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VSIG3/IGSF11 Antibody (774208) [Unconjugated]

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HEK293 human embryonic kidney cell line transfected with either (A) human VSIG3 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human VSIG3 Monoclonal Antibody (Catalog # MAB9229) followed by APC-conjugated Goat-anti Mouse IgG secondary antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB002). View our protocol for Staining Membrane-associated Proteins.
HEK293 human embryonic kidney cell line transfected with either (A) human VSIG3 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human VSIG3 Monoclonal Antibody (Catalog # MAB9229) followed by ...read more
Recombinant Human VSIG3 protein was serially diluted 2-fold and captured by Mouse Anti-Human VSIG3 Monoclonal Antibody (Catalog # MAB9229) coated on a Clear Polystyrene Microplate (Catalog # DY990). Sheep ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA, Flow, CyTOF-ready
Clone
774208
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB9229U-100UG
Alexa Fluor 405
FAB9229V-100UG
Alexa Fluor 488
FAB9229G-100UG
Alexa Fluor 532
FAB9229X
Alexa Fluor 594
FAB9229T-100UG
Alexa Fluor 647
FAB9229R-100UG
Alexa Fluor 700
FAB9229N-100UG
Alexa Fluor 750
FAB9229S-100UG
Allophycocyanin
FAB9229A
Allophycocyanin/Cy7
FAB9229APCCY7
Biotin
FAB9229B
CoraFluor 1
FAB9229CL1
DyLight 350
FAB9229D
DyLight 405
FAB9229E
DyLight 488
FAB9229K
DyLight 550
FAB9229L
DyLight 594
FAB9229M
DyLight 650
FAB9229W
DyLight 680
FAB9229Y
DyLight 755
FAB9229Z
FITC
FAB9229F
HRP
FAB9229H
Janelia Fluor 525
FAB9229JF525
Janelia Fluor 549
FAB9229I
Janelia Fluor 646
FAB9229J
Janelia Fluor 669
FAB9229JF669
PE
FAB9229P
PE/Atto594
FAB9229PEATT594
PE/Cy5.5
FAB9229PECY55
PE/Cy7
FAB9229PECY7
PerCP
FAB9229C
mFluor Violet 450 SE
FAB9229MFV450
mFluor Violet 500 SE
FAB9229MFV500
mFluor Violet 610 SE
FAB9229MFV610
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB9229-SP
Carrier Free
FAB9229N-100UG
Carrier Free
FAB9229G-100UG
Carrier Free
FAB9229V-100UG
Carrier Free
FAB9229U-100UG
Carrier Free
FAB9229T-100UG
Carrier Free
FAB9229S-100UG
Carrier Free
FAB9229R-100UG
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VSIG3/IGSF11 Antibody (774208) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human VSIG3
Met1-Gly245
Accession # Q5DX21
Specificity
Detects human VSIG3 in direct ELISAs.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • ELISA
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for VSIG3/IGSF11 Antibody (774208) [Unconjugated]

  • Brain and testis-specific immunoglobulin superfamily protein
  • BTIGSF
  • BT-IgSF
  • cancer/testis antigen 119
  • CT119
  • CXADR like 1
  • CXADRL1
  • IGSF11
  • Igsf13
  • immunoglobulin superfamily member 11
  • immunoglobulin superfamily, member 11
  • MGC35227
  • V-set and immunoglobulin domain containing 3
  • V-set and immunoglobulin domain-containing protein 3
  • VSIG3
  • VSIG3brain and testis-specific immunoglobin superfamily protein

Background

VSIG3 (V-set and Ig domain-containing protein 3; also BT-IgSF and IGSF11) is a 52 kDa brain and testis-specific protein that belongs to the IGSF11 family of proteins. It is expressed by neurons, astrocytes and oligodendroglia. VSIG3 is an adhesion molecule that forms Ca-independent homophilic interactions in trans. Human VSIG3 is 413 amino acids (aa) in length. It is a type I transmembrane glycoprotein that contains a 219 aa extracellular domain (ECD). The ECD contains one V-type (aa 23‑136) and one C2-type Ig-like domain (aa 144‑234). Over aa 23‑245, human VSIG3 is 94% aa identical to mouse VSIG3. Two potential splice variants exist in human. Both exhibit a 16 aa substitution for the first 17 aa of the signal sequence, and one contains an additional single Ala substitution for aa 211‑235.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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