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![Immunohistochemistry: GLP-1R Antibody [NBP1-97308] - Analysis of GLP1R in mouse pancreas using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/GLP-1R-Antibody-Immunohistochemistry-NBP1-97308-img0009.jpg "Immunohistochemistry: GLP-1R Antibody [NBP1-97308] - Analysis of GLP1R in mouse pancreas using DAB with hematoxylin counterstain.")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Knockdown Validated: GLP-1R Antibody [NBP1-97308] - Liraglutide reduces apoptosis of hCMSCs via PKA/i2-catenin pathway. The expression of GLP-1R and apoptotic proteins Bax, Bcl-2, cleaved caspase-9, and cleaved caspase-3 were detected by western blot with Si-GLP-1R and liraglutide. Image collected and cropped by Citeab from the following publication (Mesenchymal stem cells combined with liraglutide relieve acute lung injury through apoptotic signaling restrained by PKA/-catenin. Stem Cell Res Ther (2020) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/GLP-1R-Antibody-Western-Blot-NBP1-97308-img0015.jpg)
![Simple Western: GLP-1R Antibody [NBP1-97308] - Simple Western lane view shows a specific band for GLP-1R in 0.5 mg/ml of Human Pancreas (left) and Mouse Pancreas (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. * Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody](http://images.novusbio.com/fullsize/GLP-1R-Antibody-Simple-Western-NBP1-97308-img0006.jpg)
![Western Blot: GLP-1R Antibody [NBP1-97308] - Total protein from Human and Mouse brain and SHSY-5Y cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-GLP1R in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/GLP-1R-Antibody-Western-Blot-NBP1-97308-img0014.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: GLP-1R Antibody [NBP1-97308] - Expression of GLP-1R in three MSCs at different time points under the stimulation of LPS. The expression of GLP-1R in hCMSCs, hBMSCs, and hAMSCs in the control group and in 30 ug/mL LPS stimulation for 24 h, 48 h, and 72 h at protein. Fluorescence intensity of GLP-1R in hCMSCs Image collected and cropped by Citeab from the following publication (Mesenchymal stem cells combined with liraglutide relieve acute lung injury through apoptotic signaling restrained by PKA/-catenin. Stem Cell Res Ther (2020) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/GLP-1R-Antibody-Western-Blot-NBP1-97308-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: GLP-1R Antibody [NBP1-97308] - GLP1R-1 antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/GLP-1R-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-97308-img0004.jpg)
![Immunohistochemistry-Paraffin: GLP-1R Antibody [NBP1-97308] - Tissue section of mouse pancreas using 1:200 dilution of rabbit anti-GLP1R antibody. The staining was developed with HRP labeled anti-rabbit IgG secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. This GLP1R antibody primarily generated a specific membrane cytoplasmic staining of apparently beta cells in the Islets of Langerhans. The cells of lobular/inter-lobular ducts and the acinar cells were largely negative for GLP1R.](http://images.novusbio.com/fullsize/GLP-1R-Antibody-Immunohistochemistry-Paraffin-NBP1-97308-img0011.jpg)
![Western Blot: GLP-1R Antibody [NBP1-97308] - Analysis of GLP1R in human pancreas cell lysate.](http://images.novusbio.com/fullsize/GLP-1R-Antibody-Western-Blot-NBP1-97308-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: GLP-1R Antibody [NBP1-97308] - Neuro2a cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-GLP-1R at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/GLP-1R-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-97308-img0012.jpg)
![Immunohistochemistry-Paraffin: GLP-1R Antibody [NBP1-97308] - Tissue section of mouse pancreas using 1:200 dilution of rabbit anti-GLP1R antibody. The staining was developed with HRP labeled anti-rabbit IgG secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. This GLP1R antibody primarily generated a specific membrane cytoplasmic staining of apparently beta cells in the Islets of Langerhans.](http://images.novusbio.com/fullsize/GLP-1R-Antibody-Immunohistochemistry-Paraffin-NBP1-97308-img0010.jpg)
![Western Blot: GLP-1R Antibody - BSA Free [NBP1-97308] - Liraglutide reduces apoptosis of hCMSCs via PKA/ beta -catenin pathway. a Western blot & b RT-qPCR verify the knockdown effects of three Si-GLP-1R in hCMSCs. c, d Western blot was used to detect of beta -catenin & p-beta -catenin expression under the stimulation of LPS by adding 20 μM H89 or 100 nM Si-GLP-1R & liraglutide. e The expression of apoptotic proteins Bax, Bcl-2, cleaved caspase-9, & cleaved caspase-3 was detected by western blot with PKA inhibitor H89 & liraglutide. f The expression of GLP-1R & apoptotic proteins Bax, Bcl-2, cleaved caspase-9, & cleaved caspase-3 were detected by western blot with Si-GLP-1R & liraglutide. Error bars represent mean ± SD from three independent experiments. Compared with Si-con group, ***P < 0.001 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32429994), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-97308_rabbit-polyclonal-glp-1r-antibody-310202415175291.jpg)
![Simple Western: GLP-1R Antibody [NLS1205] - Capillary western blot and fold change in the expression of GLP-1 receptors (GLP-1R) in the rat retina. *P < .05, significantly different from CSF group; #P < .05 significantly different from noradrenaline without exendin-4 group. Image collected and cropped by CiteAb from the following publication (//bpspubs.onlinelibrary.wiley.com/doi/10.1111/bph.15059) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/GLP-1R-Antibody-Simple-Western-NLS1205-img0015.jpg "<b>Biological Strategies Validation. </b>Simple Western: GLP-1R Antibody [NLS1205] - Capillary western blot and fold change in the expression of GLP-1 receptors (GLP-1R) in the rat retina. *P < .05, significantly different from CSF group; #P < .05 significantly different from noradrenaline without exendin-4 group. Image collected and cropped by CiteAb from the following publication (//bpspubs.onlinelibrary.wiley.com/doi/10.1111/bph.15059) licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry: GLP-1R Antibody [NLS1205] - Expression of GLP-1 receptors in human retinal microvascular endothelial cells (HRMECs). (i) Endothelial cells labelled with endothelin-1 (ET-1) antibodies (green). (j) GLP-1 receptors are labelled in red. (k) DAPI staining indicates the cell nucleus (blue). (l) Merged images. (m-p) Evaluation of GLP-1 receptors expression in human retinal pericytes (HRPs). (m) Pericytes labelled with alpha-SMA antibodies (green). (n) GLP-1 receptors are labelled in red. (o) DAPI staining indicates the cell nucleus (blue). (l and p) merged images. Image collected and cropped by CiteAb from the following publication (//bpspubs.onlinelibrary.wiley.com/doi/10.1111/bph.15059) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/GLP-1R-Antibody-Immunocytochemistry-NLS1205-img0014.jpg)
![Western Blot: GLP-1R Antibody [NLS1205] - WB detection of GLP-1R in lysates of human (1) pancreas (2) lung and (3) kidney tissues using 2 ug/mL concentration of GLP-1R antibody.](http://images.novusbio.com/fullsize/GLP-1R-Antibody-Western-Blot-NLS1205-img0012.jpg)
![Immunohistochemistry: GLP-1R Antibody [NLS1205] - Analysis of GLP-1R in mouse pancreas.](http://images.novusbio.com/fullsize/GLP-1R-Antibody-Immunohistochemistry-NLS1205-img0010.jpg)
![Immunohistochemistry: GLP-1R Antibody [NLS1205] - Human pancreas (Islets of Langerhans).](http://images.novusbio.com/fullsize/GLP-1R-Antibody-Immunohistochemistry-NLS1205-img0008.jpg)
![Simple Western: GLP-1R Antibody [NLS1205] - Image shows a specific band for GLP-1R in 0.5 mg/mL of HEK293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/GLP-1R-Antibody-Simple-Western-NLS1205-img0005.jpg)
![Microarray: GLP-1R Antibody [NLS1205] - Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma. Microarray image submitted by a verified customer review.](http://images.novusbio.com/fullsize/GLP-1R-Antibody-Microarray-NLS1205-img0013.jpg)
![Simple Western: GLP-1R Antibody - BSA Free [NLS1205] - Effect of the administration of exendin‐4 on eNOS expression. (a) Capillary western blot of total eNOS & phosphorylated eNOS expression in the rat retina. The red box indicates the target protein. (b & c) Fold change in the expression of total eNOS (b) & phosphorylated eNOS (c) in the rat retina (n = 7 in control group; n = 6 in Hp + PBS group; n = 9 in other groups). (d & e) Capillary western blot & fold change in the expression of GLP‐1 receptors (GLP‐1R) (d), PI3K, & Akt (e) in the rat retina. *P < .05, significantly different from CSF group; #P < .05significantly different from noradrenaline without exendin‐4 group. (f) NO content in human retinal microvascular endothelial cells (n = 6, 5, 5, 6, 7, 5, & 6 for group of control, OGD, OGD + Ex‐4, OGD + Ex‐4 + Ex‐9–39‐L, OGD + Ex‐4 + Ex‐9–39‐H, OGD + Ex‐4 + l‐NAME‐L, & OGD + Ex‐4 + l‐NAME‐H, respectively). One‐way ANOVA with LSD or Dunnett's T3 test were performed. C, control group; HP, high pressure injury group; EX‐4, exendin‐4; s.c., subcutaneous injection of exendin‐4; i.v., intravitreal injection of exendin‐4; od, eye drops of exendin‐4; OGD, oxygen glucose deprivation model; EX‐9–39‐L, low concentration of exendin‐9‐39 (10 nM); EX‐9–39‐H, high concentration of exendin‐9‐39 (20 nM); l‐NAME‐l, low concentration of l‐NAME (50 μM); l‐NAME‐H, high concentration of l‐NAME (100 μM) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32232832), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nls1205_rabbit-polyclonal-glp-1r-antibody-3102024161368.jpg)
![Immunohistochemistry-Paraffin: GLP-1R Antibody [NBP3-14335] - Immunohistochemistry of formalin-fixed, paraffin-embedded human ovary carcinoma after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/GLP-1R-Antibody-Immunohistochemistry-Paraffin-NBP3-14335-img0006.jpg "Immunohistochemistry-Paraffin: GLP-1R Antibody [NBP3-14335] - Immunohistochemistry of formalin-fixed, paraffin-embedded human ovary carcinoma after heat-induced antigen retrieval.")
![Immunohistochemistry-Paraffin: GLP-1R Antibody [NBP3-14335] - Immunohistochemistry of formalin-fixed, paraffin-embedded human pancreas](http://images.novusbio.com/fullsize/GLP-1R-Antibody-Immunohistochemistry-Paraffin-NBP3-14335-img0001.jpg)
![Immunohistochemistry-Paraffin: GLP-1R Antibody [NBP3-14335] - Immunohistochemistry of formalin-fixed, paraffin-embedded human adrenal, pheochromocytoma, after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/GLP-1R-Antibody-Immunohistochemistry-Paraffin-NBP3-14335-img0002.jpg)
![Immunohistochemistry-Paraffin: GLP-1R Antibody [NBP3-14335] - Immunohistochemistry of formalin-fixed, paraffin-embedded human lung, small cell carcinoma, after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/GLP-1R-Antibody-Immunohistochemistry-Paraffin-NBP3-14335-img0003.jpg)
![Immunohistochemistry-Paraffin: GLP-1R Antibody [NBP3-14335] - Immunohistochemistry of formalin-fixed, paraffin-embedded human pancreas, islet of Langerhans, after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/GLP-1R-Antibody-Immunohistochemistry-Paraffin-NBP3-14335-img0004.jpg)
![Immunohistochemistry-Paraffin: GLP-1R Antibody [NBP3-14335] - Immunohistochemistry of formalin-fixed, paraffin-embedded human skin melanoma after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/GLP-1R-Antibody-Immunohistochemistry-Paraffin-NBP3-14335-img0005.jpg)
![Immunohistochemistry: GLP-1R Antibody [NBP3-14335] - Immunohistochemistry of adrenal](http://images.novusbio.com/fullsize/GLP-1R Antibody-Immunohistochemistry-NBP3-14335-img0007.jpg)
![Immunohistochemistry-Paraffin: GLP-1R Antibody (HL2297) - Azide and BSA Free [NBP3-25482] - GLP1R antibody [HL2297] detects GLP1R protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded rat pancreas. GLP1R stained by GLP1R antibody [HL2297] (NBP3-25482) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25482_rabbit-glp-1r-mab-hl2297-azide-and-bsa-free-2120249371536.jpg "Immunohistochemistry-Paraffin: GLP-1R Antibody (HL2297) - Azide and BSA Free [NBP3-25482] - GLP1R antibody [HL2297] detects GLP1R protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded rat pancreas. GLP1R stained by GLP1R antibody [HL2297] (NBP3-25482) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunohistochemistry-Paraffin: GLP-1R Antibody (HL2297) - Azide and BSA Free [NBP3-25482] - GLP1R antibody [HL2297] detects GLP1R protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded mouse pancreas. GLP1R stained by GLP1R antibody [HL2297] (NBP3-25482) diluted at 1:20000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25482_rabbit-glp-1r-mab-hl2297-azide-and-bsa-free-2120249422618.jpg)
![Immunohistochemistry-Paraffin: GLP-1R Antibody (HL2297) - Azide and BSA Free [NBP3-25482] - GLP1R antibody [HL2297] detects GLP1R protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded mouse pancreas. GLP1R stained by GLP1R antibody [HL2297] (NBP3-25482) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25482_rabbit-glp-1r-mab-hl2297-azide-and-bsa-free-212024941440.jpg)
![Immunohistochemistry-Paraffin: GLP-1R Antibody (HL2297) - Azide and BSA Free [NBP3-25482] - GLP1R antibody [HL2297] detects GLP1R protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded human pancreas. GLP1R stained by GLP1R antibody [HL2297] (NBP3-25482) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25482_rabbit-glp-1r-mab-hl2297-azide-and-bsa-free-2120249413388.jpg)
![Western Blot: GLP-1R Antibody (HL2297) - Azide and BSA Free [NBP3-25482] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with GLP-1R antibody [HL2297] (NBP3-25482) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25482_rabbit-glp-1r-mab-hl2297-azide-and-bsa-free-9102024205634.jpg)
![Western Blot: GLP-1R Antibody (HL2298) - Azide and BSA Free [NBP3-25483] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with GLP1R antibody [HL2298] (NBP3-25483) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25483_rabbit-glp-1r-mab-hl2298-azide-and-bsa-free-21202494088.jpg "Western Blot: GLP-1R Antibody (HL2298) - Azide and BSA Free [NBP3-25483] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with GLP1R antibody [HL2298] (NBP3-25483) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: GLP-1R Antibody (HL2298) - Azide and BSA Free [NBP3-25483] - GLP1R antibody [HL2298] detects GLP1R protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded mouse pancreas. GLP1R stained by GLP1R antibody [HL2298] (NBP3-25483) diluted at 1:20000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25483_rabbit-glp-1r-mab-hl2298-azide-and-bsa-free-2120249342743.jpg)
![ELISA: Human GLP-1R ELISA Kit (Colorimetric) [NBP2-82165] - Samples were spiked with high concentrations of Human GLP-1R and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-82165_human-glp-1r-elisa-kit-colorimetric-132202416121315.jpg "ELISA: Human GLP-1R ELISA Kit (Colorimetric) [NBP2-82165] - Samples were spiked with high concentrations of Human GLP-1R and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human GLP-1R ELISA Kit (Colorimetric) [NBP2-82165] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-82165_human-glp-1r-elisa-kit-colorimetric-432024855062.jpg)