![Western Blot: VPS25 Antibody [NB100-2468] - Staining of Rat Kidney lysate (35 ug protein in RIPA buffer) with antibody at 0.5 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/VPS25-Antibody-Western-Blot-NB100-2468-img0001.jpg "Western Blot: VPS25 Antibody [NB100-2468] - Staining of Rat Kidney lysate (35 ug protein in RIPA buffer) with antibody at 0.5 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.")
| Reactivity | Rt, Mu, CaSpecies Glossary |
| Applications | WB, ELISA, IHC |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Concentration | 0.5 mg/ml |
| Immunogen | Peptide with sequence C-QPNVDTRQKQ corresponding to internal region according to NP_115729.1. |
| Predicted Species | Mouse (100%), Canine (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | VPS25 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Western blot: Approx. 22kDa band observed in Rat Kidney lysates (calculated MW of 20.7kDa according to human NP_115729.1). IHC: In paraffin embedded human kidney, shows granular cytoplasmic staining of endothelial cells. Data obtained from the previous batch (different goat). |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Immunogen affinity purified |
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| Publication using NB100-2468 | Applications | Species |
|---|---|---|
| Vaccari T, Bilder D. The Drosophila Tumor Suppressor vps25 Prevents Nonautonomous Overproliferation by Regulating Notch Trafficking. Dev Cell 2005-11-01 [PMID: 16256743] |
Secondary Antibodies |
Isotype Controls |
Research Areas for VPS25 Antibody (NB100-2468)Find related products by research area.
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| Gene Symbol | VPS25 |
| Entrez |
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| Uniprot |
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![Immunohistochemistry-Paraffin: VPS25 Antibody [NB100-2468] - Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.](http://images.novusbio.com/fullsize/NB100-2468_Immunohistochemistry-Paraffin-VPS25-Antibody-NB100-2468-Negative-Control-showing-staining-of-paraffin-embedded-Human-Kidney-with-no-primary-antibody--29320239254028.jpg "Immunohistochemistry-Paraffin: VPS25 Antibody [NB100-2468] - Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.")
![Immunohistochemistry-Paraffin: VPS25 Antibody [NB100-2468] - (6ug/ml) staining of paraffin embedded Human Kidney. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/NB100-2468_Immunohistochemistry-Paraffin-VPS25-Antibody-NB100-2468-6ug-ml-staining-of-paraffin-embedded-Human-Kidney-Heat-induced-antigen-retrieval-with-citrate-buffer-pH-6-HRP-staining--29320239254015.jpg "Immunohistochemistry-Paraffin: VPS25 Antibody [NB100-2468] - (6ug/ml) staining of paraffin embedded Human Kidney. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.")
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