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NBP1-48308

PKM2 Antibody - BSA Free

(3 Reviews)

(17 Publications)

0.1 ml

NBP1-48308

Immunocytochemistry/Immunofluorescence: PKM2 Antibody - BSA Free [NBP1-48308] - Analysis of PKM2 in frozen sections of rat glioblastoma tissue. Image courtsey of anonymous customer review.

Immunohistochemistry: PKM2 Antibody - BSA Free [NBP1-48308] - Staining of PKM2 in mouse liver tissue.

Flow Cytometry: PKM2 Antibody - BSA Free [NBP1-48308] - An intracellular stain was performed on Neuro2a cells with PKM2 Antibody NBP1-48308AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

Western Blot: PKM2 Antibody - BSA Free [NBP1-48308] - Analysis of HeLa lysates using NBP1-48308. Image courtesy of Gregg Semenza, (PMID: 21620138).

Western Blot: PKM2 Antibody - BSA Free [NBP1-48308] - Analysis of PKM2 in MCF7 whole cell lysates.

Western Blot: PKM2 Antibody - BSA Free [NBP1-48308] - Expression of PKM1 and PKM2 in clinical colorectal cancer samples. The protein expression of PKM1 and PKM2 in clinical specimens of cancer tumor (T) and the adjacent normal tissues (N) is shown. PKM1 and PKM2 were detected by Western blotting in under the same experimental conditions at the same time. The full-length blots are presented in Supplementary Figure S3b. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep08647) licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: PKM2 Antibody - BSA Free [NBP1-48308] - Analysis of PKM2 in HeLa cells.

Immunohistochemistry: PKM2 Antibody - BSA Free [NBP1-48308] - Immunohistochemical staining of normal colon tissue adjacent to tumor tissue of case 10. Results of staining with anti-PKM2 is shown. The boxed regions are enlarged in the images below. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25721733/) licensed under a CC-BY license.

Flow Cytometry: PKM2 Antibody - BSA Free [NBP1-48308] - An intracellular stain was performed on HeLa cells with PKM2 Antibody NBP1-48308AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

Simple Western: PKM2 Antibody - BSA Free [NBP1-48308] - Lane view shows a specific band for PKM2 in 0.1 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation system. * Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Identification of plasma membrane calcium ATPase isoform PMCA4b as a renalase binding protein.A, HK-2 cells incubated with either labeled RP-Scr220 or RP-220, biotin-labeled proteins purified using streptavidin column, separated by SDS-PAGE and visualized by western blot using streptavidin-HRP; * = regions evaluated by mass spectrometry in samples labeled with either RP-Scr220 or RP-220; # = RP-220 band containing the plasma membrane calcium ATPase isoform PMCA4b. B, Endogenous expression of PMCA4b in HK-2 cells, western immunoblot using isoform specific monoclonal; CCL-119: human leukemic cell line; thyroid tumor = human thyroid tumor cell line (ATCC, CRL-1803) 10 μg protein loaded in each lane. C, co-immunolocalization of PMCA4b and renalase in HK-2 cells, images acquired using a Zeiss laser scanning confocal microscope, scale bar = 9 μm; arrow = plasma membrane. D, Co-Immunoprecipitation of PMCA4b and renalase from HK-2 cell lysates; renalase-Ab-beads = renalase antibody coated beads; PMCA4b-Ab-beads = PMCA4b antibody coated beads. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/25906147), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

KLF4 ablation leads to abnormal proliferation and differentiation in small intestinal epithelium.(A) Small intestine from Klf4−/− mice induced by tamoxifen for different time endurances were stained by H&E and PAS, and also immunohistochemistry staining was performed with anti-Ki67, anti-Lysozyme, anti-DCAMKL-1, and anti-PCNA antibodies respectively. (B) Statistic analysis of IHC staining results from (A). (*, P<0.05) (C) IHC staining from (A) in higher magnification of highlighted frames. Bottom panel: IHC staining with ZO-1 antibody in one-month knockout intestine tissue. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/22384261), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt, Bv

Species Glossary

Reactivity: Hu, Mu, Rt, Bv

Applications:

, , , , , , ,

Applications: , , , , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-48308AF350
Alexa Fluor 405	NBP1-48308AF405
Alexa Fluor 488	NBP1-48308AF488
Alexa Fluor 532	NBP1-48308AF532
Alexa Fluor 594	NBP1-48308AF594
Alexa Fluor 647	NBP1-48308AF647
Alexa Fluor 700	NBP1-48308AF700
Alexa Fluor 750	NBP1-48308AF750
Allophycocyanin	NBP1-48308APC
Biotin	NBP1-48308B
DyLight 350	NBP1-48308UV
DyLight 405	NBP1-48308V
DyLight 488	NBP1-48308G
DyLight 550	NBP1-48308R
DyLight 594	NBP1-48308DL594
DyLight 650	NBP1-48308C
DyLight 680	NBP1-48308FR
DyLight 755	NBP1-48308IR
FITC	NBP1-48308F
HRP	NBP1-48308H
Janelia Fluor 549	NBP1-48308JF549
Janelia Fluor 646	NBP1-48308JF646
PE	NBP1-48308PE
PerCP	NBP1-48308PCP
mFluor Violet 450 SE	NBP1-48308MFV450
mFluor Violet 500 SE	NBP1-48308MFV500
mFluor Violet 610 SE	NBP1-48308MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-48308SS

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AF7244

PKM2 Antibody [Unconjugated]

(3 Publications)

100 μg

AF7244

Western blot shows lysates of A549 human lung carcinoma cell line, KG-1 human acute myelogenous leukemia cell line, BaF3 mouse pro-B cell line, and NRK rat normal kidney cell line. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human/Mouse/Rat PKM1/2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7244) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band was detected for PKM2 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

PKM2 was detected in immersion fixed Wi-38 human lung fibroblast cell line using Sheep Anti-Human/Mouse/Rat PKM1/2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7244) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

PKM2 was detected in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line using Sheep Anti-Human/Mouse/Rat PKM1/2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7244) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

PKM2 was detected in perfusion fixed frozen sections of mouse testis using Sheep Anti-Human/Mouse/Rat PKM1/2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7244) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to Sertoli cells in testis. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

PKM2 was detected in perfusion fixed frozen sections of rat testis using Sheep Anti-Human/Mouse/Rat PKM1/2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7244) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to Sertoli cells in testis. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Simple Western lane view shows lysates of A549 human lung carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for PKM2 at approximately 60 kDa (as indicated) using 2 µg/mL of Sheep Anti-Human/Mouse/Rat PKM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7244) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.

<b>Genetic Strategies Validation. </b>Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and PKM2 knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat PKM1/2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7244) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band was detected for PKM2 at approximately 60 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western blot shows lysates of U‑87 MG human glioblastoma/astrocytoma cell line, A549 human lung carcinoma cell line, HeLa human cervical epithelial carcinoma cell line, human skeletal muscle. PVDF membrane was probed with 0.25 µg/mL of Sheep Anti-Human/Mouse/Rat PKM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7244) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band was detected for PKM2 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , ,

Applications: , , , ,

Host:

Sheep Polyclonal

Host: Sheep Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF7244-SP

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MAB7244

PKM2 Antibody (937801) [Unconjugated]

(1 Publication)

100 μg

MAB7244

PKM2 was detected in immersion fixed WI-38 human lung fibroblast cell line using Mouse Anti-Human/Rat PKM1/2 Monoclonal Antibody (Catalog # MAB7244) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

PKM2 was detected in immersion fixed paraffin-embedded sections of human prostate using Mouse Anti-Human/Rat PKM1/2 Monoclonal Antibody (Catalog # MAB7244) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cell cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

PKM2 was detected in perfusion fixed frozen sections of rat skeletal muscle using Mouse Anti-Human/Rat PKM1/2 Monoclonal Antibody (Catalog # MAB7244) at 8 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to epithelial cell cytoplasm. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for PKM2 at approximately 61 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human/Rat PKM2 Monoclonal Antibody (Catalog # MAB7244) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

<b>Genetic Strategies Validation. </b>Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and PKM2 knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Rat PKM1/2 Monoclonal Antibody (Catalog # MAB7244) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for PKM2 at approximately 60 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (AF5718) is shown as a loading control.. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

<b>Genetic Strategies Validation. </b>PKM2 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line but is not detected in PKM2 knockout (KO) HeLa Human Cell Line cell line using Mouse Anti-Human/Rat PKM1/2 Monoclonal Antibody (Catalog # MAB7244) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Mouse IgG Secondary Antibody (green; NL009) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Western blot shows lysates of U‑87 MG human glioblastoma/astrocytoma cell line, A549 human lung carcinoma cell line, HeLa human cervical epithelial carcinoma cell line, human skeletal muscle. PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Human/Rat PKM2 Monoclonal Antibody (Catalog # MAB7244) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for PKM2 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Reactivity:

Hu, Rt

Species Glossary

Reactivity: Hu, Rt

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB7244-SP

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NBP1-82487

PKM2 Antibody

(3 Publications)

0.1 ml

NBP1-82487

Western Blot: PKM2 Antibody [NBP1-82487] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Immunocytochemistry/Immunofluorescence: PKM2 Antibody [NBP1-82487] - Staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: PKM2 Antibody [NBP1-82487] - Staining of human fallopian tube shows moderate cytoplasmic and membranous positivity in glandular cells.

<b>Genetic Strategies Validation. </b>Western Blot: PKM2 Antibody [NBP1-82487] - Analysis in A-549 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-PKM antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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7244-PK

Recombinant Human PKM2 Protein, CF

(1 Publication)

20 μg

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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NB600-1447

PKM2 Antibody

(1 Publication)

0.2 ml

Reactivity:

Rb

Species Glossary

Reactivity: Rb

Applications:

, , ,

Applications: , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Biotin	NB120-20589-0.1mg

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