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VSIG3/IGSF11 Antibody (2637C) [Unconjugated]

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VSIG3 was detected in immersion fixed paraffin-embedded sections of human colon using Rabbit Anti-Human VSIG3 Monoclonal Antibody (Catalog # MAB92294) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in glandular cells. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
VSIG3 was detected in immersion fixed paraffin-embedded sections of human colon using Rabbit Anti-Human VSIG3 Monoclonal Antibody (Catalog # MAB92294) at 3 µg/mL for 1 hour at room temperature followed by ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC
Clone
2637C
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB92294-SP
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VSIG3/IGSF11 Antibody (2637C) [Unconjugated] Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Human VSIG3 synthetic peptide
Accession # Q5DX21
Specificity
Detects human VSIG3 in direct ELISAs.
Source
N/A
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Purity Statement
Protein A or G purified from cell culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 3-25 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for VSIG3/IGSF11 Antibody (2637C) [Unconjugated]

  • Brain and testis-specific immunoglobulin superfamily protein
  • BTIGSF
  • BT-IgSF
  • cancer/testis antigen 119
  • CT119
  • CXADR like 1
  • CXADRL1
  • IGSF11
  • Igsf13
  • immunoglobulin superfamily member 11
  • immunoglobulin superfamily, member 11
  • MGC35227
  • V-set and immunoglobulin domain containing 3
  • V-set and immunoglobulin domain-containing protein 3
  • VSIG3
  • VSIG3brain and testis-specific immunoglobin superfamily protein

Background

VSIG3 (V-set and Ig domain-containing protein 3; also BT-IgSF and IGSF11) is a 52 kDa brain and testis-specific protein that belongs to the IGSF11 family of proteins. It is expressed by neurons, astrocytes and oligodendroglia. VSIG3 is an adhesion molecule that forms Ca-independent homophilic interactions in trans. Human VSIG3 is 413 amino acids (aa) in length. It is a type I transmembrane glycoprotein that contains a 219 aa extracellular domain (ECD). The ECD contains one V-type (aa 23‑136) and one C2-type Ig-like domain (aa 144‑234). Over aa 23‑245, human VSIG3 is 94% aa identical to mouse VSIG3. Two potential splice variants exist in human. Both exhibit a 16 aa substitution for the first 17 aa of the signal sequence, and one contains an additional single Ala substitution for aa 211‑235.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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