| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF2418-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF3667-SP | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 647 | FAB748R-025 | |||
| Biotin | BAF748 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF748-SP | |||
| Carrier Free | FAB748R-025 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF2125-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF1002-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF1356-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF644-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF1042-SP | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Allophycocyanin | FAB1019A-025 | |||
| Allophycocyanin | FAB1019A-100 | |||
| Biotin | BAF1019 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF1019-SP | |||
| Carrier Free | FAB1019A-100 | |||
| Carrier Free | FAB1019A-025 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF971-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF447-SP | |||
![Pyruvate kinase isoform M2 (PKM2) is required for LPS-induced expression of PD-L1. RAW cells (0.5 × 106 cells/ml) (A), bone marrow-derived macrophages (BMDMs) (B), or bone marrow-derived dendritic cells (C) were treated with TEPP-46 at 50 µM for 1 h prior to stimulation with LPS (100 ng/ml, 24 h), lysed and assayed for expression of PD-L1 by flow cytometry [(A–C) top panels] and pdl1 mRNA by RT-PCR [(A,B) bottom panels]. Statistical analysis was performed on cells from three separate experiments. Error bars represent mean ± SEM. BMDM cells were transfected with scrambled control (SC) or anti-PKM2 siRNA (D). 24 h post-transfection, cells were stimulated with LPS (100 ng/ml) for 24 h after which expression of PKM2 (top panel) and PDL1 (middle panel) was measured by western blot. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29081778), licensed under a CC-BY license. Not internally tested by R&D Systems.](http://images.novusbio.com/fullsize/af1019_mouse-pd-l1-b7-h1-affinity-purified-polyclonal-ab-4120241048244.jpg)
![Immunocytochemistry/Immunofluorescence: 5-MethylCytosine Antibody (C.15200003) [NBP2-59166] - Human osteosarcoma (U2OS) cells were stained with the monoclonal antibody against 5-mC. Cells were fixed with 2.5% PFA in PBS for 30 minutes, permeabilized with 0.5% Triton X-100 for 1 hour and treated with 2N HCl for 1 hour followed by 2 x 5 minutes with 0.1 M borate buffer to depurinate the DNA. After blocking with PBS containing 0.1% Triton X-100 and 1% BSA, the cells were immunofluorescently labelled with the 5-mC antibody diluted 1:500 in blocking solution, followed by a goat anti-mouse antibody conjugated to Alexa488. Figure 3A: cells were immunofluorescently labelled with the 5-mC antibody after incubation of the antibody with 50 uM mCTP (left) or with DAPI (right). Figure 3B: staining of the cells with the 5-mC antibody after incubation of the antibody with 50 uM hmCTP and with DAPI.](http://images.novusbio.com/fullsize/5-MethylCytosine-Antibody-C-15200003-Immunofluorescence-NBP2-59166-img0005.jpg "Immunocytochemistry/Immunofluorescence: 5-MethylCytosine Antibody (C.15200003) [NBP2-59166] - Human osteosarcoma (U2OS) cells were stained with the monoclonal antibody against 5-mC. Cells were fixed with 2.5% PFA in PBS for 30 minutes, permeabilized with 0.5% Triton X-100 for 1 hour and treated with 2N HCl for 1 hour followed by 2 x 5 minutes with 0.1 M borate buffer to depurinate the DNA. After blocking with PBS containing 0.1% Triton X-100 and 1% BSA, the cells were immunofluorescently labelled with the 5-mC antibody diluted 1:500 in blocking solution, followed by a goat anti-mouse antibody conjugated to Alexa488. Figure 3A: cells were immunofluorescently labelled with the 5-mC antibody after incubation of the antibody with 50 uM mCTP (left) or with DAPI (right). Figure 3B: staining of the cells with the 5-mC antibody after incubation of the antibody with 50 uM hmCTP and with DAPI.")
![Immunocytochemistry/Immunofluorescence: 5-MethylCytosine Antibody (C.15200003) [NBP2-59166] - HeLa cells were stained with the antibody against 5-mC and with DAPI. Cells were fixed with 4% formaldehyde for 10 minutes and blocked with PBS/Triton X-100 containing 1% BSA. The cells were immunofluorescently labelled with the 5-mC antibody (middle) diluted 1:1000 in blocking solution followed by an anti-mouse antibody conjugated to Alexa594. The left panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/5-MethylCytosine-Antibody-C-15200003-Immunofluorescence-NBP2-59166-img0004.jpg)
![In-situ Hybridization: 5-MethylCytosine Antibody (C.15200003) [NBP2-59166] - To detect methylated chromosomal regions, FISH was performed on metaphase chromosomes from HeLa cells using the antibody against 5-mC. The cells were blocked in metaphase by treatment with colcemid (0.1 ug/mL) for 1 - 2 hours, fixed overnight at -20C with ethanol/glacial acetic acid and treated with 2N HCl for 30 minutes at room temperature. Subsequently, the cells were blocked with PBS containing 1% BSA and 0.1% Triton X-100 and stained with the 5-mC antibody (left) diluted 1:1,000 in blocking solution, followed by an anti-mouse antibody conjugated to Alexa594. The middle panel shows staining of the chromosomes with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/5-MethylCytosine-Antibody-C-15200003-Immunofluorescence-NBP2-59166-img0002.jpg)
![Dot Blot: 5-MethylCytosine Antibody (C.15200003) [NBP2-59166] - To demonstrate the specificity of the antibody against 5-mC, a Dot blot analysis was performed using hmC, mC and C controls. 100 to 4 ng (equivalent of 5 to 0.2 pmol of C-bases) of the controls were spotted on a membrane (Amersham Hybond-N+). The antibody was used at a dilution of 1:600. Dot blot shows a high specificity of the antibody for the methylated control.](http://images.novusbio.com/fullsize/5-MethylCytosine-Antibody-C-15200003-Dot-Blot-NBP2-59166-img0003.jpg)