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VASP Antibody (OTI4D6) - Azide and BSA Free

Images

 
Western Blot: VASP Antibody (OTI4D6) - Azide and BSA Free [NBP2-74830] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VASP (Right lane) cDNA for 48 hrs and lysed. Equivalent ...read more
Immunocytochemistry/ Immunofluorescence: VASP Antibody (OTI4D6) - Azide and BSA Free [NBP2-74830] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY VASP.
Immunohistochemistry: VASP Antibody (OTI4D6) - Azide and BSA Free [NBP2-74830] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-VASP mouse monoclonal antibody.
Flow Cytometry: VASP Antibody (OTI4D6) - Azide and BSA Free [NBP2-74830] - Analysis of Jurkat cells, using anti-VASP antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Western Blot: VASP Antibody (OTI4D6) - Azide and BSA Free [NBP2-74830] - Analysis of extracts (35ug) from 9 different cell lines by using anti-VASP monoclonal antibody.
Immunohistochemistry: VASP Antibody (OTI4D6) - Azide and BSA Free [NBP2-74830] - Staining of paraffin-embedded Human endometrium tissue using anti-VASP mouse monoclonal antibody.
Immunohistochemistry: VASP Antibody (OTI4D6) - Azide and BSA Free [NBP2-74830] - Staining of paraffin-embedded Human liver tissue using anti-VASP mouse monoclonal antibody.
Immunohistochemistry: VASP Antibody (OTI4D6) - Azide and BSA Free [NBP2-74830] - Staining of paraffin-embedded Human lymph node tissue using anti-VASP mouse monoclonal antibody.
Immunohistochemistry: VASP Antibody (OTI4D6) - Azide and BSA Free [NBP2-74830] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-VASP mouse monoclonal antibody.
Immunohistochemistry: VASP Antibody (OTI4D6) - Azide and BSA Free [NBP2-74830] - Staining of paraffin-embedded Human lymphoma tissue using anti-VASP mouse monoclonal antibody.
Flow Cytometry: VASP Antibody (OTI4D6) - Azide and BSA Free [NBP2-74830] - Analysis of Hela cells, using anti-VASP antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, CyTOF-ready
Clone
OTI4D6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

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VASP Antibody (OTI4D6) - Azide and BSA Free Summary

Immunogen
Full length human recombinant protein of human VASP(NP_003361) produced in 293T Cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
VASP
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-2000
Theoretical MW
39.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for VASP Antibody (OTI4D6) - Azide and BSA Free

  • vasodilator-stimulated phosphoprotein
  • VASP

Background

Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG. VASP shares a limited degree of homology with the amino terminus of WASP, which is frequently mutated in Wiskott-Aldrich syndrome (WAS) patients. An established substrate of cAMP and cGMP dependent kinases, VASP is phosphorylated on a regulatory serine residue 157 and localizes to focal adhesions, microfilaments and highly active regions of the plasma membrane. VASP is highly expressed in human platelets and, like WASP, may play a role in cytoskeletal organization.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol VASP