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Western blot

Detecting HIF alpha and beyond: Best controls for hypoxia Western blot analysis

Analysis of HIF-1 alpha expression in whole cell lysates from HeLa cells in culture treated or untreated with cobalt chloride and independent antibody validation where two different antibodies to HIF-1 alpha were used for immunoprecipitation and detection.

By Rosa Moreno, PhD.

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Glypican 3 as a biomarker for gastro-esophageal adenocarcinoma

Expression of CD81 was detected by immunofluorescence with a mouse monoclonal antibody followed by secondary antibody labeled with DyLight488 and Phalloidin 568 staining for actin visualization

By Jamshed Arslan, Pharm. D., PhD.

Tags:

Exosome Markers

Carcinoembryonic antigen

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Tips to Optimize Your Western Blot for Phosphorylated Protein Detection

Western blotting allows identifying and quantifying proteins from cell lysates for determination of protein expression, sub-cellular localization, post-translational modifications, protein processing, and protein-protein interactions.

By Jamshed Arslan, Pharm. D., PhD.

Tags:

Secondary antibodies

Phosphorylated proteins

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Chromatin reader domains of DNMT-targeting protein, UHRF1, are responsible for cancerous DNA hypermethylation

Expression of DNMT2 in human colon tissue shows cytoplasmic and nuclear staining in glandular cells, IHC

By Jamshed Arslan, Pharm. D., PhD.

Tags:

Cancer Research

Immunohistochemistry

prostasin-prss8

Considerations for Quantitative Western blotting

GAPDH expression in human nucleus pulposus cell lysate, WB

By Jamshed Arslan, Pharm. D., PhD.

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Wet or dry protein transfer: Which one to choose for your western blotting?

Novus Biologicals western blot resources page

By Jamshed Arslan, Pharm. D., PhD.

Tags:

Protein transfer

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Best Methods to Induce and Inhibit Autophagy Pharmacologically

By Christina Towers, PhD

Tags:

autophagy induction

autophagy inhibition

Christina Towers

Autophagic flux

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Losing memory: Toxicity from mutant APP and amyloid beta explain the hippocampal neuronal damage in Alzheimer's disease

By Jamshed Arslan Pharm.D.

Application Focus: I see an increase in LC3, now what?

By Christina Towers, PhD.

Tags:

Transcription Regulation

Christina Towers

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Crosstalk Between Oxidative Stress and Autophagy

By Christina Towers, PhD.

Tags:

Glutathione Peroxidase

Oxidative Stress

Christina Towers

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