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![Immunohistochemistry: GLUT9 Antibody [NBP1-05054] - Immunofluorescence staining of GLUT9 in PFA-fixed murine brain sections. Frozen sections of paraformaldehyde-fixed wild-type murine brain were used for immunofluorescence staining. a, b Antigen absorption test. Immunofluorescence staining of the ependymal wall of the dorsal third ventricle using a anti-GLUT9 antibody and b antigen-preabsorbed antibody. Scale bar 100 um. c, d Immunofluorescence staining of GLUT9 (magenta), acetylated-tubulin (Ac-Tubulin, green) and DAPI (blue) on ependymal cells. Scale bar 10 um. e-g Immunofluorescence staining of GLUT9 (magenta) and NeuN (green) showing co-localization in neurons. Scale bar 10 um. Image collected and cropped by CiteAb from the following publication (//fluidsbarrierscns.biomedcentral.com/articles/10.1186/s12987-016-0046-x) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/GLUT9-Antibody-Immunohistochemistry-NBP1-05054-img0006.jpg "Immunohistochemistry: GLUT9 Antibody [NBP1-05054] - Immunofluorescence staining of GLUT9 in PFA-fixed murine brain sections. Frozen sections of paraformaldehyde-fixed wild-type murine brain were used for immunofluorescence staining. a, b Antigen absorption test. Immunofluorescence staining of the ependymal wall of the dorsal third ventricle using a anti-GLUT9 antibody and b antigen-preabsorbed antibody. Scale bar 100 um. c, d Immunofluorescence staining of GLUT9 (magenta), acetylated-tubulin (Ac-Tubulin, green) and DAPI (blue) on ependymal cells. Scale bar 10 um. e-g Immunofluorescence staining of GLUT9 (magenta) and NeuN (green) showing co-localization in neurons. Scale bar 10 um. Image collected and cropped by CiteAb from the following publication (//fluidsbarrierscns.biomedcentral.com/articles/10.1186/s12987-016-0046-x) licensed under a CC-BY license.")
![Immunohistochemistry: GLUT9 Antibody [NBP1-05054] - GLUT9 in methacarn-fixed murine brain. Paraffin sections of methacarn-fixed murine brain were used for immunostaining. a, b Antigen absorption test. Immunohistochemistry using showing a anti-GLUT9 antibody staining in the third ventricle ependyma and parenchyma and b antigen-preabsorbed antibody. Scale bar 100 um. c GLUT9 immunoreactivity observed in brain capillaries in the cortex. Scale bar 100 um. d-f Immunofluorescence staining of d GLUT9 and e P-gp showing co-localization in capillaries. Blue indicates DAPI-stained nucleus of the brain capillary endothelial cell. Scale bar 10 um.Image collected and cropped by CiteAb from the following publication (//fluidsbarrierscns.biomedcentral.com/articles/10.1186/s12987-016-0046-x) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/GLUT9-Antibody-Immunohistochemistry-NBP1-05054-img0007.jpg)
![Western Blot: GLUT9 Antibody [NBP1-05054] - Detection of GLUT9 in human kidney membrane.](http://images.novusbio.com/fullsize/GLUT9-Antibody-Western-Blot-NBP1-05054-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: GLUT9 Antibody [NBP1-05054] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-GLUT9 at a 1:200 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/GLUT9-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-05054-img0005.jpg)
![Immunohistochemistry-Paraffin: GLUT9 Antibody [NBP1-05054] - IHC-P analysis of mouse kidney section using GLUT9 antibody at 1:200 dilution. This antibody generated an expected cytoplasmic staining in tubular cells with a more staining towards membranes in some cells but no immunopositivity in Bowmans capsules.](http://images.novusbio.com/fullsize/GLUT9-Antibody-Immunohistochemistry-Paraffin-NBP1-05054-img0004.jpg)
![Western Blot: GLUT9 Antibody [NBP1-06271] - Detection of GLUT9 in mouse kidney membrane using NBP1-06271.](http://images.novusbio.com/fullsize/GLUT9-Antibody-Western-Blot-NBP1-06271-img0003.jpg "Western Blot: GLUT9 Antibody [NBP1-06271] - Detection of GLUT9 in mouse kidney membrane using NBP1-06271.")
![Immunohistochemistry-Paraffin: GLUT9 Antibody [NBP1-06271] - IHC-P analysis of a mouse kidney section with GLUT9 antibody at 1:200 dilution. The antibody generated an expected staining with more intensity towards the basolateral membranes of renal tubular epithelial cells.](http://images.novusbio.com/fullsize/GLUT9-Antibody-Immunohistochemistry-Paraffin-NBP1-06271-img0004.jpg)
![Western Blot: GLUT9 Antibody [NBP1-06565] - Detection of GLUT9a in human kidney membrane prep.](http://images.novusbio.com/fullsize/GLUT9-Antibody-Western-Blot-NBP1-06565-img0002.jpg "Western Blot: GLUT9 Antibody [NBP1-06565] - Detection of GLUT9a in human kidney membrane prep.")
![Immunohistochemistry-Paraffin: GLUT9 Antibody [NBP1-06565] - IHC-P analysis mouse kidney section with GLUT9 antibody at 1:200 dilution. This antibody gave the expected staining of SLC2A9-L isoform in the tubular epithelial cells and the blood vessel endothelium.](http://images.novusbio.com/fullsize/GLUT9-Antibody-Immunohistochemistry-Paraffin-NBP1-06565-img0003.jpg)
![Immunohistochemistry-Paraffin: GLUT9 Antibody [NBP2-47448] - Staining of human lymph node shows low expression as expected.](http://images.novusbio.com/fullsize/GLUT9-Antibody-Immunohistochemistry-Paraffin-NBP2-47448-img0002.jpg "Immunohistochemistry-Paraffin: GLUT9 Antibody [NBP2-47448] - Staining of human lymph node shows low expression as expected.")
![Immunohistochemistry: GLUT9 Antibody [NBP2-47448] - Staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/GLUT9-Antibody-Immunohistochemistry-NBP2-47448-img0001.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: GLUT9 Antibody [NBP2-47448] - Staining in human kidney and lymph node tissues using anti-SLC2A9 antibody. Corresponding SLC2A9 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/GLUT9-Antibody-Immunohistochemistry-Paraffin-NBP2-47448-img0003.jpg)
![Immunohistochemistry-Paraffin: GLUT9 Antibody [NBP3-16970] - Staining of human cerebral cortex shows low expression as expected.](http://images.novusbio.com/fullsize/GLUT9-Antibody-Immunohistochemistry-Paraffin-NBP3-16970-img0002.jpg "Immunohistochemistry-Paraffin: GLUT9 Antibody [NBP3-16970] - Staining of human cerebral cortex shows low expression as expected.")
![Immunohistochemistry-Paraffin: GLUT9 Antibody [NBP3-16970] - Analysis in human kidney and cerebral cortex tissues using Anti-SLC2A9 antibody. Corresponding SLC2A9 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/GLUT9-Antibody-Immunohistochemistry-Paraffin-NBP3-16970-img0001.jpg)
![Immunohistochemistry-Paraffin: GLUT9 Antibody [NBP3-16970] - Staining of human kidney shows high expression.](http://images.novusbio.com/fullsize/GLUT9-Antibody-Immunohistochemistry-Paraffin-NBP3-16970-img0003.jpg)
![Western Blot: GLUT9 Antibody [NBP3-02991] - Analysis of extracts of various cell lines, using GLUT9 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/GLUT9-Antibody-Western-Blot-NBP3-02991-img0001.jpg "Western Blot: GLUT9 Antibody [NBP3-02991] - Analysis of extracts of various cell lines, using GLUT9 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.")
![Western Blot: GLUT9 Overexpression Lysate (Adult Normal) [NBP2-04185] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for GLUT9.](http://images.novusbio.com/fullsize/GLUT9-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-04185-img0001.jpg "Western Blot: GLUT9 Overexpression Lysate (Adult Normal) [NBP2-04185] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for GLUT9.")