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WT1 Antibody (CL13170)

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Western Blot: WT1 Antibody (CL13170) [NBP3-18547] - Analysis in human cell line HEK 293 and human cell line SK-MEL-30.
Orthogonal Strategies: Western Blot: WT1 Antibody (CL13170) [NBP3-18547] - Analysis in human cell line HEK 293 and human cell line SK-MEL-30.
Immunocytochemistry/ Immunofluorescence: WT1 Antibody (CL13170) [NBP3-18547] - Staining of CACO-2 cells using the Anti-WT1 monoclonal antibody, showing specific staining in nucleoplasm in green. Microtubule probes are ...read more
Immunohistochemistry-Paraffin: WT1 Antibody (CL13170) [NBP3-18547] - Staining of human prostate shows no positivity in glandular cells as expected.
Immunohistochemistry-Paraffin: WT1 Antibody (CL13170) [NBP3-18547] - Analysis in human testis and prostate tissues using NBP3-18547 antibody. Corresponding WT1 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: WT1 Antibody (CL13170) [NBP3-18547] - Staining of human ovarian cancer (endometrioid carcinoma) shows strong nuclear positivity in tumor cells.
Immunohistochemistry-Paraffin: WT1 Antibody (CL13170) [NBP3-18547] - Staining of human ovarian cancer (high grade serous carcinoma) shows strong nuclear positivity in tumor cells.
Immunohistochemistry-Paraffin: WT1 Antibody (CL13170) [NBP3-18547] - Staining of human ovarian cancer (papillary carcinoma) shows strong nuclear positivity in tumor cells.
Immunohistochemistry-Paraffin: WT1 Antibody (CL13170) [NBP3-18547] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: WT1 Antibody (CL13170) [NBP3-18547] - Staining of human fallopian tube shows strong nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: WT1 Antibody (CL13170) [NBP3-18547] - Staining of human kidney shows strong nuclear positivity in cells in glomeruli.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
CL13170
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

  

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP3-18547AF350
Alexa Fluor 405
NBP3-18547AF405
Alexa Fluor 488
NBP3-18547AF488
Alexa Fluor 532
NBP3-18547AF532
Alexa Fluor 594
NBP3-18547AF594
Alexa Fluor 647
NBP3-18547AF647
Alexa Fluor 700
NBP3-18547AF700
Alexa Fluor 750
NBP3-18547AF750
Allophycocyanin
NBP3-18547APC
Biotin
NBP3-18547B
CoraFluor 1
NBP3-18547CL1
DyLight 350
NBP3-18547UV
DyLight 405
NBP3-18547V
DyLight 488
NBP3-18547G
DyLight 550
NBP3-18547R
DyLight 594
NBP3-18547DL594
DyLight 650
NBP3-18547C
DyLight 680
NBP3-18547FR
DyLight 755
NBP3-18547IR
FITC
NBP3-18547F
HRP
NBP3-18547H
Janelia Fluor 525
NBP3-18547JF525
Janelia Fluor 549
NBP3-18547JF549
Janelia Fluor 585
NBP3-18547JF585
Janelia Fluor 635
NBP3-18547JF635
Janelia Fluor 646
NBP3-18547JF646
Janelia Fluor 669
NBP3-18547JF669
PE
NBP3-18547PE
PerCP
NBP3-18547PCP
mFluor Violet 450 SE
NBP3-18547MFV450
mFluor Violet 500 SE
NBP3-18547MFV500
mFluor Violet 610 SE
NBP3-18547MFV610
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP3-44378
Novus Biologicals offers 100% Guarantee

WT1 Antibody (CL13170) Summary

Immunogen
This antibody was developed against a sythetic peptide for P19544. Actual immunogen sequence is proprietary.
Predicted Species
Mouse (96%), Rat (96%). Backed by our 100% Guarantee.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
WT1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:200-1:500
  • Western Blot 1 ug/ml
Application Notes
For use with IHC-Paraffin, HIER pH 6 retrieval is recommended. For use with ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, pH 7.2, 40% glycerol
Preservative
0.02% Sodium Azide
Purity
Protein A purified

Alternate Names for WT1 Antibody (CL13170)

  • AWT1
  • GUD
  • NPHS4GUD
  • WAGR
  • Wilms tumor 1
  • Wilms tumor protein
  • WIT-2
  • WT1
  • WT33

Background

Wilms' tumor protein (WT1) is a transcription factor which regulates the expression of numerous target genes, including EPO. This protein plays an essential role in the normal development of the urogenital system, including cellular development and cell survival, and it has both tumor suppressor and oncogenic roles in tumor formation.

Defects in the WT1 protein can lead to Wilms tumor, Frasier syndrome, Denys-Drash syndrome, nephrotic syndrome type 4, and Meacham syndrome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

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ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol WT1