| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB120-6405SS | |||
| Azide and BSA Free | NBP2-80848 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-09017-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-65938-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-64952SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | NBP1-28306 | |||
| FITC | NBP1-28305 | |||
![Immunocytochemistry/Immunofluorescence: MHC Class I Antibody (OX18) [NB120-6405] - Major histocompatibility complex (MHC) I and II as well as Transporter associated with antigen presentation II (TAPII) were analyzed, using immunocytochemistry on rat Schwann cells (SCs). Corresponding merges are shown in the bottom rows. Treatment of SCs with IL-17 was performed at concentrations of 0.5 and 50 ng/mL. Graphs to the right show densitometry quantification. SCs showed expression of MHCI > TAPII > MHCII, which increased after IL-17 treatment. MHCI was mainly detected in the cytoplasm and the expression increased in a dose-dependent manner after IL-17 treatment, significant for 0.5 ng/mL and 50 ng/mL (**P TAPII > MHCII, which increased after IL-17 treatment. (A) MHCI was mainly detected in the cytoplasm and the expression increased in a dose-dependent manner after IL-17 treatment, significant for 0.5 ng/mL and 50 ng/mL (**P <=0.01). Image collected and cropped by CiteAb from the following publication (//jneuroinflammation.biomedcentral.com/articles/10.1186/1742-2094-11-63), licensed under a CC-BY licence.](http://images.novusbio.com/fullsize/nb120-6405_mouse-monoclonal-mhc-class-i-antibody-ox18-291220231221972.jpg "Immunocytochemistry/Immunofluorescence: MHC Class I Antibody (OX18) [NB120-6405] - Major histocompatibility complex (MHC) I and II as well as Transporter associated with antigen presentation II (TAPII) were analyzed, using immunocytochemistry on rat Schwann cells (SCs). Corresponding merges are shown in the bottom rows. Treatment of SCs with IL-17 was performed at concentrations of 0.5 and 50 ng/mL. Graphs to the right show densitometry quantification. SCs showed expression of MHCI > TAPII > MHCII, which increased after IL-17 treatment. MHCI was mainly detected in the cytoplasm and the expression increased in a dose-dependent manner after IL-17 treatment, significant for 0.5 ng/mL and 50 ng/mL (**P TAPII > MHCII, which increased after IL-17 treatment. (A) MHCI was mainly detected in the cytoplasm and the expression increased in a dose-dependent manner after IL-17 treatment, significant for 0.5 ng/mL and 50 ng/mL (**P <=0.01). Image collected and cropped by CiteAb from the following publication (//jneuroinflammation.biomedcentral.com/articles/10.1186/1742-2094-11-63), licensed under a CC-BY licence.")
![Western Blot: MHC Class I Antibody (JF10-38) [NBP2-66946] - Analysis of MHC class I on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Positive control: Lane 1: THP-1 cell lysateLane 2: Raji cell lysate](http://images.novusbio.com/fullsize/MHC-Class-I-Antibody-JF10-38-Western-Blot-NBP2-66946-img0004.jpg "Western Blot: MHC Class I Antibody (JF10-38) [NBP2-66946] - Analysis of MHC class I on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Positive control: Lane 1: THP-1 cell lysateLane 2: Raji cell lysate")
![Immunocytochemistry/Immunofluorescence: MHC Class I Antibody (JF10-38) [NBP2-66946] - Staining MHC class I in SH-SY-5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/MHC-Class-I-Antibody-JF10-38-Immunocytochemistry-Immunofluorescence-NBP2-66946-img0002.jpg)
![Immunohistochemistry-Paraffin: MHC Class I Antibody (JF10-38) [NBP2-66946] - Analysis of paraffin-embedded human spleen tissue using anti-MHC class I antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/MHC-Class-I-Antibody-JF10-38-Immunohistochemistry-Paraffin-NBP2-66946-img0003.jpg)
![Flow Cytometry: MHC Class I Antibody (JF10-38) [NBP2-66946] - Analysis of THP-1 cells with MHC class I antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody](http://images.novusbio.com/fullsize/MHC-Class-I-Antibody-JF10-38-Flow-Cytometry-NBP2-66946-img0001.jpg)
![Western Blot: MHC Class I Antibody (R1-21.2) - Chimeric [NBP3-09017] - Mouse thymus tissue lysate (35ug protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with NBP3-09017 at 1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/MHC-Class-I-Antibody-R1-21-2-Chimeric-Western-Blot-NBP3-09017-img0003.jpg "Western Blot: MHC Class I Antibody (R1-21.2) - Chimeric [NBP3-09017] - Mouse thymus tissue lysate (35ug protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with NBP3-09017 at 1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: MHC Class I Antibody (R1-21.2) - Chimeric [NBP3-09017] - Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized and stained with NBP3-09017 at 10 ug/ml followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing membrane staining in a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-09017, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (NBP2-52638) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/MHC-Class-I-Antibody-R1-21-2-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-09017-img0002.jpg)
![Immunohistochemistry: MHC Class I Antibody (MEM-E/06) [NB500-307] - Staining of human spleen (paraffin sections) with anti-HLA-E (clone MEM-E/06).](http://images.novusbio.com/fullsize/MHC-Class-I-Antibody-MEM-E-06-Immunohistochemistry-NB500-307-img0001.jpg "Immunohistochemistry: MHC Class I Antibody (MEM-E/06) [NB500-307] - Staining of human spleen (paraffin sections) with anti-HLA-E (clone MEM-E/06).")
![Flow Cytometry: MHC Class I Antibody (MEM-E/06) [NB500-307] - Analysis using PE conjugate of NB500-307. A cell surface stain was performed on THP-1 cells with MHC Class I antibody (MEM-E/06) NB500-307 (blue) and a matched isotype control NBP2-27287 (orange). Cells were incubated in an antibody dilution of 1:100 for 20 minutes at room temperature. Both antibodies were conjugated to PE.](http://images.novusbio.com/fullsize/MHC-Class-I-Antibody-MEM-E-06-Flow-Cytometry-NB500-307-img0002.jpg)
![Flow Cytometry: MHC Class I Antibody (H58A) [NBP2-61002] - Obtained with canine leukocytes.](http://images.novusbio.com/fullsize/MHC-Class-I-Antibody-H58A-Flow-Cytometry-NBP2-61002-img0002.jpg "Flow Cytometry: MHC Class I Antibody (H58A) [NBP2-61002] - Obtained with canine leukocytes.")
![Flow Cytometry: MHC Class I Antibody (H58A) [NBP2-61002] - Obtained with feline leukocytes.](http://images.novusbio.com/fullsize/MHC-Class-I-Antibody-H58A-Flow-Cytometry-NBP2-61002-img0001.jpg)
![Western Blot: MHC Class I Antibody (3I1C1) [NBP3-16696] - Western blot analysis of extracts of various cell lines, using MHC Class I Rabbit mAb (NBP3-16696) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/MHC-Class-I-Antibody-3I1C1-Western-Blot-NBP3-16696-img0002.jpg "Western Blot: MHC Class I Antibody (3I1C1) [NBP3-16696] - Western blot analysis of extracts of various cell lines, using MHC Class I Rabbit mAb (NBP3-16696) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunocytochemistry/Immunofluorescence: MHC Class I Antibody (3I1C1) [NBP3-16696] - Immunofluorescence analysis of THP-1 cells using MHC Class I antibody (NBP3-16696) at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/MHC-Class-I-Antibody-3I1C1-Immunocytochemistry-Immunofluorescence-NBP3-16696-img0001.jpg)
![Flow Cytometry: MHC Class I Antibody (F21-2) [NBP1-28304] - Chicken peripheral blood lymphocytes were stained with Mouse Anti-Chicken MHC Class I-UNLB followed by Goat Anti-Mouse IgG1, Human ads-FITC.](http://images.novusbio.com/fullsize/MHC-Class-I-Antibody-F21-2-Flow-Cytometry-NBP1-28304-img0003.jpg "Flow Cytometry: MHC Class I Antibody (F21-2) [NBP1-28304] - Chicken peripheral blood lymphocytes were stained with Mouse Anti-Chicken MHC Class I-UNLB followed by Goat Anti-Mouse IgG1, Human ads-FITC.")
![Flow Cytometry: MHC Class I Antibody (F21-2) [NBP1-28304] - Analysis using the Biotin conjugate of NBP1-28304. Multiple staining of peripheral blood mononuclear cells.](http://images.novusbio.com/fullsize/MHC-Class-I-Antibody-F21-2-Flow-Cytometry-NBP1-28304-img0002.jpg)
![Flow Cytometry: MHC Class I Antibody (MEM-E/08) [NB500-305] - Flow cytometry analysis (surface staining) of HLA-E-transfectants with anti-HLA-E (MEM-E/08) purified, GAM-APC](http://images.novusbio.com/fullsize/MHC-Class-I-Antibody-MEM-E-08-Flow-Cytometry-NB500-305-img0001.jpg "Flow Cytometry: MHC Class I Antibody (MEM-E/08) [NB500-305] - Flow cytometry analysis (surface staining) of HLA-E-transfectants with anti-HLA-E (MEM-E/08) purified, GAM-APC")