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![Immunohistochemistry-Paraffin: Amphiphysin/AMPH Antibody [NBP1-87561] - Staining in human cerebral cortex and tonsil tissues using anti-AMPH antibody. Corresponding AMPH RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Amphiphysin-AMPH-Antibody-Immunohistochemistry-Paraffin-NBP1-87561-img0011.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Amphiphysin/AMPH Antibody [NBP1-87561] - Staining in human cerebral cortex and tonsil tissues using anti-AMPH antibody. Corresponding AMPH RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Amphiphysin/AMPH Antibody [NBP1-87561] - Staining of human cerebral cortex, colon, kidney and tonsil using Anti-AMPH antibody NBP1-87561 (A) shows similar protein distribution across tissues to independent antibody NBP1-86033 (B).](http://images.novusbio.com/fullsize/Amphiphysin-AMPH-Antibody-Immunohistochemistry-Paraffin-NBP1-87561-img0013.jpg)
![Western Blot: Amphiphysin/AMPH Antibody [NBP1-87561] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human Cerebral Cortex tissue](http://images.novusbio.com/fullsize/Amphiphysin-AMPH-Antibody-Western-Blot-NBP1-87561-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Amphiphysin/AMPH Antibody [NBP1-87561] - Staining of human cell line U-251 MG shows localization to plasma membrane and cytosol.](http://images.novusbio.com/fullsize/Amphiphysin-AMPH-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87561-img0012.jpg)
![Immunohistochemistry-Paraffin: Amphiphysin/AMPH Antibody [NBP1-87561] - Staining of human kidney.](http://images.novusbio.com/fullsize/Amphiphysin-AMPH-Antibody-Immunohistochemistry-Paraffin-NBP1-87561-img0016.jpg)
![Western Blot: Amphiphysin/AMPH Antibody [NBP1-87561] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10 Lane 2: Mouse Cerebral Cortex tissue](http://images.novusbio.com/fullsize/Amphiphysin-AMPH-Antibody-Western-Blot-NBP1-87561-img0005.jpg)
![Immunohistochemistry-Paraffin: Amphiphysin/AMPH Antibody [NBP1-87561] - Staining of human cerebral cortex shows high expression.](http://images.novusbio.com/fullsize/Amphiphysin-AMPH-Antibody-Immunohistochemistry-Paraffin-NBP1-87561-img0009.jpg)
![Immunohistochemistry-Paraffin: Amphiphysin/AMPH Antibody [NBP1-87561] - Staining of human tonsil shows low expression as expected.](http://images.novusbio.com/fullsize/Amphiphysin-AMPH-Antibody-Immunohistochemistry-Paraffin-NBP1-87561-img0010.jpg)
![Immunohistochemistry-Paraffin: Amphiphysin/AMPH Antibody [NBP1-87561] - Staining of human colon.](http://images.novusbio.com/fullsize/Amphiphysin-AMPH-Antibody-Immunohistochemistry-Paraffin-NBP1-87561-img0014.jpg)
![Western Blot: Amphiphysin/AMPH Antibody [NBP1-86033] - Staining of human cerebral cortex, colon, kidney and tonsil using Anti-AMPH antibody NBP1-86033 (A) shows similar protein distribution across tissues to independent antibody NBP1-87561 (B).](http://images.novusbio.com/fullsize/Amphiphysin-AMPH-Antibody-Western-Blot-NBP1-86033-img0010.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Amphiphysin/AMPH Antibody [NBP1-86033] - Staining of human cerebral cortex, colon, kidney and tonsil using Anti-AMPH antibody NBP1-86033 (A) shows similar protein distribution across tissues to independent antibody NBP1-87561 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: Amphiphysin/AMPH Antibody [NBP1-86033] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.](http://images.novusbio.com/fullsize/Amphiphysin-AMPH-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-86033-img0005.jpg)
![Immunohistochemistry-Paraffin: Amphiphysin/AMPH Antibody [NBP1-86033] - Staining of human colon.](http://images.novusbio.com/fullsize/Amphiphysin-AMPH-Antibody-Immunohistochemistry-Paraffin-NBP1-86033-img0012.jpg)
![Western Blot: Amphiphysin/AMPH Antibody [NBP1-86033] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-34](http://images.novusbio.com/fullsize/Amphiphysin-AMPH-Antibody-Western-Blot-NBP1-86033-img0004.jpg)
![Immunohistochemistry-Paraffin: Amphiphysin/AMPH Antibody [NBP1-86033] - Staining of human cerebellum shows strong cytoplasmic positivity in purkinje cells, cells of molecular and granular layer.](http://images.novusbio.com/fullsize/Amphiphysin-AMPH-Antibody-Immunohistochemistry-Paraffin-NBP1-86033-img0006.jpg)
![Immunohistochemistry-Paraffin: Amphiphysin/AMPH Antibody [NBP1-86033] - Staining of human cerebral cortex shows high expression.](http://images.novusbio.com/fullsize/Amphiphysin-AMPH-Antibody-Immunohistochemistry-Paraffin-NBP1-86033-img0007.jpg)
![Immunohistochemistry-Paraffin: Amphiphysin/AMPH Antibody [NBP1-86033] - Staining of human tonsil shows low expression as expected.](http://images.novusbio.com/fullsize/Amphiphysin-AMPH-Antibody-Immunohistochemistry-Paraffin-NBP1-86033-img0008.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Amphiphysin/AMPH Antibody [NBP1-86033] - Staining in human cerebral cortex and tonsil tissues using anti-AMPH antibody. Corresponding AMPH RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Amphiphysin-AMPH-Antibody-Immunohistochemistry-Paraffin-NBP1-86033-img0009.jpg)
![Immunohistochemistry-Paraffin: Amphiphysin/AMPH Antibody [NBP1-86033] - Staining of human kidney.](http://images.novusbio.com/fullsize/Amphiphysin-AMPH-Antibody-Immunohistochemistry-Paraffin-NBP1-86033-img0011.jpg)
![Immunohistochemistry-Paraffin: Amphiphysin/AMPH Antibody [NBP1-32505] - Paraffin-embedded mouse brain; antibody dilution 1:500.](http://images.novusbio.com/fullsize/Amphiphysin-AMPH-Antibody-Immunohistochemistry-Paraffin-NBP1-32505-img0012.jpg "Immunohistochemistry-Paraffin: Amphiphysin/AMPH Antibody [NBP1-32505] - Paraffin-embedded mouse brain; antibody dilution 1:500.")
![Immunohistochemistry: Amphiphysin/AMPH Antibody [NBP1-32505] - Paraffin-embedded U87 xenograft. Antibody at dilution of 1:500.](http://images.novusbio.com/fullsize/Amphiphysin-AMPH-Antibody-Immunohistochemistry-NBP1-32505-img0009.jpg)
![Immunohistochemistry-Paraffin: Amphiphysin/AMPH Antibody [NBP1-32505] - Paraffin-embedded NCIN87 xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/Amphiphysin-AMPH-Antibody-Immunohistochemistry-Paraffin-NBP1-32505-img0010.jpg)
![Immunohistochemistry-Paraffin: Amphiphysin/AMPH Antibody [NBP1-32505] - Paraffin-embedded RT2 xenograft; antibody dilution 1:500.](http://images.novusbio.com/fullsize/Amphiphysin-AMPH-Antibody-Immunohistochemistry-Paraffin-NBP1-32505-img0011.jpg)
![Western Blot: Amphiphysin/AMPH Antibody [NBP1-32505] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Amphiphysin/AMPH antibody [N1N2], N-term (NBP1-32505) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32505_rabbit-polyclonal-amphiphysin-amph-antibody-91020242014696.jpg)
![Immunocytochemistry/ Immunofluorescence: Amphiphysin/AMPH Antibody [NBP1-32505] - Amphiphysin/AMPH antibody [N1N2], N-term detects Amphiphysin/AMPH protein by immunofluorescent analysis.Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Amphiphysin/AMPH stained by Amphiphysin/AMPH antibody [N1N2], N-term (NBP1-32505) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp1-32505_rabbit-polyclonal-amphiphysin-amph-antibody-9102024204124.jpg)
![Western Blot: Amphiphysin/AMPH Antibody [NBP1-32505] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Amphiphysin/AMPH antibody [N1N2], N-term (NBP1-32505) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32505_rabbit-polyclonal-amphiphysin-amph-antibody-91020242001078.jpg)
![Western Blot: Amphiphysin/AMPH Antibody [NB100-40788] - Western blot analysis of Amphiphysin using NB100-40788 at 0.5ug/ml in Mouse Brain lysate (35ug protein in RIPA buffer).](http://images.novusbio.com/fullsize/Amphiphysin-AMPH-Antibody-Western-Blot-NB100-40788-img0006.jpg "Western Blot: Amphiphysin/AMPH Antibody [NB100-40788] - Western blot analysis of Amphiphysin using NB100-40788 at 0.5ug/ml in Mouse Brain lysate (35ug protein in RIPA buffer).")
![Immunohistochemistry-Paraffin: Amphiphysin/AMPH Antibody [NB100-40788] - (2.5ug/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Amphiphysin-AMPH-Antibody-Immunohistochemistry-Paraffin-NB100-40788-img0005.jpg)
![SDS-Page: Recombinant Human Amphiphysin/AMPH Protein [H00000273-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00000273-P01-1.jpg "SDS-Page: Recombinant Human Amphiphysin/AMPH Protein [H00000273-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![ELISA: Human Amphiphysin/AMPH ELISA Kit (Colorimetric) [NBP2-68201] - Samples were spiked with high concentrations of Human Amphiphysin/AMPH and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-68201_human-amphiphysin-amph-elisa-kit-colorimetric-132202416251779.jpg "ELISA: Human Amphiphysin/AMPH ELISA Kit (Colorimetric) [NBP2-68201] - Samples were spiked with high concentrations of Human Amphiphysin/AMPH and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human Amphiphysin/AMPH ELISA Kit (Colorimetric) [NBP2-68201] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-68201_human-amphiphysin-amph-elisa-kit-colorimetric-432024955045.jpg)
![Western Blot: Amphiphysin/AMPH Overexpression Lysate [NBL1-07507] - Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Amphiphysin.](http://images.novusbio.com/fullsize/Amphiphysin-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-07507-img0002.jpg "Western Blot: Amphiphysin/AMPH Overexpression Lysate [NBL1-07507] - Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Amphiphysin.")