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![Western Blot: Dysferlin Antibody (JF05-54) [NBP2-66814] - Analysis of Dysferlin on human skeletal muscle lysates using anti-Dysferlin antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/Dysferlin-Antibody-JF05-54-Western-Blot-NBP2-66814-img0005.jpg "Western Blot: Dysferlin Antibody (JF05-54) [NBP2-66814] - Analysis of Dysferlin on human skeletal muscle lysates using anti-Dysferlin antibody at 1/1,000 dilution.")
![Immunocytochemistry/Immunofluorescence: Dysferlin Antibody (JF05-54) [NBP2-66814] - Staining Dysferlin in SW480 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Dysferlin-Antibody-JF05-54-Immunocytochemistry-Immunofluorescence-NBP2-66814-img0006.jpg)
![Immunohistochemistry-Paraffin: Dysferlin Antibody (JF05-54) [NBP2-66814] - Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Dysferlin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Dysferlin-Antibody-JF05-54-Immunohistochemistry-Paraffin-NBP2-66814-img0007.jpg)
![Immunohistochemistry-Paraffin: Dysferlin Antibody (JF05-54) [NBP2-66814] - Analysis of paraffin-embedded human spleen tissue using anti-Dysferlin antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Dysferlin-Antibody-JF05-54-Immunohistochemistry-Paraffin-NBP2-66814-img0001.jpg)
![Immunohistochemistry-Paraffin: Dysferlin Antibody (JF05-54) [NBP2-66814] - Analysis of paraffin-embedded mouse heart tissue using anti-Dysferlin antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Dysferlin-Antibody-JF05-54-Immunohistochemistry-Paraffin-NBP2-66814-img0002.jpg)
![Immunohistochemistry-Paraffin: Dysferlin Antibody (JF05-54) [NBP2-66814] - Analysis of paraffin-embedded mouse skeletal muscle tissue using anti-Dysferlin antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Dysferlin-Antibody-JF05-54-Immunohistochemistry-Paraffin-NBP2-66814-img0003.jpg)
![Immunohistochemistry-Paraffin: Dysferlin Antibody (JF05-54) [NBP2-66814] - Analysis of paraffin-embedded mouse spleen tissue using anti-Dysferlin antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Dysferlin-Antibody-JF05-54-Immunohistochemistry-Paraffin-NBP2-66814-img0004.jpg)
![Immunohistochemistry-Paraffin: Dysferlin Antibody [NBP1-84695] - Staining of human testis shows moderate membranous positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Dysferlin-Antibody-Immunohistochemistry-Paraffin-NBP1-84695-img0015.jpg "Immunohistochemistry-Paraffin: Dysferlin Antibody [NBP1-84695] - Staining of human testis shows moderate membranous positivity in cells in seminiferous ducts.")
![Immunohistochemistry-Paraffin: Dysferlin Antibody [NBP1-84695] - Staining of human skeletal muscle shows high expression.](http://images.novusbio.com/fullsize/Dysferlin-Antibody-Immunohistochemistry-Paraffin-NBP1-84695-img0005.jpg)
![Immunohistochemistry-Paraffin: Dysferlin Antibody [NBP1-84695] - Staining of human skin shows low expression as expected.](http://images.novusbio.com/fullsize/Dysferlin-Antibody-Immunohistochemistry-Paraffin-NBP1-84695-img0006.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Dysferlin Antibody [NBP1-84695] - Staining in human skeletal muscle and skin tissues using anti-DYSF antibody. Corresponding DYSF RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Dysferlin-Antibody-Immunohistochemistry-Paraffin-NBP1-84695-img0007.jpg)
![Immunohistochemistry-Paraffin: Dysferlin Antibody [NBP1-84695] - Staining of human kidney.](http://images.novusbio.com/fullsize/Dysferlin-Antibody-Immunohistochemistry-Paraffin-NBP1-84695-img0009.jpg)
![Immunohistochemistry-Paraffin: Dysferlin Antibody [NBP1-84695] - Staining of human testis.](http://images.novusbio.com/fullsize/Dysferlin-Antibody-Immunohistochemistry-Paraffin-NBP1-84695-img0010.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Dysferlin Antibody [NBP1-84695] - Analysis in human skeletal muscle and skin tissues. Corresponding Dysferlin RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Dysferlin-Antibody-Immunohistochemistry-Paraffin-NBP1-84695-img0011.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Dysferlin Antibody [NBP1-84695] - Staining of human kidney, skeletal muscle, skin and testis using Anti-Dysferlin antibody NBP1-84695 (A) shows similar protein distribution across tissues to independent antibody NBP1-84696 (B).](http://images.novusbio.com/fullsize/Dysferlin-Antibody-Immunohistochemistry-Paraffin-NBP1-84695-img0012.jpg)
![Immunohistochemistry-Paraffin: Dysferlin Antibody [NBP1-84695] - Staining of human kidney shows moderate to strong membranous positivity in cells in glomeruli and cells in tubules.](http://images.novusbio.com/fullsize/Dysferlin-Antibody-Immunohistochemistry-Paraffin-NBP1-84695-img0013.jpg)
![Immunohistochemistry-Paraffin: Dysferlin Antibody [NBP1-84695] - Staining of human skin shows no positivity in squamous epithelial cells as expected.](http://images.novusbio.com/fullsize/Dysferlin-Antibody-Immunohistochemistry-Paraffin-NBP1-84695-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: Dysferlin Antibody [NBP1-84696] - Staining of human cell line RH-30 shows localization to plasma membrane & microtubule organizing center.](http://images.novusbio.com/fullsize/Dysferlin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84696-img0004.jpg "Immunocytochemistry/Immunofluorescence: Dysferlin Antibody [NBP1-84696] - Staining of human cell line RH-30 shows localization to plasma membrane & microtubule organizing center.")
![Immunohistochemistry-Paraffin: Dysferlin Antibody [NBP1-84696] - Staining of human testis shows moderate membranous positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Dysferlin-Antibody-Immunohistochemistry-Paraffin-NBP1-84696-img0016.jpg)
![Immunohistochemistry-Paraffin: Dysferlin Antibody [NBP1-84696] - Staining of human skin shows low expression as expected.](http://images.novusbio.com/fullsize/Dysferlin-Antibody-Immunohistochemistry-Paraffin-NBP1-84696-img0007.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Dysferlin Antibody [NBP1-84696] - Analysis in human skeletal muscle and skin tissues. Corresponding Dysferlin RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Dysferlin-Antibody-Immunohistochemistry-Paraffin-NBP1-84696-img0012.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Dysferlin Antibody [NBP1-84696] - Staining of human kidney, skeletal muscle, skin and testis using Anti-Dysferlin antibody NBP1-84696 (A) shows similar protein distribution across tissues to independent antibody NBP1-84695 (B).](http://images.novusbio.com/fullsize/Dysferlin-Antibody-Immunohistochemistry-Paraffin-NBP1-84696-img0013.jpg)
![Immunohistochemistry-Paraffin: Dysferlin Antibody [NBP1-84696] - Staining of human kidney shows moderate to strong membranous positivity in cells in glomeruli and cells in tubules.](http://images.novusbio.com/fullsize/Dysferlin-Antibody-Immunohistochemistry-Paraffin-NBP1-84696-img0014.jpg)
![Immunohistochemistry-Paraffin: Dysferlin Antibody [NBP1-84696] - Staining of human skeletal muscle shows strong cytoplasmic and membranous positivity in myocytes.](http://images.novusbio.com/fullsize/Dysferlin-Antibody-Immunohistochemistry-Paraffin-NBP1-84696-img0015.jpg)
![Immunohistochemistry-Paraffin: Dysferlin Antibody (CL10888) [NBP3-07994] - Analysis in human spleen and skin tissues using NBP3-07994 antibody. Corresponding Dysferlin RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Dysferlin-Antibody-CL10888-Immunohistochemistry-Paraffin-NBP3-07994-img0001.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Dysferlin Antibody (CL10888) [NBP3-07994] - Analysis in human spleen and skin tissues using NBP3-07994 antibody. Corresponding Dysferlin RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Dysferlin Antibody (CL10888) [NBP3-07994] - Analysis in human cell line RH-30.](http://images.novusbio.com/fullsize/Dysferlin-Antibody-CL10888-Western-Blot-NBP3-07994-img0006.jpg)
![Immunohistochemistry-Paraffin: Dysferlin Antibody (CL10888) [NBP3-07994] - Staining of human spleen shows moderate positivity in cells in red pulp.](http://images.novusbio.com/fullsize/Dysferlin-Antibody-CL10888-Immunohistochemistry-Paraffin-NBP3-07994-img0005.jpg)
![Immunohistochemistry-Paraffin: Dysferlin Antibody (CL10888) [NBP3-07994] - Staining of human lymph node shows strong membranous positivity in non-germinal center cells and endothelial cells.](http://images.novusbio.com/fullsize/Dysferlin-Antibody-CL10888-Immunohistochemistry-Paraffin-NBP3-07994-img0002.jpg)
![Immunohistochemistry-Paraffin: Dysferlin Antibody (CL10888) [NBP3-07994] - Staining of human placenta shows moderate membranous positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/Dysferlin-Antibody-CL10888-Immunohistochemistry-Paraffin-NBP3-07994-img0003.jpg)
![Immunohistochemistry-Paraffin: Dysferlin Antibody (CL10888) [NBP3-07994] - Staining of human skin shows no positivity in keratinocytes as expected.](http://images.novusbio.com/fullsize/Dysferlin-Antibody-CL10888-Immunohistochemistry-Paraffin-NBP3-07994-img0004.jpg)
![Western Blot: Dysferlin Antibody (SR2944) [NBP3-27723] - Western blot analysis of Dysferlin expression in Mouse skeletal muscle tissue lysate.](http://images.novusbio.com/fullsize/nbp3-27723_rabbit-dysferlin-mab-sr2944-75202410133441.jpg "Western Blot: Dysferlin Antibody (SR2944) [NBP3-27723] - Western blot analysis of Dysferlin expression in Mouse skeletal muscle tissue lysate.")
![Western Blot: Dysferlin Antibody (3U1P9) [NBP3-15781] - Western blot analysis of extracts of RD cells, using Dysferlin Rabbit mAb (NBP3-15781) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/Dysferlin-Antibody-3U1P9-Western-Blot-NBP3-15781-img0002.jpg "Western Blot: Dysferlin Antibody (3U1P9) [NBP3-15781] - Western blot analysis of extracts of RD cells, using Dysferlin Rabbit mAb (NBP3-15781) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Immunohistochemistry-Paraffin: Dysferlin Antibody (3U1P9) [NBP3-15781] - Immunohistochemistry of paraffin-embedded human placenta using Dysferlin Rabbit mAb (NBP3-15781) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Dysferlin-Antibody-3U1P9-Immunohistochemistry-Paraffin-NBP3-15781-img0001.jpg)
![ELISA: Human Dysferlin ELISA Kit (Colorimetric) [NBP3-31476] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-31476_human-dysferlin-elisa-kit-colorimetric-206202415351048.png "ELISA: Human Dysferlin ELISA Kit (Colorimetric) [NBP3-31476] - Standard Curve Reference")
![ELISA: Human Dysferlin - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-31477] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-31477_human-dysferlin-ready-to-use-elisa-kit-colorimetric-206202415314823.png "ELISA: Human Dysferlin - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-31477] - Standard Curve Reference")