Home » VEGFR2/KDR/Flk-1 » VEGFR2/KDR/Flk-1 Antibodies » VEGFR2/KDR/Flk-1 [p Tyr1214] Antibody [Unconjugated]
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VEGFR2/KDR/Flk-1 [p Tyr1214] Antibody [Unconjugated]

 (13 Publications)
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Western Blot shows lysates of A431 human epithelial carcinoma cell line untreated (-) or treated (+) with 100 μM pervanadate (PV) for 10 minutes. PVDF membrane was probed with 1 µg/ml of Rabbit Anti-Human Phospho-VEGFR2/KDR/Flk-1 (Y1214) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1766) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for VEGFR2/KDR/Flk-1 at approximately 230 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
Western Blot shows lysates of A431 human epithelial carcinoma cell line untreated (-) or treated (+) with 100 μM pervanadate (PV) for 10 minutes. PVDF membrane was probed with 1 µg/ml of Rabbit Anti-Human ...read more
VEGFR2/KDR/Flk-1 phosphorylated at Y1214 was detected in immersion fixed A431 human epithelial carcinoma cell line untreated (lower panel) or treated (upper panel) with pervanadate using Rabbit Anti-Human ...read more
FoxM1 and RASSF1A expression is inversely co-related in colon cancer tissues. (A) Tissue extranction from both normal and colon cancer patient for monitoring the translational level of FoxM1 and RASSF1A by immunoblot. ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price
Carrier Free
AF1766-SP
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VEGFR2/KDR/Flk-1 [p Tyr1214] Antibody [Unconjugated] Summary

Immunogen
Phosphopeptide containing human VEGFR2/KDR/Flk-1 Y1214 site
Modification
p Tyr1214
Specificity
Detects human VEGFR2/KDR/Flk‑1 in Western blots when phosphorylated at Y1214.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
KDR
Purity Statement
Antigen Affinity-purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
AF1766 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for VEGFR2/KDR/Flk-1 [p Tyr1214] Antibody [Unconjugated]

  • CD309 antigen
  • CD309
  • EC 2.7.10
  • EC 2.7.10.1
  • Fetal liver kinase 1
  • fetal liver kinase-1
  • Flk1
  • Flk-1
  • FLK1tyrosine kinase growth factor receptor
  • KDR
  • kinase insert domain receptor (a type III receptor tyrosine kinase)
  • Kinase insert domain receptor
  • KRD1
  • Ly73
  • Protein-tyrosine kinase receptor flk-1
  • soluble VEGFR2
  • vascular endothelial growth factor receptor 2
  • VEGF R2
  • VEGFR
  • VEGFR2
  • VEGFR-2

Background

VEGFR2 (KDR/Flk-1), VEGFR1 (Flt-1) and VEGFR3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domains and kinase insert domains in their intracellular regions. The expression of VEGFR1, 2, and 3 is almost exclusively restricted to the endothelial cells. These receptors are likely to play essential roles in vasculogenesis and angiogenesis.

VEGFR2 cDNA encodes a 1356 amino acid (aa) residue precursor protein with a 19 aa residue signal peptide. Mature VEGFR2 is composed of a 745 aa residue extracellular domain, a 25 aa residue transmembrane domain and a 567 aa residue cytoplasmic domain. In contrast to VEGFR1 which binds both PlGF and VEGF with high affinity, VEGFR2 binds VEGF but not PlGF with high affinity. The recombinant soluble VEGFR2/Fc chimera binds VEGF with high affinity and is a potent VEGF antagonist.

  1. Ferra, N. and R. Davis-Smyth (1997) Endocrine Reviews 18:4.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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VEGFR2/KDR/Flk-1
[p Tyr1214] Antibody ...
AF1766
VEGFR2/KDR/Flk-1 [p Tyr1214] Antibody [Unconjugated]
Species: Hu
Applications: WB, ICC/IF

Publications for VEGFR2/KDR/Flk-1 Antibody (AF1766)(13)

We have publications tested in 2 confirmed species: Human, Hamster.

We have publications tested in 4 applications: ICC, Immunocytochemistry, TaqMan Protein Assay, Western Blot.


Filter By Application
ICC
(2)
Immunocytochemistry
(1)
TaqMan Protein Assay
(1)
Western Blot
(3)
All Applications
Filter By Species
Human
(6)
Hamster
(1)
All Species
Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using AF1766 Applications Species
Scuruchi, M;Aliqu�, F;Avenoso, A;Mandraffino, G;Vermiglio, G;Minuti, A;Campo, S;Campo, GM;D'Ascola, A; Endocan Knockdown Down-Regulates the Expression of Angiogenesis-Associated Genes in Il-1� Activated Chondrocytes Biomolecules 2023-05-18 [PMID: 37238720] (Immunocytochemistry, Human) Immunocytochemistry Human
Liying Xing, Guanqun Huang, Rongyuan Chen, Lijuan Huang, Juanxi Liu, Xiangrong Ren et al. Critical role of mitogen-inducible gene 6 in restraining endothelial cell permeability to maintain vascular homeostasis Journal of Cell Communication and Signaling 3/1/2023 [PMID: 36284029]
Rodrigo Azevedo Loiola, Miguel García-Gabilondo, Alba Grayston, Paulina Bugno, Agnieszka Kowalska, Sophie Duban-Deweer et al. Secretome of endothelial progenitor cells from stroke patients promotes endothelial barrier tightness and protects against hypoxia-induced vascular leakage Stem Cell Research & Therapy 10/26/2021 [PMID: 34702368]
PN Kouam, GA Rezniczek, IA Adamietz, H Bühler Ionizing radiation increases the endothelial permeability and the transendothelial migration of tumor cells through ADAM10-activation and subsequent degradation of VE-cadherin BMC Cancer, 2019-10-16;19(1):958. 10/16/2019 [PMID: 31619190]
Dong Young Kim, Jeong Ae Park, Yeomyung Kim, Minyoung Noh, Songyi Park, Eunkyung Lie et al. SALM4 regulates angiogenic functions in endothelial cells through VEGFR2 phosphorylation at Tyr1175 The FASEB Journal 9/1/2019 [PMID: 31170000]
Thomas G. Blanchard, Steven J. Czinn, Vivekjyoti Banerjee, Neha Sharda, Andrea C. Bafford, Fahad Mubariz et al. Identification of Cross Talk between FoxM1 and RASSF1A as a Therapeutic Target of Colon Cancer Cancers (Basel) 2/8/2019 [PMID: 30744076]
TG Blanchard, R Lapidus, V Banerjee, AC Bafford, SJ Czinn, H Ahmed, A Banerjee Upregulation of RASSF1A in Colon Cancer by Suppression of Angiogenesis Signaling and Akt Activation Cell. Physiol. Biochem., 2018-07-25;48(3):1259-1273. 2018-07-25 [PMID: 30045022] (Western Blot, Human) Western Blot Human
J Geng, L Wang, M Qu, Y Song, X Lin, Y Chen, M Mamtilahun, S Chen, Z Zhang, Y Wang, GY Yang Endothelial progenitor cells transplantation attenuated blood-brain barrier damage after ischemia in diabetic mice via HIF-1? Stem Cell Res Ther, 2017-07-11;8(1):163. 2017-07-11 [PMID: 28697748] (ICC, Human) ICC Human
Gareth W. Fearnley, Gina A. Smith, Izma Abdul-Zani, Nadira Yuldasheva, Nadeem A. Mughal, Shervanthi Homer-Vanniasinkam et al. VEGF-A isoforms program differential VEGFR2 signal transduction, trafficking and proteolysis Biology Open 5/15/2016 [PMID: 27044325]
DSGOST inhibits tumor growth by blocking VEGF/VEGFR2-activated angiogenesis Oncotarget, 2016-04-19;7(16):21775-85. 2016-04-19 [PMID: 26967562] (Western Blot, Human) Western Blot Human
Show All 13 Publications.

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Bioinformatics

Gene Symbol KDR