| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide Free | NBP2-27149 | |||
| BSA Free | NB100-56566SS | |||
| Azide Free | NBP2-27149SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-80652-0.1mg | |||
| BSA Free | NB100-77417SS | |||
| Azide and BSA Free | NBP2-80652 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-77477SS | |||
| Azide and BSA Free | NBP2-80624 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-25255SS | |||
| Azide and BSA Free | NBP2-80588 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-43359-0.05mg | |||
| BSA Free | NBP1-43359-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-80811 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-19015SS | |||
| Azide and BSA Free | NBP2-80807 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide Free | NBP2-27405 | |||
| BSA Free | NBP2-27404SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-03948SS | |||
| Azide and BSA Free | NBP2-80881 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| PE | NBP2-27252 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide Free | NBP2-25229 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54392-100ug | |||
![Dual RNAscope ISH-IHC: TLR4 Antibody (76B357.1) [NB100-56566] - FFPE tissue sections of human tonsil were probed for TLR4 mRNA (ACD RNAScope Probe, ACD catalog # 311281; Fast Red chromogen, ACD catalog # 322750). Adjacent tissue section was processed for immunohistochemistry using Mouse Monoclonal (Novus Biologicals catalog # NB100-56566) at 5ug/mL with 1 hour incubation at room temperature followed by incubation with anti-mouse IgG VisUCyte HRP Polymer Antibody (Catalog # VC001) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes.](http://images.novusbio.com/fullsize/TLR4-Antibody-76B357-1-Dual-RNAscope-ISH-IHC-NB100-56566-img0036.jpg "<b>Orthogonal Strategies Validation. </b>Dual RNAscope ISH-IHC: TLR4 Antibody (76B357.1) [NB100-56566] - FFPE tissue sections of human tonsil were probed for TLR4 mRNA (ACD RNAScope Probe, ACD catalog # 311281; Fast Red chromogen, ACD catalog # 322750). Adjacent tissue section was processed for immunohistochemistry using Mouse Monoclonal (Novus Biologicals catalog # NB100-56566) at 5ug/mL with 1 hour incubation at room temperature followed by incubation with anti-mouse IgG VisUCyte HRP Polymer Antibody (Catalog # VC001) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes.")
![<b>Orthogonal Strategies Validation. </b>Flow Cytometry: TLR4 Antibody (76B357.1) [NB100-56566] - Analysis using the Alexa Fluor (R) 647 conjugate of NBP2-27149. TLR4 expression on monocytes from human peripheral blood. PBMC were stained in a 2 color flow test, with CD14 PE version of this antibody and 1 ug of either isotype control (left) or TLR4-Alexa Fluor 647 (right). PPI negative, CD14+ cells were gated for analysis.](http://images.novusbio.com/fullsize/TLR4-Antibody-76B357-1-Flow-Cytometry-NB100-56566-img0024.jpg)
![Immunohistochemistry: TLR4 Antibody (76B357.1) [NB100-56566] - Pericryptal Myofibroblasts are Responsible for Increased TLR4 Expression in a Subset of CRCs. Double-stained immunofluorescence for TLR4 (green) and vimentin (red) in normal (I), adenoma (II), and colon adenocarcinoma (III) (10x). In the stromal compartment of CRCs, immunofluorescent staining for TLR4 localized to the pericryptal myofibroblasts in a subset of samples. Image collected and cropped by CiteAb from the following publication (//www.jeccr.com/content/33/1/45), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TLR4-Antibody-76B357-1-Immunohistochemistry-NB100-56566-img0040.jpg)
![Western Blot: TLR4 Antibody (76B357.1) [NB100-56566] - Analysis using 2 ug/mL on (A) human intestine and 6 ug/mL on (B) mouse intestine and (C) rat intestine lysate.](http://images.novusbio.com/fullsize/TLR4-Antibody-76B357-1-Western-Blot-NB100-56566-img0018.jpg)
![Immunohistochemistry: TLR4 Antibody (76B357.1) [NB100-56566] - Pericryptal Myofibroblasts are Responsible for Increased TLR4 Expression in a Subset of CRCs. IHC staining of colon adenocarcinoma for TLR4, vimentin, and alpha-SMA (40x). Staining co-localizes to the pericryptal space, confirming the signal arises from pericryptal myofibroblasts. Image collected and cropped by CiteAb from the following publication (//www.jeccr.com/content/33/1/45), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TLR4-Antibody-76B357-1-Immunohistochemistry-NB100-56566-img0041.jpg)
![Flow (Intracellular): TLR4 Antibody (76B357.1) [NB100-56566] - Analysis using PE conjugate of NBP2-27149. An intracellular stain was performed on Jurkat cells with TLR4 antibody (76B357.1) NBP2-27149PE (blue) and an isotype control MAB004 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to phycoerythrin.](http://images.novusbio.com/fullsize/TLR4-Antibody-76B357-1-Flow-Intracellular-NB100-56566-img0025.jpg)
![Immunocytochemistry/Immunofluorescence: TLR4 Antibody (76B357.1) [NB100-56566] - RH-30 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-TLR4 Antibody (76B357.1) NB100-56566 at 1 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/TLR4-Antibody-76B357-1-Immunocytochemistry-Immunofluorescence-NB100-56566-img0042.jpg)
![Immunohistochemistry: TLR4 Antibody (76B357.1) [NB100-56566] - Immunofluorescent staining of TMAs. Representative tissue cores from normal (I), adenomatous polyps (II), and CRC (III and IV) are shown. Image collected and cropped by CiteAb from the following publication (//www.jeccr.com/content/33/1/45), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TLR4-Antibody-76B357-1-Immunohistochemistry-NB100-56566-img0037.jpg)
![Immunohistochemistry-Paraffin: TLR4 Antibody (76B357.1) [NB100-56566] - Analysis of rat salivary gland tissue section at 1:100 dilution. The antibody generated a membrane-cytoplasmic staining in the tissue with stronger signal in ductal epithelial cells.](http://images.novusbio.com/fullsize/TLR4-Antibody-76B357-1-Immunohistochemistry-Paraffin-NB100-56566-img0015.jpg)
![Immunohistochemistry-Paraffin: TLR4 Antibody (76B357.1) [NB100-56566] - Tissue section of normal human skin stained with antibody at 5 ug/mL. Membrane-cytoplasmic immunopositivity of TLR4 was primarily observed in the pigmented basel cells and the adjacent keratinocytes in the epidermal layer.](http://images.novusbio.com/fullsize/TLR4-Antibody-76B357-1-Immunohistochemistry-Paraffin-NB100-56566-img0013.jpg)
![Flow Cytometry: TLR4 Antibody (76B357.1) [NB100-56566] - An intracellular stain was performed on RH30 cells with TLR4 Antibody (76B357.1) NB100-56566 (blue) and a matched mouse IgG2b Kappa isotype control (orange) MAB004. Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (35503, Thermo Fisher).](http://images.novusbio.com/fullsize/TLR4-Antibody-76B357-1-Flow-Cytometry-NB100-56566-img0045.jpg)
![Immunohistochemistry-Paraffin: TLR4 Antibody (76B357.1) [NB100-56566] - Human skin stained with at 5 ug/mL, peroxidase-conjugate and DAB chromogen. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.](http://images.novusbio.com/fullsize/TLR4-Antibody-76B357-1-Immunohistochemistry-Paraffin-NB100-56566-img0007.jpg)
![Immunohistochemistry-Paraffin: TLR4 Antibody (76B357.1) [NB100-56566] - Analysis of TLR4 in FFPE human colon tissue using an isotype control (top) and NB100-56566 (bottom) at 5 ug/mL.](http://images.novusbio.com/fullsize/TLR4-Antibody-76B357-1-Immunohistochemistry-Paraffin-NB100-56566-img0016.jpg)
![Immunohistochemistry-Paraffin: TLR4 Antibody (76B357.1) [NB100-56566] - Human testis tissue stained with antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/TLR4-Antibody-76B357-1-Immunohistochemistry-Paraffin-NB100-56566-img0017.jpg)
![Immunohistochemistry-Frozen: TLR4 Antibody (76B357.1) [NB100-56566] - This image is TLR4(green) and nucleus(blue) at area postrema of the adult male mouse brain, x20 magnification. Primary antibody diluted 1:500. IHC-Fr image submitted by a verified customer review.](http://images.novusbio.com/fullsize/TLR4-Antibody-76B357-1-Immunohistochemistry-Frozen-NB100-56566-img0032.jpg)
![Flow Cytometry: TLR4 Antibody (76B357.1) [NB100-56566] - Analysis of formaldehyde fixed THP-1 cells (human monocytic leukemia cells) using 2 ug/10^6 cells TLR4 antibody (clone 76B357.1) with detection employing a donkey anti-mouse IgG (H+L) cross adsorbed secondary antibody, (DyLight 488 conjugated). Isotype control samples incubated with mouse IgG2b isotype control antibody were processed in parallal under the same assay conditions.](http://images.novusbio.com/fullsize/TLR4-Antibody-76B357-1-Flow-Cytometry-NB100-56566-img0031.jpg)
![Flow Cytometry: TLR4 Antibody (76B357.1) [NB100-56566] - An intracellular stain was performed on Jurkat cells with TLR4 Antibody (76B357.1) NB100-56566 (blue) and a matched mouse IgG2b Kappa isotype control (orange) MAB004. Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (35503, Thermo Fisher).](http://images.novusbio.com/fullsize/TLR4-Antibody-76B357-1-Flow-Cytometry-NB100-56566-img0043.jpg)
![Flow Cytometry: TLR4 Antibody (76B357.1) [NB100-56566] - An intracellular stain was performed on Raw264.7 cells with TLR4 Antibody (76B357.1) NB100-56566 (blue) and a matched mouse IgG2b Kappa isotype control (orange) MAB004. Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (35503, Thermo Fisher).](http://images.novusbio.com/fullsize/TLR4-Antibody-76B357-1-Flow-Cytometry-NB100-56566-img0044.jpg)
![Flow Cytometry: CD45 Antibody (30-F11) [NB100-77417] - Analysis of CD45 (30F11) in freshly isolated mouse (Balb C) splenocytes (1x10^6) were stained with [NB100-77417] at 1:1000 dilution and detected with FITC conjugated donkey anti-rat IgG secondary (orange). Shown with rat IgG isotype control (blue).](http://images.novusbio.com/fullsize/CD45-Antibody-30-F11-Flow-Cytometry-NB100-77417-img0004.jpg "Flow Cytometry: CD45 Antibody (30-F11) [NB100-77417] - Analysis of CD45 (30F11) in freshly isolated mouse (Balb C) splenocytes (1x10^6) were stained with [NB100-77417] at 1:1000 dilution and detected with FITC conjugated donkey anti-rat IgG secondary (orange). Shown with rat IgG isotype control (blue).")
![Immunohistochemistry-Paraffin: CD45 Antibody (30-F11) [NB100-77417] - Analysis of FFPE section of mouse spleen using rat monoclonal CD45 antibody (30-F11) [NB100-77417] at 1:50 dilution. The antibody generated an expected membrane staining in the red and white pulp spleenocytes of the tested spleen section.](http://images.novusbio.com/fullsize/CD45-Antibody-30-F11-Immunohistochemistry-Paraffin-NB100-77417-img0006.jpg)
![Immunohistochemistry-Paraffin: CD45 Antibody (30-F11) [NB100-77417] - Mouse glomeruli stained with CD45 antibody. 4% formaldehyde fixed paraffin sections. EDTA was used for antigen retrieval. IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/CD45-Antibody-30-F11-Immunohistochemistry-Paraffin-NB100-77417-img0013.jpg)
![Immunohistochemistry: CD45 Antibody (30-F11) [NB100-77417] - Analysis of a FFPE embedded tissue section of mouse liver using 1:100 dilution of CD45 antibody (30-F11) [NB100-77417]. The signal was developed using AEC reagent based method with hematoxylin counterstaining.](http://images.novusbio.com/fullsize/CD45-Antibody-30-F11-Immunohistochemistry-NB100-77417-img0003.jpg)
![Immunohistochemistry-Frozen: CD45 Antibody (30-F11) [NB100-77417] - Analysis of a mouse spleen cryosection using CD45 antibody (30-F11) [NB100-77417] at 1:50 dilution. The signal was detected using goat anti-rat Alexa fluor 488 secondary with DAPI counterstaining. IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/CD45-Antibody-30-F11-Immunohistochemistry-Frozen-NB100-77417-img0008.jpg)
![Immunohistochemistry-Paraffin: CD45 Antibody (30-F11) [NB100-77417] - Staining of a FFPE mouse liver tissue section using 1:100 dilution of CD45 antibody (30-F11) [NB100-77417]. Signal was detected using AF488 conjugated donkey anti-rat secondary antibody at 1:500 dilution. IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/CD45-Antibody-30-F11-Immunohistochemistry-Paraffin-NB100-77417-img0002.jpg)
![Immunohistochemistry: CD45 Antibody (30-F11) [NB100-77417] - Severe apoptosis and pathology in the orally infected heart. Experimental design is as outlined in Fig. 1B. Heart sections from moribund mice were examined with H&E, and immunohistochemistry staining for pan-leukocyte infiltration using an anti-CD45 antibody. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-019-47455-3), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CD45-Antibody-30-F11-Immunohistochemistry-NB100-77417-img0009.jpg)
![Immunohistochemistry: CD45 Antibody (30-F11) [NB100-77417] - Severe apoptosis and pathology in the orally infected heart. Experimental design is as outlined in Fig. 1B. Heart sections from moribund mice were examined with H&E, and immunohistochemistry staining for pan-leukocyte infiltration using an anti-CD45 antibody. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-019-47455-3), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CD45-Antibody-30-F11-Immunohistochemistry-NB100-77417-img0012.jpg)
![Immunohistochemistry: CD45 Antibody (30-F11) [NB100-77417] - VP1-positive infiltrating M2 macrophage and VP1-positive pneumocytes were detectable in the orally infected lung. Adjacent lung sections were examined with H&E and IHC staining for a pan-leukocyte marker CD45. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-019-47455-3), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CD45-Antibody-30-F11-Immunohistochemistry-NB100-77417-img0011.jpg)
![Flow Cytometry: CD117/c-kit Antibody (2B8) [NB100-77477] - Detection of CD117/c-kit in Mouse Bone Marrow Cells by Flow Cytometry. Mouse bone marrow cells were stained with PE-conjugated Rat Anti-Mouse B220/CD45R (Catalog # FAB1217P) and Rat Anti-Mouse CD117/c-kit Monoclonal Antibody (Catalog # NB100-77477, top panel) or isotype control antibody (Catalog # MAB0061, bottom panel), followed by APC-conjugated Goat Anti-Rat IgG (Catalog # F0113).](http://images.novusbio.com/fullsize/CD117-c-kit-Antibody-2B8-Flow-Cytometry-NB100-77477-img0003.jpg "Flow Cytometry: CD117/c-kit Antibody (2B8) [NB100-77477] - Detection of CD117/c-kit in Mouse Bone Marrow Cells by Flow Cytometry. Mouse bone marrow cells were stained with PE-conjugated Rat Anti-Mouse B220/CD45R (Catalog # FAB1217P) and Rat Anti-Mouse CD117/c-kit Monoclonal Antibody (Catalog # NB100-77477, top panel) or isotype control antibody (Catalog # MAB0061, bottom panel), followed by APC-conjugated Goat Anti-Rat IgG (Catalog # F0113).")
![Flow Cytometry: CD117/c-kit Antibody (2B8) [NB100-77477] - Identification of CD117/c-kit expressing bone marrow cells.Double labeling of mouse bone marrow cells with anti-CD45 antibody and rabbit anti-CD117/c-kit antibody 2B8. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0071798) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CD117-c-kit-Antibody-2B8-Flow-Cytometry-NB100-77477-img0004.jpg)
![Flow Cytometry: CD117/c-kit Antibody (2B8) [NB100-77477] - C57BL/6 bone marrow cells stained with 2B8 PE and CD11b FITC.](http://images.novusbio.com/fullsize/CD117-c-kit-Antibody-2B8-Flow-Cytometry-NB100-77477-img0002.jpg)
![Western Blot: B7-1/CD80 Antibody (62N3G8) [NBP2-25255] - Analysis in human Raji lymphoma cells.](http://images.novusbio.com/fullsize/B7-1-CD80-Antibody-62N3G8-Western-Blot-NBP2-25255-img0010.jpg "Western Blot: B7-1/CD80 Antibody (62N3G8) [NBP2-25255] - Analysis in human Raji lymphoma cells.")
![Immunohistochemistry-Paraffin: B7-1/CD80 Antibody (62N3G8) [NBP2-25255] - Human thymus stained with CD80 antibody at 5 ug/ml using peroxidase-conjugate and DAB chromogen. Note membrane and cytoplasmic staining.](http://images.novusbio.com/fullsize/B7-1-CD80-Antibody-62N3G8-Immunohistochemistry-Paraffin-NBP2-25255-img0002.jpg)
![Flow Cytometry: B7-1/CD80 Antibody (62N3G8) [NBP2-25255] - Raji lymphoma cells. Image from verified customer review.](http://images.novusbio.com/fullsize/B7-1-CD80-Antibody-62N3G8-Flow-Cytometry-NBP2-25255-img0009.jpg)
![Western Blot: B7-1/CD80 Antibody (62N3G8) [NBP2-25255] - Analysis of CD80 in A) a partial recombinant protein and B) Daudi lysate using this antibody at 0.1 and 1 g/ml, respectively. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/B7-1-CD80-Antibody-62N3G8-Western-Blot-NBP2-25255-img0003.jpg)
![Flow Cytometry: B7-1/CD80 Antibody (62N3G8) [NBP2-25255] - Analysis using the Alexa Fluor (R) 700 conjugate of NBP2-25255. Staining of CD80 in human PBMC (monocyte depleted) using anti-CD80 antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/B7-1-CD80-Antibody-62N3G8-Flow-Cytometry-NBP2-25255-img0008.jpg)
![Immunohistochemistry Whole-Mount: CD117/c-kit Antibody (ACK2) [NBP1-43359] - Fluorescent images from representative ileum slices show putative neuronal cells and interstitial cells of Cajal. Immunoreactive c-Kit was localized by using the ACK2 antibody in live tissue after 48 h ex vivo. Scale bars are 25 um. Image collected and cropped by CiteAb from the following publication (//www.physiology.org/doi/10.1152/ajpgi.00299.2015) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CD117-c-kit-Antibody-ACK2-Immunohistochemistry-Whole-Mount-NBP1-43359-img0003.jpg "Immunohistochemistry Whole-Mount: CD117/c-kit Antibody (ACK2) [NBP1-43359] - Fluorescent images from representative ileum slices show putative neuronal cells and interstitial cells of Cajal. Immunoreactive c-Kit was localized by using the ACK2 antibody in live tissue after 48 h ex vivo. Scale bars are 25 um. Image collected and cropped by CiteAb from the following publication (//www.physiology.org/doi/10.1152/ajpgi.00299.2015) licensed under a CC-BY license.")
![Flow Cytometry: CD117/c-kit Antibody (ACK2) [NBP1-43359] - Analysis of Allophycocyanin conjugate of NBP1-43359. Staining of C57BL/6 bone marrow cells with Anti-Human/Mouse CD45R (B220) FITC and 0.03 ug of Rat IgG2b kappa Isotype Control APC (left) or 0.03 ug of Anti-Mouse CD117 (c-Kit) APC (right). Total viable c](http://images.novusbio.com/fullsize/CD117-c-kit-Antibody-ACK2-Flow-Cytometry-NBP1-43359-img0001.jpg)
![Flow Cytometry: CD117/c-kit Antibody (ACK2) [NBP1-43359] - Analysis of PE/Cy5 conjugate of NBP1-43359. Staining of C57Bl/6 bone marrow cells with Anti-Human/Mouse CD45R (B220) FITC and 0.03 ug of Rat IgG2b Isotype Control PE-Cy5 (left) or 0.03 ug of Anti-Mouse CD117 (c-Kit) PE-Cy5 (right). Total viable cells were](http://images.novusbio.com/fullsize/CD117-c-kit-Antibody-ACK2-Flow-Cytometry-NBP1-43359-img0002.jpg)
![Western Blot: IL-3 R alpha/CD123 Antibody (18N6B12) [NBP2-27225] - Analysis of CD123 in the A) presence and B) absence of equal amounts antibody and immunizing peptide in human SKNSH cell lysate using CD123 antibody at 10 ug/ml.](http://images.novusbio.com/fullsize/IL-3-R-alpha-CD123-Antibody-18N6B12-Western-Blot-NBP2-27225-img0001.jpg "Western Blot: IL-3 R alpha/CD123 Antibody (18N6B12) [NBP2-27225] - Analysis of CD123 in the A) presence and B) absence of equal amounts antibody and immunizing peptide in human SKNSH cell lysate using CD123 antibody at 10 ug/ml.")
![Flow Cytometry: IL-3 R alpha/CD123 Antibody (18N6B12) [NBP2-27225] - Analysis using the Alexa Fluor (R) 700 conjugate of NBP2-27225. Staining of CD123 in human buffy coat using anti-CD123 antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/IL-3-R-alpha-CD123-Antibody-18N6B12-Flow-Cytometry-NBP2-27225-img0006.jpg)
![Flow Cytometry: IL-3 R alpha/CD123 Antibody (18N6B12) [NBP2-27225] - Cell surface analysis using CD123 antibody. BDCM cells were probed using 1 ug of CD123 antibody (red) or 1 ug of isotype control (green). This IL-3 R alpha/CD123 antibody and a mouse IgG-PE secondary antibody were used for this test.](http://images.novusbio.com/fullsize/IL-3-R-alpha-CD123-Antibody-18N6B12-Flow-Cytometry-NBP2-27225-img0002.jpg)
![Flow Cytometry: IL-3 R alpha/CD123 Antibody (18N6B12) [NBP2-27225] - Analysis using the Alexa Fluor (R) 647 conjugate of NBP2-27225. Staining of CD123 in human buffy coat using anti-CD123 antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/IL-3-R-alpha-CD123-Antibody-18N6B12-Flow-Cytometry-NBP2-27225-img0005.jpg)
![Immunohistochemistry-Paraffin: IL-27 R alpha/WSX-1/TCCR Antibody (34N4G11) [NBP2-19015] - IHC-P detection of IL27RA protein in a section of human renal/kidney cancer tissue using IL27RA antibody (34N4G11) at a concentration of 5ug/ml. The representative image shows membrane-cytoplasmic positivity for IL27RA protein in the cancer cells.](http://images.novusbio.com/fullsize/IL-27-R-alpha-WSX-1-TCCR-Antibody-34N4G11-Immunohistochemistry-Paraffin-NBP2-19015-img0010.jpg "Immunohistochemistry-Paraffin: IL-27 R alpha/WSX-1/TCCR Antibody (34N4G11) [NBP2-19015] - IHC-P detection of IL27RA protein in a section of human renal/kidney cancer tissue using IL27RA antibody (34N4G11) at a concentration of 5ug/ml. The representative image shows membrane-cytoplasmic positivity for IL27RA protein in the cancer cells.")
![Flow (Cell Surface): IL-27 R alpha/WSX-1/TCCR Antibody (34N4G11) [NBP2-19015] - Analysis using the Alexa Fluor (R) 647 conjugate of NBP2-19015. Surface staining of IL27R on freshly isolated (PPI negative) human PBMCs using IL-27R antibody and CD3 antibody at 10 ul/10^6 cells. Flow kit, mouse IgG2b isotype control antibody, and RBC lysis buffer were used for this test.](http://images.novusbio.com/fullsize/IL-27-R-alpha-WSX-1-TCCR-Antibody-34N4G11-Flow-Cell-Surface-NBP2-19015-img0012.jpg)
![Immunohistochemistry-Paraffin: IL-27 R alpha/WSX-1/TCCR Antibody (34N4G11) [NBP2-19015] - Adenocarcinoma of the stomach probed with IL-27R antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/IL-27-R-alpha-WSX-1-TCCR-Antibody-34N4G11-Immunohistochemistry-Paraffin-NBP2-19015-img0004.jpg)
![Immunohistochemistry-Paraffin: IL-27 R alpha/WSX-1/TCCR Antibody (34N4G11) [NBP2-19015] - FFPE human prostate probed with IL-27R antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/IL-27-R-alpha-WSX-1-TCCR-Antibody-34N4G11-Immunohistochemistry-Paraffin-NBP2-19015-img0005.jpg)
![Immunohistochemistry-Paraffin: IL-27 R alpha/WSX-1/TCCR Antibody (34N4G11) [NBP2-19015] - IHC-P detection of IL27RA protein in a section of normal human colon tissue using IL27RA antibody (34N4G11) at a concentration of 5ug/ml. Intense membrane with cytoplasmic staining was observed in the cells of crypts and the absorptive columnar epithelium.](http://images.novusbio.com/fullsize/IL-27-R-alpha-WSX-1-TCCR-Antibody-34N4G11-Immunohistochemistry-Paraffin-NBP2-19015-img0006.jpg)
![Immunohistochemistry-Paraffin: IL-27 R alpha/WSX-1/TCCR Antibody (34N4G11) [NBP2-19015] - IHC-P detection of IL27RA protein in a section of human colo-rectal cancer tissue using IL27RA antibody (34N4G11) at a concentration of 5ug/ml. The cancer cells showed specific positivity for IL27RA and the staining intensity was more towards the cellular membranes. The tumor stroma was largely negative for staining.](http://images.novusbio.com/fullsize/IL-27-R-alpha-WSX-1-TCCR-Antibody-34N4G11-Immunohistochemistry-Paraffin-NBP2-19015-img0007.jpg)
![Immunohistochemistry-Paraffin: IL-27 R alpha/WSX-1/TCCR Antibody (34N4G11) [NBP2-19015] - IHC-P detection of IL27RA protein in a section of human stomach cancer tissue using IL27RA antibody (34N4G11) at a concentration of 5ug/ml. The cancer cells showed intense membrane-cytoplasmic positivity for IL27RA protein, whereas, the glands in adjacent area and the associated stroma developed distinctly weak staining.](http://images.novusbio.com/fullsize/IL-27-R-alpha-WSX-1-TCCR-Antibody-34N4G11-Immunohistochemistry-Paraffin-NBP2-19015-img0009.jpg)
![Flow Cytometry: IL-27 R alpha/WSX-1/TCCR Antibody (34N4G11) [NBP2-19015] - Surface staining on human peripheral blood monocytes using IL-27R antibody at 1 ug/10^6 cells (red represents specific antibody and green represents isotype control). APC-conjugated goat anti-mouse Ig F(ab')2 secondary antibody , CS flow kit and an FcR block were used for this test.](http://images.novusbio.com/fullsize/IL-27-R-alpha-WSX-1-TCCR-Antibody-34N4G11-Flow-Cytometry-NBP2-19015-img0001.jpg)
![Western Blot: TLR3 Antibody (27N3D4) [NBP2-27404] - Analysis of TLR3 using TLR3 monoclonal antibody. Human intestine (A), human spleen (B), and mouse RAW lysate (C) probed with TLR3 antibody at 2 ug/ml. I goat anti-rat Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/TLR3-Antibody-27N3D4-Western-Blot-NBP2-27404-img0001.jpg "Western Blot: TLR3 Antibody (27N3D4) [NBP2-27404] - Analysis of TLR3 using TLR3 monoclonal antibody. Human intestine (A), human spleen (B), and mouse RAW lysate (C) probed with TLR3 antibody at 2 ug/ml. I goat anti-rat Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Flow Cytometry: TLR3 Antibody (27N3D4) [NBP2-27404] - Analysis of TLR3 using TLR3 monoclonal antibody. Mouse splenocytes were probed with TLR3 antibody (red) and isotype control antibody (green) at 1 ug/10^6 cells.](http://images.novusbio.com/fullsize/TLR3-Antibody-27N3D4-Flow-Cytometry-NBP2-27404-img0009.jpg)
![Flow Cytometry: TLR3 Antibody (27N3D4) [NBP2-27404] - Analysis using Azide Free version of NBP2-27404. TLR3 using TLR3 monoclonal antibody. Mouse RAW cells and splenocytes were probed with TLR3 antibody (red) and isotype control antibody (green) at 1 ug/10^6 cells.](http://images.novusbio.com/fullsize/TLR3-Antibody-27N3D4-Flow-Cytometry-NBP2-27404-img0006.jpg)
![Flow Cytometry: TLR3 Antibody (27N3D4) [NBP2-27404] - Analysis using Azide Free version of NBP2-27404. TLR3 using TLR3 monoclonal antibody. Mouse RAW cells were probed with TLR3 antibody (red) and isotype control antibody (green) at 0.1 ug/10^6 cells.](http://images.novusbio.com/fullsize/TLR3-Antibody-27N3D4-Flow-Cytometry-NBP2-27404-img0007.jpg)
![Flow Cytometry: TLR3 Antibody (27N3D4) [NBP2-27404] - Analysis of TLR3 using TLR3 monoclonal antibody. Mouse RAW cells were probed with TLR3 antibody (red) and isotype control antibody (green) at 1 ug/10^6 cells.](http://images.novusbio.com/fullsize/TLR3-Antibody-27N3D4-Flow-Cytometry-NBP2-27404-img0008.jpg)
![Simple Western: NLRP3/NALP3 Antibody (25N10E9) [NBP2-03948] - Simple Western lane view shows a specific band for NALP3 in 0.5 mg/ml of RAW lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/NLRP3-NALP3-Antibody-25N10E9-Simple-Western-NBP2-03948-img0004.jpg "Simple Western: NLRP3/NALP3 Antibody (25N10E9) [NBP2-03948] - Simple Western lane view shows a specific band for NALP3 in 0.5 mg/ml of RAW lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.")
![Western Blot: NLRP3/NALP3 Antibody (25N10E9) [NBP2-03948] - Analysis of Raw 264 cell lysate using NLRP3/NALP3 antibody (NBP2-03948) at 2 ug/ml.](http://images.novusbio.com/fullsize/NLRP3-NALP3-Antibody-25N10E9-Western-Blot-NBP2-03948-img0008.jpg)
![Western Blot: NLRP3/NALP3 Antibody (25N10E9) [NBP2-03948] - Analysis using the NALP3 monoclonal antibody in mouse NALP3 transfected cell lysate (A), human NALP3 transfected cell lysate (B), mouse RAW lysate (C) and the recombinant mouse NLRP3 NOD domain protein immunogen (D) probed with 2 ug/ml antibody. Goat anti-mouse IgG HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/NLRP3-NALP3-Antibody-25N10E9-Western-Blot-NBP2-03948-img0007.jpg)
![Western Blot: IL-17/IL-17A Antibody (4H1524) [NBP2-27338] - Analysis of IL-17A in 1) human, 2) mouse and 3) rat full-length recombinant IL-17A protein using NBP2-27338 at 1 ug/ml.](http://images.novusbio.com/fullsize/IL-17-IL-17A-Antibody-4H1524-Western-Blot-NBP2-27338-img0004.jpg "Western Blot: IL-17/IL-17A Antibody (4H1524) [NBP2-27338] - Analysis of IL-17A in 1) human, 2) mouse and 3) rat full-length recombinant IL-17A protein using NBP2-27338 at 1 ug/ml.")
![<b>Biological Strategies Validation. </b>Flow Cytometry: IL-17/IL-17A Antibody (4H1524) [NBP2-27338] - Analysis using the PE conjugate of NBP2-25229. IL-17A positive cells in PMA/IM stimulated fresh PBMC: PBMC stimulated for 6h with PMA (25ng/ml) & IM (250ng/ml), in the presence of BFA. Cells were surface stained with CD4 APC, fixed & permeabilised for intracellular staining with PE conjugated](http://images.novusbio.com/fullsize/IL-17-IL-17A-Antibody-4H1524-Flow-Cytometry-NBP2-27338-img0007.jpg)
![Block/Neutralize: IL-17/IL-17A Antibody (4H1524) [NBP2-27338] - Analysis using Azide Free version of NBP2-27338. Neutralization by Human IL-17A Antibody. Secretion of IL-6 by 3T3 cells stimulated with recombinant human IL-17A (20ng/ml) is neutralized by increasing concentrations of IL-17A monoclonal antibody. Antibody](http://images.novusbio.com/fullsize/IL-17-IL-17A-Antibody-4H1524-Block-Neutralize-NBP2-27338-img0006.jpg)
![Flow Cytometry: CD20 Antibody (2H7) [NBP2-62206] - Surface staining of human peripheral blood with anti-CD20 (2H7) biotin, streptavidin.](http://images.novusbio.com/fullsize/CD20-Antibody-2H7-Flow-Cytometry-NBP2-62206-img0012.jpg "Flow Cytometry: CD20 Antibody (2H7) [NBP2-62206] - Surface staining of human peripheral blood with anti-CD20 (2H7) biotin, streptavidin.")
![Flow Cytometry: CD20 Antibody (2H7) [NBP2-62206] - Surface staining of human peripheral blood with anti-CD20 (2H7) PE.](http://images.novusbio.com/fullsize/CD20-Antibody-2H7-Flow-Cytometry-NBP2-62206-img0001.jpg)
![Flow Cytometry: CD20 Antibody (2H7) [NBP2-62206] - Surface staining of human peripheral blood with anti-CD20 (2H7) FITC.](http://images.novusbio.com/fullsize/CD20-Antibody-2H7-Flow-Cytometry-NBP2-62206-img0002.jpg)
![Flow Cytometry: CD20 Antibody (2H7) [NBP2-62206] - Surface staining of human peripheral blood (lymphocyte gate) with anti-CD20 (2H7) PE-CyTM7.](http://images.novusbio.com/fullsize/CD20-Antibody-2H7-Flow-Cytometry-NBP2-62206-img0003.jpg)
![Flow Cytometry: CD20 Antibody (2H7) [NBP2-62206] - Surface staining of human peripheral blood (lymphocyte gate) with anti-CD20 (2H7) PE-CyTM5.](http://images.novusbio.com/fullsize/CD20-Antibody-2H7-Flow-Cytometry-NBP2-62206-img0004.jpg)
![Flow Cytometry: CD20 Antibody (2H7) [NBP2-62206] - Surface staining of human peripheral blood with anti-CD20 (2H7) Alexa Fluor(R) 700.](http://images.novusbio.com/fullsize/CD20-Antibody-2H7-Flow-Cytometry-NBP2-62206-img0005.jpg)
![Flow Cytometry: CD20 Antibody (2H7) [NBP2-62206] - Surface staining of human peripheral blood with anti-CD20 (2H7) APC-CyTM7.](http://images.novusbio.com/fullsize/CD20-Antibody-2H7-Flow-Cytometry-NBP2-62206-img0006.jpg)
![Flow Cytometry: CD20 Antibody (2H7) [NBP2-62206] - Surface staining of human peripheral blood with anti-CD20 (2H7) Pacific BlueTM.](http://images.novusbio.com/fullsize/CD20-Antibody-2H7-Flow-Cytometry-NBP2-62206-img0007.jpg)
![Flow Cytometry: CD20 Antibody (2H7) [NBP2-62206] - Surface staining of human peripheral blood with anti-CD20 (2H7) PerCP-CyTM5.5.](http://images.novusbio.com/fullsize/CD20-Antibody-2H7-Flow-Cytometry-NBP2-62206-img0008.jpg)
![Flow Cytometry: CD20 Antibody (2H7) [NBP2-62206] - Surface staining of human peripheral blood with anti-CD20 (2H7) PE-DyLigt(R) 594.](http://images.novusbio.com/fullsize/CD20-Antibody-2H7-Flow-Cytometry-NBP2-62206-img0009.jpg)
![Flow Cytometry: CD20 Antibody (2H7) [NBP2-62206] - Surface staining of human peripheral blood with anti-CD20 (2H7) PerCP.](http://images.novusbio.com/fullsize/CD20-Antibody-2H7-Flow-Cytometry-NBP2-62206-img0010.jpg)
![Flow Cytometry: CD20 Antibody (2H7) [NBP2-62206] - Surface staining of human peripheral blood with anti-CD20 (2H7) APC.](http://images.novusbio.com/fullsize/CD20-Antibody-2H7-Flow-Cytometry-NBP2-62206-img0011.jpg)
![Western Blot: CD3 epsilon Antibody (C3e/1308) [NBP2-53387] - Western Blot Analysis of human Jurkat cell lysate using CD3 epsilon Antibody (C3e/1308).](http://images.novusbio.com/fullsize/CD3-epsilon-Antibody-C3e-1308-Western-Blot-NBP2-53387-img0005.jpg "Western Blot: CD3 epsilon Antibody (C3e/1308) [NBP2-53387] - Western Blot Analysis of human Jurkat cell lysate using CD3 epsilon Antibody (C3e/1308).")
![Flow Cytometry: CD3 epsilon Antibody (C3e/1308) [NBP2-53387] - Flow Cytometric Analysis of Jurkat cells using CD3 epsilon Antibody (C3e/1308) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/CD3-epsilon-Antibody-C3e-1308-Flow-Cytometry-NBP2-53387-img0004.jpg)
![Western Blot: CD3 epsilon Antibody (C3e/1308) [NBP2-53387] - Western Blot of recombinant CD3e and Jurkat Cell Lysate using CD3e Monoclonal Antibody (C3e/1308)](http://images.novusbio.com/fullsize/CD3-epsilon-Antibody-C3e-1308-Western-Blot-NBP2-53387-img0002.jpg)