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CHMP6 Antibody

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Western Blot: CHMP6 Antibody [NBP1-83726] - Analysis in control (vector only transfected HEK293T lysate) and CHMP6 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Western Blot: CHMP6 Antibody [NBP1-83726] - Analysis in control (vector only transfected HEK293T lysate) and CHMP6 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: CHMP6 Antibody [NBP1-83726] - Staining of human appendix shows cytoplasmic positivity in germinal center.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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CHMP6 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: QFGNECLNKMHQVMSIEEVERILDETQEAVEYQRQIDELLAGSFTQEDEDAILEELSAITQEQIELPEVPSEPLPEKIPENVPVK
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CHMP6
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
CHMP6 Protein (NBP1-83726PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (86%), Rat (88%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for CHMP6 Antibody

  • charged multivesicular body protein 6
  • chromatin modifying protein 6
  • Chromatin-modifying protein 6
  • FLJ11749
  • Vacuolar protein sorting-associated protein 20
  • Vps20
  • VPS20hVps20

Background

Component of the escrt-iii complex, which is required for multivesicular bodies (mvbs) formation and sorting of endosomal cargo proteins into mvbs. the mvb pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. the escrt-iii complex is probably involved in the concentration of mvb cargo. in the escrt-iii complex, it probably serves as an acceptor for escrt-ii complex on endosomal membranes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CHMP6