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Septin-4 Antibody

Images

 
Western Blot: Septin-4 Antibody [NBP1-52095] - Staining of Human Heart lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Septin-4 Antibody [NBP1-52095] - Staining of paraffin embedded Human Cerebellum. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Flow Cytometry: Septin-4 Antibody [NBP1-52095] - Staining of paraffin embedded Human Cerebellum with antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Septin-4 Antibody Summary

Immunogen
Peptide with sequence C-RNKLTRESGTD corresponding to internal region according to NP_004565.1, NP_536341.1, NP_001185642.1.
Epitope
C-RNKLTRESGTD
Specificity
This antibody is expected to recognize isoform 1 (NP_004565.1), 3 (NP_536341.1) and 4 (NP_001185642.1). Amino acid numbering in name refers to NP_004565.1 sequence.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SEPTIN4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 10 ug/mL
  • Immunohistochemistry 3.75 ug/mL
  • Immunohistochemistry-Paraffin 3.75 ug/mL
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 1 - 3 ug/mL
Publications
Read Publication using NBP1-52095.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Septin-4 Antibody

  • Apoptosis-related protein in the TGF-beta signaling pathway
  • ARTSPeanut-like protein 2
  • Bradeion beta
  • BRADEION
  • Brain protein H5
  • CE5B3
  • Cell division control-related protein 2
  • Cerebral protein 7
  • H5
  • hCDCREL-2SEP4
  • hucep-7
  • MART
  • peanut-like 2 (Drosophila)
  • peanut-like 2
  • PNUTL2CE5B3 beta
  • septin 4
  • septin-4
  • septin-M

Background

This gene is a member of the septin gene family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse and appear to regulate cytoskeletal organization. The protein encoded by this gene is thought to be part of a complex involved in cytokinesis. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Septin-4 Antibody (NBP1-52095)(1)


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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SEPTIN4
Entrez
Uniprot