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Vitamin D BP Antibody (VDBP/4481)

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Immunohistochemistry-Paraffin: Vitamin D BP Antibody (VDBP/4481) [NBP3-13923] - IHC analysis of formalin-fixed, paraffin-embedded human pancreas. Staining using Vitamin D BP antibody (VDBP/4481) at 2ug/ml in PBS for ...read more
Immunohistochemistry-Paraffin: Vitamin D BP Antibody (VDBP/4481) [NBP3-13923] - IHC analysis of formalin-fixed, paraffin-embedded human renal oncocytoma. Staining using Vitamin D BP antibody (VDBP/4481) at 2ug/ml in PBS ...read more
Protein Array: Vitamin D BP Antibody (VDBP/4481) [NBP3-13923] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Vitamin D BP Antibody (VDBP/4481).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC, MA
Clone
VDBP/4481
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

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Vitamin D BP Antibody (VDBP/4481) Summary

Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14005)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Immunogen
Recombinant fragment (around aa 35-175) of human Vitamin D BP protein (exact sequence is proprietary) (Uniprot: P02774)
Localization
Secreted
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
GC
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Protein Array
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes
Theoretical MW
53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for Vitamin D BP Antibody (VDBP/4481)

  • DBP
  • DBP/GC
  • DBP-MAF
  • DBPVDBG
  • EC 6.3.1.5
  • Gc
  • gc-globulin
  • Gc-MAF
  • GRD3
  • group-specific component (vitamin D binding protein)
  • Group-specific component
  • hDBP
  • VDB
  • VDBG
  • VDBP
  • Vitamin D BP
  • vitamin D-binding alpha-globulin
  • vitamin D-binding protein

Background

Vitamin D Binding Protein is a secreted multifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid and urine, and on the surface of many cell types. In plasma, it carries the vitamin D sterols and prevents polymerization of actin by binding its monomers. DBP associates with membrane bound immunoglobulin on the surface of B lymphocytes and with IgG fc receptor on the membranes of T lymphocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol GC
Uniprot