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PKM2 Antibody

Images

 
Western Blot: PKM2 Antibody [NBP1-82487] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunocytochemistry/ Immunofluorescence: PKM2 Antibody [NBP1-82487] - Staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: PKM2 Antibody [NBP1-82487] - Staining of human fallopian tube shows moderate cytoplasmic and membranous positivity in glandular cells.
Genetic Strategies: Western Blot: PKM2 Antibody [NBP1-82487] - Analysis in A-549 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-PKM antibody. Remaining relative intensity ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

   

Order Details

PKM2 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: TATESFASDPILYRPVAVALDTKGPEIRTGLIKGSGTAEVELKKGATLKITLDNAYMEKCDENILWLDYKNICKVVEVGSKIYVDDGLISLQVKQKGADFLVTEVENGGSL
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PKM
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Knockdown Validated
  • Western Blot 0.04-0.4 ug/ml
Application Notes
IHC reported in scientific literature (PMID: 21738817). For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
PKM2 Protein (NBP1-82487PEP)
Publications
Read Publications using
NBP1-82487 in the following applications:

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 21738817).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for PKM2 Antibody

  • CTHBP
  • Cytosolic thyroid hormone-binding protein
  • MGC3932
  • OIP3
  • OIP-3
  • OIP3EC 2.7.1.40
  • Opa-interacting protein 3
  • p58
  • PK, muscle type
  • PK2
  • PK3
  • PK3PKM
  • PKM2
  • Pyruvate kinase 2/3
  • pyruvate kinase isozymes M1/M2
  • Pyruvate kinase muscle isozyme
  • pyruvate kinase, muscle
  • TCB
  • THBP1
  • THBP1p58
  • Thyroid hormone-binding protein 1
  • thyroid hormone-binding protein, cytosolic
  • Tumor M2-PK

Background

PKM2 is encoded by this gene is a pyruvate kinase that catalyzes the production of phosphoenolpyruvate from pyruvate and ATP. This protein has been shown to interact with thyroid hormone, and thus may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Three alternatively spliced transcript variants encoding two distinct isoforms have been reported.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PKM2 Antibody (NBP1-82487)(3)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PKM2 Antibody (NBP1-82487) (0)

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Secondary Antibodies

 

Isotype Controls

Additional PKM2 Products

Research Areas for PKM2 Antibody (NBP1-82487)

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Blogs on PKM2.

MAPK3/ERK1 - A signal transduction pathway with roles in development and disease
Mitogen-activated protein kinases (MAPKs) are important signaling proteins needed to transmit and relay extracellular stimuli and to illicit intracellular responses (1). The MAPK family of proteins are serine/threonine kinases that are able to phos...  Read full blog post.

5 Stars for the PKM2 Antibody
Novus' Pyruvate Kinase M2 antibody (cat # NBP1-48308) has received some glowing product reviews and customer feedback lately. One satisfied customer wrote, "The best thing about this antibody is that it works well for immunofluorescence staining of fr...  Read full blog post.

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Bioinformatics

Gene Symbol PKM