| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33200 | |||
| Azide and BSA Free | NBP2-33200-1mg | |||
| IHC-Prediluted | NBP2-48225 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-2756SS | |||
| Azide and BSA Free | NBP2-80688 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48300 | |||
| Azide and BSA Free | NBP2-76425 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-89450-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44370 | |||
| Azide and BSA Free | NBP2-34394-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48197 | |||
| Azide and BSA Free | NBP2-34433-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47669-0.1mg | |||
| IHC-Prediluted | NBP2-48196 | |||
![Flow Cytometry: Cytokeratin, pan Antibody (AE-1/AE-3) [NBP2-29429] - An intracellular stain was performed on HeLa cells with pan Cytokeratin Antibody (AE1 + AE3) NBP2-33200AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647. Image from the AF647 version of this antibody.](http://images.novusbio.com/fullsize/nbp2-29429_mouse-monoclonal-cytokeratin-pan-antibody-ae-1-ae-3-imgenex-nbo-135a-20ug-17720249362612.jpg)
![Flow Cytometry: Cytokeratin, pan Antibody (AE-1/AE-3) [NBP2-29429] - An intracellular stain was performed on HeLa cells with pan Cytokeratin [AE1 + AE3] Antibody NBP2-33200AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488. Image from the AF488 version of this antibody.](http://images.novusbio.com/fullsize/nbp2-29429_mouse-monoclonal-cytokeratin-pan-antibody-ae-1-ae-3-imgenex-nbo-135a-20ug-17720241003217.jpg)
![Flow Cytometry: Cytokeratin, pan Antibody (AE-1/AE-3) [NBP2-29429] - An intracellular stain was performed on HeLa cells with pan Cytokeratin Antibody (AE1 + AE3) NBP2-33200PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to phycoerythrin. Image from the PE version of this antibody.](http://images.novusbio.com/fullsize/nbp2-29429_mouse-monoclonal-cytokeratin-pan-antibody-ae-1-ae-3-imgenex-nbo-135a-20ug-1772024944230.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 1 Antibody (LHK1) [NB100-2756] - IHC analysis of a formalin fixed paraffin-embedded (FFPE) human breast cancer using 5ug/ml conc. of Cytokeratin 1 antibody (clone LHK1) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) was performed using Aperio AT2 (Leica Biosystems). A membrane-cytoplasmic staining of Cytokeratin 1 was observed in the cancer cells with signal localized more to the cell membranes. Tumor cores showed a decreased staining and tumor stroma was mostly negative for Cytokeratin 1.](http://images.novusbio.com/fullsize/Cytokeratin-1-Antibody-LHK1-Immunohistochemistry-Paraffin-NB100-2756-img0009.jpg "Immunohistochemistry-Paraffin: Cytokeratin 1 Antibody (LHK1) [NB100-2756] - IHC analysis of a formalin fixed paraffin-embedded (FFPE) human breast cancer using 5ug/ml conc. of Cytokeratin 1 antibody (clone LHK1) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) was performed using Aperio AT2 (Leica Biosystems). A membrane-cytoplasmic staining of Cytokeratin 1 was observed in the cancer cells with signal localized more to the cell membranes. Tumor cores showed a decreased staining and tumor stroma was mostly negative for Cytokeratin 1.")
![Immunohistochemistry-Paraffin: Cytokeratin 1 Antibody (LHK1) [NB100-2756] - IHC analysis of a formalin fixed paraffin-embedded (FFPE) human breast cancer using 5ug/ml conc. of Cytokeratin 1 antibody (clone LHK1) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) was performed using Aperio AT2 (Leica Biosystems). A membrane-cytoplasmic staining of Cytokeratin 1 was observed in the cancer cells with signal localized more to the cell membranes. Tumor stroma was mostly negative for Cytokeratin 1. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/Cytokeratin-1-Antibody-LHK1-Immunohistochemistry-Paraffin-NB100-2756-img0008.jpg)
![Western Blot: Cytokeratin 1 Antibody (LHK1) [NB100-2756] - Analysis of Cytokeratin 1 in Caco-2.](http://images.novusbio.com/fullsize/Cytokeratin-1-Antibody-LHK1-Western-Blot-NB100-2756-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Cytokeratin 1 Antibody (LHK1) [NB100-2756] - Cytokeratin 1 antibody (LHK1) was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/Cytokeratin-1-Antibody-LHK1-Immunocytochemistry-Immunofluorescence-NB100-2756-img0005.jpg)
![Western Blot: Cytokeratin, pan Antibody (PCK-26) [NB120-6401] - Whole extract of HeLa cells was separated on SDS-PAGE and probed with Monoclonal Anti-Cytokeratin, pan Clone: PCK-26. WB was developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate. Lane 1: Antibody dilution 1:300. Lane 2: Antibody dilution 1:500. Lane 3: Antibody dilution 1:1000.](http://images.novusbio.com/fullsize/pan-Cytokeratin-Antibody-PCK-26-Western-Blot-NB120-6401-img0011.jpg "Western Blot: Cytokeratin, pan Antibody (PCK-26) [NB120-6401] - Whole extract of HeLa cells was separated on SDS-PAGE and probed with Monoclonal Anti-Cytokeratin, pan Clone: PCK-26. WB was developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate. Lane 1: Antibody dilution 1:300. Lane 2: Antibody dilution 1:500. Lane 3: Antibody dilution 1:1000.")
![Immunocytochemistry/Immunofluorescence: Cytokeratin, pan Antibody (PCK-26) [NB120-6401] - Human pancreas cancer cells. Antibody at 1:250 dilution. Overnight incubation. No permeabilization. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Cytokeratin-pan-Antibody-PCK-26-Immunocytochemistry-Immunofluorescence-NB120-6401-img0012.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin, pan Antibody (PCK-26) [NB120-6401] - Staining of FFPE human placenta sections with 1:300 Monoclonal Anti-Cytokeratin, pan Clone: PCK-26, followed by Goat Anti Mouse IgG (Fab)-FITC.](http://images.novusbio.com/fullsize/pan-Cytokeratin-Antibody-PCK-26-Immunohistochemistry-Paraffin-NB120-6401-img0006.jpg)
![Immunohistochemistry: Cytokeratin, pan Antibody (PCK-26) [NB120-6401] - Histological examination of the subcutaneous xenografts in nude mice with H&E staining (left panel) and IHC with anti-pan CK (right panel) at 400x magnification. Asterisks (*) indicate representative keratinization and keratin-pearl formation. Image collected and cropped by CiteAb from the following publication (mdpi.com/2072-6694/12/1/61), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Cytokeratin-pan-Antibody-PCK-26-Immunohistochemistry-NB120-6401-img0014.jpg)
![Immunohistochemistry: Cytokeratin, pan Antibody (PCK-26) [NB120-6401] - Histological examination of NHRI-HN1 tumors in nude mice and B6 mice with H&E staining (upper panels) at 400x and 1000x magnifications and IHC with anti-pan CK (middle panel) and EGFR (lower panel) at 400x magnification. Image collected and cropped by CiteAb from the following publication (mdpi.com/2072-6694/12/1/61), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Cytokeratin-pan-Antibody-PCK-26-Immunohistochemistry-NB120-6401-img0013.jpg)
![Immunocytochemistry/ Immunofluorescence: Cytokeratin, pan Antibody (PCK-26) [NB120-6401] - Detection of EMT & increased motility in NHRI-HN1 cells. (A) Summary of the most enriched pathways associated with tumorigenesis in syngeneic mice by comparing tumorigenic NHRI-HN1 cells with nontumorigenic cells, including M1-2, M2-3 & NHRI-HN2, using GSEA analysis. Blue indicates a negative normalized enrichment score (NES) & orange indicates a positive NES. (B) Morphology of M1-2 & NHRI-HN1 cells via phase-contrast microscopy at 100× magnification. (C) Relative adhesion activity in M1-2 & NHRI-HN1 cells, determined by normalizing the mean OD 490 nm value of NHRI-HN1 cells to that of M1-2 cells. (D) Cells stained with Alexa Fluor 488 phalloidin, anti-pan-CK & anti-EGFR antibodies at 400× magnification. (E) Immunoblot analysis of epithelial (E-cadherin, alpha -catenin & beta -catenin), mesenchymal (N-cadherin & Vimentin) proteins & EMT-related transcription factors, including Twist, Snail & Slug in M1-2 & NHRI-HN1 cells. Protein levels were normalized to an internal control, alpha -tubulin. Ratios were determined by dividing the normalized protein levels in NHRI-HN1 cells by that in M1-2 cells. (F) Representative images (left) & relative data (right) for migration activity of M1-2 & NHRI-HN1 cells. (G) Representative images (left) & relative data (right) for invasion activity of M1-2 & NHRI-HN1 cells at 200× magnification. The relative migration or invasion activity was determined by normalizing the mean number of cells that have migrated or invaded per field of NHRI-HN1 cells to that of M1-2 cells. Error bars represent SE; ** p < 0.01; *** p < 0.001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31878324), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb120-6401_mouse-monoclonal-cytokeratin-pan-antibody-pck-26-310202416212383.jpg)
![Flow Cytometry: Cytokeratin, pan Antibody (C-11) [NBP1-48348] - An intracellular stain was performed on HeLa cells with pan Cytokeratin antibody (C-11) NBP1-48348AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/Cytokeratin-pan-Antibody-C-11-Flow-Cytometry-NBP1-48348-img0015.jpg "Flow Cytometry: Cytokeratin, pan Antibody (C-11) [NBP1-48348] - An intracellular stain was performed on HeLa cells with pan Cytokeratin antibody (C-11) NBP1-48348AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.")
![Immunohistochemistry-Paraffin: Cytokeratin, pan Antibody (C-11) [NBP1-48348] - Staining of cytokeratin on guinea pig breast carcinoma.](http://images.novusbio.com/fullsize/Cytokeratin-pan-Antibody-C-11-Immunohistochemistry-Paraffin-NBP1-48348-img0002.jpg)
![Western Blot: Cytokeratin, pan Antibody (C-11) - BSA Free [NBP1-48348] - Analysis of human cytokeratins using mouse monoclonal antibody C-11 on lysates of HeLa cell line and MOLT-4 cell line (cytokeratin non-expressing cell line; negative control) under non-reducing and reducing conditions. Nitrocellulose membrane was probed with 2 ug/ml of mouse monoclonal antibody anti-cytokeratins followed by IRDye800-conjugated anti-mouse secondary antibody. Specific bands were detected for cytokeratins at approximately 45-55 kDa.](http://images.novusbio.com/fullsize/Cytokeratin-pan-Antibody-C-11-BSA-Free-Western-Blot-NBP1-48348-img0018.jpg)
![Immunocytochemistry/Immunofluorescence: Cytokeratin, pan Antibody (C-11) - BSA Free [NBP1-48348] - Staining of cytokeratins in Hep-2 cells using pan-cytokeratin antibody C-11 (diluted 1:400), detected with GAM IgG-Alexa Fluor®488 (diluted 1:200; green).](http://images.novusbio.com/fullsize/Cytokeratin-pan-Antibody-C-11-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP1-48348-img0017.jpg)
![Flow (Intracellular): Cytokeratin, pan Antibody (C-11) - BSA Free [NBP1-48348] - Intracellular staining pattern of human peripheral whole blood spiked with MCF-7 cells stained using anti-Cytokeratins (C-11) purified antibody (concentration in sample 3 ug/ml, GAM APC).](http://images.novusbio.com/fullsize/Cytokeratin-pan-Antibody-C-11-BSA-Free-Flow-Intracellular-NBP1-48348-img0019.jpg)
![Immunocytochemistry/Immunofluorescence: Cytokeratin, pan Antibody (C-11) [NBP1-48348] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-pan Cytokeratin (C-11) conjugated to Alexa Fluor 488 [NBP2-33134AF488] at 10 ug/mL for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image from the Alexa Fluor 488 version of this antibody.](http://images.novusbio.com/fullsize/Cytokeratin-pan-Antibody-C-11-Immunocytochemistry-Immunofluorescence-NBP1-48348-img0014.jpg)
![Flow (Intracellular): Cytokeratin, pan Antibody (C-11) [NBP1-48348] - An intracellular stain was performed on HeLa cells with pan Cytokeratin (C-11) antibody NBP1-48348 (blue) and a matched isotype control NBP2-27287 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes, followed by mouse F(ab)2 IgG (H+L) PE-conjugated secondary antibody (F0102B, R&D Systems).](http://images.novusbio.com/fullsize/Cytokeratin-pan-Antibody-C-11-Flow-Intracellular-NBP1-48348-img0005.jpg)
![Flow Cytometry: Cytokeratin, pan Antibody (C-11) [NBP1-48348] - Intracellular flow cytometry analysis of cytokeratin expression in HT-29 human Caucasian colon adenocarcinoma cell line using anti-cytokeratin antibody (C-11) PE. Overlay with Isotype mouse IgG1 control.](http://images.novusbio.com/fullsize/Cytokeratin-pan-Antibody-C-11-Flow-Cytometry-NBP1-48348-img0007.jpg)
![Flow (Intracellular): Cytokeratin, pan Antibody (C-11) [NBP1-48348] - An intracellular stain was performed on HeLa cells with pan Cytokeratin antibody (C-11) NBP1-48348AF647 (blue) and a matched isotype control NBP2-27287AF647 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/Cytokeratin-pan-Antibody-C-11-Flow-Intracellular-NBP1-48348-img0010.jpg)
![Flow Cytometry: Cytokeratin, pan Antibody (C-11) [NBP1-48348] - An intracellular stain was performed on A549 cells with pan Cytokeratin Antibody (C-11) NBP1-48348F488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/Cytokeratin-pan-Antibody-C-11-Flow-Cytometry-NBP1-48348-img0012.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin, pan Antibody [NB600-579] - Human skin stained with Cytokeratin antibody.](http://images.novusbio.com/fullsize/pan-Cytokeratin-Antibody-Immunohistochemistry-Paraffin-NB600-579-img0001.jpg "Immunohistochemistry-Paraffin: Cytokeratin, pan Antibody [NB600-579] - Human skin stained with Cytokeratin antibody.")
![Immunocytochemistry/Immunofluorescence: Cytokeratin, pan Antibody (PAN-CK (Cocktail)) [NBP2-44368] - Confocal immunofluorescent analysis of HeLa cells using Cytokeratin, panMouse Monoclonal Antibody (PAN-CK) followed by goat anti-mouse IgG-CF488 (cyan). Nuclei are counterstained with NucSpot (red).](http://images.novusbio.com/fullsize/Cytokeratin-pan-Antibody-PAN-CK-Cocktail-Immunocytochemistry-Immunofluorescence-NBP2-44368-img0003.jpg "Immunocytochemistry/Immunofluorescence: Cytokeratin, pan Antibody (PAN-CK (Cocktail)) [NBP2-44368] - Confocal immunofluorescent analysis of HeLa cells using Cytokeratin, panMouse Monoclonal Antibody (PAN-CK) followed by goat anti-mouse IgG-CF488 (cyan). Nuclei are counterstained with NucSpot (red).")
![Immunohistochemistry-Paraffin: Cytokeratin, pan Antibody (PAN-CK (Cocktail)) [NBP2-44368] - Formalin-fixed, paraffin-embedded human squamous cell carcinoma stained with Cytokeratin, pan Mouse Monoclonal Antibody (PAN-CK).](http://images.novusbio.com/fullsize/Cytokeratin-pan-Antibody-PAN-CK-Cocktail-Immunohistochemistry-Paraffin-NBP2-44368-img0004.jpg)
![Flow Cytometry: Cytokeratin, pan Antibody (PAN-CK (Cocktail)) [NBP2-44368] - Flow Cytometric Analysis of human HeLa cells. Cytokeratin, pan Antibody (PAN-CK (Cocktail)). using followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).](http://images.novusbio.com/fullsize/Cytokeratin-pan-Antibody-PAN-CK-Cocktail-Flow-Cytometry-NBP2-44368-img0002.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin, pan Antibody (PAN-CK (Cocktail)) [NBP2-44368] - Human Skin stained with Cytokeratin, pan Monoclonal Antibody cocktail (PAN-CK).](http://images.novusbio.com/fullsize/Cytokeratin-pan-Antibody-PAN-CK-Cocktail-Immunohistochemistry-Paraffin-NBP2-44368-img0001.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin, pan Antibody (PAN-CK (Cocktail)) [NBP2-44368] - Human breast cancer tissue stained for panCK (white) and counterstained with DAPI (blue). The Alexa Fluor 750 conjugate of this antibody was used (NBP2-76425AF750). Image from verified customer review.](http://images.novusbio.com/fullsize/nbp2-44368_mouse-monoclonal-cytokeratin-pan-antibody-pan-ck-cocktail-1772024100325.jpg)
![Immunohistochemistry-Frozen: Cytokeratin, pan Antibody (PAN-CK (Cocktail)) [NBP2-44368] - Human breast cancer tissue stained for panCK (purple) and counterstained with DAPI (blue). The Alexa Fluor 750 conjugate of this antibody was used. IHC-Fr image submitted by a verified customer review.](http://images.novusbio.com/fullsize/nbp2-44368_mouse-monoclonal-cytokeratin-pan-antibody-pan-ck-cocktail-1772024959414.jpg)
![Western Blot: Cytokeratin 1 Antibody (10W8J6) [NBP3-16883] - Western blot analysis of extracts of various cell lines, using Cytokeratin 1 (KRT1) Rabbit mAb (NBP3-16883) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Cytokeratin-1-Antibody-10W8J6-Western-Blot-NBP3-16883-img0007.jpg "Western Blot: Cytokeratin 1 Antibody (10W8J6) [NBP3-16883] - Western blot analysis of extracts of various cell lines, using Cytokeratin 1 (KRT1) Rabbit mAb (NBP3-16883) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunohistochemistry: Cytokeratin 1 Antibody (10W8J6) [NBP3-16883] - Immunofluorescence analysis of Mouse tail and ear using Cytokeratin 1 (KRT1) Rabbit mAb (NBP3-16883) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Cytokeratin-1-Antibody-10W8J6-Immunohistochemistry-NBP3-16883-img0006.jpg)
![Western Blot: Cytokeratin 1 Antibody (10W8J6) [NBP3-16883] - Western blot analysis of extracts of A375 cells, using Cytokeratin 1 (KRT1) Rabbit mAb (NBP3-16883) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/Cytokeratin-1-Antibody-10W8J6-Western-Blot-NBP3-16883-img0008.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 1 Antibody (10W8J6) [NBP3-16883] - Immunohistochemistry of paraffin-embedded rat skin using Cytokeratin 1 (KRT1) Rabbit mAb (NBP3-16883) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Cytokeratin-1-Antibody-10W8J6-Immunohistochemistry-Paraffin-NBP3-16883-img0001.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 1 Antibody (10W8J6) [NBP3-16883] - Immunohistochemistry of paraffin-embedded human esophageal cancer using Cytokeratin 1 (KRT1) Rabbit mAb (NBP3-16883) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Cytokeratin-1-Antibody-10W8J6-Immunohistochemistry-Paraffin-NBP3-16883-img0002.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 1 Antibody (10W8J6) [NBP3-16883] - Immunohistochemistry of paraffin-embedded mouse skin using Cytokeratin 1 (KRT1) Rabbit mAb (NBP3-16883) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Cytokeratin-1-Antibody-10W8J6-Immunohistochemistry-Paraffin-NBP3-16883-img0003.jpg)
![Immunohistochemistry: Cytokeratin 1 Antibody (10W8J6) [NBP3-16883] - Immunofluorescence analysis of rat skin using Cytokeratin 1 (KRT1) Rabbit mAb (NBP3-16883) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Cytokeratin-1-Antibody-10W8J6-Immunohistochemistry-NBP3-16883-img0004.jpg)
![Immunohistochemistry: Cytokeratin 1 Antibody (10W8J6) [NBP3-16883] - Immunofluorescence analysis of human skin using Cytokeratin 1 (KRT1) Rabbit mAb (NBP3-16883) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Cytokeratin-1-Antibody-10W8J6-Immunohistochemistry-NBP3-16883-img0005.jpg)
![Western Blot: Cytokeratin 1 Antibody (3C10F7) [NB110-89450] - Analysis using CK1 mouse mAb against truncated CK1 recombinant protein.](http://images.novusbio.com/fullsize/Cytokeratin-1-Antibody-3C10F7-Western-Blot-NB110-89450-img0004.jpg "Western Blot: Cytokeratin 1 Antibody (3C10F7) [NB110-89450] - Analysis using CK1 mouse mAb against truncated CK1 recombinant protein.")
![Immunocytochemistry/Immunofluorescence: Cytokeratin 1 Antibody (3C10F7) [NB110-89450] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Cytokeratin 1 (3C10F7) at 5 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Cytokeratin-1-Antibody-3C10F7-Immunocytochemistry-Immunofluorescence-NB110-89450-img0006.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 1 Antibody (3C10F7) [NB110-89450] - Analysis of human liver tissue (A), colon carcinoma (B), lung carcinoma (C) and esophagus tissue (D), showing membrane localization using CK1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Cytokeratin-1-Antibody-3C10F7-Immunohistochemistry-Paraffin-NB110-89450-img0005.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin, pan Antibody (SPM115 + SPM116) [NBP2-34347] - FFPE human breast carcinoma stained with pan cytokeratin monoclonal antibody cocktial (SPM115+SPM116).](http://images.novusbio.com/fullsize/Cytokeratin-pan-Antibody-SPM115-+-SPM116-Immunohistochemistry-Paraffin-NBP2-34347-img0001.jpg "Immunohistochemistry-Paraffin: Cytokeratin, pan Antibody (SPM115 + SPM116) [NBP2-34347] - FFPE human breast carcinoma stained with pan cytokeratin monoclonal antibody cocktial (SPM115+SPM116).")
![Flow Cytometry: Cytokeratin, pan Antibody (SPM115 + SPM116) [NBP2-34347] - Flow Cytometric analysis of human Cytokeratin, pans on HeLa cells. Black: cells alone; Green: Isotype Control; Red: PE-labeled Cytokeratin, pan Monoclonal Antibody (SPM115 + SPM116).](http://images.novusbio.com/fullsize/Cytokeratin-pan-Antibody-SPM115-+-SPM116-Flow-Cytometry-NBP2-34347-img0005.jpg)
![Flow (Intracellular): Cytokeratin, pan Antibody (SPM115 + SPM116) [NBP2-34347] - An intracellular stain was performed on HeLa cells with Cytokeratin, pan Antibody (SPM115 + SPM116) NBP2-34394APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to allophycocyanin. Image using the Allophycocyanin format of this antibody.](http://images.novusbio.com/fullsize/nbp2-34347_mouse-monoclonal-cytokeratin-pan-antibody-spm115-spm116-17720241003222.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin, pan Antibody (SPM583) [NBP2-34386] - Formalin-fixed, paraffin-embedded colon tissue (10X) stained with Cytokeratin pan antibody (SPM583).](http://images.novusbio.com/fullsize/Cytokeratin-pan-Antibody-SPM583-Immunohistochemistry-Paraffin-NBP2-34386-img0004.jpg "Immunohistochemistry-Paraffin: Cytokeratin, pan Antibody (SPM583) [NBP2-34386] - Formalin-fixed, paraffin-embedded colon tissue (10X) stained with Cytokeratin pan antibody (SPM583).")
![Flow Cytometry: Cytokeratin, pan Antibody (SPM583) [NBP2-34386] - Analysis of Alexa Fluor (R) 488 conjugate of NBP2-34386. An intracellular stain was performed on HeLa cells with pan Cytokeratin (SMP583) antibody NBP2-34433AF488 (blue) and a matched isotype control NBP2-27287AF488 (orange). Cells were fixed with 4% PFA](http://images.novusbio.com/fullsize/Cytokeratin-pan-Antibody-SPM583-Flow-Cytometry-NBP2-34386-img0001.jpg)
![Western Blot: Cytokeratin 1 Antibody (SN72-08) [NBP2-67681] - Western blot analysis of Cytokeratin 1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat](http://images.novusbio.com/fullsize/Cytokeratin-1-Antibody-SN72-08-Western-Blot-NBP2-67681-img0003.jpg "Western Blot: Cytokeratin 1 Antibody (SN72-08) [NBP2-67681] - Western blot analysis of Cytokeratin 1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat")
![Immunohistochemistry-Paraffin: Cytokeratin 1 Antibody (SN72-08) [NBP2-67681] - Analysis of paraffin-embedded rat skin tissue with Rabbit anti-Cytokeratin 1 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Cytokeratin-1-Antibody-SN72-08-Immunohistochemistry-Paraffin-NBP2-67681-img0006.jpg)
![Western Blot: Cytokeratin 1 Antibody (SN72-08) [NBP2-67681] - Analysis of Cytokeratin 1 on different lysates using anti-Cytokeratin 1 antibody at 1/1,000 dilution. Positive control: Lane 1: 293T Lane 2: Hela](http://images.novusbio.com/fullsize/Cytokeratin-1-Antibody-SN72-08-Western-Blot-NBP2-67681-img0002.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 1 Antibody (SN72-08) [NBP2-67681] - Analysis of paraffin-embedded mouse skin tissue using anti-Cytokeratin 1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Cytokeratin-1-Antibody-SN72-08-Immunohistochemistry-Paraffin-NBP2-67681-img0001.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 1 Antibody (SN72-08) [NBP2-67681] - Immunofluorescence analysis of paraffin-embedded human skin tissue labeling Cytokeratin 1 (washed with PBS. And then probed with the primary antibodies Cytokeratin 1 (red) at 1/200 dilution and Vimentin (green) at 1/200 dilution at +4 overnight, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor(TM) 594) and Goat Anti-Mouse IgG H&L (iFluor(TM) 488) were used as the secondary antibodies at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Cytokeratin-1-Antibody-SN72-08-Immunohistochemistry-Paraffin-NBP2-67681-img0004.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 1 Antibody (SN72-08) [NBP2-67681] - Analysis of paraffin-embedded human skin tissue using anti-Cytokeratin 1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Cytokeratin-1-Antibody-SN72-08-Immunohistochemistry-Paraffin-NBP2-67681-img0005.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin, pan Antibody (KRT/457) [NBP2-45309] - Rat Oviduct stained with Multi Cytokeratin Monoclonal Antibody (KRT/457).](http://images.novusbio.com/fullsize/Cytokeratin-pan-Antibody-KRT-457-Immunohistochemistry-Paraffin-NBP2-45309-img0003.jpg "Immunohistochemistry-Paraffin: Cytokeratin, pan Antibody (KRT/457) [NBP2-45309] - Rat Oviduct stained with Multi Cytokeratin Monoclonal Antibody (KRT/457).")
![Immunohistochemistry-Paraffin: Cytokeratin, pan Antibody (KRT/457) [NBP2-45309] - Human Bladder Carcinoma stained with Multi Cytokeratin Monoclonal Antibody (KRT/457).](http://images.novusbio.com/fullsize/Cytokeratin-pan-Antibody-KRT-457-Immunohistochemistry-Paraffin-NBP2-45309-img0001.jpg)