| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-91258SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF1918-SP | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Allophycocyanin | IC1918A | |||
| Fluorescein | IC1918F | |||
| Phycoerythrin | IC1918P | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | IC1918A | |||
| Carrier Free | IC1918F | |||
| Carrier Free | IC1918P | |||
| Carrier Free | MAB1918-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | 1030-FN-05M | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22113SS | |||
| Azide and BSA Free | NBP2-80737 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70762 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-34633-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | MAB8258-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-48018-0.1mg | |||
| IHC-Prediluted | NBP2-48338 | |||
![Western Blot: Fibronectin Antibody - BSA Free [NBP1-91258] - VSOP observed in perivascular-restricted spinal cord lesions with intact BBB. Immunostaining for laminin (brown) shows vascular endothelium and glia limitans of a perivascular lesion, along with infiltrating cells and VSOP (blue). Image collected and cropped by CiteAb from the following publication (//asn.sagepub.com/lookup/doi/10.1042/AN20120081), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Fibronectin-Antibody---BSA-Free-Western-Blot-NBP1-91258-img0020.jpg "Western Blot: Fibronectin Antibody - BSA Free [NBP1-91258] - VSOP observed in perivascular-restricted spinal cord lesions with intact BBB. Immunostaining for laminin (brown) shows vascular endothelium and glia limitans of a perivascular lesion, along with infiltrating cells and VSOP (blue). Image collected and cropped by CiteAb from the following publication (//asn.sagepub.com/lookup/doi/10.1042/AN20120081), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Fibronectin Antibody - BSA Free [NBP1-91258] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti- NBP1-91258 at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/Fibronectin-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NBP1-91258-img0021.jpg)
![Immunohistochemistry-Frozen: Fibronectin Antibody - BSA Free [NBP1-91258] - Analysis in a 6 month old Alport mouse kidney. Image from a verified customer review.](http://images.novusbio.com/fullsize/Fibronectin-Antibody---BSA-Free-Immunohistochemistry-Frozen-NBP1-91258-img0019.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: Fibronectin Antibody - BSA Free [NBP1-91258] - Nicotinamide (NAM) attenuates unilateral urethral obstruction (UUO)-induced renal interstitial fibrosis. C57BL/6 mice were subjected to UUO surgery or sham operation. Different doses of NAM or saline were intraperitoneally injected an hour before the surgery and daily thereafter. Representative images of Western blot of FN (fibronectin), alpha-SMA (alpha-smooth muscle actin) and GAPDH (loading control). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30993884/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Fibronectin Antibody - BSA Free-Western Blot-NBP1-91258-img0022.jpg)
![Western Blot: Fibronectin Antibody - BSA Free [NBP1-91258] - Analysis of Fibronectin in HepG2 cell lysate.](http://images.novusbio.com/fullsize/Fibronectin-Antibody---BSA-Free-Western-Blot-NBP1-91258-img0003.jpg)
![Western Blot: Fibronectin Antibody - BSA Free [NBP1-91258] - Analysis of Fibronectin in NIH 3T3 cell lysate.](http://images.novusbio.com/fullsize/Fibronectin-Antibody---BSA-Free-Western-Blot-NBP1-91258-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Fibronectin Antibody - BSA Free [NBP1-91258] - Fibronectin antibody was tested in HeLa cells with DyLight 488 (Green). Nuclei and alpha-tubulin were counterstained with DAPI (Blue) and DyLight 550 (Red).](http://images.novusbio.com/fullsize/Fibronectin-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NBP1-91258-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Fibronectin Antibody - BSA Free [NBP1-91258] - NIH-3T3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-Fibronectin at 2 ug/mL overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at 1:500. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at 1:1000 and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Fibronectin-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NBP1-91258-img0012.jpg)
![Immunohistochemistry: Fibronectin Antibody - BSA Free [NBP1-91258] - Analysis of Fibronectin in human renal cancer using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/Fibronectin-Antibody---BSA-Free-Immunohistochemistry-NBP1-91258-img0007.jpg)
![Immunohistochemistry-Paraffin: Fibronectin Antibody - BSA Free [NBP1-91258] - Analysis of Fibronectin in mouse kidney tissue section using anti-Fibronectin antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/Fibronectin-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NBP1-91258-img0010.jpg)
![Immunohistochemistry-Paraffin: Fibronectin Antibody - BSA Free [NBP1-91258] - Analysis of Fibronectin in human oesophageal cancer tissue using anti-Fibronectin antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/Fibronectin-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NBP1-91258-img0011.jpg)
![Immunohistochemistry-Frozen: Fibronectin Antibody - BSA Free [NBP1-91258] - Feline corneal stromal cells. IHC-Fr and IF (Alexa Fluor488) were performed using NBP1-91258 at 1:400. Image captured by epifluorescent microscopy. Image from verified customer review.](http://images.novusbio.com/fullsize/Fibronectin-Antibody---BSA-Free-Immunohistochemistry-Frozen-NBP1-91258-img0013.jpg)
![Immunohistochemistry-Frozen: Fibronectin Antibody - BSA Free [NBP1-91258] - Trabecular meshwork (TM) region of pig eyes. NBP1-91258 Fibronectin antibody was labelled with Alexa Fluor 488 conjugated secondary antibody (Green). DAPI shown as blue. Image from verified customer review.](http://images.novusbio.com/fullsize/Fibronectin-Antibody---BSA-Free-Immunohistochemistry-Frozen-NBP1-91258-img0016.jpg)
![Immunohistochemistry-Frozen: Fibronectin Antibody - BSA Free [NBP1-91258] - Mouse brain cryosections were stained with Fibronectin and anti-rabbit Alexa Fluor 555. Magnification 20x. Image from vrified customer review.](http://images.novusbio.com/fullsize/Fibronectin-Antibody---BSA-Free-Immunohistochemistry-Frozen-NBP1-91258-img0017.jpg)
![Immunohistochemistry-Frozen: Fibronectin Antibody - BSA Free [NBP1-91258] - Staining in mouse carotid artery. Image from a verified customer review.](http://images.novusbio.com/fullsize/Fibronectin-Antibody---BSA-Free-Immunohistochemistry-Frozen-NBP1-91258-img0018.jpg)
![Simple Western: Fibronectin Antibody - BSA Free [NBP1-91258] - Lane view shows a specific band for Fibronectin in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Fibronectin-Antibody---BSA-Free-Simple-Western-NBP1-91258-img0009.jpg)
![Immunocytochemistry/ Immunofluorescence: Fibronectin Antibody - BSA Free [NBP1-91258] - LC depressed both the protein & mRNA level of fibronectin via depletion of peritoneal M2. Values were expressed as the mean ± SD. (A) The overexpression of fibronectin induced by Lactate-4.25% dialysate was evidently downregulated by LC treatment measured by Western blotting; (B) The relative protein level of fibronectin normalized by GAPDH; (C) The mRNA level of fibronectin was depressed by LC treatment; (D) Immunofluorescence staining of fibronectin in the four groups. Blue corresponds to nuclear staining, & red corresponds to fibronectin staining. #p < 0.05 vs. NC & LC group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23685870), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-91258_rabbit-polyclonal-fibronectin-antibody-310202415371918.jpg)
![Western Blot: Fibronectin Antibody - BSA Free [NBP1-91258] - EMT in cancer cells is regulated by m6A levels of mRNAs. a HeLa & HepG2 cells were treated with or without 10 ng/ml TGF-beta for 3 days, the m6A/A ratio of the total mRNA were determined by LC–MS/MS. b Wound healing of wild-type (control) or Mettl3Mut/− cells was recorded (left) & quantitatively analyzed (right). c Wild-type or Mettl3Mut/− cells were allowed to invade for 24 h & tested by CytoSelect™ 24-well Cell Invasion assay kits (8 µm, colorimetric format); d, e mRNA (d) & protein (e) expressions of MMP2, FN, & E-Cad in wild-type & Mettl3Mut/− HeLa cells were measured by qRT-PCR & western blot analysis, respectively. f HeLa cells were transfected with pcDNA/ALKBH5 or a vector control for 48 h, protein expression was determined by western blot analysis (left) & quantitatively analyzed (right). g Wild-type or Mettl3Mut/− cells were treated with or without 10 ng/ml TGF-beta for 3 days, protein expression was determined by western blot analysis (left) & quantitatively analyzed (right). h The expression of METTL3 in liver cancer & its matched adjacent normal tissues of 50 patients from TCGA database. i Correlation between METTL3 & CDH1 in liver cancer patients (n = 364) from TCGA database. j HeLa cells were pretreated with or without Smad2/3 inhibitor SB431542 (10 μM) & then further treated with 10 ng/ml TGF-beta for 3 days, the m6A/A ratio of the total mRNA were determined by LC–MS/MS. Data are presented as means ± SD from three independent experiments. *p < 0.05, **p < 0.01, NS, no significant, by Student’s t test. Red bar = 200 μm Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31061416), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-91258_rabbit-polyclonal-fibronectin-antibody-310202416114750.jpg)
![Western Blot: Fibronectin Antibody - BSA Free [NBP1-91258] - EMT in cancer cells is regulated by m6A levels of mRNAs. a HeLa & HepG2 cells were treated with or without 10 ng/ml TGF-beta for 3 days, the m6A/A ratio of the total mRNA were determined by LC–MS/MS. b Wound healing of wild-type (control) or Mettl3Mut/− cells was recorded (left) & quantitatively analyzed (right). c Wild-type or Mettl3Mut/− cells were allowed to invade for 24 h & tested by CytoSelect™ 24-well Cell Invasion assay kits (8 µm, colorimetric format); d, e mRNA (d) & protein (e) expressions of MMP2, FN, & E-Cad in wild-type & Mettl3Mut/− HeLa cells were measured by qRT-PCR & western blot analysis, respectively. f HeLa cells were transfected with pcDNA/ALKBH5 or a vector control for 48 h, protein expression was determined by western blot analysis (left) & quantitatively analyzed (right). g Wild-type or Mettl3Mut/− cells were treated with or without 10 ng/ml TGF-beta for 3 days, protein expression was determined by western blot analysis (left) & quantitatively analyzed (right). h The expression of METTL3 in liver cancer & its matched adjacent normal tissues of 50 patients from TCGA database. i Correlation between METTL3 & CDH1 in liver cancer patients (n = 364) from TCGA database. j HeLa cells were pretreated with or without Smad2/3 inhibitor SB431542 (10 μM) & then further treated with 10 ng/ml TGF-beta for 3 days, the m6A/A ratio of the total mRNA were determined by LC–MS/MS. Data are presented as means ± SD from three independent experiments. *p < 0.05, **p < 0.01, NS, no significant, by Student’s t test. Red bar = 200 μm Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31061416), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-91258_rabbit-polyclonal-fibronectin-antibody-310202416121971.jpg)
![Western Blot: Fibronectin Antibody - BSA Free [NBP1-91258] - Snail is involved in m6A-regulated EMT in cancer cells. a Overlapping of 2.0-fold m6A expression changes in EMT cells & EMT-related functional genes. b m6A peaks were enriched in CDS & 3′UTRs of SNAI1 genes from m6A RIP-seq data. Squares marked increases of m6A peaks in cancer cells undergoing EMT; c m6A RIP-qPCR analysis of SNAI1 mRNA in the control & EMT undergoing HeLa cells. d Protein expression of Snail in Mettl3Mut/− or AKLBH5 transfected (24 h) HeLa cells & the control. e The wound healing of wild-type or Mettl3Mut/− HeLa cells transfected with or without pcDNA/Snail for 48 h were recorded (left) & quantitatively analyzed (right). f Wild-type or Mettl3Mut/− HeLa cells were transfected with or without pcDNA/Snail for 48 h, expression of Snail, FN & E-Cad were measured by western blot analysis. Data are presented as means ± SD from three independent experiments. *p < 0.05, NS, no significant, by Student’s t test. Red bar = 200μm Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31061416), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-91258_rabbit-polyclonal-fibronectin-antibody-310202416121913.jpg)
![Western Blot: Fibronectin Antibody - BSA Free [NBP1-91258] - EMT in cancer cells is regulated by m6A levels of mRNAs. a HeLa & HepG2 cells were treated with or without 10 ng/ml TGF-beta for 3 days, the m6A/A ratio of the total mRNA were determined by LC–MS/MS. b Wound healing of wild-type (control) or Mettl3Mut/− cells was recorded (left) & quantitatively analyzed (right). c Wild-type or Mettl3Mut/− cells were allowed to invade for 24 h & tested by CytoSelect™ 24-well Cell Invasion assay kits (8 µm, colorimetric format); d, e mRNA (d) & protein (e) expressions of MMP2, FN, & E-Cad in wild-type & Mettl3Mut/− HeLa cells were measured by qRT-PCR & western blot analysis, respectively. f HeLa cells were transfected with pcDNA/ALKBH5 or a vector control for 48 h, protein expression was determined by western blot analysis (left) & quantitatively analyzed (right). g Wild-type or Mettl3Mut/− cells were treated with or without 10 ng/ml TGF-beta for 3 days, protein expression was determined by western blot analysis (left) & quantitatively analyzed (right). h The expression of METTL3 in liver cancer & its matched adjacent normal tissues of 50 patients from TCGA database. i Correlation between METTL3 & CDH1 in liver cancer patients (n = 364) from TCGA database. j HeLa cells were pretreated with or without Smad2/3 inhibitor SB431542 (10 μM) & then further treated with 10 ng/ml TGF-beta for 3 days, the m6A/A ratio of the total mRNA were determined by LC–MS/MS. Data are presented as means ± SD from three independent experiments. *p < 0.05, **p < 0.01, NS, no significant, by Student’s t test. Red bar = 200 μm Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31061416), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-91258_rabbit-polyclonal-fibronectin-antibody-310202416121923.jpg)
![Immunocytochemistry/Immunofluorescence: Fibronectin Antibody (2F4) [NBP2-22113] - Analysis of cultured HepG2 cells using Fibronectin antibody (clone 2F4) at 1:100 dilution. The cells were fixed in formalin and permeabilized using PBS with 0.1% Triton X-100 before incubating them with Fibronectin antibody or the mouse IgG1 isotype control antibody. DyLight 488 conjugated goat anti-mouse IgG (H+L) cross-adsorbed secondary antibody was used at 1: 200 dilution to detect the antigen-primary antibody complexes and the cells were counterstained with DAPI for labeling the nuclei. This Fibronectin antibody generated the expected punctate cytoplasmic-membranous staining and the signal was localized more to the inter-cellular spaces in clustered cells.](http://images.novusbio.com/fullsize/Fibronectin-Antibody-2F4-Immunocytochemistry-Immunofluorescence-NBP2-22113-img0009.jpg "Immunocytochemistry/Immunofluorescence: Fibronectin Antibody (2F4) [NBP2-22113] - Analysis of cultured HepG2 cells using Fibronectin antibody (clone 2F4) at 1:100 dilution. The cells were fixed in formalin and permeabilized using PBS with 0.1% Triton X-100 before incubating them with Fibronectin antibody or the mouse IgG1 isotype control antibody. DyLight 488 conjugated goat anti-mouse IgG (H+L) cross-adsorbed secondary antibody was used at 1: 200 dilution to detect the antigen-primary antibody complexes and the cells were counterstained with DAPI for labeling the nuclei. This Fibronectin antibody generated the expected punctate cytoplasmic-membranous staining and the signal was localized more to the inter-cellular spaces in clustered cells.")
![Western Blot: Fibronectin Antibody (2F4) [NBP2-22113] - Analysis of HeLa (1) and HepG2 (2) whole cell lysates using Fibronectin antibody (2F4) at 2 ug/mL.](http://images.novusbio.com/fullsize/Fibronectin-Antibody-2F4-Western-Blot-NBP2-22113-img0008.jpg)
![Immunohistochemistry-Paraffin: Fibronectin Antibody (2F4) [NBP2-22113] - Analysis of paraffin-embedded stomach cancer tissues using Fibronectin mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Fibronectin-Antibody-2F4-Immunohistochemistry-Paraffin-NBP2-22113-img0006.jpg)
![Flow Cytometry: Fibronectin Antibody (2F4) [NBP2-22113] - Analysis of HeLa cells using Fibronectin mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Fibronectin-Antibody-2F4-Flow-Cytometry-NBP2-22113-img0004.jpg)
![Western Blot: Fibronectin Antibody (2F4) [NBP2-22113] - Analysis using Fibronectin mAb against human Fibronectin (AA: 1965-2176) recombinant protein. Expected MW is 49.6 kDa.](http://images.novusbio.com/fullsize/Fibronectin-Antibody-2F4-Western-Blot-NBP2-22113-img0002.jpg)
![Western Blot: Fibronectin Antibody (2F4) [NBP2-22113] - Analysis using Fibronectin mAb against HEK293 (1) and Fibronectin (AA: 1965-2176)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Fibronectin-Antibody-2F4-Western-Blot-NBP2-22113-img0003.jpg)
![Immunohistochemistry-Paraffin: Fibronectin Antibody (2F4) [NBP2-22113] - Analysis of paraffin-embedded lung cancer tissues using Fibronectin mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Fibronectin-Antibody-2F4-Immunohistochemistry-Paraffin-NBP2-22113-img0005.jpg)
![ELISA: Fibronectin Antibody (2F4) [NBP2-22113] - Red: Control Antigen (100 ng); Purple: Antigen (10 ng); Green: Antigen (50 ng); Blue: Antigen (100 ng).](http://images.novusbio.com/fullsize/Fibronectin-Antibody-2F4-ELISA-NBP2-22113-img0001.jpg)
![Western Blot: Fibronectin Antibody [NBP1-31369] - Untreated (-) and treated (+) HepG2 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Fibronectin antibody [N1N2], N-term.](http://images.novusbio.com/fullsize/Fibronectin-Antibody-Western-Blot-NBP1-31369-img0015.jpg "<b>Biological Strategies Validation. </b>Western Blot: Fibronectin Antibody [NBP1-31369] - Untreated (-) and treated (+) HepG2 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Fibronectin antibody [N1N2], N-term.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Fibronectin/Anastellin Antibody [NBP1-31369] - Analysis of HeLa, using NBP1-31369 at 1:200 dilution.](http://images.novusbio.com/fullsize/Fibronectin-Anastellin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31369-img0007.jpg)
![Immunohistochemistry-Paraffin: Fibronectin/Anastellin Antibody [NBP1-31369] - Paraffin-embedded hepatoma. Fibronectin antibody [N1N2], N-term dilution: 1:500.](http://images.novusbio.com/fullsize/Fibronectin-Anastellin-Antibody-Immunohistochemistry-Paraffin-NBP1-31369-img0010.jpg)
![Western Blot: Fibronectin/Anastellin Antibody [NBP1-31369] - Mouse tissue extracts (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with Fibronectin antibody [N1N2] diluted at 1:1000.](http://images.novusbio.com/fullsize/Fibronectin-Anastellin-Antibody-Western-Blot-NBP1-31369-img0013.jpg)
![Immunoprecipitation: Fibronectin/Anastellin Antibody [NBP1-31369] - N-term immunoprecipitates Fibronectin protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A : 30 ug whole cell lysate/extract of Fibronectin protein expressing HeLa cells B : Control with 3 ug of pre-immune rabbit IgG C : Immunoprecipitation of Fibronectin by 3 ug of Fibronectin antibody [N1N2].](http://images.novusbio.com/fullsize/Fibronectin-Anastellin-Antibody-Immunoprecipitation-NBP1-31369-img0011.jpg)
![ELISA: Fibronectin Antibody [NBP1-31369] - Indirect ELISA analysis was performed by coating plate with 100 uL of recombinant Fibronectin protein at concentration of 10 ug/mL. The coated protein is detected with Fibronectin antibody [N1N2], N-term at rangeing from 0.5 to 140 ng/mL.](http://images.novusbio.com/fullsize/Fibronectin-Antibody-ELISA-NBP1-31369-img0016.jpg)
![ELISA: Fibronectin Antibody [NBP1-31369] - Competitive ELISA detection of human fibronectin in plasma. Recombinant or purified native Fibronectin protein was coated to an ELISA plate at concentration of 10 ug/ml. Human plasma was applied as the competitors and was serial diluted at 1, 20, 200, 2000, 20000, 200000X dilutions. The primary antibody concentration was diluted to 14 ng/mL. Rabbit IgG antibody (HRP) was diluted at 1:2,000 and used to detect the primary antibody](http://images.novusbio.com/fullsize/Fibronectin-Antibody-ELISA-NBP1-31369-img0017.jpg)
![Western Blot: Fibronectin Antibody [NBP1-31369] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Fibronectin antibody [N1N2], N-term (NBP1-31369) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31369_rabbit-polyclonal-fibronectin-antibody-910202420131264.jpg)
![Immunohistochemistry-Paraffin: Fibronectin Antibody [NBP1-31369] - Fibronectin antibody [N1N2], N-term detects Fibronectin protein at cell membrane by immunohistochemical analysis.Sample: Paraffin-embedded rat colon.Fibronectin stained by Fibronectin antibody [N1N2], N-term (NBP1-31369) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-31369_rabbit-polyclonal-fibronectin-antibody-910202420122061.jpg)
![Western Blot: Fibronectin Antibody [NBP1-31369] - Untreated (-) and treated (+) HepG2 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Fibronectin antibody [N1N2], N-term (NBP1-31369) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31369_rabbit-polyclonal-fibronectin-antibody-91020242091482.jpg)
![Immunohistochemistry-Paraffin: Fibronectin Antibody [NBP1-31369] - Fibronectin antibody [N1N2], N-term detects Fibronectin protein at cell membrane by immunohistochemical analysis.Sample: Paraffin-embedded mouse esophagus.Fibronectin stained by Fibronectin antibody [N1N2], N-term (NBP1-31369) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-31369_rabbit-polyclonal-fibronectin-antibody-91020242021940.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Fibronectin Antibody [NBP1-31369] - Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Fibronectin antibody [N1N2], N-term diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp1-31369_rabbit-polyclonal-fibronectin-antibody-161020241940575.jpg)
![Western Blot: Fibronectin/Anastellin Antibody (5C1) [NBP2-45690] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Fibronectin.](http://images.novusbio.com/fullsize/Fibronectin-Anastellin-Antibody-5C1-Western-Blot-NBP2-45690-img0025.jpg "Western Blot: Fibronectin/Anastellin Antibody (5C1) [NBP2-45690] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Fibronectin.")
![Immunohistochemistry: Fibronectin/Anastellin Antibody (5C1) [NBP2-45690] - Analysis of Carcinoma of Human prostate tissue.](http://images.novusbio.com/fullsize/Fibronectin-Anastellin-Antibody-5C1-Immunohistochemistry-NBP2-45690-img0024.jpg)
![Immunohistochemistry: Fibronectin/Anastellin Antibody (5C1) [NBP2-45690] - Analysis of Human breast tissue.](http://images.novusbio.com/fullsize/Fibronectin-Anastellin-Antibody-5C1-Immunohistochemistry-NBP2-45690-img0001.jpg)
![Immunohistochemistry: Fibronectin/Anastellin Antibody (5C1) [NBP2-45690] - Analysis of Adenocarcinoma of Human breast tissue.](http://images.novusbio.com/fullsize/Fibronectin-Anastellin-Antibody-5C1-Immunohistochemistry-NBP2-45690-img0002.jpg)
![Immunohistochemistry: Fibronectin/Anastellin Antibody (5C1) [NBP2-45690] - Analysis of Human colon tissue.](http://images.novusbio.com/fullsize/Fibronectin-Anastellin-Antibody-5C1-Immunohistochemistry-NBP2-45690-img0003.jpg)
![Immunohistochemistry: Fibronectin/Anastellin Antibody (5C1) [NBP2-45690] - Analysis of Adenocarcinoma of Human colon tissue.](http://images.novusbio.com/fullsize/Fibronectin-Anastellin-Antibody-5C1-Immunohistochemistry-NBP2-45690-img0004.jpg)
![Immunohistochemistry: Fibronectin/Anastellin Antibody (5C1) [NBP2-45690] - Analysis of Human Kidney tissue.](http://images.novusbio.com/fullsize/Fibronectin-Anastellin-Antibody-5C1-Immunohistochemistry-NBP2-45690-img0005.jpg)
![Immunohistochemistry: Fibronectin/Anastellin Antibody (5C1) [NBP2-45690] - Analysis of Carcinoma of Human kidney tissue.](http://images.novusbio.com/fullsize/Fibronectin-Anastellin-Antibody-5C1-Immunohistochemistry-NBP2-45690-img0006.jpg)
![Immunohistochemistry: Fibronectin/Anastellin Antibody (5C1) [NBP2-45690] - Analysis of Carcinoma of Human liver tissue.](http://images.novusbio.com/fullsize/Fibronectin-Anastellin-Antibody-5C1-Immunohistochemistry-NBP2-45690-img0007.jpg)
![Immunohistochemistry: Fibronectin/Anastellin Antibody (5C1) [NBP2-45690] - Analysis of Human lung tissue.](http://images.novusbio.com/fullsize/Fibronectin-Anastellin-Antibody-5C1-Immunohistochemistry-NBP2-45690-img0008.jpg)
![Immunohistochemistry: Fibronectin/Anastellin Antibody (5C1) [NBP2-45690] - Analysis of Carcinoma of Human lung tissue.](http://images.novusbio.com/fullsize/Fibronectin-Anastellin-Antibody-5C1-Immunohistochemistry-NBP2-45690-img0009.jpg)
![Immunohistochemistry: Fibronectin/Anastellin Antibody (5C1) [NBP2-45690] - Analysis of Human Ovary tissue.](http://images.novusbio.com/fullsize/Fibronectin-Anastellin-Antibody-5C1-Immunohistochemistry-NBP2-45690-img0010.jpg)
![Immunohistochemistry: Fibronectin/Anastellin Antibody (5C1) [NBP2-45690] - Analysis of Adenocarcinoma of Human ovary tissue.](http://images.novusbio.com/fullsize/Fibronectin-Anastellin-Antibody-5C1-Immunohistochemistry-NBP2-45690-img0011.jpg)
![Immunohistochemistry: Fibronectin/Anastellin Antibody (5C1) [NBP2-45690] - Analysis of Human pancreas tissue.](http://images.novusbio.com/fullsize/Fibronectin-Anastellin-Antibody-5C1-Immunohistochemistry-NBP2-45690-img0012.jpg)
![Immunohistochemistry: Fibronectin/Anastellin Antibody (5C1) [NBP2-45690] - Analysis of Carcinoma of Human pancreas tissue.](http://images.novusbio.com/fullsize/Fibronectin-Anastellin-Antibody-5C1-Immunohistochemistry-NBP2-45690-img0013.jpg)
![Immunohistochemistry: Fibronectin/Anastellin Antibody (5C1) [NBP2-45690] - Analysis of Human thyroid tissue.](http://images.novusbio.com/fullsize/Fibronectin-Anastellin-Antibody-5C1-Immunohistochemistry-NBP2-45690-img0014.jpg)
![Immunohistochemistry: Fibronectin/Anastellin Antibody (5C1) [NBP2-45690] - Analysis of Carcinoma of Human thyroid tissue.](http://images.novusbio.com/fullsize/Fibronectin-Anastellin-Antibody-5C1-Immunohistochemistry-NBP2-45690-img0015.jpg)
![Immunohistochemistry: Fibronectin/Anastellin Antibody (5C1) [NBP2-45690] - Analysis of Human endometrium tissue.](http://images.novusbio.com/fullsize/Fibronectin-Anastellin-Antibody-5C1-Immunohistochemistry-NBP2-45690-img0016.jpg)
![Immunohistochemistry: Fibronectin/Anastellin Antibody (5C1) [NBP2-45690] - Analysis of Adenocarcinoma of Human endometrium tissue.](http://images.novusbio.com/fullsize/Fibronectin-Anastellin-Antibody-5C1-Immunohistochemistry-NBP2-45690-img0017.jpg)
![Immunohistochemistry: Fibronectin/Anastellin Antibody (5C1) [NBP2-45690] - Analysis of Human prostate tissue.](http://images.novusbio.com/fullsize/Fibronectin-Anastellin-Antibody-5C1-Immunohistochemistry-NBP2-45690-img0018.jpg)
![Immunohistochemistry: Fibronectin/Anastellin Antibody (5C1) [NBP2-45690] - Analysis of Human bladder tissue.](http://images.novusbio.com/fullsize/Fibronectin-Anastellin-Antibody-5C1-Immunohistochemistry-NBP2-45690-img0019.jpg)
![Immunohistochemistry: Fibronectin/Anastellin Antibody (5C1) [NBP2-45690] - Analysis of Carcinoma of Human bladder tissue.](http://images.novusbio.com/fullsize/Fibronectin-Anastellin-Antibody-5C1-Immunohistochemistry-NBP2-45690-img0020.jpg)
![Immunohistochemistry: Fibronectin/Anastellin Antibody (5C1) [NBP2-45690] - Analysis of Human lymph node tissue.](http://images.novusbio.com/fullsize/Fibronectin-Anastellin-Antibody-5C1-Immunohistochemistry-NBP2-45690-img0021.jpg)
![Immunohistochemistry: Fibronectin/Anastellin Antibody (5C1) [NBP2-45690] - Analysis of Human lymphoma tissue.](http://images.novusbio.com/fullsize/Fibronectin-Anastellin-Antibody-5C1-Immunohistochemistry-NBP2-45690-img0022.jpg)
![Immunohistochemistry: Fibronectin/Anastellin Antibody (5C1) [NBP2-45690] - Analysis of Human tonsil tissue.](http://images.novusbio.com/fullsize/Fibronectin-Anastellin-Antibody-5C1-Immunohistochemistry-NBP2-45690-img0023.jpg)
![Immunohistochemistry-Paraffin: Fibronectin Antibody (HFN7.1) [NBP2-32847] - Formalin-fixed, paraffin-embedded human Pancreatic Adeno Carcinoma stained with Fibronectin Monoclonal Antibody (HFN7.1).](http://images.novusbio.com/fullsize/Fibronectin-Antibody-HFN7-1-Immunohistochemistry-Paraffin-NBP2-32847-img0001.jpg "Immunohistochemistry-Paraffin: Fibronectin Antibody (HFN7.1) [NBP2-32847] - Formalin-fixed, paraffin-embedded human Pancreatic Adeno Carcinoma stained with Fibronectin Monoclonal Antibody (HFN7.1).")
![Western Blot: Fibronectin Antibody (JF0582) [NBP2-66845] - Analysis of Fibronectin on MG-63 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:40,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/Fibronectin-Antibody-JF0582-Western-Blot-NBP2-66845-img0006.jpg "Western Blot: Fibronectin Antibody (JF0582) [NBP2-66845] - Analysis of Fibronectin on MG-63 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:40,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: Fibronectin Antibody (JF0582) [NBP2-66845] - Staining Fibronectin in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Fibronectin-Antibody-JF0582-Immunocytochemistry-Immunofluorescence-NBP2-66845-img0003.jpg)
![Immunohistochemistry-Paraffin: Fibronectin Antibody (JF0582) [NBP2-66845] - Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Fibronectin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Fibronectin-Antibody-JF0582-Immunohistochemistry-Paraffin-NBP2-66845-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Fibronectin Antibody (JF0582) [NBP2-66845] - Staining Fibronectin in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Fibronectin-Antibody-JF0582-Immunocytochemistry-Immunofluorescence-NBP2-66845-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Fibronectin Antibody (JF0582) [NBP2-66845] - Staining Fibronectin in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Fibronectin-Antibody-JF0582-Immunocytochemistry-Immunofluorescence-NBP2-66845-img0002.jpg)
![Immunohistochemistry-Paraffin: Fibronectin Antibody (JF0582) [NBP2-66845] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Fibronectin antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Fibronectin-Antibody-JF0582-Immunohistochemistry-Paraffin-NBP2-66845-img0004.jpg)
![Immunohistochemistry-Paraffin: Fibronectin Antibody (JF0582) [NBP2-66845] - Analysis of paraffin-embedded human gastric carcinoma tissue using anti-Fibronectin antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Fibronectin-Antibody-JF0582-Immunohistochemistry-Paraffin-NBP2-66845-img0005.jpg)
![Western Blot: Fibronectin Antibody (CL3730) [NBP2-61633] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10 Lane 2: Human cell line U-87 MG](http://images.novusbio.com/fullsize/Fibronectin-Anastellin-Antibody-CL3730-Western-Blot-NBP2-61633-img0001.jpg "Western Blot: Fibronectin Antibody (CL3730) [NBP2-61633] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10 Lane 2: Human cell line U-87 MG")
![Immunohistochemistry-Paraffin: Fibronectin Antibody (CL3730) [NBP2-61633] - Staining of human pancreas shows absence of immunoreactivity in glandular cells (negative control).](http://images.novusbio.com/fullsize/Fibronectin-Anastellin-Antibody-CL3730-Immunohistochemistry-Paraffin-NBP2-61633-img0010.jpg)
![Immunohistochemistry-Paraffin: Fibronectin Antibody (CL3730) [NBP2-61633] - Staining of human endometrium shows strong immunoreactivity in endometrial stroma and basement membrane of glandular cells.](http://images.novusbio.com/fullsize/Fibronectin-Anastellin-Antibody-CL3730-Immunohistochemistry-Paraffin-NBP2-61633-img0003.jpg)
![Immunohistochemistry-Paraffin: Fibronectin Antibody (CL3730) [NBP2-61633] - Staining of human placenta shows strong immunoreactivity in chorionic villi.](http://images.novusbio.com/fullsize/Fibronectin-Anastellin-Antibody-CL3730-Immunohistochemistry-Paraffin-NBP2-61633-img0004.jpg)
![Immunohistochemistry-Paraffin: Fibronectin Antibody (CL3730) [NBP2-61633] - Staining of human testis shows immunoreactivity in the connective tissue surrounding seminiferous tubules.](http://images.novusbio.com/fullsize/Fibronectin-Anastellin-Antibody-CL3730-Immunohistochemistry-Paraffin-NBP2-61633-img0005.jpg)
![Immunohistochemistry-Paraffin: Fibronectin Antibody (CL3730) [NBP2-61633] - Staining of human prostate shows moderate positivity in smooth muscle.](http://images.novusbio.com/fullsize/Fibronectin-Anastellin-Antibody-CL3730-Immunohistochemistry-Paraffin-NBP2-61633-img0006.jpg)
![Immunohistochemistry-Paraffin: Fibronectin Antibody (CL3730) [NBP2-61633] - Staining of human small intestine shows strong immunoreactivity in the connective tissue.](http://images.novusbio.com/fullsize/Fibronectin-Anastellin-Antibody-CL3730-Immunohistochemistry-Paraffin-NBP2-61633-img0007.jpg)
![Immunohistochemistry-Paraffin: Fibronectin Antibody (CL3730) [NBP2-61633] - Staining of human breast cancer shows strong immunoreactivity in tumor stroma.](http://images.novusbio.com/fullsize/Fibronectin-Anastellin-Antibody-CL3730-Immunohistochemistry-Paraffin-NBP2-61633-img0008.jpg)
![Immunohistochemistry-Paraffin: Fibronectin Antibody (CL3730) [NBP2-61633] - Staining of human liver cancer shows strong positivity in tumor.](http://images.novusbio.com/fullsize/Fibronectin-Anastellin-Antibody-CL3730-Immunohistochemistry-Paraffin-NBP2-61633-img0009.jpg)
![Immunocytochemistry: Fibronectin/Anastellin Antibody (960632) [MAB8258] - Fibronectin/Anastellin was detected in immersion fixed HepG2 cell using Fibronectin/Anastellin Monoclonal Antibody at 3 ug/ml dilution. Cells were stained using Donkey anti-Mouse IgG Secondary Antibody [NL557] (red) and counterstained with DAPI (blue). Specific staining was localized to cytoplasmic.](http://images.novusbio.com/fullsize/Fibronectin-Anastellin-Antibody-960632-Immunocytochemistry-MAB8258-img0002.jpg "Immunocytochemistry: Fibronectin/Anastellin Antibody (960632) [MAB8258] - Fibronectin/Anastellin was detected in immersion fixed HepG2 cell using Fibronectin/Anastellin Monoclonal Antibody at 3 ug/ml dilution. Cells were stained using Donkey anti-Mouse IgG Secondary Antibody [NL557] (red) and counterstained with DAPI (blue). Specific staining was localized to cytoplasmic.")
![Immunohistochemistry: Fibronectin/Anastellin Antibody (960632) [MAB8258] - Anastellin was detected in immersion fixed paraffin-embedded sections of human liver cancer using Anastellin Monoclonal Antibody at 5 ug/ml. Tissue was stained using Mouse IgG VisUCyte HRP Polymer Antibody (brown) and counterstained with hematoxylin (blue). Specific staining was localized to extracellular matrix.](http://images.novusbio.com/fullsize/Fibronectin-Anastellin-Antibody-960632-Immunohistochemistry-MAB8258-img0001.jpg)
![Immunohistochemistry-Paraffin: Fibronectin Antibody (568) [NBP2-45018] - Human Renal Cell Carcinoma stained with Fibronectin Monoclonal Antibody (568).](http://images.novusbio.com/fullsize/Fibronectin-Antibody-568-Immunohistochemistry-Paraffin-NBP2-45018-img0001.jpg "Immunohistochemistry-Paraffin: Fibronectin Antibody (568) [NBP2-45018] - Human Renal Cell Carcinoma stained with Fibronectin Monoclonal Antibody (568).")