| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-2220SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB600-1384SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-46892SS | |||
| Azide and BSA Free | NBP2-80829 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59800SS | |||
| Azide and BSA Free | NBP2-80830 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-60735SS | |||
| Azide and BSA Free | NBP2-80831 | |||
![Detection of LC3B in mouse ES cell lysates using Rabbit anti- [Catalog # NB100-2220]. LC3 detected at a molecular weight of approximately 17 kDa. Atg5-/- ES cells from Dr. Noboru Mizushima [Mizushima, N. et al. J. Cell Biol. 152 (2001)] Photo courtesy of Dr. Beth Levine, UT SW Medical Center.](http://images.novusbio.com/fullsize/LC3B-Antibody-Western-Blot-NB100-2220-img0047.jpg)
![<b>Biological Strategies Validation. </b>Detection of LC3I and LC3II in mouse cochlea cell line SV-K1 using Rabbit anti- [Catalog # NB100-2220]. Cells were treated with CQ (1 uM), and As2O3 (1 uM) for 24 hrs. Image from verified customer review.](http://images.novusbio.com/fullsize/LC3B-Antibody-Western-Blot-NB100-2220-img0056.jpg)
![Staining of rat brain tissue section. Analysis using Rabbit anti-LC3B Antibody [Catalog # NB100-2220]. Image from verified customer review.](http://images.novusbio.com/fullsize/LC3B-Antibody-Immunohistochemistry-NB100-2220-img0037.jpg)
![FFPE (Formalin-Fixed Paraffin-Embedded) tissue section of mouse brain using 1:200 dilution of Rabbit anti-LC3B antibody [Catalog # NB100-2220]. The specific signal of LC3 was detected using HRP-conjugated secondary antibody with DAB (3, 3 -diaminobenzidine) reagent, and nuclei of cells were counterstained using hematoxylin. This LC3B antibody generated a low to moderate levels of cytoplasmic staining in the glial cells. The neurons depicted a moderate to strong staining for LC3 in their cytoplasm.](http://images.novusbio.com/fullsize/LC3B-Antibody-Immunohistochemistry-Paraffin-NB100-2220-img0048.jpg)
![Analysis using the Biotin conjugate of Rabbit anti-LC3B Antibody [Catalog # NB100-2220]. Staining of brain, cerebral cortex, neurons with cell processes.](http://images.novusbio.com/fullsize/LC3B-Antibody-Immunohistochemistry-NB100-2220-img0053.jpg)
![Analysis of LC3B in HeLa cells using anti-LC3B antibody (red) [Catalog # NB600-1384]. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/LC3B-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NB600-1384-img0033.jpg)
![Analysis of LC3B in treated U87-MG (human glioblastoma astrocytoma) lysates using anti- [Catalog # NB600-1384].](http://images.novusbio.com/fullsize/LC3B-Antibody---BSA-Free-Western-Blot-NB600-1384-img0027.jpg)
![Staining of treated U373-MG (human glioblastoma) cells using anti- [Catalog # NB600-1384].](http://images.novusbio.com/fullsize/LC3B-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NB600-1384-img0030.jpg)
![Analysis of U87MG glioma xenografts using anti- [Catalog # NB600-1384]. Image from verified customer review.](http://images.novusbio.com/fullsize/LC3B-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NB600-1384-img0023.jpg)
![G-TPP induces PINK1 stabilization and kinase activity in HeLa cells(A) G-TPP treatment leads to PINK1 stabilization and pS65-Ub induction in HeLa cells. HeLa cells stably expressing untagged Parkin were treated with 10 µM G-TPP for the indicated times. Western blots were prepared with cell lysates and probed with antibodies against PINK1 and pS65-Ub. GAPDH served as a loading control. (B) pS65-Ub is induced in G-TPP treated cells and co-localizes with EGFP-Parkin and mitochondria. HeLa cells stably expressing EGFP-Parkin (green) were treated with 10 µM G-TPP for the indicated times and fixed. Cells were stained with antibodies against pS65-Ub (red) and the mitochondrial marker TOM20 (cyan). Scale bars correspond to 10 µM. (C) Quantification of Parkin translocation using High Content Imaging. HeLa EGFP-Parkin cells were treated for 4 or 8 h with or without 10 µM G-TPP. CCCP treatment (10 µM for 2 h) was used as a positive control. Cells were fixed, counterstained with Hoechst dye to visualize nuclei, imaged and analyzed using the ratio of cytoplasmic to nuclear EGFP signal [21]. Data was normalized to positive (2 h 10 µM CCCP treatment) and negative (2 h DMSO) controls. G-TPP significantly induced Parkin re-localization to levels similar to or beyond 2 h CCCP treatment. Shown are the mean values of three independent experiments with triplicate wells each ± SEM (one-way ANOVA with Tukey’s posthoc, ***p < 0.0005). Image collected and cropped by CiteAb from the following open publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.22287), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-1384_rabbit-polyclonal-lc3b-antibody-2322024894222.jpg)
![Knockout Validated: LC3B Antibody (1251A) [NBP2-46892] - Western Blot analysis shows lysates of HeLa human cervical epithelial carcinoma parental cell line and LC3B knockout HeLa cell line (KO) untreated (-) or treated (+) with 50 uM Chloroquine for 18 hours. PVDF (Polyvinylidene difluoride) membrane was probed with 2.0 ug/mL of Rabbit Anti-Human/Mouse/Rat LC3B Monoclonal Antibody (1251A) [Catalog # NBP2-46892] followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog# HAF008). A specific band was detected for LC3B at a molecular weight of approximately 15 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. GAPDH is shown as a loading control. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251A-Knockout-Validated-NBP2-46892-img0028.jpg "<b>Genetic Strategies Validation. </b>Knockout Validated: LC3B Antibody (1251A) [NBP2-46892] - Western Blot analysis shows lysates of HeLa human cervical epithelial carcinoma parental cell line and LC3B knockout HeLa cell line (KO) untreated (-) or treated (+) with 50 uM Chloroquine for 18 hours. PVDF (Polyvinylidene difluoride) membrane was probed with 2.0 ug/mL of Rabbit Anti-Human/Mouse/Rat LC3B Monoclonal Antibody (1251A) [Catalog # NBP2-46892] followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog# HAF008). A specific band was detected for LC3B at a molecular weight of approximately 15 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. GAPDH is shown as a loading control. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Knockout Validated: LC3B Antibody (1251A) [NBP2-46892] - LC3B was detected in immersion fixed Chloroquine treated Hela cells (left) but was not detected in LC3B knockout Hela cells (right) using rabbit anti-human LC3B monoclonal antibody (1251A) [Catalog #NBP2-46892] at 0.3 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251A-Knockout-Validated-NBP2-46892-img0036.jpg)
![Western Blot: LC3B Antibody (1251A) [NBP2-46892] - The expression of LC3B in rat tissue. This image was submitted via customer Review.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251A-Western-Blot-NBP2-46892-img0034.jpg)
![<b>Biological Strategies Validation. </b>Flow (Intracellular): LC3B Antibody (1251A) [NBP2-46892] - Jurkat cells were either untreated (A) or treated with 50uM chloroquine for 24 hours (B). An intracellular stain was performed with anti-LC3B (1251A) antibody [Catalog # NBP2-46892] (blue) and a matched isotype control [Catalog # MAB1050] (orange). Cells were fixed with 4% paraformaldehyde, following fixation, cells were permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by rabbit IgG APC-conjugated secondary antibody (F0111, R&D Systems).](http://images.novusbio.com/fullsize/LC3B-Antibody-1251A-Flow-Intracellular-NBP2-46892-img0026.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: LC3B Antibody (1251A) [NBP2-46892] - Effects of PDTC on LC3 on the hippocampus of rats harvested 12 h after CLP. Values of protein data are normalized to actin level and given in relative arbitrary units. LC3-II increased in CLP rats receiving vehicle or PDTC compared with those in sham-operated rats. PDTC + CLP rats showed an increase in LC3-II compared with vehicle-treated rats. Data were expressed as mean +/- SEM, n = 6/group. *P < 0.05 vs sham-operated group. # P < 0.05 vs 12 h or Veh + CLP group. PDTC pyrrolidine dithiocarbamate, LC-3 microtubule-associated protein light chain-3, CLP cecal ligation and puncture. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26067996) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251A-Western-Blot-NBP2-46892-img0038.jpg)
![Immunocytochemistry/Immunofluorescence: LC3B Antibody (1251A) [NBP2-46892] - HeLa cells were treated with Chloroquine for 24 hours prior to fixation, permeabilization and incubation with anti- [Catalog # NBP2-46892] and anti tubulin (NB100-690) antibodies. Image enlargement shows the accumulation of LC3 (green) on autophagosomes in response to Chloroquine treatment. Tubulin staining is shown in red and DNA is counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/LC3B-Antibody-1251A-Immunocytochemistry-Immunofluorescence-NBP2-46892-img0032.jpg)
![Immunohistochemistry-Paraffin: LC3B Antibody (1251A) [NBP2-46892] - IHC (Immunohistochemical) analysis of a formalin fixed and paraffin embedded tissue section of normal mouse brain using rabbit monoclonal LC3B (1251A) antibody [Catalog # NBP2-46892] at 1:100 dilution with HRP-DAB detection. The antibody generated a weak diffused cytoplasmic staining in most of the cells but some cells, especially within empty areas on the section, showed punctate signal also which signifies the presence of autophagy in those areas.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251A-Immunohistochemistry-Paraffin-NBP2-46892-img0033.jpg)
![Flow Cytometry: LC3B Antibody (1251A) [NBP2-46892] - HeLa cells untreated or treated with 50 uM chloroquine for 24h. Image from the Alexa Fluor 405 version of this antibody.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251A-Flow-Cytometry-NBP2-46892-img0037.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: LC3B Antibody (1251A) [NBP2-46892] - Analysis shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with Chloroquine. PVDF membrane was probed with 0.2 ug/mL rabbit anti-LC3B monoclonal Antibody (1251A) (Catalog # NBP2-46892, Novus Biologicals), followed by 1:2000 dilution of goat anti-rabbit IgG secondary antibody. A specific band was detected for LC3B at a molecular weight of approximately 15 kDa in CQ treated NIH-3T3 and PC12 cell lines.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251A-Western-Blot-NBP2-46892-img0009.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: LC3B Antibody (1251A) [NBP2-46892] - Western Blot analysis using HeLa cells treated with (+) or without (-) Chloriquine (CQ). A specific band was detected for LC3B at a molecular weight of approximately 15 kDa.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251A-Western-Blot-NBP2-46892-img0010.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: LC3B Antibody (1251A) [NBP2-46892] - Western Blot image of monoclonal anti-LC3B Antibody (Clone 1251D) [Catalog # NBP2-46892]. HeLa and Neuro2A cells were treated with or without 50 uM chloroquine for 24 hours as indicated. Whole cell protein was then separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane for 30 min and blocked in 5% non-fat milk in TBST (Tris-buffered saline, 0.1% Tween 20). The membrane was probed with 2 ug/ml anti-LC3B Antibody in 1% milk, and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Note the accumulation of LC3B II upon chloroquine treatment. Bands for LC3 were detected at a molecular weight of approximately 15 kDa in treated HeLA cells, and both treated and untreated Neuro2A cells.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251A-Western-Blot-NBP2-46892-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: LC3B Antibody (1251A) [NBP2-46892] - HeLa cells were treated with 50 uM Chloroquine for 24 hour prior to fixation in 10% buffered formalin for 10 min. Cells were permeabilized in 0.1% Triton X-100 and incubated with 20 ug/ml anti- [Catalog # NBP2-46892] and 1:500 anti-tubulin [Catalog # NB100-690] for 1 h at room temperature. LC3B reactivity (green) was detected with ant-rabbit Dylight 488 and tubulin (red) with anti-mouse Dylight 550. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/LC3B-Antibody-1251A-Immunocytochemistry-Immunofluorescence-NBP2-46892-img0030.jpg)
![Flow (Intracellular): LC3B Antibody (1251A) [NBP2-46892] - HeLa human cervical epithelial carcimoa cell line either treated with 50 uM chloroquine for 24 hours (filled histogram) or untreated (open histogram) was stained with Rabbit anti-Human LC3B Alexa Fluor 647 conjugated monoclonal antiobdy (1251A) [Catalog # NBP2-46892AF647]. To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation and Permeabilization Buffer Kit (FC012-NOV).](http://images.novusbio.com/fullsize/LC3B-Antibody-1251A-Flow-Intracellular-NBP2-46892-img0025.jpg)
![Immunoprecipitation: LC3B Antibody (1251A) [NBP2-46892] - Western blot analysis of LC3 immunoprecipitation. 10 ug of monoclonal anti-LC3B antibody (clone 1251A) [Catalog # NBP2-46892] was used to immunoprecipitate LC3 from 200 ug of total protein from HeLa cells treated with or without 50 uM chloroquine for 24 h. Antibody-protein was captured by magnetic protein A/G beads, washed and subjected to SDS-PAGE on a 4-15% gel. Protein was transferred to 0.2 um PVDF membrane and probed with 2 ug/ml anti-LC3B (NB100-2220) and detected with an anti-rabbit HRP conjugated secondary antibody using chemiluminescence. The detection of LC3 at a molecular weight of approximately 12 kDa and the antibody heavy chain (Ab Hc) at a molecular weight of approximately 50 kDa is indicated.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251A-Immunoprecipitation-NBP2-46892-img0031.jpg)
![Knockout Validated: LC3 Antibody Pack [NB910-40435] - Lysates of HeLa parental cell line and LC3B knockout HeLa cell line (KO) untreated (-) or treated (+) with 50 uM Chloroquine for 18 hours. PVDF (Polyvinylidene difluoride) membrane was probed with 0.5 ug/mL of Rabbit Anti-LC3B Polyclonal Antibody (Catalog # NB100-2220) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog# HAF008). A specific band was detected for LC3B at a molecular weight of approximately 15 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. GAPDH is shown as a loading control. This experiment was conducted under reducing conditions. LC3B Antibody [NB100-2220]](http://images.novusbio.com/fullsize/LC3-Antibody-Pack-Knockout-Validated-NB910-40435-img0013.jpg "<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Knockout Validated: LC3 Antibody Pack [NB910-40435] - Lysates of HeLa parental cell line and LC3B knockout HeLa cell line (KO) untreated (-) or treated (+) with 50 uM Chloroquine for 18 hours. PVDF (Polyvinylidene difluoride) membrane was probed with 0.5 ug/mL of Rabbit Anti-LC3B Polyclonal Antibody (Catalog # NB100-2220) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog# HAF008). A specific band was detected for LC3B at a molecular weight of approximately 15 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. GAPDH is shown as a loading control. This experiment was conducted under reducing conditions. LC3B Antibody [NB100-2220]")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Immunohistochemistry: LC3 Antibody Pack [NB910-40435] - Rapamycin increases autophagy in brains of PDAPP mice. Representative epifluorescent (c200x) image of hippocampal CA1 in control- and rapamycin-fed transgenic PDAPP mice stained with an anti-LC3 antibody. An increase in LC3-immunoreactive puncta was observed in CA1 projections of transgenic PDAPP mice following rapamycin administration. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0009979) licensed under a CC-BY license. LC3A Antibody [NB100-2331]](http://images.novusbio.com/fullsize/LC3-Antibody-Pack-Immunohistochemistry-NB910-40435-img0009.jpg)
![<b>Genetic Strategies Validation. </b>Knockout Validated: LC3 Antibody Pack [NB910-40435] - LC3B was detected in immersion fixed Cloroquine treated HeLa cells (left) but was not detected in LC3B knockout HeLa cells (right) using rabbit anti-human LC3B polyclonal antibody (Catalog #NB100-2220) at 0.3 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights(TM) 557-conjugated anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/LC3-Antibody-Pack-Knockout-Validated-NB910-40435-img0014.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: LC3 Antibody Pack [NB910-40435] - High autophagosome concentration is consumed during early immortalized human mesenchymal stem cell differentiation. Immortalized human mesenchymal stem cells were differentiated under osteogenic conditions (see Materials and methods) and assayed for changes in LC3I and LC3II during a 72-hour window. Cells were differentiated under standard conditions (top) or with addition of 5 uM rapamycin (middle) or 5 nM bafilomycin (bottom) for the first 3 hours of differentiation to modulate autophagy. Immunoblots were performed for LC3 at the indicated time points to assess autophagosome degradation via relative changes in LC3II (lower band; 17 kDa). Studies were repeated three times with similar trends seen consistently. Image collected and cropped by CiteAb from the following publication (//stemcellres.com/content/5/6/140), licensed under a CC-BY license. LC3A Antibody [NB100-2331]](http://images.novusbio.com/fullsize/LC3-Antibody-Pack-Western-Blot-NB910-40435-img0011.jpg)
![Immunohistochemistry: LC3 Antibody Pack [NB910-40435] - Localization of LC3 by immunohistochemical method. The immunohistochemical staining shows that LC3, CRF, and HIF-1alpha have an overlapping localization in villous and extravillous trophoblast. d: decidua; v: villi; DeVe: decidual vessel. Original magnification 40x. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/bmri/2013/689768/) licensed under a CC-BY license. LC3B Antibody [NB100-2220]](http://images.novusbio.com/fullsize/LC3-Antibody-Pack-Immunohistochemistry-NB910-40435-img0015.jpg)
![Immunohistochemistry: LC3 Antibody Pack [NB910-40435] - Brain, Cerebral Cortex, Cell Processes in Gray Matter 40x.](http://images.novusbio.com/fullsize/LC3-Antibody-Pack-Immunohistochemistry-NB910-40435-img0008.jpg)
![Immunohistochemistry: LC3 Antibody Pack [NB910-40435] - Analysis in PFA fixed NIH/3T3 cells using anti-LC3A antibody. Image from verified customer review. LC3A Antibody [NB100-2331]](http://images.novusbio.com/fullsize/LC3-Antibody-Pack-Immunohistochemistry-NB910-40435-img0012.jpg)
![Western Blot: LC3 Antibody Pack [NB910-40752] - LC3/MAP1LC3A Antibody Pack [NB910-40752] - Detection of LC3B in treated U87-MG (human glioblastoma astrocytoma) lysates using NB600-1384.](http://images.novusbio.com/fullsize/LC3-MAP1LC3A-Antibody-Pack-Western-Blot-NB910-40752-img0026.jpg "<b>Biological Strategies Validation. </b>Western Blot: LC3 Antibody Pack [NB910-40752] - LC3/MAP1LC3A Antibody Pack [NB910-40752] - Detection of LC3B in treated U87-MG (human glioblastoma astrocytoma) lysates using NB600-1384.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: LC3 Antibody Pack [NB910-40752] - LC3/MAP1LC3A Antibody Pack [NB910-40752] - Immunofluorescent staining of treated U373-MG cells using NB600-1384. The nuclei were stained with DAPI.](http://images.novusbio.com/fullsize/LC3-MAP1LC3A-Antibody-Pack-Immunocytochemistry-Immunofluorescence-NB910-40752-img0017.jpg)
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Knockout Validated: LC3 Antibody Pack [NB910-40752] - Lysates of HeLa parental cell line and LC3B knockout HeLa cell line (KO) untreated (-) or treated (+) with 50 uM Chloroquine for 18 hours. PVDF (Polyvinylidene difluoride) membrane was probed with 0.5 ug/mL of Rabbit Anti-LC3B Polyclonal Antibody (Catalog # NB100-2220) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog# HAF008). A specific band was detected for LC3B at a molecular weight of approximately 15 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. GAPDH is shown as a loading control. This experiment was conducted under reducing conditions. LC3B Antibody [NB100-2220]](http://images.novusbio.com/fullsize/LC3-Antibody-Pack-Knockout-Validated-NB910-40752-img0027.jpg)
![Immunohistochemistry: LC3 Antibody Pack [NB910-40752] - Autophagosomes accumulate in neurons of the VH gray matter at day 1 after SCI. Representative images of IHC staining for LC3 (green) and neuronal marker NeuN (red) in VH of gray matter from sham and SCI animals. Stronger co-localization between NeuN and LC3 is apparent at day 1 after SCI. Scale bar is 20 um. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/cddis2014527) licensed under a CC-BY license. LC3B Antibody [NB100-2220]](http://images.novusbio.com/fullsize/LC3-Antibody-Pack-Immunohistochemistry-NB910-40752-img0029.jpg)
![Immunohistochemistry: LC3 Antibody Pack [NB910-40752] - Analysis in mouse renal tissue. Image from verifed customer review. LC3A Antibody [NB100-2331]](http://images.novusbio.com/fullsize/LC3-Antibody-Pack-Immunohistochemistry-NB910-40752-img0030.jpg)
![Western Blot: LC3/MAP1LC3A Antibody Pack [NB910-40752] - Detection of autophagic LC3 in mouse ES cell lysate using NB 100-2331. The atg5-/- lane (ES cells, cultured to form embryonic bodies, that are deficient in conversion of LC3-1 to LC3-11) demonstrates the specificity of NB 100-2331, as there is no detection of LC3-11. Photo courtesy of Dr. Beth Levine, UT Southwestern Medical Center.](http://images.novusbio.com/fullsize/LC3-MAP1LC3A-Antibody-Pack-Western-Blot-NB910-40752-img0025.jpg)
![Immunohistochemistry: LC3/MAP1LC3A Antibody Pack [NB910-40752] - Staining of treated U373-MG (human glioblastoma) cells using NB600-1384.](http://images.novusbio.com/fullsize/LC3-MAP1LC3A-Antibody-Pack-Immunohistochemistry-NB910-40752-img0021.jpg)
![Knockout Validated: LC3B Antibody (1251D) [NBP2-59800] - HeLa human cervical epithelial carcinoma parental cell line and LC3B knockout HeLa cell line (KO) untreated (-) or treated (+) with 50 uM Chloroquine for 18 hrs. PVDF membrane was probed with 0.1 ug/mL of Rabbit Anti-LC3B Monoclonal Antibody (1251D) [Catalog# NBP2-59800] followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog# HAF008). A specific band was detected for LC3B at approximately 15 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251D-Knockout-Validated-NBP2-59800-img0005.jpg "<b>Genetic Strategies Validation. </b>Knockout Validated: LC3B Antibody (1251D) [NBP2-59800] - HeLa human cervical epithelial carcinoma parental cell line and LC3B knockout HeLa cell line (KO) untreated (-) or treated (+) with 50 uM Chloroquine for 18 hrs. PVDF membrane was probed with 0.1 ug/mL of Rabbit Anti-LC3B Monoclonal Antibody (1251D) [Catalog# NBP2-59800] followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog# HAF008). A specific band was detected for LC3B at approximately 15 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions.")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: LC3B Antibody (1251D) [NBP2-59800] - Total protein from HeLa and Neuro2A cells treated with or without 50 uM chloroquine for 24 hrs was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF (Polyvinylidene difluoride) membrane and blocked in 5% non-fat milk in TBST (Tris-buffered saline, 0.1% Tween 20). The membrane was probed with 2.0 ug/ml anti-LC3B Antibody (1251d) [Catalog # NBP2-59800] in blocking buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Note the detection of LC3I and LC3II at a molecular weight of approximately 15 kDa.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251D-Western-Blot-NBP2-59800-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: LC3B Antibody (1251D) [NBP2-59800] - HeLa cells were treated with 50uM CQ (Chloroquine) overnight, fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-LC3B (1251d) [Catalog # NBP2-59800] at 5 ug/ml overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251D-Immunocytochemistry-Immunofluorescence-NBP2-59800-img0003.jpg)
![Immunohistochemistry-Paraffin: LC3B Antibody (1251D) [NBP2-59800] - Analysis of a FFPE (Formalin-Fixed Paraffin-Embedded) tissue section of mouse brain using anti-LC3B antibody (clone 1251D) [Catalog # NBP2-59800] at 5ug/ml concentration (1:200 dilution). The primary antibody binding to LC3 in cells was detected using HRP conjugated anti-Rabbit secondary antibody with DAB (3,3'Diaminobenzidine) reagent, and the sections were further counterstained with hematoxylin for labeling cellular nuclei. This LC3 antibody generated a diffused staining in all cell types (except the endothelial cells of blood vessels) and the signal was strongest in a subset of neuronal cells.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251D-Immunohistochemistry-Paraffin-NBP2-59800-img0023.jpg)
![<b>Biological Strategies Validation. </b>Flow (Intracellular): LC3B Antibody (1251D) [NBP2-59800] - HeLa cells were either (A) untreated or (B) treated with 50uM chloroquine for 24 hrs. An intracellular stain was performed with anti-LC3B (1251D) antibody [Catalog # NBP2-59800] (blue) and a matched isotype control [Catalog # MAB1050] (orange). Cells were fixed with 4% paraformaldehyde, following fixation, cells were permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by rabbit IgG APC-conjugated secondary antibody (F0111, R&D Systems).](http://images.novusbio.com/fullsize/LC3B-Antibody-1251D-Flow-Intracellular-NBP2-59800-img0022.jpg)
![<b>Biological Strategies Validation. </b>Simple Western: LC3B Antibody (1251D) [NBP2-59800] - Lane view shows lysates of NIH-3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 50 uM Chloroquine for 18 hrs, loaded at 0.2 mg/ml. A specific band was detected for LC3B at approximately 17 kDa (as indicated) using 5 ug/mL of Rabbit Anti-Human LC3B Monoclonal Antibody (1251D) [Catalog# NBP2-59800]. This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251D-Simple-Western-NBP2-59800-img0021.jpg)
![Knockout Validated: LC3 Antibody Pack [NBP2-59703] - LC3B was detected in immersion fixed Chloroquine treated HeLa cells (left) but was not detected in LC3B knockout HeLa cells (right) using rabbit anti-human LC3B monoclonal antibody (1251A) [Catalog #NBP2-46892] at 0.3 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights(TM) 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/LC3-Antibody-Pack-Knockout-Validated-NBP2-59703-img0004.jpg "<b>Genetic Strategies Validation. </b>Knockout Validated: LC3 Antibody Pack [NBP2-59703] - LC3B was detected in immersion fixed Chloroquine treated HeLa cells (left) but was not detected in LC3B knockout HeLa cells (right) using rabbit anti-human LC3B monoclonal antibody (1251A) [Catalog #NBP2-46892] at 0.3 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights(TM) 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.")
![<b>Genetic Strategies Validation. </b>Knockout Validated: LC3 Antibody Pack [NBP2-59703] - LC3B was detected in immersion fixed Cloroquine treated HeLa cells (left) but was not detected in LC3B knockout HeLa cells (right) using rabbit anti-human LC3B polyclonal antibody (Catalog #NB100-2220) at 0.3 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights(TM) 557-conjugated anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/LC3-Antibody-Pack-Knockout-Validated-NBP2-59703-img0002.jpg)
![Immunohistochemistry: LC3 Antibody Pack [NBP2-59703] - IHC (Immunohistochemical) analysis of a formalin fixed and paraffin embedded tissue section of normal mouse brain using rabbit monoclonal LC3B (1251A) antibody [Catalog # NBP2-46892] at 1:100 dilution with HRP-DAB detection. The antibody generated a weak diffused cytoplasmic staining in most of the cells but some cells, especially within empty areas on the section, showed punctate signal also which signifies the presence of autophagy in those areas.](http://images.novusbio.com/fullsize/LC3-Antibody-Pack-Immunohistochemistry-NBP2-59703-img0005.jpg)
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Knockout Validated: LC3 Antibody Pack [NBP2-59703] - Lysates of HeLa parental cell line and LC3B knockout HeLa cell line (KO) untreated (-) or treated (+) with 50 uM Chloroquine for 18 hours. PVDF (Polyvinylidene difluoride) membrane was probed with 0.5 ug/mL of Rabbit Anti-LC3B Polyclonal Antibody (Catalog # NB100-2220) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog# HAF008). A specific band was detected for LC3B at a molecular weight of approximately 15 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. GAPDH is shown as a loading control. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/LC3-Antibody-Pack-Knockout-Validated-NBP2-59703-img0001.jpg)
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Knockout Validated: LC3 Antibody Pack [NBP2-59703] - Western Blot analysis shows lysates of HeLa human cervical epithelial carcinoma parental cell line and LC3B knockout HeLa cell line (KO) untreated (-) or treated (+) with 50 uM Chloroquine for 18 hours. PVDF (Polyvinylidene difluoride) membrane was probed with 2.0 ug/mL of Rabbit Anti-Human/Mouse/Rat LC3B Monoclonal Antibody (1251A) [Catalog # NBP2-46892] followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog# HAF008). A specific band was detected for LC3B at a molecular weight of approximately 15 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. GAPDH is shown as a loading control. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/LC3-Antibody-Pack-Knockout-Validated-NBP2-59703-img0003.jpg)
![Knockout Validated: LC3B Antibody (1251B) [NBP2-60735] - Western blot analysis of HeLa human cervical epithelial carcinoma parental cell line and LC3B knockout HeLa cell line (KO) untreated (-) or treated (+) with 50uM Chloroquine for 18 hours. PVDF (polyvinylidene difluoride) membrane was probed with 0.1 ug/mL of anti-LC3B monoclonal antibody (Catalog # NBP2-60735) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for LC3B at approximately 15 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251B-Knockout-Validated-NBP2-60735-img0003.jpg "<b>Genetic Strategies Validation. </b>Knockout Validated: LC3B Antibody (1251B) [NBP2-60735] - Western blot analysis of HeLa human cervical epithelial carcinoma parental cell line and LC3B knockout HeLa cell line (KO) untreated (-) or treated (+) with 50uM Chloroquine for 18 hours. PVDF (polyvinylidene difluoride) membrane was probed with 0.1 ug/mL of anti-LC3B monoclonal antibody (Catalog # NBP2-60735) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for LC3B at approximately 15 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control.")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Simple Western: LC3B Antibody (1251B) [NBP2-60735] - Lane view shows lysates of NIH-3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 50uM Chloroquine for 18 hours, loaded at 0.2 mg/mL. A specific band was detected for LC3B at approximately 17 kDa (as indicated) using 5 ug/mL of Rabbit Anti-Human/Mouse/Rat anti-LC3B Monoclonal Antibody (1251B) [Catalog # NBP2-60735]. This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251B-Simple-Western-NBP2-60735-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: LC3B Antibody (1251B) [NBP2-60735] - HeLa cells were treated with 50uM CQ (Chloroquine) overnight, fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti- [Catalog # NBP2-60735] at 2 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251B-Immunocytochemistry-Immunofluorescence-NBP2-60735-img0007.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: LC3B Antibody (1251B) [NBP2-60735] - Total protein from HeLa and Neuro2A cells treated with or without 50 uM chloroquine (CQ) for 24 hours was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF (Polyvinylidene difluoride) membrane and blocked in 5% non-fat milk in TBST (Tris-buffered saline, 0.1% Tween 20). The membrane was probed with 12.0 ug/ml anti-LC3B Antibody (1251B) [Catalog # NBP2-60735] in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251B-Western-Blot-NBP2-60735-img0010.jpg)
![Immunohistochemistry-Paraffin: LC3B Antibody (1251B) [NBP2-60735] - Analysis of a FFPE (Formalin-Fixed Paraffin-Embedded) tissue section of mouse brain using anti-LC3B antibody (clone 1251B) [Catalog # NBP2-60735] at 5ug/ml concentration (1:200 dilution). The primary antibody binding to LC3 in cells was detected using HRP conjugated anti-Rabbit secondary antibody with DAB (3,3'Diaminobenzidine) reagent, and the sections were further counterstained with hematoxylin for labeling cellular nuclei. This LC3 antibody generated a diffused staining in all cell types (except the endothelial cells of blood vessels) and the signal was strongest in a subset of neuronal cells. A few cells depicted LC3 puncta formation/dotted staining in apparently autophagic cells.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251B-Immunohistochemistry-Paraffin-NBP2-60735-img0011.jpg)
![<b>Biological Strategies Validation. </b>Flow (Intracellular): LC3B Antibody (1251B) [NBP2-60735] - HeLa cells were either (A) untreated or (B) treated with 50uM chloroquine for 24 hours. An intracellular stain was performed with anti-LC3B (1251B) antibody [Catalog # NBP2-60735] (blue) and a matched isotype control [Catalog # MAB1050] (orange). Cells were fixed with 4% paraformaldehyde, following fixation, cells were permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by rabbit IgG APC-conjugated secondary antibody (F0111, R&D Systems).](http://images.novusbio.com/fullsize/LC3B-Antibody-1251B-Flow-Intracellular-NBP2-60735-img0009.jpg)
![Western Blot: LC3B Antibody (7D11) [NBP3-26154] - Positive Western Blot detected in: HepG2 whole cell lysate, U87 whole cell lysate, RAW264.7 whole cell lysate, U251 whole cell lysate, Hela whole cell lysate. All lanes: LC3B Antibody at 1: 500 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 15 kDa Observed band size: 15 kDa](http://images.novusbio.com/fullsize/nbp3-26154_rabbit-lc3b-mab-7d11-28220241144268.jpg "Western Blot: LC3B Antibody (7D11) [NBP3-26154] - Positive Western Blot detected in: HepG2 whole cell lysate, U87 whole cell lysate, RAW264.7 whole cell lysate, U251 whole cell lysate, Hela whole cell lysate. All lanes: LC3B Antibody at 1: 500 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 15 kDa Observed band size: 15 kDa")
![Flow Cytometry: LC3B Antibody (7D11) [NBP3-26154] - Overlay Peak curve showing Hela cells stained with LC3B Antibody (7D11) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26154_rabbit-lc3b-mab-7d11-282202414183621.jpg)
