Could you advise on the best way to prepare a cell lysate for use with your beta-glucosidase assay kit (KA1611)?

Name: Glucosylceramidase/GBA Assay Kit (Colorimetric)
Brand: Novus Biologicals
SKU: KA1611
Price: 849 USD

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This particular product is made by a Taiwanese company called Abnova; we distribute their products in addition to selling our own. Here is a link to their protocol for KA1611. We suggest to prepare lysate in 50 mM phosphate (pH 7.0) buffer or in any other suitable enzyme buffer. However, the following chemicals are known to affect the enzyme activity and should be avoided: SH-containing reagents (e.g. dithiothreitol, 2-mercaptoethanol, glutathione), Ca2+, Cu2+, Fe3+/Fe2+, Hg2+, Mg2+, Ni2+, Zn2+, SDS, EDTA and Tris.

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	All-NASpecies Glossary
Applications	Func, Enzyme Activity, NULL
Suitable Sample Type	Biological
Standard Curve Range	2 to 250 U/L
Sensitivity	2 U/L

Description	beta-Glucosidase Assay Kit is a colorimetric kinetic determination of beta-Glucosidase activity.
Standard Curve Range	2 to 250 U/L
Sensitivity	2 U/L
Assay Type	Colorimetric
Sample Volume	20 uL
Kit Type	Assay Kit (Colorimetric)
Gene	GBA1

Publications using KA1611	Applications	Species
Hideaki M, Junko I, Noriko M et al. Cytosolic dsDNA of mitochondrial origin induces cytotoxicity and neurodegeneration in cellular and zebrafish models of Parkinson's disease. Nat Commun. 2021-05-25 [PMID: 34035300]
Riessland M, Kolisnyk B, Kim TW et al. Loss of SATB1 Induces p21-Dependent Cellular Senescence in Post-mitotic Dopaminergic Neurons Cell Stem Cell 2019-09-10 [PMID: 31543366] (Human, Mouse)		Human, Mouse
Abul S, Dhanushkodi N, Ardah M et al. Silencing of Glucocerebrosidase Gene in Drosophila Enhances the Aggregation of Parkinson's Disease Associated ?-Synuclein Mutant A53T and Affects Locomotor Activity. Front Neurosci. 2018-02-16 [PMID: 29503608]
Ibrahim E, Jones KD, Taylor KE et al. Molecular and biochemical characterization of recombinant cel12B, cel8C, and peh28 overexpressed in Escherichia coli and their potential in biofuel production. Biotechnol Biofuels 2017-02-27 [PMID: 28413443]
Attia RT, Tolba MF, Trivedi R et al. The chemomodulatory effects of glufosfamide on docetaxel cytotoxicity in prostate cancer cells Peer J 2016-06-29 [PMID: 27413637]

Glucosylceramidase/GBA Assay Kit (Colorimetric)

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