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![Immunocytochemistry/Immunofluorescence: Actin Alpha 1 Cardiac Muscle Antibody (22D3) [NBP1-97725] - Immunofluorescence staining of developing somites in stage 46 Xenopus laevis embryo using Actin Alpha 1 Cardiac Muscle Antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/Actin-Alpha-Skeletal-Cardiac-Antibody-22D3-Immunofluorescence-NBP1-97725-img0004.jpg "Immunocytochemistry/Immunofluorescence: Actin Alpha 1 Cardiac Muscle Antibody (22D3) [NBP1-97725] - Immunofluorescence staining of developing somites in stage 46 Xenopus laevis embryo using Actin Alpha 1 Cardiac Muscle Antibody. Image from verified customer review.")
![Immunohistochemistry-Paraffin: Actin Alpha 1 Cardiac Muscle Antibody (22D3) [NBP1-97725] - Paraffin section of human cardiac muscle immunostained with 22D3 (1:500)](http://images.novusbio.com/fullsize/Actin-Alpha-Skeletal-Cardiac-Antibody-22D3-Immunohistochemistry-Paraffin-NBP1-97725-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Actin Alpha 1 Cardiac Muscle Antibody (22D3) [NBP1-97725] - Immunofluorescence staining of muscle tissue in the tail of 3 days old zebrafish embryo](http://images.novusbio.com/fullsize/Actin-Alpha-Skeletal-Cardiac-Antibody-22D3-Immunocytochemistry-Immunofluorescence-NBP1-97725-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Actin Alpha 1 Cardiac Muscle Antibody (22D3) [NBP1-97725] - Immunofluorescence staining of developing myocardium in 1 month old zebrafish embryo](http://images.novusbio.com/fullsize/Actin-Alpha-Skeletal-Cardiac-Antibody-22D3-Immunocytochemistry-Immunofluorescence-NBP1-97725-img0003.jpg)
![Western Blot: Actin Alpha 1 Cardiac Muscle Antibody (RM257) [NBP2-61474] - Western Blot of Mouse heart tissue lysate, using NBP2-61474 at a 1:1000 dilution](http://images.novusbio.com/fullsize/Actin-Alpha-1-Cardiac-Muscle-Antibody-RM257-Western-Blot-NBP2-61474-img0003.jpg "Western Blot: Actin Alpha 1 Cardiac Muscle Antibody (RM257) [NBP2-61474] - Western Blot of Mouse heart tissue lysate, using NBP2-61474 at a 1:1000 dilution")
![Immunohistochemistry: Actin Alpha 1 Cardiac Muscle Antibody (RM257) [NBP2-61474] - Immunohistochemical staining of FFPE Human heart tissue sections using NBP2-61474 at a 1:1000 dilution](http://images.novusbio.com/fullsize/Actin-Alpha-1-Cardiac-Muscle-Antibody-RM257-Immunohistochemistry-NBP2-61474-img0002.jpg)
![Western Blot: Actin Alpha 1 Cardiac Muscle Antibody [NBP1-33759] - Sample (30 ug of whole cell lysate) A: NIH-3T3 10% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Actin-Alpha-1-Cardiac-Muscle-Antibody-Western-Blot-NBP1-33759-img0002.jpg "Western Blot: Actin Alpha 1 Cardiac Muscle Antibody [NBP1-33759] - Sample (30 ug of whole cell lysate) A: NIH-3T3 10% SDS PAGE; antibody diluted at 1:1000.")
![Immunohistochemistry-Paraffin: Actin Alpha 1 Cardiac Muscle Antibody [NBP1-33759] - Mouse muscle. alpha Cardiac Muscle Actin antibody diluted at 1:500.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Actin-Alpha-1-Cardiac-Muscle-Antibody-Immunohistochemistry-Paraffin-NBP1-33759-img0005.jpg)
![Immunohistochemistry-Paraffin: Actin Alpha 1 Cardiac Muscle Antibody [NBP1-33759] - Mouse heart. alpha Cardiac Muscle Actin antibody diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Actin-Alpha-1-Cardiac-Muscle-Antibody-Immunohistochemistry-Paraffin-NBP1-33759-img0003.jpg)
![Immunohistochemistry-Paraffin: Actin Alpha 1 Cardiac Muscle Antibody [NBP1-33759] - Mouse muscle. alpha Cardiac Muscle Actin antibody diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Actin-Alpha-1-Cardiac-Muscle-Antibody-Immunohistochemistry-Paraffin-NBP1-33759-img0004.jpg)
![Western Blot: Actin Alpha 1 Cardiac Muscle Antibody (33-32-R) [NBP3-32032] - Western blot analysis of Actin Alpha 1 Cardiac Muscle on different lysates with Mouse anti-Actin Alpha 1 Cardiac Muscle antibody (NBP3-32032) at 1/1,000 dilution. Lane 1: Mouse lung tissue lysate Lane 2: Mouse heart tissue lysate Lane 3: Rat heart tissue lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32032) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32032_mouse-actin-alpha-1-cardiac-muscle-mab-33-32-r-278202415141712.jpg "Western Blot: Actin Alpha 1 Cardiac Muscle Antibody (33-32-R) [NBP3-32032] - Western blot analysis of Actin Alpha 1 Cardiac Muscle on different lysates with Mouse anti-Actin Alpha 1 Cardiac Muscle antibody (NBP3-32032) at 1/1,000 dilution. Lane 1: Mouse lung tissue lysate Lane 2: Mouse heart tissue lysate Lane 3: Rat heart tissue lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32032) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Actin Alpha 1 Cardiac Muscle Antibody (33-32-R) [NBP3-32032] - Immunofluorescence analysis of paraffin-embedded human heart tissue labeling Actin Alpha 1 Cardiac Muscle with Mouse anti-Actin Alpha 1 Cardiac Muscle antibody (NBP3-32032) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32032, green) at 1/1,000 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32032_mouse-actin-alpha-1-cardiac-muscle-mab-33-32-r-29820241420918.jpg)
![Immunohistochemistry: Actin Alpha 1 Cardiac Muscle Antibody (33-32-R) [NBP3-32032] - Immunohistochemical analysis of paraffin-embedded human heart tissue with Mouse anti-Actin Alpha 1 Cardiac Muscle antibody (NBP3-32032) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32032) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32032_mouse-actin-alpha-1-cardiac-muscle-mab-33-32-r-29820241420938.jpg)
![Western Blot: Actin Alpha 1 Cardiac Muscle Overexpression Lysate (Adult Normal) [NBL1-07273] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Actin Alpha 1 Cardiac Muscle.](http://images.novusbio.com/fullsize/Actin-Alpha-1-Cardiac-Muscle-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-07273-img0002.jpg "Western Blot: Actin Alpha 1 Cardiac Muscle Overexpression Lysate (Adult Normal) [NBL1-07273] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Actin Alpha 1 Cardiac Muscle.")