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![Immunocytochemistry/Immunofluorescence: HYPB Antibody [NBP3-03807] - Analysis of U-2 OS cells using HYPB antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/HYPB-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-03807-img0002.jpg "Immunocytochemistry/Immunofluorescence: HYPB Antibody [NBP3-03807] - Analysis of U-2 OS cells using HYPB antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.")
![Immunocytochemistry/Immunofluorescence: HYPB Antibody [NBP3-03807] - Analysis of C6 cells using HYPB antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/HYPB-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-03807-img0001.jpg)
![Western Blot: HYPB Antibody [NBP3-03807] - analysis of lysates from Mouse spleen, using SETD2 Rabbit pAb at 1:900 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/antibody/nbp3-03807_rabbit-polyclonal-hypb-antibody-western-blot-19122023111418..jpg)
![Immunoprecipitation- HYPB Antibody [NBP3-03807] - Analysis of 600 μg extracts of Mouse spleen using 3 μg SETD2 antibody. Western blot was performed from the immunoprecipitate using SETD2 antibody at a dilution of 1:1000.](http://images.novusbio.com/fullsize/antibody/nbp3-03807_rabbit-polyclonal-hypb-antibody-immunoprecipitation-19122023111617..jpg)
![Immunohistochemistry-Paraffin: HYPB Antibody (CL9956) [NBP2-88941] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/HYPB-Antibody-CL9956-Immunohistochemistry-Paraffin-NBP2-88941-img0004.jpg "Immunohistochemistry-Paraffin: HYPB Antibody (CL9956) [NBP2-88941] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.")
![Immunohistochemistry-Paraffin: HYPB Antibody (CL9956) [NBP2-88941] - Staining of human cervix, uterine shows moderate to strong nuclear positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/HYPB-Antibody-CL9956-Immunohistochemistry-Paraffin-NBP2-88941-img0001.jpg)
![Immunohistochemistry-Paraffin: HYPB Antibody (CL9956) [NBP2-88941] - Staining of human endometrium shows moderate nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/HYPB-Antibody-CL9956-Immunohistochemistry-Paraffin-NBP2-88941-img0002.jpg)
![Immunohistochemistry-Paraffin: HYPB Antibody (CL9956) [NBP2-88941] - Staining of human skeletal muscle shows very weak positivity in myocytes as expected.](http://images.novusbio.com/fullsize/HYPB-Antibody-CL9956-Immunohistochemistry-Paraffin-NBP2-88941-img0003.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: HYPB Antibody (CL9956) [NBP2-88941] - Analysis in human testis and skeletal muscle tissues using NBP2-88941 antibody. Corresponding SETD2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/HYPB-Antibody-CL9956-Immunohistochemistry-Paraffin-NBP2-88941-img0005.jpg)
![Western Blot: HYPB Antibody (1E1) [NBP2-45791] - Human recombinant protein fragment corresponding to amino acids 1787-2144 of human SETD2 (NP_085487) produced in E.coli.](http://images.novusbio.com/fullsize/HYPB-Antibody-1E1-Western-Blot-NBP2-45791-img0002.jpg "Western Blot: HYPB Antibody (1E1) [NBP2-45791] - Human recombinant protein fragment corresponding to amino acids 1787-2144 of human SETD2 (NP_085487) produced in E.coli.")
![Immunohistochemistry: HYPB Antibody (1E1) [NBP2-45791] - Analysis of Human embryonic liver tissue.(Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/HYPB-Antibody-1E1-Immunohistochemistry-NBP2-45791-img0001.jpg)
![Flow Cytometry: HYPB Antibody [NB100-77334] - Analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation overnight (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/HYPB-Antibody-Flow-Cytometry-NB100-77334-img0003.jpg "Flow Cytometry: HYPB Antibody [NB100-77334] - Analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation overnight (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.")
![Western Blot: HYPB Antibody [NBP2-98651] - Anti-HYPB rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 288 kDa. Observed band size: 315 kDa (We are unsure as to the identity of these extra bands).](http://images.novusbio.com/fullsize/HYPB-Antibody-Western-Blot-NBP2-98651-img0002.jpg "Western Blot: HYPB Antibody [NBP2-98651] - Anti-HYPB rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 288 kDa. Observed band size: 315 kDa (We are unsure as to the identity of these extra bands).")
![Western Blot: HYPB Antibody [NBP3-03806] - Analysis of extracts of various cell lines, using HYPB antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.](http://images.novusbio.com/fullsize/HYPB-Antibody-Western-Blot-NBP3-03806-img0001.jpg "Western Blot: HYPB Antibody [NBP3-03806] - Analysis of extracts of various cell lines, using HYPB antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.")
![Immunocytochemistry/Immunofluorescence: HYPB Antibody [NBP1-89843] - Immunofluorescent staining of human cell line A-431 shows localization to nuclear speckles & cytosol.](http://images.novusbio.com/fullsize/HYPB-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89843-img0006.jpg "Immunocytochemistry/Immunofluorescence: HYPB Antibody [NBP1-89843] - Immunofluorescent staining of human cell line A-431 shows localization to nuclear speckles & cytosol.")
![Immunohistochemistry-Paraffin: HYPB Antibody [NBP1-89843] - Staining of human small intestine shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/HYPB-Antibody-Immunohistochemistry-Paraffin-NBP1-89843-img0009.jpg)
![Immunohistochemistry-Paraffin: HYPB Antibody [NBP1-89843] - Staining of human endometrium shows strong nuclear positivity in glandular and stromal cells.](http://images.novusbio.com/fullsize/HYPB-Antibody-Immunohistochemistry-Paraffin-NBP1-89843-img0007.jpg)
![Immunohistochemistry-Paraffin: HYPB Antibody [NBP1-89843] - Staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/HYPB-Antibody-Immunohistochemistry-Paraffin-NBP1-89843-img0008.jpg)
![Immunohistochemistry-Paraffin: HYPB Antibody [NBP1-89843] - Staining of human cerebellum shows moderate nuclear positivity in Purkinje cells as well as in cells in molecular layer.](http://images.novusbio.com/fullsize/HYPB-Antibody-Immunohistochemistry-Paraffin-NBP1-89843-img0010.jpg)