![Western Blot: Septin-4 Antibody [NBP1-52096] - (0.3ug/ml) staining of 1Human Cerebellum (A) and Mouse Brain (B) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Septin-4-Antibody-Western-Blot-NBP1-52096-img0002.jpg "Western Blot: Septin-4 Antibody [NBP1-52096] - (0.3ug/ml) staining of 1Human Cerebellum (A) and Mouse Brain (B) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, ELISA, Flow |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Concentration | 0.5 mg/ml |
| Immunogen | Peptide with sequence C-ELSKFVKDFSGN corresponding to internal region according to NP_004565.1, NP_536340.1, NP_536341.1, NP_001185642.1. |
| Epitope | C-ELSKFVKDFSGN |
| Specificity | This antibody is expected to recognize all reported isoforms (NP_004565.1; NP_536340.1; NP_536341.1, NP_001185642.1). Amino acid numbering in name refers to NP_004565.1 sequence. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | SEPTIN4 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
|
| Application Notes | WB: Approx 55kDa band observed in nuclear lysates of cell lines Jurkat and in LNCaP cell lysates, 50-55kDa in Human Cerebellum lysates, and approx. 50kDa in Mouse Brain lysates (calculated MW of 53.0kDa according to Human NP_536341.1 and 49.4kDa according to Mouse NP_001271323.1). |
|
| Publications |
|
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Immunogen affinity purified |
| Publication using NBP1-52096 | Applications | Species |
|---|---|---|
| Lhuillier P, Rode B, Escalier D et al. Absence of annulus in human asthenozoospermia: case report. Hum Reprod 2009-06-01 [PMID: 19221096] |
Secondary Antibodies |
Isotype Controls |
Research Areas for Septin-4 Antibody (NBP1-52096)Find related products by research area.
|
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | SEPTIN4 |
| Entrez | |
| Uniprot |
|
![Flow Cytometry: Septin-4 Antibody [NBP1-52096] - Analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/Septin-4-Antibody-Flow-Cytometry-NBP1-52096-img0003.jpg "Flow Cytometry: Septin-4 Antibody [NBP1-52096] - Analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.")
![Western Blot: Septin-4 Antibody [NBP1-52096] - (0.3ug/ml) staining of Jurkat nuclear (A) and LNCaP (B) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Septin-4-Antibody-Western-Blot-NBP1-52096-img0001.jpg "Western Blot: Septin-4 Antibody [NBP1-52096] - (0.3ug/ml) staining of Jurkat nuclear (A) and LNCaP (B) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.")
![Western Blot XIAP Antibody [Unconjugated]](https://images.novusbio.com/images2/XIAP_AF8221_Western_Blot_5911.jpg)
![Simple Western XIAP Antibody [Unconjugated]](https://images.novusbio.com/images2/XIAP_AF8221_Simple_Western_16640.jpg)
![Knockout Validated XIAP Antibody [Unconjugated]](https://images.novusbio.com/images2/XIAP_AF8221_Knockout_Validated_22488.jpg)
![N/A Haptoglobin [HRP]](https://images.novusbio.com/images2/Haptoglobin_DHAPG0_ELISA_461.jpg)
![N/A Haptoglobin [HRP]](https://images.novusbio.com/images2/DATA_Haptoglobin_DHAPG0_ELISA_709.jpg)
