Novus Biologicals products are now on bio-techne.com

Park7/DJ-1 Antibody

Images

 
Image 1 of 11
(
Enlarge)
Simple Western: Park7/DJ-1 Antibody [NB300-270] - Simple Western lane view shows a specific band for Park7 (DJ-1) in 0.05 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Ch, Bv, SyHa, ZeSpecies Glossary
Applications WB, Simple Western, Flow, Func, ICC/IF, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
   

Biological Strategies

     

Order Details

Shipping $45.00 per order
Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Datasheet
Reviews & Publications
Protocols & FAQs
Support & Research

Park7/DJ-1 Antibody Summary

Immunogen
A synthetic peptide corresponding to the C-terminus (within residues 150-189) of human Park7(DJ-1). [UniProt# Q99497]
Localization
Nucleus. Cytoplasm. Associated with mitochondria in some cells, particularly after oxidative stress. Detected in tau inclusions in brains from neurodegenerative disease patients.
Predicted Species
Golden Syrian Hamster (100%), Zebrafish (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PARK7
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry reported in scientific literature (PMID 21937704)
  • Immunocytochemistry/ Immunofluorescence 1:500
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 1:200
  • In vitro assay reported in scientific literature (PMID 21645620)
  • Simple Western 1:4000
  • Western Blot 1:2000
Application Notes
In Western Blot, a band can be seen at approx. 25 kDa.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Theoretical MW
25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Park7/DJ-1 Overexpression Lysate
Reviewed Applications
Read 3 Reviews rated 3.7
using
NB300-270 in the following applications:

Publications
Read Publications using
NB300-270 in the following applications:

Reactivity Notes

Predicted to react with bovine, Zebra fish, and Golden Syrian hamster based on 100% sequence homology. Chicken reactivity reported in scientific literature (PMID: 24064392). Rat reactivity reported in scientific literature (PMID: 26419955)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Park7/DJ-1 Antibody

  • DJ1
  • DJ-1
  • DJ1FLJ34360
  • EC 3.4
  • FLJ27376
  • FLJ92274
  • Oncogene DJ1
  • Park7
  • Parkinson disease (autosomal recessive, early onset) 7
  • Parkinson disease protein 7
  • protein DJ-1

Background

Park7/DJ-1 is one of several genes thought to cause autosomal-recessive early-onset parkinson's disease. It also acts as a positive regulator of androgen receptor-dependent transcription, as well as functions as a redox-sensitive chaperone, as a sensor for oxidative stress, and it apparently protects neurons against oxidative stress and cell death.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-15365
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, S-ELISA, WB
BC100-494
Species: Hu, Mu, Rb, Rt
Applications: EM, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KD, KO, PEP-ELISA, PAGE, WB
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information


Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

3.7
5
2
4
0
3
0
2
0
1
1

Agnès Tessier
06/17/2019
Application: Simple Western
Species: Human

 
Verified Customer
09/10/2013
Application: WB
Species: Human

Verified Customer
12/19/2011
Application: WB
Species: Human

Bioinformatics

Gene Symbol PARK7
Entrez
Uniprot