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ZEB2 Antibody (CL13467)

Images

 
Staining of human cervix shows strong nuclear positivity in squamous epithelial cells.
Staining of human tonsil shows strong nuclear positivity in germinal center cells.
Staining of human anaplastic oligodendroglioma shows strong nuclear positivity in tumor cells.
Staining of human skeletal muscle shows no positivity in myocytes as expected.
Staining in U-87 cell line with Anti-ZEB2 monoclonal antibody, showing specific staining of nuclear speckles in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).
Orthogonal Strategies: Analysis in human cerebral cortex and skeletal muscle tissues using NBP3-24559 antibody. Corresponding ZEB2 RNA-seq data are presented for the same tissues.
Staining of human cerebral cortex shows strong nuclear positivity in neurons.
Orthogonal Strategies: Western blot analysis in human cell lines SK-MEL-30 and Caco-2 using Anti-ZEB2 antibody. Corresponding ZEB2 RNA-seq data are presented for the same cell lines. Loading control: Anti-HDAC1.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
CL13467
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

ZEB2 Antibody (CL13467) Summary

Immunogen
This antibody was designed to target the recombinant protein ZEB2, with the precise immunogen sequence being withheld for proprietary reasons.
Predicted Species
Mouse (93%), Rat (95%). Backed by our 100% Guarantee.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ZEB2
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 1ug/ml
Application Notes
For IHC-Paraffin, we recommend using Heat-Induced Epitope Retrieval (HIER) with a pH level of 6. This optimized retrieval method ensures the best results for your experiments, For ICC/IF, we recommend using a combination of PFA and Triton X-100. This will give you the optimal results for your experiments.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, pH 7.2, 40% glycerol
Preservative
0.02% Sodium Azide
Purity
Protein A purified

Alternate Names for ZEB2 Antibody (CL13467)

  • HSPC082
  • KIAA0569FLJ42816
  • SIP1
  • SIP1HSPC082
  • Smad-interacting protein 1
  • SMADIP1
  • SMADIP1ZFHX1BSIP-1
  • ZEB2
  • ZFHX1B
  • ZFX1B
  • zinc finger E-box binding homeobox 2
  • zinc finger E-box-binding homeobox 2
  • zinc finger homeobox 1b
  • Zinc finger homeobox protein 1b

Background

The ZEB2 gene (also known as SIP1 and SMADIP1) is a member of the delta-EF1 (ZEB1; MIM 189909)/Zfh1 family of 2-handed zinc finger/homeodomain proteins. SMADIP1 interacts with receptor-mediated, activated full-length SMADs (see MIM 605568) (Verschueren et al., 1999 [PubMed 10400677]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ZEB2 Antibody (NBP3-24559) (0)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ZEB2 Antibody (NBP3-24559) (0)

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Secondary Antibodies

 

Isotype Controls

Additional ZEB2 Products

Array NBP3-24559

Research Areas for ZEB2 Antibody (NBP3-24559)

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Blogs on ZEB2

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Bioinformatics

Gene Symbol ZEB2