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LC3B

Autophagy and RAS signaling: Clinical implications

Immunocytochemical staining of fixed and permeabilized HeLa cells treated with chloroquine, incubated with LC3B (green) and tubulin (red) antibodies, and counterstained with DAPI to visualized DNA.

By Christina Towers, PhD

Tags: 
Autophagy
Cancer
Pancreatic Ductal Adenocarcinoma
Melanoma
Colorectal Cancer
Ras
LC3B
Beclin 1
mCherry
GFP
NRAS
B-Raf
CA19-9/Sialyl Lewis A
Christina Towers

Methamphetamine with HIV induces mitochondrial dysfunction and neuronal injury through oxidative stress

Immunocytochemical staining of formalin-fixed, permeabilized PC12 cells with rabbit polyclonal DRP1 antibody and detected with Dylight 488 (green) and the nuclei counterstained with DAPI (blue).

By Jamshed Arslan, Pharm. D., PhD.

Tags: 
HIV
Human Immunodeficiency Virus
Mitochondria
Mitophagy
ROS
Oxidative Stress
GP120
Tat
Methamphetamine
neurodegeneration
Neurons
LC3B
p62
Autophagy
MAP2
DRP1
Antioxidants
Jamshed Arslan

Autophagy and Metastasis

EpCAM/TROP1 is localized by immunohistochemical analysis of human breast carcinoma tissue to the cell membrane and intercellular spaces.
 

By Christina Towers, PhD

Tags: 
Autophagy
Cancer
EMT
LC3
LC3-II
LC3B
p62/SQSTM1
Paxillin
Twist-1
epcam-trop1
Christina Towers

Optogenetic Control of Mitophagy: AMBRA1 based mitophagy switch

sAmbra1 expression and localization to mitochondria in human U-2 OS cell line, ICC

By Christina Towers, PhD

Tags: 
Autophagy
Mitophagy
LC3
LC3-II
LC3B
p62/SQSTM1
Parkin
AMBRA1
Christina Towers

Autophagic flux: Is p62 a good indicator?

p62 detection via IHC-Paraffin, Novus Biologicals

By Christina Towers, PhD

Tags: 
Autophagy
autophagosome
LC3
LC3-II
LC3B
p62/SQSTM1
alpha Tubulin
mCherry
Autophagic flux
Chloroquine
Bafilomycin-A1
ATG5
ATG7
PKC zeta
NFkB
Keap1
NRF2
ERK1
Christina Towers

Best genes to knockout to control autophagic flux

Autophagy interactive signaling pathway, Novus Biologicals, macroautophagy induction, autophagosome maturation, microautophagy, autolysosome, elongation

By Christina Towers, PhD

Tags: 
Autophagy
Autophagic flux
LC3
LC3B
LC3-II
Chloroquine
Bafilomycin
Autophagy Inhibitors
ATGs
ATG5
ATG7
Atg3
ATG12
stx17
p62-sqstm1
ATG13
Apoptosis
Christina Towers
autophagosome
Autophagy flux

Make each cell count: How to assess autophagy using flow cytometry

LC3B expression in HeLa cells treated with chloroquine overnight, ICC. Novus Biologicals

Kristy R. Howell, PhD

Tags: 
Autophagy
autophagosome
LC3
LC3B
alpha Tubulin
GFP
mCherry
Autophagy flux
Chloroquine
rapamycin
Flow cytometry
Kristy Howell
Jurkat cell
MIFC
puncta
fluorescence
Immunocytochemistry
Immunofluorescence

How to visualize autophagy by microscopy

LC3B and alpha tubulin expression in HeLa cells treated with Chloroquine, ICC, immunocytochemistry, immunofluorescence

By Christina Towers, PhD

Tags: 
Autophagy
autophagosome
LC3
LC3B
p62/SQSTM1
alpha Tubulin
GFP
Mitophagy
mCherry
Autophagic flux
Chloroquine
Christina Towers
Autophagy flux
microscopy

The LC3 A, B, C’s and 1, 2, 3’s

Atg5 expression in HeLa cells, ICC, immunocytochemistry, immunofluorescence

By Christina Towers, PhD

Tags: 
Autophagy flux
LC3
LC3B
LC3-II
Autophagy
Autophagy Inhibitors
ATG4
ATG5
ATG12
LC3A
LC3C
GABARAP
GABARAPL1
GABARAPL2
Chloroquine
Bafilomycin-A1
ATG7
ATG8
Christina Towers
autophagosome

Best way to quantitatively measure Autophagic Flux

LC3B expression in HeLa cells, ICC, immunocytochemistry, immunofluorescence

By Christina Towers, PhD

Tags: 
Autophagy flux
LC3
LC3B
LC3-II
Chloroquine
Bafilomycin
Autophagy
Autophagy Inhibitors
Christina Towers
autophagosome
GFP
Flow cytometry
ratiometric
RFP
mCherry

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