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VEGFR2/KDR/Flk-1 Antibody [Biotin]

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Product Details

Summary
Applications WB, Flow, ELISA(Det)
Clonality
Polyclonal
Host
Goat
Conjugate
Biotin
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Unconjugated
AF644
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
AF644
Carrier Free
AF644
Carrier Free
AF644-SP
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VEGFR2/KDR/Flk-1 Antibody [Biotin] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse VEGFR2/KDR/Flk-1
Ala20-Glu762
Accession # P35918
Specificity
Detects mouse VEGFR2/KDR/Flk-1 in ELISAs and Western blots. In sandwich ELISAs, less than 0.5% cross-reactivity with recombinant human VEGFR2, recombinant mouse (rm) VEGF164, rmVEGFR1, rmVEGFR3, rmVEGF-B, and rmVEGF-D is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
KDR
Purity Statement
Antigen Affinity-purified
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Applications/Dilutions

Application Notes
ELISA Capture: Mouse VEGF R2/KDR/Flk-1 Antibody (Catalog # MAB4431)
ELISA Detection: Mouse VEGF R2/KDR/Flk-1 Biotinylated Antibody (Catalog # BAF644)
Standard: Recombinant Mouse VEGF R2/KDR/Flk-1 Fc Chimera (Catalog # 443-KD)

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

VEGFR2 (KDR/Flk-1), VEGFR1 (Flt-1) and VEGFR3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domains and kinase insert domains in their intracellular regions. The expression of VEGFR1, 2, and 3 is almost exclusively restricted to the endothelial cells. These receptors are likely to play essential roles in vasculogenesis and angiogenesis.

Mouse VEGFR2 cDNA encodes a 1367 amino acid (aa) residue precursor protein with a 19 aa residue signal peptide. Mature VEGFR2 is composed of a 743 aa residue extracellular domain, a 22 aa residue transmembrane domain and a 583 aa residue cytoplasmic domain. In contrast to VEGFR1 which binds both PlGF and VEGF with high affinity, VEGFR2 binds VEGF but not PlGF with high affinity. The recombinant soluble VEGFR2/Fc chimera binds VEGF with high affinity and is a potent VEGF antagonist.

  1. Ferra, N. and R. Davis-Smyth (1997) Endocrine Reviews 18:4.
  2. Achen, M.G. et al. (1998) Proc. Natl. Acad. Sci. USA 95:548.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol KDR