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Western Blot

Detecting HIF alpha and beyond: Best controls for hypoxia Western blot analysis

Analysis of HIF-1 alpha expression in whole cell lysates from HeLa cells in culture treated or untreated with cobalt chloride and independent antibody validation where two different antibodies to HIF-1 alpha were used for immunoprecipitation and detection.

By Rosa Moreno, PhD.

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Tips to Optimize Your Western Blot for Phosphorylated Protein Detection

Western blotting allows identifying and quantifying proteins from cell lysates for determination of protein expression, sub-cellular localization, post-translational modifications, protein processing, and protein-protein interactions.

By Jamshed Arslan, Pharm. D., PhD.

Tags:

Secondary antibodies

Phosphorylated proteins

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Considerations for Quantitative Western blotting

GAPDH expression in human nucleus pulposus cell lysate, WB

By Jamshed Arslan, Pharm. D., PhD.

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Best genes to knockout to control autophagic flux

By Christina Towers, PhD

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Autophagic flux

Autophagy Inhibitors

Christina Towers

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The LC3 A, B, C’s and 1, 2, 3’s

By Christina Towers, PhD

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Autophagy Inhibitors

Christina Towers

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Best way to quantitatively measure Autophagic Flux

By Christina Towers, PhD

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Autophagy Inhibitors

Christina Towers

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Wet or dry protein transfer: Which one to choose for your western blotting?

Novus Biologicals western blot resources page

By Jamshed Arslan, Pharm. D., PhD.

Tags:

Protein transfer

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Sample collection from mammalian culture cells for kinomic analysis

By Jamshed Arslan Pharm.D., PhD.

Tags:

Phosphotyrosine

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Best Methods to Induce and Inhibit Autophagy Pharmacologically

By Christina Towers, PhD

Tags:

autophagy induction

autophagy inhibition

Christina Towers

Autophagic flux

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Losing memory: Toxicity from mutant APP and amyloid beta explain the hippocampal neuronal damage in Alzheimer's disease

By Jamshed Arslan Pharm.D.

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