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TIMP-2 Antibody [Biotin]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA(Det)
Clonality
Polyclonal
Host
Goat
Conjugate
Biotin
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
Unconjugated
AF971
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
AF971
Carrier Free
AF971
Carrier Free
AF971-SP
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TIMP-2 Antibody [Biotin] Summary

Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human TIMP‑2 (R&D Systems, Catalog # 971-TM)
Cys27-Pro220
Accession # P16035
Specificity
Detects human TIMP-2 in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant human (rh) TIMP‑1, recombinant rat TIMP‑1, recombinant mouse TIMP‑1, and rhTIMP-3 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
TIMP2
Purity Statement
Antigen Affinity-purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 0.1 ug/mL
Application Notes
ELISA Capture: Human TIMP-2 Antibody (Catalog # MAB9711)
ELISA Detection: Human TIMP-2 Biotinylated Antibody (Catalog # BAF971)
Standard:Recombinant Human TIMP-2 (Catalog # 971-TM)
Readout System
Publications
Read Publications using
BAF971 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TIMP-2 Antibody [Biotin]

  • CSC-21Ktissue inhibitor of metalloproteinase 2
  • metalloproteinase inhibitor 2
  • TIMP metallopeptidase inhibitor 2
  • TIMP2
  • TIMP-2
  • Tissue inhibitor of metalloproteinases 2

Background

Tissue inhibitors of metalloproteinases or TIMPs are a family of proteins that regulate the activation and proteolytic activity of the zinc enzymes known as matrix metalloproteinases (MMPs). There are four members of the family, TIMP-1, TIMP-2, TIMP-3, and TIMP-4. TIMP-2 is a non N-glycosylated protein with a molecular mass of 22 kDa produced by a wide range of cell types, which inhibits MMPs non-covalently by the formation of binary complexes. TIMP-2 also has erythroid-potentiating and cell growth promoting activities.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TIMP-2 Antibody (BAF971)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: Antibody Array Development, ELISA Detection.


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(1)
ELISA Detection
(1)
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(2)
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Product General Protocols

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Secondary Antibodies

 

Isotype Controls

Additional TIMP-2 Products

Blogs on TIMP-2.

Check out the latest blog posts on TIMP-2.
Suppressing breast cancer metastasis: The role of hypoxia-induced RhoB expression and activation
By Jamshed Arslan, Pharm. D., PhD. The Ras homologous (Rho) GTPase family of signaling molecules has over 20 members, which typically cycle between active (GTP-bound) and inactive (GDP-bound) states. These small GTPa...  Read full blog post.
Read our latest blog and use the new citation tool on bio-techne.com

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Bioinformatics

Gene Symbol TIMP2
Uniprot