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![Western Blot: DDX3X Overexpression Lysate [NBL1-09795] - Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for DDX3.](http://images.novusbio.com/fullsize/DDX3-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-09795-img0003.jpg "Western Blot: DDX3X Overexpression Lysate [NBL1-09795] - Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for DDX3.")
![Western Blot: DDX3 Antibody (3B9E3) [NBP2-52457] - Analysis of DDX3X, DDX5 protein expression levels by Western blot in DTMUV-infected and control baby hamster kidney cell line BHK-21. From a verified customer review.](http://images.novusbio.com/fullsize/DDX3-Antibody-3B9E3-Western-Blot-NBP2-52457-img0010.jpg "Western Blot: DDX3 Antibody (3B9E3) [NBP2-52457] - Analysis of DDX3X, DDX5 protein expression levels by Western blot in DTMUV-infected and control baby hamster kidney cell line BHK-21. From a verified customer review.")
![Immunohistochemistry: DDX3 Antibody (3B9E3) [NBP2-52457] - Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using DDX3X mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/DDX3-Antibody-3B9E3-Immunohistochemistry-NBP2-52457-img0006.jpg)
![Flow Cytometry: DDX3 Antibody (3B9E3) [NBP2-52457] - Analysis of HepG2 cells using DDX3X mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/DDX3-Antibody-3B9E3-Flow-Cytometry-NBP2-52457-img0002.jpg)
![Western Blot: DDX3 Antibody (3B9E3) [NBP2-52457] - Analysis using DDX3X mouse mAb against Hela (1), NIH3T3 (2), C6 (3), COS7 (4), A431 (5), and HEK293 (6) cell lysate.](http://images.novusbio.com/fullsize/DDX3-Antibody-3B9E3-Western-Blot-NBP2-52457-img0007.jpg)
![Western Blot: DDX3 Antibody (3B9E3) [NBP2-52457] - Analysis using DDX3X mAb against HEK293 (1) and DDX3X (AA: 518-661)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/DDX3-Antibody-3B9E3-Western-Blot-NBP2-52457-img0009.jpg)
![Western Blot: DDX3 Antibody (3B9E3) [NBP2-52457] - Analysis using DDX3X mAb against human DDX3X (AA: 518-661) recombinant protein. (Expected MW is 41.4 kDa)](http://images.novusbio.com/fullsize/DDX3-Antibody-3B9E3-Western-Blot-NBP2-52457-img0008.jpg)
![Immunohistochemistry: DDX3 Antibody (3B9E3) [NBP2-52457] - Analysis of paraffin-embedded cervical cancer tissues using DDX3X mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/DDX3-Antibody-3B9E3-Immunohistochemistry-NBP2-52457-img0005.jpg)
![Flow Cytometry: DDX3 Antibody (3B9E3) [NBP2-52457] - Analysis of Hela cells using DDX3X mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/DDX3-Antibody-3B9E3-Flow-Cytometry-NBP2-52457-img0001.jpg)
![Immunofluorescence: DDX3 Antibody (3B9E3) [NBP2-52457] - Analysis of Hela cells using DDX3X mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/DDX3-Antibody-3B9E3-Immunofluorescence-NBP2-52457-img0003.jpg)
![Immunofluorescence: DDX3 Antibody (3B9E3) [NBP2-52457] - Immunofluorescence analysis of Hela cells using DDX3X mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/DDX3-Antibody-3B9E3-Immunofluorescence-NBP2-52457-img0004.jpg)
![Western Blot: DDX3 Antibody [NBP1-85291] - Analysis: NBP1-85291 (A) shows similar pattern to independent antibody NBP1-85290 (B)](http://images.novusbio.com/fullsize/DDX3-Antibody-Western-Blot-NBP1-85291-img0027.jpg "<b>Independent Antibodies Validation. </b>Western Blot: DDX3 Antibody [NBP1-85291] - Analysis: NBP1-85291 (A) shows similar pattern to independent antibody NBP1-85290 (B)")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: DDX3 Antibody [NBP1-85291] - Staining of human cell line U-251 MG shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/DDX3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85291-img0019.jpg)
![Immunohistochemistry: DDX3 Antibody [NBP1-85291] - Staining of mouse hippocampus shows moderate positivity in a subset of neurons in the brain.](http://images.novusbio.com/fullsize/DDX3-Antibody-Immunohistochemistry-Paraffin-NBP1-85291-img0026.jpg)
![Western Blot: DDX3 Antibody [NBP1-85291] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/DDX3-Antibody-Western-Blot-NBP1-85291-img0018.jpg)
![Immunohistochemistry: DDX3 Antibody [NBP1-85291] - Staining of mouse medulla shows strong cytoplasmic immunoreactivity in neurons of the facial nucleus.](http://images.novusbio.com/fullsize/DDX3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85291-img0007.jpg)
![Immunohistochemistry: DDX3 Antibody [NBP1-85291] - Staining of mouse thalamus shows positivity in neurons of the anterodorsal thalamic nucleus.](http://images.novusbio.com/fullsize/DDX3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85291-img0009.jpg)
![Immunohistochemistry: DDX3 Antibody [NBP1-85291] - Staining of mouse midbrain shows neuronal positivity in the red nucleus.](http://images.novusbio.com/fullsize/DDX3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85291-img0012.jpg)
![Immunohistochemistry-Paraffin: DDX3 Antibody [NBP1-85291] - Staining of human cerebellum shows moderate nuclear immunoreactivity in neurons.](http://images.novusbio.com/fullsize/DDX3-Antibody-Immunohistochemistry-Paraffin-NBP1-85291-img0014.jpg)
![Immunohistochemistry-Paraffin: DDX3 Antibody [NBP1-85291] - Staining of human cerebral cortex shows moderate nuclear positivity in neurons.](http://images.novusbio.com/fullsize/DDX3-Antibody-Immunohistochemistry-Paraffin-NBP1-85291-img0020.jpg)
![Immunohistochemistry-Paraffin: DDX3 Antibody [NBP1-85291] - Staining of human tonsil shows moderate cytoplasmic positivity in germinal center cells.](http://images.novusbio.com/fullsize/DDX3-Antibody-Immunohistochemistry-Paraffin-NBP1-85291-img0021.jpg)
![Immunohistochemistry-Paraffin: DDX3 Antibody [NBP1-85291] - Staining of human hippocampus shows moderate positivity in neurons.](http://images.novusbio.com/fullsize/DDX3-Antibody-Immunohistochemistry-Paraffin-NBP1-85291-img0022.jpg)
![Immunohistochemistry-Paraffin: DDX3 Antibody [NBP1-85291] - Staining of human Fallopian tube shows weak cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/DDX3-Antibody-Immunohistochemistry-Paraffin-NBP1-85291-img0023.jpg)
![Immunohistochemistry-Paraffin: DDX3 Antibody [NBP1-85291] - Staining of human cerebellum shows moderate nuclear positivity in neurons.](http://images.novusbio.com/fullsize/DDX3-Antibody-Immunohistochemistry-Paraffin-NBP1-85291-img0024.jpg)
![Immunohistochemistry: DDX3 Antibody [NBP1-85291] - Staining of mouse cerebral cortex shows strong positivity in neurons in the parietal association cortex.](http://images.novusbio.com/fullsize/DDX3-Antibody-Immunohistochemistry-Paraffin-NBP1-85291-img0025.jpg)
![Western Blot: DDX3 Antibody [NB200-194] - Detection of human and mouse DDX3 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DDX3 antibody NB200-194 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/DDX3-Antibody-Western-Blot-NB200-194-img0008.jpg "Western Blot: DDX3 Antibody [NB200-194] - Detection of human and mouse DDX3 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DDX3 antibody NB200-194 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.")
![Immunohistochemistry: DDX3 Antibody [NB200-194] - Detection of human DDX3 by immunohistochemistry. Sample: FFPE section of human lung cancer. Antibody: Affinity purified rabbit anti-DDX3 (NB200-194 lot 2) used at a dilution of 1:200 (1ug/ml). Detection: DAB.](http://images.novusbio.com/fullsize/DDX3-Antibody-Immunohistochemistry-NB200-194-img0010.jpg)
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: DDX3 Antibody [NB200-194] - Detection of DDX3 by Western Blot and Immunoprecipitation. Samples: A. Whole cell lysate from HeLa (5, 15 and 50 mcg) and Ramos (50 mcg) cells. B. Whole cell lysate (1 mg/IP; 1/4 of IP loaded/lane) from HeLa cells. Antibodies: NB 200-194 used at 0.04 mcg/ml (A) and 0.1 mcg/ml (B) for Western Blot and at 3 mcg/mg lysate for IP (B). DDX3 was also immunoprecipitated using NB 200-195 and NB 200-196 at 3 mcg/mg lysate. Detection: Chemiluminescence with an exposure time of 10 seconds (A and B).](http://images.novusbio.com/fullsize/DDX3-Antibody-Western-Blot-NB200-194-img0005.jpg)
![Immunohistochemistry: DDX3 Antibody [NB200-194] - Sample: FFPE section of human lung cancer pleural effusion (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti-DDX3 used at a dilution of 1:200 (1ug/ml). Detection: Red fluorescence (A120-601D4) and DAB.](http://images.novusbio.com/fullsize/DDX3-Antibody-Immunohistochemistry-NB200-194-img0006.jpg)
![Immunohistochemistry: DDX3 Antibody [NB200-194] - Detection of mouse DDX3 by immunohistochemistry. Sample: FFPE section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti-DDX3 (NB200-194 lot 2) used at a dilution of 1:200 (1ug/ml). Detection: DAB.](http://images.novusbio.com/fullsize/DDX3-Antibody-Immunohistochemistry-NB200-194-img0009.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: DDX3 Antibody [NB200-194] - Detection of human DDX3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DDX3 antibody NB200-194 (lot NB200-194-2) used for IP at 6 ug per reaction. DDX3 was also immunoprecipitated by a previous lot of this antibody (lot NB200-194-1) and rabbit anti-DDX3 antibody NB200-196. For blotting immunoprecipitated DDX3, NB200-194 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 seconds.](http://images.novusbio.com/fullsize/DDX3-Antibody-Immunoprecipitation-NB200-194-img0007.jpg)
![Western Blot: DDX3 Antibody [NBP2-92850] - nalysis of extracts of various cell lines, using DDX3Y at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.](http://images.novusbio.com/fullsize/DDX3-Antibody-Western-Blot-NBP2-92850-img0008.jpg "Western Blot: DDX3 Antibody [NBP2-92850] - nalysis of extracts of various cell lines, using DDX3Y at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.")
![Immunocytochemistry/Immunofluorescence: DDX3 Antibody [NBP2-92850] - Analysis of U-2 OS cells using DDX3Y . Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/DDX3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92850-img0003.jpg)
![Immunohistochemistry-Paraffin: DDX3 Antibody [NBP2-92850] - Rat testis using DDX3Y antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/DDX3-Antibody-Immunohistochemistry-Paraffin-NBP2-92850-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: DDX3 Antibody [NBP2-92850] - Analysis of C6 cells using DDX3Y . Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/DDX3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92850-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: DDX3 Antibody [NBP2-92850] - Analysis of L929 cells using DDX3Y . Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/DDX3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92850-img0002.jpg)
![Immunohistochemistry-Paraffin: DDX3 Antibody [NBP2-92850] - Rat brain using DDX3Y antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/DDX3-Antibody-Immunohistochemistry-Paraffin-NBP2-92850-img0004.jpg)
![Immunohistochemistry-Paraffin: DDX3 Antibody [NBP2-92850] - Mouse heart using DDX3Y antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/DDX3-Antibody-Immunohistochemistry-Paraffin-NBP2-92850-img0005.jpg)
![Immunohistochemistry-Paraffin: DDX3 Antibody [NBP2-92850] - Mouse brain using DDX3Y antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/DDX3-Antibody-Immunohistochemistry-Paraffin-NBP2-92850-img0006.jpg)
![Western Blot: DDX3 Antibody [NBP1-32780] - A. 30 ug Jurkat B. 30 ug Raji C. 30 ug NCI-H929 7.5 % SDS-PAGE DDX3 antibody dilution: 1:10000](http://images.novusbio.com/fullsize/DDX3Y-Antibody-Western-Blot-NBP1-32780-img0013.jpg "Western Blot: DDX3 Antibody [NBP1-32780] - A. 30 ug Jurkat B. 30 ug Raji C. 30 ug NCI-H929 7.5 % SDS-PAGE DDX3 antibody dilution: 1:10000")
![Immunocytochemistry/Immunofluorescence: DDX3 Antibody [NBP1-32780] - Analysis of paraformaldehyde-fixed HeLa, using DDX3Y antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/DDX3Y-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32780-img0012.jpg)
![Western Blot: DDX3 Antibody [NBP1-32780] - Sample (50 ug of whole cell lysate) A: Mouse brain 7. 5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/DDX3Y-Antibody-Western-Blot-NBP1-32780-img0009.jpg)
![Western Blot: DDX3 Antibody [NBP1-32780] - Sample (30 ug of whole cell lysate)A: H1299B: Hep G2 7. 5% SDS PAGE, antibody diluted at 1:10000.](http://images.novusbio.com/fullsize/DDX3Y-Antibody-Western-Blot-NBP1-32780-img0011.jpg)
![Western Blot: DDX3 Antibody [NBP1-32780] - DDX3 antibody detects DDX3 protein by Western blot analysis.A. 50 ug rat testis lysate/extract7.5 % SDS-PAGEDDX3 antibody (NBP1-32780) dilution: 1:2000](http://images.novusbio.com/fullsize/nbp1-32780_rabbit-polyclonal-ddx3-antibody-910202420122062.jpg)
![Western Blot: DDX3 Antibody [NBP1-32780] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DDX3 antibody (NBP1-32780) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32780_rabbit-polyclonal-ddx3-antibody-91020242013404.jpg)
![Western Blot: DDX3 Antibody [NBP1-32780] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DDX3 antibody (NBP1-32780) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32780_rabbit-polyclonal-ddx3-antibody-91020242075947.jpg)
![Immunocytochemistry/ Immunofluorescence: DDX3 Antibody [NBP2-16133] - DDX3 antibody [N3C2], Internal detects DDX3 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: DDX3 stained by DDX3 antibody [N3C2], Internal (NBP2-16133) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar= 10 um.](http://images.novusbio.com/fullsize/nbp2-16133_rabbit-polyclonal-ddx3-antibody-91020242041258.jpg "Immunocytochemistry/ Immunofluorescence: DDX3 Antibody [NBP2-16133] - DDX3 antibody [N3C2], Internal detects DDX3 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: DDX3 stained by DDX3 antibody [N3C2], Internal (NBP2-16133) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar= 10 um.")
![Western Blot: DDX3 Antibody [NBP2-16133] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DDX3 antibody [N3C2], Internal (NBP2-16133) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-16133_rabbit-polyclonal-ddx3-antibody-91020242021912.jpg)
![Western Blot: DDX3 Antibody [NBP2-16133] - Sample (20 ug of whole cell lysate) A: human ESC 7. 5% SDS PAGE gel, diluted at 1:2000.](http://images.novusbio.com/fullsize/DDX3-Antibody-Western-Blot-NBP2-16133-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: DDX3 Antibody [NBP2-16133] - Analysis of paraformaldehyde-fixed Human ESC, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/DDX3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16133-img0005.jpg)
![Immunohistochemistry-Paraffin: DDX3 Antibody [NBP2-16133] - DDX3 antibody [N3C2], Internal detects DDX3 protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human breast carcinoma.DDX3 stained by DDX3 antibody [N3C2], Internal (NBP2-16133) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-16133_rabbit-polyclonal-ddx3-antibody-910202419593518.jpg)
![Western Blot: DDX3 Antibody [NBP2-16133] - U87-MG whole cell and cytoplasm extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DDX3 antibody [N3C2], Internal (NBP2-16133) diluted at 1:2000.](http://images.novusbio.com/fullsize/nbp2-16133_rabbit-polyclonal-ddx3-antibody-91020242091436.jpg)
![Western Blot: DDX3 Antibody (11F11D10) [NBP2-14848] - Analysis of human DDX3 using A) a partial recombinant protein (amino acids 24-203) and B) 293 lysate probed with DDX3 antibody at 2 ug/ml. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/DDX3-Antibody-11F11D10-Western-Blot-NBP2-14848-img0003.jpg "Western Blot: DDX3 Antibody (11F11D10) [NBP2-14848] - Analysis of human DDX3 using A) a partial recombinant protein (amino acids 24-203) and B) 293 lysate probed with DDX3 antibody at 2 ug/ml. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Flow Cytometry: DDX3 Antibody (11F11D10) [NBP2-14848] - Analysis of DDX3 in human MCF-7 and HeLa cells using DDX3 antibody at 1 ug/10^6 cells.](http://images.novusbio.com/fullsize/DDX3-Antibody-11F11D10-Flow-Cytometry-NBP2-14848-img0002.jpg)
![Western Blot: DDX3 Antibody (2D7) [H00008653-M01] - DDX3Y monoclonal antibody (M01), clone 2D7. Analysis of DDX3Y expression in NIH/3T3.](http://images.novusbio.com/fullsize/DDX3Y-Antibody-2D7-Western-Blot-H00008653-M01-img0011.jpg "Western Blot: DDX3 Antibody (2D7) [H00008653-M01] - DDX3Y monoclonal antibody (M01), clone 2D7. Analysis of DDX3Y expression in NIH/3T3.")
![Immunohistochemistry-Paraffin: DDX3 Antibody (2D7) [H00008653-M01] - Analysis of monoclonal antibody to DDX3Y on formalin-fixed paraffin-embedded human testis. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/DDX3Y-Antibody-2D7-Immunohistochemistry-Paraffin-H00008653-M01-img0007.jpg)
![Western Blot: DDX3 Antibody (2D7) [H00008653-M01] - DDX3Y monoclonal antibody (M01), clone 2D7. Analysis of DDX3Y expression in Raw 264.7.](http://images.novusbio.com/fullsize/DDX3Y-Antibody-2D7-Western-Blot-H00008653-M01-img0010.jpg)
![Western Blot: DDX3 Antibody (2D7) [H00008653-M01] - DDX3Y monoclonal antibody (M01), clone 2D7. Analysis of DDX3Y expression in mouse testis.](http://images.novusbio.com/fullsize/DDX3Y-Antibody-2D7-Western-Blot-H00008653-M01-img0012.jpg)
![Sandwich ELISA: DDX3 Antibody (2D7) [H00008653-M01] - Detection limit for recombinant GST tagged DDX3Y is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/DDX3Y-Antibody-2D7-Sandwich-ELISA-H00008653-M01-img0008.jpg)
![Western Blot: DDX3 Antibody [NB200-196] - Detection of DDX3 by Western Blot and Immunoprecipitation. Samples: A. Whole cell lysate from HeLa (5, 15 and 50 mcg) and Ramos (50 mcg) cells. B. Whole cell lysate (1 mg/IP; 1/4 of IP loaded/lane) from HeLa cells. Antibodies: NB 200-196 used at 0.04 mcg/ml (A) and 0.1 mcg/ml (B) for Western Blot and at 3 mcg/mg lysate for IP (B). DDX3 was also immunoprecipitated using NB 200-194 and NB 200-195 at 3 mcg/mg lysate. Detection: Chemiluminescence with an exposure time of 10 seconds (A and B).](http://images.novusbio.com/fullsize/DDX3-Antibody-Western-Blot-NB200-196-img0002.jpg "<b>Independent Antibodies Validation. </b>Western Blot: DDX3 Antibody [NB200-196] - Detection of DDX3 by Western Blot and Immunoprecipitation. Samples: A. Whole cell lysate from HeLa (5, 15 and 50 mcg) and Ramos (50 mcg) cells. B. Whole cell lysate (1 mg/IP; 1/4 of IP loaded/lane) from HeLa cells. Antibodies: NB 200-196 used at 0.04 mcg/ml (A) and 0.1 mcg/ml (B) for Western Blot and at 3 mcg/mg lysate for IP (B). DDX3 was also immunoprecipitated using NB 200-194 and NB 200-195 at 3 mcg/mg lysate. Detection: Chemiluminescence with an exposure time of 10 seconds (A and B).")