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![Immunocytochemistry/Immunofluorescence: MHC Class I Antibody (OX18) [NB120-6405] - Major histocompatibility complex (MHC) I and II as well as Transporter associated with antigen presentation II (TAPII) were analyzed, using immunocytochemistry on rat Schwann cells (SCs). Corresponding merges are shown in the bottom rows. Treatment of SCs with IL-17 was performed at concentrations of 0.5 and 50 ng/mL. Graphs to the right show densitometry quantification. SCs showed expression of MHCI > TAPII > MHCII, which increased after IL-17 treatment. MHCI was mainly detected in the cytoplasm and the expression increased in a dose-dependent manner after IL-17 treatment, significant for 0.5 ng/mL and 50 ng/mL (**P <=0.01). Image collected and cropped by CiteAb from the following publication (//jneuroinflammation.biomedcentral.com/articles/10.1186/1742-2094-11-63), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/MHC-Class-I-Antibody-OX18-Immunocytochemistry-Immunofluorescence-NB120-6405-img0009.jpg "Immunocytochemistry/Immunofluorescence: MHC Class I Antibody (OX18) [NB120-6405] - Major histocompatibility complex (MHC) I and II as well as Transporter associated with antigen presentation II (TAPII) were analyzed, using immunocytochemistry on rat Schwann cells (SCs). Corresponding merges are shown in the bottom rows. Treatment of SCs with IL-17 was performed at concentrations of 0.5 and 50 ng/mL. Graphs to the right show densitometry quantification. SCs showed expression of MHCI > TAPII > MHCII, which increased after IL-17 treatment. MHCI was mainly detected in the cytoplasm and the expression increased in a dose-dependent manner after IL-17 treatment, significant for 0.5 ng/mL and 50 ng/mL (**P <=0.01). Image collected and cropped by CiteAb from the following publication (//jneuroinflammation.biomedcentral.com/articles/10.1186/1742-2094-11-63), licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: MHC Class I Antibody (OX18) [NB120-6405] - Analysis of FFPE rat brain cerebellum using MHC Class I (OK18) antibody at 1:200 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Endothelial staining was observed. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/MHC-Class-I-Antibody-OX18-Immunohistochemistry-Paraffin-NB120-6405-img0008.jpg)
![Flow Cytometry: MHC Class I Antibody (OX18) [NB120-6405] - Analysis using the FITC conjugate of NB120-6405. Staining of rat spleen cells with mouse anti-rat RT1-A (OX18).](http://images.novusbio.com/fullsize/MHC-Class-I-Antibody-OX18-Flow-Cytometry-NB120-6405-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: MHC Class I Antibody (OX18) [NB120-6405] - PC-12 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-MHC Class I (OX18) NB120-6405 at a 1:100 dilution overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/MHC-Class-I-Antibody-OX18-Immunocytochemistry-Immunofluorescence-NB120-6405-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: MHC Class I Antibody (OX18) [NB120-6405] - PC12 cells were fixed for 10 minutes using 4% PFA and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-MHC Class I Antibody (OX18) at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/MHC-Class-I-Antibody-OX18-Immunocytochemistry-Immunofluorescence-NB120-6405-img0010.jpg)
![Flow (Intracellular): MHC Class I Antibody (OX18) [NB120-6405] - PC-12 cells were stained with MHC Class I NB120-6405 (Blue) and a matched isotype control NBP2-27287 (Orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by DyLight488-conjugated anti-mouse secondary antibody.](http://images.novusbio.com/fullsize/MHC-Class-I-Antibody-OX18-Flow-Intracellular-NB120-6405-img0004.jpg)
![Immunohistochemistry: MHC Class I Antibody (OX18) - BSA Free [NB120-6405] - Effects of anodal tDCS on protein expression. Representative immunohistochemical images of Ox18 + cells in the SVZ ipsilateral to anodal tDCS or sham stimulation. Staining for Ox18 (MHC I) in the ipsilateral SVZ revealed more Ox18 + cells in animals treated by anodal tDCS (left panel) compared to sham stimulation (right panel) by trend (scale bars = 100 μm). Results are displayed as mean ± SEM. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31708742), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb120-6405_mouse-monoclonal-mhc-class-i-antibody-ox18-31020241534348.jpg)
![Western Blot: MHC Class I Antibody (JF10-38) [NBP2-66946] - Analysis of MHC class I on different lysates with Rabbit anti-MHC class I antibody at 1/2,000 dilution.Lane 1: HL-60 cell lysateLane 2: K-562 cell lysate (low expression)Lysates/proteins at 15 µg/Lane.Predicted band size: 41 kDaObserved band size: 41 kDaExposure time: 5 minutes;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4c overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-66946_rabbit-monoclonal-mhc-class-i-antibody-jf10-38-western-blot-362024112212..jpg "Western Blot: MHC Class I Antibody (JF10-38) [NBP2-66946] - Analysis of MHC class I on different lysates with Rabbit anti-MHC class I antibody at 1/2,000 dilution.Lane 1: HL-60 cell lysateLane 2: K-562 cell lysate (low expression)Lysates/proteins at 15 µg/Lane.Predicted band size: 41 kDaObserved band size: 41 kDaExposure time: 5 minutes;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4c overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry-Paraffin: MHC Class I Antibody (JF10-38) [NBP2-66946] - Analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-MHC class I antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp2-66946_rabbit-monoclonal-mhc-class-i-antibody-jf10-38-immunohistochemistry-paraffin-362024112318..jpg)
![Immunohistochemistry-Paraffin: MHC Class I Antibody (JF10-38) [NBP2-66946] - Analysis of paraffin-embedded human liver cancer tissue with Rabbit anti-MHC class I antibody at 1/200 dilution.](http://images.novusbio.com/fullsize/nbp2-66946_rabbit-monoclonal-mhc-class-i-antibody-jf10-38-immunohistochemistry-paraffin-362024112412..jpg)
![Immunohistochemistry-Paraffin: MHC Class I Antibody (JF10-38) [NBP2-66946] - Analysis of paraffin-embedded human tonsil tissue with Rabbit anti-MHC class I antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp2-66946_rabbit-monoclonal-mhc-class-i-antibody-jf10-38-immunohistochemistry-paraffin-362024112523..jpg)
![Immunohistochemistry-Paraffin: MHC Class I Antibody (JF10-38) [NBP2-66946] - Analysis of paraffin-embedded human pancreas tissue with Rabbit anti-MHC class I antibody at 1/200 dilution.](http://images.novusbio.com/fullsize/nbp2-66946_rabbit-monoclonal-mhc-class-i-antibody-jf10-38-immunohistochemistry-paraffin-362024112620..jpg)
![Flow Cytometry: MHC Class I Antibody (JF10-38) [NBP2-66946] - Analysis of HL-60 cells labeling MHC class I.Cells were fixed and permeabilized. Then stained with the primary antibody (1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4c for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4c. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp2-66946_rabbit-monoclonal-mhc-class-i-antibody-jf10-38-flow-cytometry-362024112742..jpg)
![Immunocytochemistry/Immunofluorescence: MHC Class I Antibody (JF10-38) [NBP2-66946] - Analysis of paraffin-embedded human tonsil tissue labeling MHC class I with Rabbit anti-MHC class I antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (green) at 1/200 dilution overnight at 4 c, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp2-66946_rabbit-monoclonal-mhc-class-i-antibody-jf10-38-immunocytochemistry-immunofluorescence-362024112932..jpg)
![Western Blot: MHC Class I Antibody (R1-21.2) - Chimeric [NBP3-09017] - Mouse thymus tissue lysate (35ug protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with NBP3-09017 at 1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/MHC-Class-I-Antibody-R1-21-2-Chimeric-Western-Blot-NBP3-09017-img0003.jpg "Western Blot: MHC Class I Antibody (R1-21.2) - Chimeric [NBP3-09017] - Mouse thymus tissue lysate (35ug protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with NBP3-09017 at 1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: MHC Class I Antibody (R1-21.2) - Chimeric [NBP3-09017] - Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized and stained with NBP3-09017 at 10 ug/ml followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing membrane staining in a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-09017, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (NBP2-52638) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/MHC-Class-I-Antibody-R1-21-2-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-09017-img0002.jpg)
![Immunohistochemistry: MHC Class I Antibody (MEM-E/06) [NB500-307] - Staining of human spleen (paraffin sections) with anti-HLA-E (clone MEM-E/06).](http://images.novusbio.com/fullsize/MHC-Class-I-Antibody-MEM-E-06-Immunohistochemistry-NB500-307-img0001.jpg "Immunohistochemistry: MHC Class I Antibody (MEM-E/06) [NB500-307] - Staining of human spleen (paraffin sections) with anti-HLA-E (clone MEM-E/06).")
![Flow Cytometry: MHC Class I Antibody (MEM-E/06) [NB500-307] - Analysis using PE conjugate of NB500-307. A cell surface stain was performed on THP-1 cells with MHC Class I antibody (MEM-E/06) NB500-307 (blue) and a matched isotype control NBP2-27287 (orange). Cells were incubated in an antibody dilution of 1:100 for 20 minutes at room temperature. Both antibodies were conjugated to PE.](http://images.novusbio.com/fullsize/MHC-Class-I-Antibody-MEM-E-06-Flow-Cytometry-NB500-307-img0002.jpg)
![Flow Cytometry: MHC Class I Antibody (H58A) [NBP2-61002] - Obtained with canine leukocytes.](http://images.novusbio.com/fullsize/MHC-Class-I-Antibody-H58A-Flow-Cytometry-NBP2-61002-img0002.jpg "Flow Cytometry: MHC Class I Antibody (H58A) [NBP2-61002] - Obtained with canine leukocytes.")
![Flow Cytometry: MHC Class I Antibody (H58A) [NBP2-61002] - Obtained with feline leukocytes.](http://images.novusbio.com/fullsize/MHC-Class-I-Antibody-H58A-Flow-Cytometry-NBP2-61002-img0001.jpg)
![Western Blot: HLA-E Antibody [NBP2-14985] - Sample (30 ug of whole cell lysate) A: Raji B: K562 C: THP-1 D: HL-60 10% SDS PAGE; antibody diluted at 1:10000.](http://images.novusbio.com/fullsize/HLA-E-Antibody-Western-Blot-NBP2-14985-img0002.jpg "Western Blot: HLA-E Antibody [NBP2-14985] - Sample (30 ug of whole cell lysate) A: Raji B: K562 C: THP-1 D: HL-60 10% SDS PAGE; antibody diluted at 1:10000.")
![Immunohistochemistry-Paraffin: HLA-E Antibody [NBP2-14985] - U87 xenograft, using HLA-E antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/HLA-E-Antibody-Immunohistochemistry-Paraffin-NBP2-14985-img0001.jpg)
![Western Blot: MHC Class I Antibody (3I1C1) [NBP3-16696] - Western blot analysis of extracts of various cell lines, using MHC Class I Rabbit mAb (NBP3-16696) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/MHC-Class-I-Antibody-3I1C1-Western-Blot-NBP3-16696-img0002.jpg "Western Blot: MHC Class I Antibody (3I1C1) [NBP3-16696] - Western blot analysis of extracts of various cell lines, using MHC Class I Rabbit mAb (NBP3-16696) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunocytochemistry/Immunofluorescence: MHC Class I Antibody (3I1C1) [NBP3-16696] - Immunofluorescence analysis of THP-1 cells using MHC Class I antibody (NBP3-16696) at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/MHC-Class-I-Antibody-3I1C1-Immunocytochemistry-Immunofluorescence-NBP3-16696-img0001.jpg)