PKM2 Antibody [Unconjugated]

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

PKM2 (Pyruvate Kinase isoenzyme M2; also p58, OIP3, THBP1 and CTHBP) is a 58-60 kDa member of the PK family of enzymes. It is widely expressed, being found both intracellularly and in blood, and represents the more common splice variant of the PKM gene. PKM2 generates ATP and pyruvate by catalyzing the transfer of a phosphoryl group from PEP to ADP. Thus, when active, PKM2 promotes energy production and glycolysis. PKM2 exists as a marginally active monomer, with full activity achieved through homotetramerization. Notably, in tumor cells, select oncogenes appear to induce PKM2 homodimerization which limits PKM2 activity. PKM2 is known to be regulated by the binding of T3 and Fru-1,6-bisP. Human PKM2 is 531 amino acids (aa) in length. It contains a catalytic region (aa 43‑527) plus four utilized Ser/Thr and Tyr phosphorylation sites, respectively. PKM1 is another PKM gene splice variant that shows a 45 aa substitution for
aa 389‑433 of PKM2. This variant shows limited expression (striated muscle) and hyperbolic Michaelis-Menten kinetics. There are additional isoform variants of PKM2 that show either a deletion of aa 59‑132, or a 67 aa substitution for aa 1‑82. Over aa 434‑531, human PKM2 shares 95% aa sequence identity with mouse PKM2.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: IHC, Western Blot.

Showing Publications 1 - 3 of 3.

Publications using AF7244	Applications	Species
HM Wise, A Harris, N Kriplani, A Schofield, H Caldwell, MJ Arends, IM Overton, NR Leslie PTEN Protein Phosphatase Activity Is Not Required for Tumour Suppression in the Mouse Prostate Biomolecules, 2022-10-19;12(10):. 2022-10-19 [PMID: 36291720] (IHC, Mouse)	IHC	Mouse
Mei S, Xu Q, Hu Y et al. Integrin B3-PKM2 pathway-mediated aerobic glycolysis contributes to mechanical ventilation-induced pulmonary fibrosis Theranostics 8/15/2022 [PMID: 36168620]
The airway epithelium undergoes metabolic reprogramming in individuals at high risk for lung cancer JCI Insight, 2016-11-17;1(19):e88814. 2016-11-17 [PMID: 27882349] (Western Blot, Human)	Western Blot	Human

Array AF7244

• PKM2 Antibodies
• PKM2 ELISA Kits
• PKM2 Lysates
• PKM2 Proteins
• PKM2 Proteins and Enzyme