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VPS45 Antibody

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Western Blot: VPS45 Antibody [NBP1-81642] - Analysis in human cell line RH-30.
Immunocytochemistry/ Immunofluorescence: VPS45 Antibody [NBP1-81642] - Staining of human cell line U-2 OS shows positivity in cytoskeleton (microtubules).
Immunohistochemistry-Paraffin: VPS45 Antibody [NBP1-81642] - Staining of human epididymis shows cytoplasmic positivity in glandular cells.
Western Blot: VPS45 Antibody [NBP1-81642] - Analysis in human cell line RT-4.
Western Blot: VPS45 Antibody [NBP1-81642] - Analysis in control (vector only transfected HEK293T lysate) and VPS45 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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VPS45 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: KETTGIVSMVYTQSEILQKEVYLFERIDSQNREIMKHLKAICFLRPTKENVDYIIQELRRPKYTIYFIYFSNVISKSDVKSLAEADEQEVVA
Predicted Species
Mouse (97%), Rat (97%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
VPS45
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
VPS45 Protein (NBP1-81642PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for VPS45 Antibody

  • H1
  • H1VPS45
  • hlVps45
  • h-vps45
  • leucocyte vacuolar protein sorting 45
  • vacuolar protein sorting 45 homolog (S. cerevisiae)
  • vacuolar protein sorting 45A (yeast homolog)
  • vacuolar protein sorting 45A (yeast)
  • vacuolar protein sorting 45A
  • vacuolar protein sorting-associated protein 45
  • VPS45A
  • VPS45AVPS54A
  • VPS45B
  • VSP45
  • VSP45A

Background

FUNCTION: May play a role in vesicle-mediated protein trafficking from the Golgi stack through the trans-Golgi network. SUBUNIT: Interacts with STX6 and ZFYVE20. SUBCELLULAR LOCATION: Golgi apparatus membrane; Peripheral membrane protein. Endosome membrane; Peripheral membrane protein. Note: Associated with Golgi/endosomal vesicles and the trans-Golgi network. TISSUE SPECIFICITY: Ubiquitous; expression was highest in testis and in brain. Detected in every part of the brain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for VPS45 Antibody (NBP1-81642) (0)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol VPS45