EEA1 Antibody

Early endosomal antigen 1 (EEA1) is a 162 kDa membrane bound protein component specific to the early endosome and is essential for fusion between early endocytic vesicles. Early endosomes are cytoplasmic compartments which fuse with endocytic vesicles for redistribution of extracellular compounds to alternate destinations. Zinc finger like domains, reminiscent of those found in nucleic acid binding proteins, are located in the amino and carboxyl terminal domains of EEA1. The carboxyl terminal zinc finger like domain is conserved in several other non nuclear proteins, some of which are also involved in intracellular protein trafficking. In addition, this domain is an authentic zinc binding domain and is critical to the intracellular localization of EEA1. Membrane association of EEA1 has been shown dependent on phosphatidyl 3 kinase activity, inhibitors of which cause EEA1 to dissociate from early endosomes. Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: ICC/IF, IM, Western Blot.

Showing Publications 1 - 8 of 8.

Publications using NBP1-30914	Applications	Species
Das S, Parray H, Chiranjivi A et al. Kennedy epitope (KE)-dependent retrograde transport of efficiently cleaved HIV-1 envelopes (Envs) and its effect on Env cell surface expression and viral particle formation Research Square 2022-11-10 (Western Blot, Human)	Western Blot	Human
Momei Zhou, Benjamin Vollmer, Emily Machala, Muyuan Chen, Kay Grünewald, Ann M Arvin, Wah Chiu, Stefan L Oliver Targeted mutagenesis of the herpesvirus fusogen central helix captures transition states. Nature communications 2023-12-04 [PMID: 38042814]
Supratik Das, Hilal Ahmad Parray, Adarsh Kumar Chiranjivi, Prince Kumar, Abhishek Goswami, Manish Bansal, Deepak Kumar Rathore, Rajesh Kumar, Sweety Samal Kennedy Epitope (KE)-dependent Retrograde Transport of Efficiently Cleaved HIV-1 Envelopes (Envs) and its Effect on Env Cell Surface Expression and Viral Particle Formation. The protein journal 2023-10-04 [PMID: 37794304]
Murakami R, Chiba Y, Nishi N et al. Immunoreactivity of receptor and transporters for lactate located in astrocytes and epithelial cells of choroid plexus of human brain Neurosci Lett 2020-11-16 [PMID: 33212210] (ICC/IF, Human) Details: Immunofluorescence analysis and immunohistochemical analysis of human brain samples. Western blot analysis performed in HeLa and HT-29.	ICC/IF	Human
Yang E, Arvin AM, Oliver SL. The Glycoprotein B Cytoplasmic Domain Lysine Cluster Is Critical for Varicella Zoster Virus Cell-Cell Fusion Regulation and Infection. J. Virol. 2016-10-26 [PMID: 27795427] (IM)	IM
Yang E, Arvin AM, Oliver SL. The cytoplasmic domain of varicella-zoster virus glycoprotein h regulates syncytia formation and skin pathogenesis. PLoS Pathog. 2014-05-01 [PMID: 24874654] (ICC/IF, Human) Details: Human melanoma cells transiently expressing gH[WT] or gH mutants with gL at 24 hours post transfection were immunostained for early endosome antigen (EEA1) with anti-EEA1 antibody - immunostaining appearing as punctae in the cytoplasm (Figure 4). Human melanoma cells at 24 (Uninfected) or infection with pOka-gH[?834-841], pOka-gH[TL], pOka-gH[Y835A], pOka-gH[Y835F], pOka-gH[V5], pOka-gH[834StopV5], or pOka viruses were also stained for EEA1 (Figure 8).	ICC/IF	Human
Oliver SL, Brady JJ, Sommer MH et al. An immunoreceptor tyrosine-based inhibition motif in varicella-zoster virus glycoprotein B regulates cell fusion and skin pathogenesis. Proc Natl Acad Sci U S A 2013-01-29 [PMID: 23322733] (ICC/IF, Human)	ICC/IF	Human
Chen YT, Tsai MS, Yang TL et al. R26R-GR: a Cre-activable dual fluorescent protein reporter mouse. PLoS One 2012-01-01 [PMID: 23049968] (ICC/IF, Human)	ICC/IF	Human

Images	Ratings	Applications	Species	Date	Details
Enlarge	5 reviewed by: Luiz Alexandre Magno	WB	Human	05/05/2019	View Summary Application Western Blot Sample Tested HEK 293 whole cell lysate Species Human Lot 39959 Comments Comments Antibody concentration: 1:1,000 Incubation time/temp: overnight at 4C (shaking)

Array NBP1-30914

• EEA1 Antibodies
• EEA1 ELISA Kits
• EEA1 Lysate
• EEA1 Proteins