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: Glucocorticoid Receptor alpha Antibody [NB300-633] - Analysis of Glucocorticoid Receptor alpha using Glucocorticoid Receptor alpha Polyclonal Antibody shows staining in A2058 Cells. Glucocorticoid Receptor alpha (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Glucocorticoid Receptor alpha at a dilution of 1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/Glucocorticoid-Receptor-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NB300-633-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Glucocorticoid Receptor alpha Antibody [NB300-633] - Analysis of Glucocorticoid Receptor alpha using Glucocorticoid Receptor alpha Polyclonal Antibody shows staining in Hela Cells. Glucocorticoid Receptor alpha (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Glucocorticoid Receptor alpha at a dilution of 1:100 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody.](http://images.novusbio.com/fullsize/Glucocorticoid-Receptor-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NB300-633-img0008.jpg)
![Immunohistochemistry-Paraffin: Glucocorticoid Receptor alpha Antibody [NB300-633] - Both normal and cancer biopsies of deparaffinized Human cervical carcinoma tissues.](http://images.novusbio.com/fullsize/Glucocorticoid-Receptor-alpha-Antibody-Immunohistochemistry-Paraffin-NB300-633-img0010.jpg)
![Immunohistochemistry-Paraffin: Glucocorticoid Receptor alpha Antibody [NB300-633] - Both normal and cancer biopsies of deparaffinized Human tonsil tissues.](http://images.novusbio.com/fullsize/Glucocorticoid-Receptor-alpha-Antibody-Immunohistochemistry-Paraffin-NB300-633-img0013.jpg)
![Immunohistochemistry-Paraffin: Glucocorticoid Receptor alpha Antibody [NB300-633] - Both normal and cancer biopsies of deparaffinized Human cervical carcinoma tissues.](http://images.novusbio.com/fullsize/Glucocorticoid-Receptor-alpha-Antibody-Immunohistochemistry-Paraffin-NB300-633-img0015.jpg)
![Knockout Validated: GR/NR3C1 Antibody [NBP2-42221] - Wild-type (WT) and Glucocorticoid receptor knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glucocorticoid receptor antibody. HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/GR-NR3C1-Antibody-Western-Blot-NBP2-42221-img0009.jpg "<b>Genetic Strategies Validation. </b>Knockout Validated: GR/NR3C1 Antibody [NBP2-42221] - Wild-type (WT) and Glucocorticoid receptor knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glucocorticoid receptor antibody. HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![<b>Genetic Strategies Validation. </b>Western Blot: GR/NR3C1 Antibody [NBP2-42221] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Glucocorticoid Receptor antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/GR-NR3C1-Antibody-Western-Blot-NBP2-42221-img0012.jpg)
![Immunohistochemistry-Paraffin: GR/NR3C1 Antibody [NBP2-42221] - Human lung cancer. Glucocorticoid Receptor stained by Glucocorticoid Receptor antibody (GR/NR3C1)](http://images.novusbio.com/fullsize/GR-NR3C1-Antibody-Immunohistochemistry-Paraffin-NBP2-42221-img0013.jpg)
![Immunohistochemistry-Paraffin: GR/NR3C1 Antibody [NBP2-42221] - Human colon carcinoma, using Glucocorticoid receptorantibody at 1:250 dilution.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/GR-NR3C1-Antibody-Immunohistochemistry-Paraffin-NBP2-42221-img0006.jpg)
![Immunoprecipitation: GR/NR3C1 Antibody [NBP2-42221] - Sample: 1000 ug HeLa whole cell lysate/extract A. 40 ug HeLa whole cell lysate/extract B. Control with 2.5 ug of preimmune rabbit IgG C. Immunoprecipitation of Glucocorticoid Receptor beta protein by 2. 5 ug of Glucocorticoid Receptor beta antibody 7. 5% SDS-PAGE The immunoprecipitated Glucocorticoid Receptor beta protein was detected by Glucocorticoid Receptor beta antibody diluted at 1:1000](http://images.novusbio.com/fullsize/GR-NR3C1-Antibody-Immunoprecipitation-NBP2-42221-img0007.jpg)
![Immunocytochemistry/ Immunofluorescence: GR/NR3C1 Antibody [NBP2-42221] - GR/NR3C1 antibody detects GR/NR3C1 protein at nucleus by immunofluorescent analysis.Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: GR/NR3C1 stained by GR/NR3C1 antibody (NBP2-42221) diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-42221_rabbit-polyclonal-gr-nr3c1-antibody-91020242075943.jpg)
![Western Blot: GR/NR3C1 Antibody (C.15200010) [NBP2-59202] - Extracts from HeLa cells containing the indicated amounts of GR (from 30 to 240 fmol), and from 5x10^6 Raji or Molt cells were analyzed by Western blot using the antibody against hGR. Figure B. Analysis of extracts from 300,000 HeLa cells with the antibody against hGR (1 ug/mL).](http://images.novusbio.com/fullsize/GR-NR3C1-Antibody-C-15200010-Western-Blot-NBP2-59202-img0001.jpg "Western Blot: GR/NR3C1 Antibody (C.15200010) [NBP2-59202] - Extracts from HeLa cells containing the indicated amounts of GR (from 30 to 240 fmol), and from 5x10^6 Raji or Molt cells were analyzed by Western blot using the antibody against hGR. Figure B. Analysis of extracts from 300,000 HeLa cells with the antibody against hGR (1 ug/mL).")
![Immunocytochemistry/Immunofluorescence: GR/NR3C1 Antibody (C.15200010) [NBP2-59202] - COS-7 cells transiently overexpressing human GR were labeled with the antibody against hGR followed by a biotinylated secondary antibody and peroxidase-labeled avidin. The antibody was used at a concentration of 2.5 ug/mL.](http://images.novusbio.com/fullsize/GR-NR3C1-Antibody-C-15200010-Immunofluorescence-NBP2-59202-img0006.jpg)
![Immunohistochemistry: GR/NR3C1 Antibody (C.15200010) [NBP2-59202] - Immunoreactivity of the monoclonal antibody against hGR in rat CA1 neurons of hippocampus. The antibody was used at 2.5 ug/mL.](http://images.novusbio.com/fullsize/GR-NR3C1-Antibody-C-15200010-Immunohistochemistry-NBP2-59202-img0005.jpg)
![Chromatin Immunoprecipitation: GR/NR3C1 Antibody (C.15200010) [NBP2-59202] - ChIP assays were performed using HeLa cells, the monoclonal antibody directed against GR and optimized PCR primer sets for qPCR. The cells were treated either with ethanol (EtOH, used as a negative control) or triamcinolone acetonide (TA) for 4 hours prior to cell harvesting. ChIP was performed using sheared chromatin from 3 million cells and 5 ug of antibody. QPCR was performed with primers for the human metallothionein promoter (hMTIIA) and for exon 2 of the human myoglobin gene (hmyo ex2), used as a negative control. Figure shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of the human metallothionein IIA promoter by GR.](http://images.novusbio.com/fullsize/GR-NR3C1-Antibody-C-15200010-Chromatin-Immunoprecipitation-NBP2-59202-img0008.jpg)
![Immunoprecipitation: GR/NR3C1 Antibody (C.15200010) [NBP2-59202] - Immunoprecipitation using the antibody against hGR. The glucocorticoid receptor was immunoprecipitated from HeLa cell extracts (5 million HeLa cells in 100 uL IP reaction solution) using 5 ug of the antibody directed against hGR. The IP was followed by Western blot analysis.](http://images.novusbio.com/fullsize/GR-NR3C1-Antibody-C-15200010-Western-Blot-NBP2-59202-img0002.jpg)
![ELISA: GR/NR3C1 Antibody (C.15200010) [NBP2-59202] - Sandwich ELISA The specificity of the antibody directed against hGR was assessed by sandwich ELISA. Figure A: schematic representation of the sandwich ELISA with the monoclonal antibody against hGR. Figure B: ELISA results using the monoclonal antibody against hGR at 0.5 ug/mL. The figure shows an ELISA signal which is proportionally increasing with increasing amounts of recombinant hGR.](http://images.novusbio.com/fullsize/GR-NR3C1-Antibody-C-15200010-ELISA-NBP2-59202-img0003.jpg)
![Western Blot: GR/NR3C1 Antibody (6E6) [NBP2-22174] - Western blot analysis using Glucocorticoid Receptor mouse mAb against Hela cell lysate.](http://images.novusbio.com/fullsize/GR-NR3C1-Antibody-6E6-Western-Blot-NBP2-22174-img0005.jpg "Western Blot: GR/NR3C1 Antibody (6E6) [NBP2-22174] - Western blot analysis using Glucocorticoid Receptor mouse mAb against Hela cell lysate.")
![Immunocytochemistry/Immunofluorescence: GR/NR3C1 Antibody (6E6) [NBP2-22174] - Immunofluorescence analysis of PC-2 cells using Glucocorticoid Receptor mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/GR-NR3C1-Antibody-6E6-Immunocytochemistry-Immunofluorescence-NBP2-22174-img0002.jpg)
![Immunohistochemistry-Paraffin: GR/NR3C1 Antibody (6E6) [NBP2-22174] - Immunohistochemical analysis of paraffin-embedded lung cancer tissues using Glucocorticoid Receptor mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/GR-NR3C1-Antibody-6E6-Immunohistochemistry-Paraffin-NBP2-22174-img0003.jpg)
![Flow Cytometry: GR/NR3C1 Antibody (6E6) [NBP2-22174] - Flow cytometric analysis of K562 cells using Glucocorticoid Receptor mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/GR-NR3C1-Antibody-6E6-Flow-Cytometry-NBP2-22174-img0001.jpg)
![ELISA: GR/NR3C1 Antibody (6E6) [NBP2-22174] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)](http://images.novusbio.com/fullsize/GR-NR3C1-Antibody-6E6-ELISA-NBP2-22174-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: GR/NR3C1 Antibody (59) [NB120-3579] - Analysis of Glucocorticoid Receptor using Glucocorticoid Receptor Polyclonal Antibody (59) shows staining in Hela Cells. Glucocorticoid Receptor (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Glucocorticoid Receptor at a dilution of 1:100 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/GR-NR3C1-Antibody-59-Immunocytochemistry-Immunofluorescence-NB120-3579-img0002.jpg "Immunocytochemistry/Immunofluorescence: GR/NR3C1 Antibody (59) [NB120-3579] - Analysis of Glucocorticoid Receptor using Glucocorticoid Receptor Polyclonal Antibody (59) shows staining in Hela Cells. Glucocorticoid Receptor (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Glucocorticoid Receptor at a dilution of 1:100 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.")
![Immunocytochemistry/Immunofluorescence: GR/NR3C1 Antibody (59) [NB120-3579] - Analysis of Glucocorticoid Receptor using Glucocorticoid Receptor Polyclonal Antibody (59) shows staining in A2058 Cells. Glucocorticoid Receptor (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Glucocorticoid Receptor at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/GR-NR3C1-Antibody-59-Immunocytochemistry-Immunofluorescence-NB120-3579-img0001.jpg)
![Western Blot: GR/NR3C1 Antibody (GT248) [NBP3-13626] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GR/NR3C1 antibody [GT248] (NBP3-13626) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/GR-NR3C1-Antibody-GT248-Western-Blot-NBP3-13626-img0001.jpg "Western Blot: GR/NR3C1 Antibody (GT248) [NBP3-13626] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GR/NR3C1 antibody [GT248] (NBP3-13626) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: GR/NR3C1 Antibody (GT248) [NBP3-13626] - GR/NR3C1 antibody [GT248] detects GR/NR3C1 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. GR/NR3C1 stained by GR/NR3C1 antibody [GT248] (NBP3-13626) diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/GR-NR3C1-Antibody-GT248-Immunohistochemistry-Paraffin-NBP3-13626-img0004.jpg)
![Immunohistochemistry-Paraffin: GR/NR3C1 Antibody (GT248) [NBP3-13626] - GR/NR3C1 antibody [GT248] detects GR/NR3C1 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. GR/NR3C1 stained by GR/NR3C1 antibody [GT248] (NBP3-13626) diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/GR-NR3C1-Antibody-GT248-Immunohistochemistry-Paraffin-NBP3-13626-img0002.jpg)
![Immunohistochemistry-Paraffin: GR/NR3C1 Antibody (GT248) [NBP3-13626] - GR/NR3C1 antibody [GT248] detects GR/NR3C1 protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. GR/NR3C1 stained by GR/NR3C1 antibody [GT248] (NBP3-13626) diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/GR-NR3C1-Antibody-GT248-Immunohistochemistry-Paraffin-NBP3-13626-img0003.jpg)
![Western Blot: GR/NR3C1 Antibody (GT1073) [NBP3-13630] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GR/NR3C1 antibody [GT1073] (NBP3-13630) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/GR-NR3C1-Antibody-GT1073-Western-Blot-NBP3-13630-img0001.jpg "Western Blot: GR/NR3C1 Antibody (GT1073) [NBP3-13630] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GR/NR3C1 antibody [GT1073] (NBP3-13630) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: GR/NR3C1 Antibody (GT1073) [NBP3-13630] - Rat brain. GR/NR3C1 stained by GR/NR3C1 antibody [GT1073] (NBP3-13630) diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/GR-NR3C1-Antibody-GT1073-Immunohistochemistry-Paraffin-NBP3-13630-img0003.jpg)
![Immunohistochemistry-Paraffin: GR/NR3C1 Antibody (GT1073) [NBP3-13630] - Mouse intestine. GR/NR3C1 stained by GR/NR3C1 antibody [GT1073] (NBP3-13630) diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/GR-NR3C1-Antibody-GT1073-Immunohistochemistry-Paraffin-NBP3-13630-img0002.jpg)
![Western Blot: GR/NR3C1 Antibody [NBP1-89733] - Analysis in control (vector only transfected HEK293T lysate) and NR3C1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/GR-NR3C1-Antibody-Western-Blot-NBP1-89733-img0010.jpg "Western Blot: GR/NR3C1 Antibody [NBP1-89733] - Analysis in control (vector only transfected HEK293T lysate) and NR3C1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![Immunocytochemistry/Immunofluorescence: GR/NR3C1 Antibody [NBP1-89733] - Staining of human cell line A-431 shows positivity in nucleus but not nucleoli and cytoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/GR-NR3C1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89733-img0003.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: GR/NR3C1 Antibody [NBP1-89733] - Analysis in human cell lines A-549 and Caco-2 using anti-NR3C1 antibody. Corresponding NR3C1 RNA-seq data are presented for the same cell lines. Loading control: anti-GAPDH.](http://images.novusbio.com/fullsize/GR-NR3C1-Antibody-Western-Blot-NBP1-89733-img0009.jpg)
![Western Blot: GR/NR3C1 Antibody (S04-8C8) [NBP3-14938] - Western blot detection of GR/NR3C1 in K562, 3T3, Hela cell lysates using GR/NR3C1 Rabbit mAb (1:1000 diluted). Predicted band size: 86kDa. Observed band size: 94, 91kDa.](http://images.novusbio.com/fullsize/GR-NR3C1-Antibody-S04-8C8-Western-Blot-NBP3-14938-img0001.jpg "Western Blot: GR/NR3C1 Antibody (S04-8C8) [NBP3-14938] - Western blot detection of GR/NR3C1 in K562, 3T3, Hela cell lysates using GR/NR3C1 Rabbit mAb (1:1000 diluted). Predicted band size: 86kDa. Observed band size: 94, 91kDa.")
![Immunocytochemistry/Immunofluorescence: GR/NR3C1 Antibody (S04-8C8) [NBP3-14938] - Immunofluorescence of GR/NR3C1 (green) in Hela cells using GR/NR3C1 Rabbit mAb at dilution 1/50, and DAPI (blue)](http://images.novusbio.com/fullsize/GR-NR3C1-Antibody-S04-8C8-Immunocytochemistry-Immunofluorescence-NBP3-14938-img0002.jpg)
![Western Blot: GR/NR3C1 Antibody (2K3E8) [NBP3-15789] - Western blot analysis of extracts of various cell lines, using GR/NR3C1 antibody (NBP3-15789) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/GR-NR3C1-Antibody-2K3E8-Western-Blot-NBP3-15789-img0001.jpg "Western Blot: GR/NR3C1 Antibody (2K3E8) [NBP3-15789] - Western blot analysis of extracts of various cell lines, using GR/NR3C1 antibody (NBP3-15789) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Immunoprecipitation: GR/NR3C1 Antibody (2K3E8) [NBP3-15789] - Immunoprecipitation analysis of 300ug extracts of U-251MG cells using 3ug GR/NR3C1 antibody (NBP3-15789). Western blot was performed from the immunoprecipitate using GR/NR3C1 antibody (NBP3-15789) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/GR-NR3C1-Antibody-2K3E8-Immunoprecipitation-NBP3-15789-img0002.jpg)