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H00001070-M01

Centrin 3 Antibody (3E6)

(1 Review)

(43 Publications)

50 μg

H00001070-M01

Western Blot: Centrin 3 Antibody (3E6) [H00001070-M01] - Analysis of 293T cells using Centrin 3 antibody (3E6). Image from verified customer review.

Western Blot: Centrin 3 Antibody (3E6) [H00001070-M01] - CETN3 monoclonal antibody (M01), clone 3E6 Analysis of CETN3 expression in Jurkat.

Western Blot: Centrin 3 Antibody (3E6) [H00001070-M01] - Analysis of CETN3 expression in transfected 293T cell line by CETN3 monoclonal antibody (M01), clone 3E6.Lane 1: CETN3 transfected lysate(19.5 KDa).Lane 2: Non-transfected lysate.

Western Blot: Centrin 3 Antibody (3E6) [H00001070-M01] - Centrin 3 Antibody (3E6) [H00001070-M01] - Analysis of CETN3 over-expressed 293 cell line, cotransfected with CETN3 Validated Chimera RNAi ( Cat # H00001070-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CETN3 monoclonal antibody (M01), clone 3E6 (Cat # H00001070-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

ELISA: Centrin 3 Antibody (3E6) [H00001070-M01] - Centrin 3 Antibody (3E6) [H00001070-M01] - Detection limit for recombinant GST tagged CETN3 is approximately 0.1ng/ml as a capture antibody.

Reactivity:

Hu, Mu, Rt, Ch, Ze

Species Glossary

Reactivity: Hu, Mu, Rt, Ch, Ze

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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NB110-55376

TDP-43/TARDBP Antibody - BSA Free

(1 Review)

(13 Publications)

0.1 ml

NB110-55376

Western Blot: TDP-43/TARDBP Antibody [NB110-55376] - Total protein from HeLa, MCF7 and mouse brain was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-TARDBP in 1% block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.

Immunocytochemistry/Immunofluorescence: TDP-43/TARDBP Antibody [NB110-55376] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with TDP-43/TARDBP Antibody conjugated to Alexa Fluor 488 (NB110-55376AF488) at 10 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Immunocytochemistry/Immunofluorescence: TDP-43/TARDBP Antibody [NB110-55376] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton X-100. The cells were incubated with anti-TARDBP at 2 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunohistochemistry-Paraffin: TDP-43/TARDBP Antibody [NB110-55376] - Analysis of FFPE human placenta using TDP-43 antibody at 1:250 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Nuclear staining was observed. Staining was performed by Histowiz.

Western Blot: TDP-43/TARDBP Antibody [NB110-55376] - TARDBP antibody was tested in HeLa WCE.

Western Blot: TDP-43/TARDBP Antibody [NB110-55376] - Cells were transfected with the pCMV6-ENTRY control or pCMV6-ENTRY TARDBP cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TARDBP.

Immunocytochemistry/Immunofluorescence: TDP-43/TARDBP Antibody [NB110-55376] - TARDBP antibody was tested in MCF-7 cells with DyLight 488 (Green). Nuclei and alpha-tubulin were counterstained with DAPI (Blue) and DyLight 550 (Red).

Immunocytochemistry/Immunofluorescence: TDP-43/TARDBP Antibody [NB110-55376] - Analysis using the DyLight 550 conjugate of NB110-55376. Staining of Human iPS derived neurons, fixed in 4% formaldehyde solution. NB110-55376R was diluted 1:200. All antibodies were diluted in PBS/BSA 3% (w/v) ) Triton X-100 0,3 % (v/v). Images taken by an Arrayscan (Cellomics). Blue dots in TDP staining is Hoechst.

Immunocytochemistry/Immunofluorescence: TDP-43/TARDBP Antibody [NB110-55376] - Subcellular distribution of TDP-43 and CWC22 in control DRG sensory neurons. TDP-43 was stained diffusely in the nucleus, excluding SMN foci in sensory neurons. Scale bar: 10 um. Image collected and cropped by CiteAb from the following publication (//dmm.biologists.org/lookup/doi/10.1242/dmm.028225) licensed under a CC-BY license.

Reactivity:

Hu, Mu, Ch, Pm, Xp, Ze

Species Glossary

Reactivity: Hu, Mu, Ch, Pm, Xp, Ze

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB110-55376AF350
Alexa Fluor 405	NB110-55376AF405
Alexa Fluor 488	NB110-55376AF488
Alexa Fluor 532	NB110-55376AF532
Alexa Fluor 594	NB110-55376AF594
Alexa Fluor 647	NB110-55376AF647
Alexa Fluor 700	NB110-55376AF700
Alexa Fluor 750	NB110-55376AF750
Allophycocyanin	NB110-55376APC
Biotin	NB110-55376B
DyLight 350	NB110-55376UV
DyLight 405	NB110-55376V
DyLight 488	NB110-55376G
DyLight 550	NB110-55376R
DyLight 594	NB110-55376DL594
DyLight 650	NB110-55376C
DyLight 680	NB110-55376FR
DyLight 755	NB110-55376IR
FITC	NB110-55376F
HRP	NB110-55376H
Janelia Fluor 549	NB110-55376JF549
Janelia Fluor 646	NB110-55376JF646
PE	NB110-55376PE
PerCP	NB110-55376PCP
mFluor Violet 450 SE	NB110-55376MFV450
mFluor Violet 500 SE	NB110-55376MFV500
mFluor Violet 610 SE	NB110-55376MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB110-55376SS

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NBP1-81445

CEP164 Antibody

(17 Publications)

0.1 ml

NBP1-81445

Immunocytochemistry/Immunofluorescence: CEP164 Antibody [NBP1-81445] - Fetuin-A inhibits ciliogenesis. Primary cilia were examined in the absence (CTL) or presence of fetuin-A (FA) by immunostaining with antibodies against acetylated tubulin (Ace-tub), Cep164, IFT88, or Arl13b. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/1422-0067/20/20/5207), licensed under a CC-BY license.

Immunohistochemistry-Paraffin: CEP164 Antibody [NBP1-81445] - Staining of human fallopian tube shows strong positivity in cilia in glandular cells.

Immunocytochemistry/Immunofluorescence: CEP164 Antibody [NBP1-81445] - Staining of human cell line U-2 OS shows localization to centrosome. Antibody staining is shown in green.

Immunocytochemistry/Immunofluorescence: CEP164 Antibody [NBP1-81445] - CEP295 overexpression induces extra-long microtubule-based filaments with low efficiency. Schematics on right of D indicate centrioles (black rectangles) and positions of CEP164 (red squares). MC, mother centriole; DC, daughter centriole. Image collected and cropped by CiteAb from the following publication (//jcs.biologists.org/lookup/doi/10.1242/jcs.186338), licensed under a CC-BY license.

Immunohistochemistry-Paraffin: CEP164 Antibody [NBP1-81445] - Staining of human skeletal muscle shows no positivity in myocytes as expected.

Immunohistochemistry-Paraffin: CEP164 Antibody [NBP1-81445] - Staining of human bronchus shows moderate to strong positivity in cilia in respiratory epithelial cells.

Immunohistochemistry-Paraffin: CEP164 Antibody [NBP1-81445] - Staining of human nasopharynx shows strong positivity in cilia in respiratory epithelial cells.

Immunohistochemistry-Paraffin: CEP164 Antibody [NBP1-81445] - Staining of human endometrium shows strong positivity in cilia in glandular cells.

Fetuin-A inhibits ciliogenesis. (A–D) Primary cilia were examined in the absence (CTL) or presence of fetuin-A (FA) by immunostaining with antibodies against acetylated tubulin (Ace-tub), Cep164, IFT88, or Arl13b. (E) Quantification results of the frequency of ciliated HTR8 cells. ** p < 0.01, results are the mean ± SD from three independent experiments, more than 100 cells were counted in each individual group. DNA was stained with DAPI. Scale bar 10 µM.

CEP295 overexpression induces extra-long microtubule-based filaments with low efficiency. (D). Schematics on right of D indicate centrioles (black rectangles) and positions of CEP164 (red squares). MC, mother centriole; DC, daughter centriole. (E) The quantitation of percentage of CEP164 signal at elongated centrioles in G-CEP295R-inducible cells. Error bars represent the mean+/-s.e.m. from two independent experiments. The total number n as indicated. Image collected and cropped by CiteAb from the following publication (//journals.biologists.com/jcs/article/doi/10.1242/jcs.186338/260194/CEP295-interacts-with-microtubules-and-is-required), licensed under a CC-BY licence.

CPAP-E1235V mutation impaired the recruitment of centriole elongation proteins onto the centrioles. Cells were synchronized in the early S phase by treatment with aphidicolin (2 ug/ml) for 24 h. They were then fixed immediately with methanol (S phase centrioles) or released into fresh culture medium without aphidicolin for another 16 h (G2 phase centrioles). CPAP-WT or mutant cells in the S phase or G2 phase were immunostained with antibodies against the following centriolar proteins. (A) STIL, n = 42 for CPAP-WT; n = 25 for CPAP-E1235V#1; n = 34 for CPAP-E1235V#2. (B) CEP120, n = 50 for CPAP-WT; n = 42 for CPAP-E1235V#1; n = 48 for CPAP-E1235V#2. (C) CENTROBIN, n = 40 for all groups. (D) CEP295, n = 40 for all groups. (E) POC5, n = 42 for CPAP-WT; n = 40 for CPAP-E1235V#1; n = 40 for CPAP-E1235V#2. (F) POC1B, n = 40 for all groups. (G) CEP164, n = 88 for CPAP-WT; n = 74 for CPAP-E1235V#1; n = 38 for CPAP-E1235V#2. All data are presented as mean +/-SEM from a pool of n cells from three independent experiments. n.s: not significant; **p < 0.01; ***p < 0.001. Scale bar: 5 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35309908), licensed under a CC-BY licence.

Reactivity:

Hu, Mu, Ze

Species Glossary

Reactivity: Hu, Mu, Ze

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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NB500-138

p38 alpha [p Thr180, p Tyr182] Antibody

(17 Publications)

0.1 ml

NB500-138

Western Blot: p38 [p Thr180, p Tyr182] Antibody [NB500-138] - Hela cell lysates that had been treated with UV or untreated (Control) showing specific immunolabeling of the -39 kDa p38 MAPK protein phosphorylated at Thr180/Tyr182.

Immunohistochemistry-Paraffin: p38 [p Thr180, p Tyr182] Antibody [NB500-138] - Immunostaining of human breast cancer tissue showing p38 when phosphorylated at Thr180/Tyr182 in red.

Western Blot: p38 [p Thr180, p Tyr182] Antibody [NB500-138] - Western blot of HeLa cell lysates that had been treated with UV or untreated (Control) showing specific immunolabeling of the ~39k p38 MAPK protein phosphorylated at Thr180/Tyr182.

Reactivity:

Hu, Mu, Ze, Rt, Bv, Ca, Ch, Pm

Species Glossary

Reactivity: Hu, Mu, Ze, Rt, Bv, Ca, Ch, Pm

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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NBP2-24695

Cyclin D1 Antibody

(4 Publications)

0.1 mg

NBP2-24695

Western Blot: Cyclin D1 Antibody [NBP2-24695] - Analysis of 1) mouse C2C12 and 2) mouse NIH 3T3 lysate using Cyclin D1 antibody at 5 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Immunohistochemistry-Paraffin: Cyclin D1 Antibody [NBP2-24695] - Formalin-fixed, paraffin-embedded human small intestine stained with Cyclin D1 antibody at 10 ug/ml.

Reactivity:

Hu, Mu, Bv, Ca, Ch, Pm, Eq, Pm-Or, Ze

Species Glossary

Reactivity: Hu, Mu, Bv, Ca, Ch, Pm, Eq, Pm-Or, Ze

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Alt Catalog#

IMG-6583A

Alt Catalog# IMG-6583A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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NBP2-16396

Ezrin Antibody

(1 Review)

(3 Publications)

0.1 ml

NBP2-16396

Western Blot: Ezrin Antibody [NBP2-16396] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Ezrin antibody [N2C2], Internal diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody

Immunocytochemistry/Immunofluorescence: Ezrin Antibody [NBP2-16396] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Ezrin stained by Ezrin antibody [N2C2], Internal diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [114] diluted at 1:1000.Blue: Fluoroshield with DAPI.

Immunohistochemistry-Paraffin: Ezrin Antibody [NBP2-16396] - Rat duodenum. Ezrin antibody [N2C2], Internal diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Immunocytochemistry/Immunofluorescence: Ezrin Antibody [NBP2-16396] - HCT116 cells.

Immunohistochemistry-Paraffin: Ezrin Antibody [NBP2-16396] - C2C12 xenograft, using Ezrin antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunohistochemistry-Paraffin: Ezrin Antibody [NBP2-16396] - Human colon carcinoma, using Ezrin antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

IHC-Whole-Mount- Ezrin Antibody [NBP2-16396]--Fixed zebrafish embryos. Ezrin antibody [N2C2], Internal dilution: 1:200.

IHC-Whole-Mount- Ezrin Antibody [NBP2-16396] - Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo. Green: Ezrin stained by Ezrin antibody [N2C2], Internal diluted at 1:100. Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70C

Western Blot: Ezrin Antibody [NBP2-16396] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Ezrin antibody [N2C2], Internal diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301)) was used to detect the primary antibody

Western Blot: Ezrin Antibody [NBP2-16396] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Ezrin antibody [N2C2], Internal diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Reactivity:

Hu, Mu, Rt, Ze, Bv, Ca, Ch, Pm, Rb

Species Glossary

Reactivity: Hu, Mu, Rt, Ze, Bv, Ca, Ch, Pm, Rb

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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NBP2-22151

Histone Deacetylase 4/HDAC4 Antibody - BSA Free

(5 Publications)

0.1 ml

NBP2-22151

Western Blot: Histone Deacetylase 4/HDAC4 Antibody [NBP2-22151] - Expression of HDACs in murine optic nerve. Western blot analysis of HDAC proteins in E16, P5 and P30 murine optic nerve was performed to check the specificity of the antibodies used for the study. Blots were normalized to beta-TUBULIN. Densitometric graphs for each HDAC panel show quantification of protein levels expressed as fold-change from E16. p < 0.05 was considered statistically significant and represented by a single *, whereas p < 0.005 is represented by **. Bars show mean +/- SEM of three samples per timepoint. Note that the apparent increase in band intensities for some P5 samples reflect increased protein loading in those lanes. Image collected and cropped by CiteAb from the following publication (//bmcdevbiol.biomedcentral.com/articles/10.1186/1471-213X-14-30), licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: Histone Deacetylase 4/HDAC4 Antibody [NBP2-22151] - HDAC4 antibody was tested in HeLa cells at a 1:100 dilution against DyLight 488 (Green) using a 40X objective. Alpha tubulin and nuclei were counterstained against DyLight 568 (Red) and DAPI (Blue), respectively.

Immunohistochemistry: Histone Deacetylase 4/HDAC4 Antibody [NBP2-22151] - HDAC4 localization pattern in developing murine optic nerve. Optic nerve sections at P30 were triple-labeled with HDAC4 (red). Double-label of SOX2 and HDAC4. Composite image in M show z stacks of optic nerve at high magnification; horizontal yellow lines correspond the x axis plane and vertical yellow lines corresponds to the y axis. The x, z axis is shown at the bottom of each panel, while the y, z axis is shown to the right of the panel. Abbreviations: R; retina, ON; optic nerve. Scale bars = 50 um. Image collected and cropped by CiteAb from the following publication (//bmcdevbiol.biomedcentral.com/articles/10.1186/1471-213X-14-30), licensed under a CC-BY license.

Western Blot: Histone Deacetylase 4/HDAC4 Antibody [NBP2-22151] - Analysis of HDAC4 in NIH/3T3 lysate.

Immunohistochemistry: Histone Deacetylase 4/HDAC4 Antibody [NBP2-22151] - Analysis of HDAC4 in mouse pancreas.

Simple Western: Histone Deacetylase 4/HDAC4 Antibody [NBP2-22151] - Image shows a specific band for HDAC4 in 0.5 mg/mL of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Reactivity:

Hu, Mu, Rt, Pm, Ch, Ze

Species Glossary

Reactivity: Hu, Mu, Rt, Pm, Ch, Ze

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-22151AF350
Alexa Fluor 405	NBP2-22151AF405
Alexa Fluor 488	NBP2-22151AF488
Alexa Fluor 532	NBP2-22151AF532
Alexa Fluor 594	NBP2-22151AF594
Alexa Fluor 647	NBP2-22151AF647
Alexa Fluor 700	NBP2-22151AF700
Alexa Fluor 750	NBP2-22151AF750
Allophycocyanin	NBP2-22151APC
Biotin	NBP2-22151B
DyLight 350	NBP2-22151UV
DyLight 405	NBP2-22151V
DyLight 488	NBP2-22151G
DyLight 550	NBP2-22151R
DyLight 594	NBP2-22151DL594
DyLight 650	NBP2-22151C
DyLight 680	NBP2-22151FR
DyLight 755	NBP2-22151IR
FITC	NBP2-22151F
HRP	NBP2-22151H
Janelia Fluor 549	NBP2-22151JF549
Janelia Fluor 646	NBP2-22151JF646
PE	NBP2-22151PE
PerCP	NBP2-22151PCP
mFluor Violet 450 SE	NBP2-22151MFV450
mFluor Violet 500 SE	NBP2-22151MFV500
mFluor Violet 610 SE	NBP2-22151MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-22151-0.025ml

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H00004218-M02

RAB8A Antibody (3G1)

(1 Review)

(9 Publications)

0.1 mg

H00004218-M02

Western Blot: RAB8A Antibody (3G1) [H00004218-M02] - RAB8A monoclonal antibody (M02), clone 3G1 Analysis of RAB8A expression in Hela S3 NE.

Immunocytochemistry/Immunofluorescence: RAB8A Antibody (3G1) [H00004218-M02] - Neuromast hair cells of zebrafish embryos stained with RAB8A antibody. Donkey anti-mouse Alexa Fluor 594 secondary antibody was used. Image from verified customer.

ELISA: RAB8A Antibody (3G1) [H00004218-M02] - Detection limit for recombinant GST tagged RAB8A is approximately 0.1ng/ml as a capture antibody.

Reactivity:

Hu, Mu, Ze

Species Glossary

Reactivity: Hu, Mu, Ze

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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NBP1-31661

RRM2 Antibody

(1 Review)

(3 Publications)

100 ul

NBP1-31661

Immunocytochemistry/Immunofluorescence: RRM2 Antibody [NBP1-31661] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: RRM2 protein stained by RRM2 antibody [N1C1] diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.

Immunohistochemistry-Paraffin: RRM2 Antibody [NBP1-31661] - Mouse tumor tissues were stained with RRM2 antibody at 1:50 dilution. Image submitted by a verified customer review.

Immunocytochemistry/Immunofluorescence: RRM2 Antibody [NBP1-31661] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: RRM2 protein stained by RRM2 antibody [N1C1] diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar = 10 um.

Immunohistochemistry-Paraffin: RRM2 Antibody [NBP1-31661] - Mouse muscle. RRM2 antibody [N1C1] diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunohistochemistry-Paraffin: RRM2 Antibody [NBP1-31661] - Rat intestine. RRM2 antibody [N1C1] iluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry-Paraffin: RRM2 Antibody [NBP1-31661] - Mouse muscle. RRM2 antibody [N1C1] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Western Blot: RRM2 Antibody [NBP1-31661] - Various tissue extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RRM2 antibody [N1C1] diluted at 1:1000.

Reactivity:

Hu, Mu, Rt, Ze, Bv

Species Glossary

Reactivity: Hu, Mu, Rt, Ze, Bv

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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NBP1-31336

Fumarase Antibody

(1 Review)

(1 Publication)

0.1 ml

NBP1-31336

Knockdown Validated: Fumarase Antibody [NBP1-31336] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Fumarate hydratase antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

<b>Genetic Strategies Validation. </b>Western Blot: Fumarase Antibody [NBP1-31336] - Representative western blots showing protein expression of fumarase and key enzymes in OXPHOS. VDAC was used as a loading control (n = 3). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep22788) licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: Fumarase Antibody [NBP1-31336] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Fumarate hydratase protein stained by Fumarate hydratase antibody diluted at 1:500.Blue: Hoechst 33342 staining.

Immunohistochemistry-Paraffin: Fumarase Antibody [NBP1-31336] - Analysis of paraffin-embedded NCIN87 xenograft, using fumarate hydratase antibody at 1:500 dilution.

Western Blot: Fumarase Antibody [NBP1-31336] - A. 50 ug mouse liver lysate/extract 7.5% SDS-PAGE Fumarate hydratase antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Western Blot: Fumarase Antibody [NBP1-31336] - A.50 ug rat liver lysate/extract 10% SDS-PAGE Fumarate hydratase antibody dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Western Blot: Fumarase Antibody [NBP1-31336] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Fumarate hydratase antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

IR-Mut iPSC have decreased PGC1alpha expression.(a) Representative western blots showing protein expression of fumarase and key enzymes in OXPHOS. VDAC was used as a loading control (n = 3). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26948272), licensed under a CC-BY licence.

Reactivity:

Hu, Mu, Rt, Po, Bv, Ch, Pm, Sh, Ze

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Bv, Ch, Pm, Sh, Ze

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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NBP2-27202

14-3-3 Antibody

(1 Review)

0.1 mg

NBP2-27202

Western Blot: 14-3-3 Antibody [NBP2-27202] - analysis of 14-3-3 using 14-3-3 antibody. Human Brain tissue lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse brain, and 4) rat brain lysate probed with 2 ug/ml of 14-3-3 antibody. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Immunocytochemistry/Immunofluorescence: 14-3-3 Antibody [NBP2-27202] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-14-3-3 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Simple Western: 14-3-3 Antibody [NBP2-27202] - Simple Western lane view shows a specific band for 14-3-3 Gamma in 0.5 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Simple Western: 14-3-3 Antibody [NBP2-27202] - Analysis in mouse brain lysate. Image from verified customer review.

Reactivity:

Hu, Mu, Rt, Po, Ca, Ch, ChHa, Fe, Ma-Op, Pm, Am, Bv, Pm, Pm-Or, Re, Ze

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Ca, Ch, ChHa, Fe, Ma-Op, Pm, Am, Bv, Pm, Pm-Or, Re, Ze

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Alt Catalog#

IMG-6796A

Alt Catalog# IMG-6796A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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NB110-40823

BMI-1 Antibody

(2 Publications)

0.1 ml

NB110-40823

Western Blot: Bmi1 Antibody [NB110-40823] - Detection of Bmi1 protein in skeletal muscle

Immunocytochemistry/Immunofluorescence: Bmi1 Antibody [NB110-40823] - BMI1 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Reactivity:

Hu, Mu, Rt, Bv, Ch, Fe, Pm, Rb, Ze

Species Glossary

Reactivity: Hu, Mu, Rt, Bv, Ch, Fe, Pm, Rb, Ze

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB110-40823AF350
Alexa Fluor 405	NB110-40823AF405
Alexa Fluor 488	NB110-40823AF488
Alexa Fluor 532	NB110-40823AF532
Alexa Fluor 594	NB110-40823AF594
Alexa Fluor 647	NB110-40823AF647
Alexa Fluor 700	NB110-40823AF700
Alexa Fluor 750	NB110-40823AF750
Allophycocyanin	NB110-40823APC
Biotin	NB110-40823B
DyLight 350	NB110-40823UV
DyLight 405	NB110-40823V
DyLight 488	NB110-40823G
DyLight 550	NB110-40823R
DyLight 594	NB110-40823DL594
DyLight 650	NB110-40823C
DyLight 680	NB110-40823FR
DyLight 755	NB110-40823IR
FITC	NB110-40823F
HRP	NB110-40823H
Janelia Fluor 549	NB110-40823JF549
Janelia Fluor 646	NB110-40823JF646
PE	NB110-40823PE
PerCP	NB110-40823PCP
mFluor Violet 450 SE	NB110-40823MFV450
mFluor Violet 500 SE	NB110-40823MFV500
mFluor Violet 610 SE	NB110-40823MFV610

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